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Clinical Interventions in Aging
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http://dx.doi.org/10.2147/CIA.S74477
A very rare case of HPV-53-related cervical cancer, in a 79-year-old woman
with a previous history of negative Pap cytology [corrigendum]
Journal name: Clinical Interventions in Aging
Article Designation: Corrigendum
Year: 2014
Volume: 9
Running head verso: Zappacosta et al
Running head recto: A very rare case of HPV-53-related cervical cancer
DOI: http://dx.doi.org/10.2147/CIA.S74477
Zappacosta R, Lattanzio G, Viola P, Ianieri MM, Gatta DMP,
Rosini S. Clin Interv Aging. 2014;9:683–688.
On page 686, Figure 3 originally contained one panel and
incorrect heading and notes. The authors have provided a
new Figure 3 and a new legend.
Figure 3 real-time PCr assay performed on tissues (invasive cervical cancer, CIn2+ lesion adjacent to invasive neoplasia, metastatic lymph nodes, non-metastatic lymph
nodes, and thyroid), and on liquid-based urinary samples.
Notes: Anyplex II HPV28 detection assay simultaneously detects 28 HPV genotypes. β-globin gene is used as a positive endogen control to identify processed specimens containing
substances that may interfere with PCR amplification. Thyroid tissue was used as an external HPV-negative control. (A) Invasive cervical cancer showing positive result for HPV-53.
(B) CIn2+ lesion, in which positive results for HPVs 16, 35, 39, 40, 53, 54, 59, 61, 68 and 82, have been found. (C) metastatic lymph nodes showing HPV-53 positive result.
(D) non-metastatic lymph nodes testing as HPV-negative. (E) Thyroid tissue testing as HPV-negative. (F) urinary samples in which HPV-53 has been found. (G) melting curve of HPV-53
control included in the PC3 mix of set B demonstrating the melting curve shape and Tm. Melting profile of samples, in which HPV-53 has been found, showed a Tm of 76.5°C.
Abbreviations: CIn2+, cervical intraepithelial neoplasia grade 2-or-worse; -d(RFU/dT), change in rate of fluorescence units against temperature; IC, internal control; PC3 mix
of set B, positive control of set B consisting of a mix of pathogens including those of HPVs 73, 53, 70, 6, and 11; Tm, melting temperature; HPV, human papillomavirus.
–d(RFU)/dT
