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Alpha S1-casein polymorphisms in camel (Camelus dromedarius) and descriptions of biological active peptides and allergenic epitopes

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24 July 2021

Original Citation:

Alpha S1-casein polymorphisms in camel (Camelus dromedarius) and descriptions of biological active peptides and allergenic epitopes

Published version:

DOI:10.1007/s11250-016-0997-6

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Open Access

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This is the author's manuscript

(2)

This full text was downloaded from iris - AperTO: https://iris.unito.it/

iris - AperTO

University of Turin’s Institutional Research Information System and Open Access Institutional Repository This is the author's final version of the contribution published as:

Erhardt, Georg; Shuiep, El Tahir Salih; Lisson, Maria; Weimann, Christina; Wang, Zhaoxin; El Zubeir, Ibtisam El Yas Mohamed; Pauciullo, Alfredo. Alpha S1-casein polymorphisms in camel (Camelus dromedarius) and

descriptions of biological active peptides and allergenic epitopes. TROPICAL ANIMAL HEALTH AND PRODUCTION. 48 (5) pp: 1-9.

DOI: 10.1007/s11250-016-0997-6

The publisher's version is available at:

http://link.springer.com/content/pdf/10.1007/s11250-016-0997-6

When citing, please refer to the published version.

Link to this full text: http://hdl.handle.net/

(3)

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Alpha S1­casein polymorphisms in

camel (Camelus dromedarius) and

descriptions of biological active

peptides and allergenic epitopes

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Abstract

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Keywords

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Introduction

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Material and methods

Milk and blood samples

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Isoelectric focusing of milk proteins

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Molecular analyses: reverse transcription, PCR

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Molecular analyses: reverse transcription, PCR

amplification, and sequencing

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Development of a DNA­based test

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Identification of IgE­binding epitopes and bioactive

peptides

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Statistical analyses

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Results and discussion

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Results and discussion

IEF and allele frequencies

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No. Location Ecotype N

Genotype

frequencies (%) Allele frequencies

AA AC AD A C D

 (O6KXDN /DKDRL        

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Sequencing of cDNA and genomic DNA

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References

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