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Optical and thermal applications in grapevine (Vitis vinifera L.) research: an overview and some novel approaches.

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OPTICAL AND THERMAL APPLICATIONS IN GRAPEVINE

(VITIS VINIFERA L.) RESEARCH – AN OVERVIEW AND SOME

NOVEL APPROACHES

A. E. Strever*, D. Bezuidenhout**, R. Zorer***, T. Moffat*, J. J. Hunter**** &* * Department of Viticulture and Oenology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa, E-mail: aestr@sun.ac.za

** Software Engineering, DynaSoft, 8 Schreiner Close, Strand, 7140, South Africa.

*** GIS and Remote Sensing Unit, Biodiversity and Molecular Ecology Department – DBEM, IASMA

Research and Innovation Centre, Fondazione Edmund Mach, Trento, Italy.

**** ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599 Stellenbosch, South Africa.

Abstract:

In this article, some optical and thermal applications in grapevine research are reviewed and methods to quantify the light and temperature regime around a grape bunch are discussed. This includes temperature measurement techniques (thermocouples and thermal imaging) as well as methods to quantify light quantity (hemispherical photography) as well as light quality (spectroradiometric applications) around a grape bunch. Available methods for real-time quantification of grapevine canopy size and density for application in variable rate technology sprayers are discussed, and a novel and simple approach of using opto-electronic sensors for quantification of grapevine canopy thickness and density is presented. Some scientific as well as practical applications of these individual techniques are discussed, along with their potential integration to improve knowledge of the grape bunch and canopy interaction with the environment.

Key words: Optical, thermal, Vitis vinifera, hemispherical photography, gap fraction.

1. INTRODUCTION

The wine industry plays an important role in South-Africa, growing in 2008 to an annual contribution to the economy of R26.2-billion or 2.2% of the gross domestic product (GDP) [1]. Wine and grapevine-related research contributes significantly to this thriving industry. Wine research is closely related to the monitoring of the grapevine and its environment. Novel techniques for quantifying and understanding the environment around a grape bunch are needed in research, especially due to the variable nature of a vineyard and its product [2, 3]. When considering sensor applications in grapevine research, an important consideration is the spatial and temporal scale at which these measurements are conducted, in and around the vineyard. On a macro-scale we can consider the interpolation of land surface temperature data from satellites using geographical information systems [4, 5]. This approach is also dependent on a sensor network for calibration purposes and can include satellite/aerial remote sensing techniques. On a meso-scale (on-farm or between-vineyard scale) we mostly deal with remote sensing technology (which can also be performed on proximal or canopy scale) as well as weather stations that are used to make meteorological observations. On a micro scale, measurements are made within or around the grapevine canopy, within or around a bunch or even within or around a single berry or leaf (also referred to as nano-scale measurements). If an analogy is to be made between a plant and sensors, it could be reasoned that a plant is an array of sensors able to react to light quantity

and quality on a spatial and temporal basis, leading to physiological reactions affecting its growth, reproductive functionality and commercial quality. A complicating factor when using sensors in grapevine research is that we are dealing with effects of light quality and quantity on leaves and fruit in a spatially complex and temporally changing environment. The ideal sensor arrangement would be able to quantify, for instance in an imaging spectrometer arrangement, light quantity and quality over time in various positions within the canopy. Unfortunately, such an arrangement would be extremely expensive, especially if replication is required. For the moment at least, we can rely on sensors and modelling to get close to this goal, but certainly electronics and sensor technology could help in studying these interactions in a more efficient way, be it in terms of lower cost or higher resolution of the data generated. Another issue in using sensors in grapevine research is that sometimes care is not taken to understand the calibration conditions and measuring limits of the instruments. This is especially true when conducting light measurements, where the calibration of for instance pyranometers is done in high light environments comparable to the conditions that the sensor would encounter under clear skies. Measurements are then done inside canopies under low light conditions with different wavelength distribution and it is accepted that the calibration is still valid. While we are looking for improved solutions, this paper aims to expose the electronic engineering world to some of the applications and challenges of grapevine (and certainly also other fruit) research related to light and temperature Abstract: In this article, some optical and thermal applications in grapevine research are reviewed

and methods to quantify the light and temperature regime around a grape bunch are discussed. This includes temperature measurement techniques (thermocouples and thermal imaging) as well as methods to quantify light quantity (hemispherical photography) as well as light quality (spectroradiometric applications) around a grape bunch. Available methods for real-time quanti cation of grapevine canopy size and density for application in variable rate technology sprayers are discussed, and a novel and simple approach of using opto-electronic sensors for quanti cation of grapevine canopy thickness and density is presented. Some scienti c as well as practical applications of these individual techniques are discussed, along with their potential integration to improve knowledge of the grape bunch and canopy interaction with the environment.

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an temperatur re is shown

t one hour c rature can bility.

mal imager set rape bunch tem

ograph of the bunch in a no ram of “tempe h at 11:00 and quantity/qualit hotography: W to grape ripe o sunlight is of radiation o th potential temperature b crease as sol

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wind speed bunches can pictures (Fig direction, ca different tim season on th use a speci Corp., Toky Corp., Tokyo using Gap Li compute can azimuth reso incident direc Figure 5: H south ori indicated. S ac Spectroradio satellite, can distribution reflected, tra elements. W can aid in radiation pe photography time, as we latitude/long also relevant is affected in of light inte functioning t between diffe 3. CANO The gap fra measurement studies, but spray penet parameters c analysis [12] prone to hum way of usin sunfleck patt this is also decrease [8]. n be compute gure 5) in ord anopy gap fra mes of the da he quantity of

ial lens (Fish o, Japan) and o, Japan) comb ight Analyser nopy gap fra olution as we ct and diffuse

Hemispherical iented vineyar un movement cross the grid

ometry can be nopy, leaf or of direct or ansmitted or While pyranom assessing t enetrating in can aid in ll as its pote itude as well t to know how n terms of spe ercepted by l through photo ferent wavelen OPY SIZE/DE action as wel ts are relev also when co tration into can be measu ], but this is a man error. It i ng a ruler or tern undernea time-of-day The light m ed by means der to assess action and tre ay, or days f light received heye Convert d camera (Co bination, and software ver. action at 10-d ell as the ann

radiation. l photograph t rd row, with c t is indicated b (courtesy, R. e used at all s bunch) to a r diffuse ligh absorbed by meters or qua otal photosy nto a canop modelling to ential reaction as row directi w light penetra ectral distribut eaves or frui on receptors ab ngths of light [ ENSITY QUA l as leaf lay vant in cano onsidering lig a grapevine ured by way laborious me is also possib r photographi ath a vineyard

and row dir

microclimate a of hemisphe the effect of ellis height du within a gro d. It is possib ter FC-E8 N oolpix 995, N analyse the re 2·0 [9] in ord degree zenith nual percentag taken in a nort coordinate grid by the curved Zorer). cale levels (a assess the spe

ht and also y different ca antum radiom ynthetically a py, hemisphe otal radiation n to a chang ion. It is, how ating into a ca tion, as the qu it can affect ble to differen [10, 11]. ANTIFICATIO er number (L opy microcli ght, air as we e canopy. T of point qu ethod which is ble to quantify ic techniques) d canopy [12] rection depen above erical f row uring owing ble to Nikon Nikon esults der to h and ge of th-d line aerial, ectral light anopy meters active erical over ge in wever, anopy uality their ntiate ON LLN) imate ell as These uadrat s also y (by ) the ], but ndent. Opto simp 3.1 O Seve cano tech proc simi they Gree wav tech cond proc We diod trans cano and oper desig cano The conc devi addr such sign pote the pene wast appl throu As throu thick two dista The grap way shoo vine Som whic quite fract were muc o-electronic s plify/automate Opto-electron eral electronic opy size/den hnologies and cessing. Wav ilar to those d y can also be enseeker™). T vebands to hniques have dition [13], cessing to be u developed a s des and ph smittance in b opy (Figure 6 inexpensive, ration (Figure gned to run on opy, which is n test system cept, and coul ice after mec ress the beam h a simple setu nal processing entially a spra canopy. The etration throug tage, and spr lication wher ugh the whole the penetratio ugh a canop kness as well optical (inf ance sensor on test run wa pevines were y of early sh ots were remo es in-between me results of a ch it is evide e well compa tion results, b e erratic. Th ch higher gap f sensors there e these types o nic sensors c sensors are a sity quantifi d requiring d elength-specif eployed on sa deployed at These sensors assess plan also been but this app useful. simple gap fra hoto transist beams perpend and Figure 7 , but needs e 8). Also t n a pre-install not optimal fo was, howeve ld be adapted chanical stab m centring issu up was to a) m g and to c) ay droplet’s a logic is that gh the canopy ray nozzles c re potential e canopy. on of light a py would b , an experime frared) and nto the setup ( as performed subjected to hoot removal

oved for three unaltered as c a trial run are ent that the u ared to the ha ut the optical he shoot-thinn fraction than t fore offer po of measureme available to ai cation utilisi different typ fic sensors a atellite/aerial p a proximal l s use the red nt health. used to as proach requir action sensor t tors to ass dicular to the ). The setup i some alignm the test syste led wire over or extensive m er, designed a d to be mounte ilisation, whi ue. The goals minimise cost mimic a be ability to pene t in forced sp y would poten could be adap penetration as well as sp be dependent

ent was done one ultrason (Figure 9). d on canopie o canopy man (Figure 10). e grapevines, controls. shown in Fig ultrasonic bea and measurem (infrared) dis ned grapevine the unmodifie ossibilities to ents. id in vineyard ing different es of signal are available, platforms, but level (i.e. the d and infrared Photographic ssess canopy res extensive that uses laser sess canopy surface of the is very simple ment prior to em has been the grapevine measurements. as a proof of ed on a spray ich will also of designing t, b) minimise eam, or then etrate through pray systems, ntially lead to pted to lower could occur pray droplets t on canopy by mounting nic low-cost es where the nipulation by Half of the leaving three gure 11, from am performed ments and gap stance sensors es showed a ed vines. o d t l , t e d c y e r y e e o n e . f y o g e n h , o r r s y g t e y e e m d p s a Figure 5: Hemispherical photograph taken in a

north-south oriented vineyard row, with coordinate grid indicated. Sun path is indicated by the curved line across

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Figure 6: C Figure 7: D photo Figure 8: Be Figure 9: sensors insta Canopy gap fr Detail of canop transistor setu eam centring t h Ultrasonic an alled on the la thi fraction analys py gap analyse up inside the s to align it to th holes. nd infrared (op ser beam track ickness. ser component er laser beam sensor arms. he photo trans ptical) distanc k to assess can ts. and sistor ce nopy F thic of c c grap 4. One tech the Afri exec !60 !40 !20 0 20 40 60 !60 !40 !20 0 20 40 60 Figure 10: Unm (right) Figure 11: T ckness; middl canopy thickne gaps in darke orrespond to g pevines 7, 8 an a SENSOR A example of a hnology is the Department ca, and the cuted by W 4 4 5 5 6 4.0 5.0 6 30"per."Mo 30"per."Mo modified (left grapevines us op – hand me le – ultrasonic ess; bottom – er pixels. The grapevines 4, nd 9 (unmodif and 12 (shoot APPLICATIO VITICU a macro/meso e initiative “G of Agricultu European sp WaterWatch 6 7 7 8 .0 7.0 8.0 ov."Avg."(Optical"W) ov."Avg."(USonic"E) t) or early sho sed in test setu

easurements of c and optical m gap fraction m numbers on th 5 and 6 (shoo fied) and grap t thinned). ONS IN SUST ULTURE scale applicat Grapelook”, c ure, Western pace agency (Netherlands 8 9 9 10 10 West"facin (cm) 0 9.0 10.0 30"per."Mov oot thinned up. f canopy measurement map showing he x-axis ot thinned), pevines 10, 11 TAINABLE tion of sensor co-funded by Cape, South (ESA). It is s) and the 11 11 12 12 ng East"Facing (cm) 11.0 v."Avg."(Optical"E) r y h s e

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University of Kwa-Zulu Natal in South Africa, with the aims of increasing water use efficiency in vineyards, promoting sustainable optimal resource utilization, reducing input costs (i.e. fertilizers) and protecting the environment [14]. The system provides an online interface where grape producers can access information such as leaf area index, crop coefficients, aspect, actual evapotranspiration, evapotranspiration deficit and biomass water use efficiency on maps utilising a Google Earth™ image as background.

An example of sensor applications for sustainable agriculture/viticulture on the meso-scale is the use of variable rate spraying. To this end, the method of assessing canopy dimensions/density real-time is of interest. Several options are available, including multi-band type sensors, photographic techniques, ultrasonic or infrared distance sensors, laser gap fraction analysis or LIDAR. Even though the gap fraction analysis system looks promising to use as a low-cost alternative, canopy profiling through the use of LIDAR would probably still offer the best way to combine canopy profiling and gap fraction analysis into one sensor. It has already shown promise for use in spray technology [15]. Preliminary results from some of these studies demonstrate reduction in spray drift, reduction in cost of application (savings on chemicals, but also less refill cycles) and therefore also reduced impact on the environment [16].

5. CONCLUSIONS AND FUTURE WORK In combination, the technologies that are described in this paper can be powerful drivers to model berry temperature, as already proposed [17]. Furthermore, ways to profile and describe a grapevine canopy and therefore the bunch environment better and with more sampling points (such as when using LIDAR) can help us to better understand and quantify the variability that is often measured in grape temperature or light interception, and perform better measurements to compliment research on the microclimate level.

It is also necessary to find ways to describe the grapevine canopy light and temperature environment better, in order to add value to regional/macro scale analyses such as those performed when satellite data are interpolated. This could improve current available decision making tools by incorporating meso- and micro-scale variability in temperature/light into existing models and to set up new models for the future.

6. REFERENCES

[1] Anonymous, "South-African wine industry 'driving jobs, GDP'", www.southafrica.info, 2010.

[2] A. Strever, "A study of within-vineyard variability with conventional and remote sensing technology.", MSc(Agric)Viticulture Thesis, Stellenbosch University, 2003.

[3] J. J. Hunter, E. Archer, and C. G. Volschenk, "Vineyard management for environment valorisation", in Eighth International Terroir

Zoning Congress, Soave, (Verona), Italy, 2010,

pp. 7-3 to 7-15.

[4] R. Zorer, L. Delucchi, M. Neteler, and G. Nicolili, "Development of a GRASS-GIS application for the characterization of vineyards in the Province of Trento (Poster)", in VIII

International Terroir Congress Soave, (Verona),

Italy, 2010.

[5] R. Zorer, D. Rocchini, L. Delucchi, F. Zottele, F. Meggio, and M. Neteler, "Use of multi-annual MODIS land surface temperature data for the characterisation of the heat requirements for grapevine varieties", in Multitemp 2011, 2011. [6] O. M. Grant, Å. u. Tronina, H. G. Jones, and M.

M. Chaves, "Exploring thermal imaging variables for the detection of stress responses in grapevine under different irrigation regimes",

Journal of Experimental Botany, vol. 58, pp.

815-825, 03/01 2007.

[7] M. Stoll, H. R. Schultz, and B. Berkelmann-Loehnertz, "Exploring the sensitivity of thermal imaging for Plasmopara viticola pathogen detection in grapevines under different water status", Functional Plant Biology, vol. 35, pp. 281-288, 2008.

[8] R. Zorer, T. Cobelli, T. Tomasi, L. Zulini, and M. Bertamini, "Effect of temperature and light availability on ripening of Vitis vinifera L. cv. Chardonnay.", in XIV International GESCO

Viticulture Congress, Geisenheim, Germany,

2005, pp. 319-325.

[9] G. W. Frazer, C. D. Canham, and K. P. Lertzman, "Gap Light Analyzer (GLA), Version 2.0: Imaging software to extract canopy structure and gap light transmission indices from true-colour fisheye photographs, user manual and program documentation". : Simon Fraser University, Burnaby, British Columbia & the Institute of Ecosystem Studies, Millbrook, New York, 1999.

[10] R. E. Smart, S. M. Smith, and R. V. Winchester, "Light Quality and Quantity Effects on Fruit Ripening for Cabernet Sauvignon", American

Journal of Enology and Viticulture, vol. 39, pp.

250-258, 1988.

[11] H. Smith and G. C. Whitelam, "Phytochrome, a family of photoreceptors with multiple physiological roles", Plant, Cell & Environment, vol. 13, pp. 695-707, 1990.

[12] R. Smart and M. Robinson, "Sunlight into wine: A handbook for winegrape canopy management". Adelaide: Winetitles, 1991. [13] H. Bjurström and J. Svensson, "Assessment of

grapevine vigour using image processing", Master's thesis, Linköping University, 2002. [14] C. Postimus, "The intelligent pixel - helping the

farmer: water use efficiency", SABI magazine, vol. 3, pp. 10-11, 2011.

[15] J. Llorens, E. Gil, J. Llop, and A. Escolà, "Ultrasonic and LIDAR Sensors for Electronic

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Canopy Characterization in Vineyards: Advances to Improve Pesticide Application Methods", Sensors, vol. 11, pp. 2177-2194, 2011.

[16] J. Llorens, E. Gil, J. Llop, and A. Escolà, "Variable rate dosing in precision viticulture: Use of electronic devices to improve application efficiency", Crop Protection, vol. 29, pp. 239-248, 2010.

[17] G. Cola, O. Failla, and L. Mariani, "BerryTone—A simulation model for the daily course of grape berry temperature", Agricultural

and Forest Meteorology, vol. 149, pp.

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