Micrometastasis
Cancer Metastasis – Biology and Treatment
VOLUME 5
Series Editors
Richard J. Ablin, Ph.D., Innapharma, Inc., Park Ridge, NJ, U.S.A.
Wen G. Jiang, M.D., University of Wales College of Medicine, Cardiff, U.K.
Advisory Editorial Board
Harold F. Dvorak, M.D.
Phil Gold, M.D., Ph.D.
Ian R. Hart, Ph.D.
Hiroshi Kobayashi, M.D.
Robert E. Mansel, M.S., FRCS.
Marc Mareel, M.D., Ph.D.
Titles published in this Series are:
Volume 1: Cancer Metastasis, Molecular and Cellular Mechanisms and Clinical Intervention.
Editors: Wen G. Jiang and Robert E. Mansel.
ISBN 0-7923-6395-7
Volume 2: Growth Factors and Receptors in Cancer Metastasis.
Editors: Wen G. Jiang, Kunio Matsumoto, and Toshikazu Nakamura.
ISBN 0-7923-7141-0
Volume 3: Cancer Metastasis – Related Genes.
Editor: Danny R. Welch ISBN 1-4020-0522-9
Volume 4: Proteases and Their Inhibitors in Cancer Metastasis.
Editors: Jean-Michel Foidart and Ruth J. Muschel ISBN 1-4020-0923-2
Micrometastasis
Edited by
Klaus Pantel
Director, Institute of Tumour Biology
University Hospital Hamburg-Eppendorf, Germany
KLUWER ACADEMIC PUBLISHERS
NEW YORK, BOSTON, DORDRECHT, LONDON, MOSCOW
eBook ISBN: 0-306-48355-6 Print ISBN: 1-4020-1155-5
©2005 Springer Science + Business Media, Inc.
Print ©2003 Kluwer Academic Publishers
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TABLE OF CONTENTS
Preface ...vii Klaus Pantel
List of Contributors ...ix Chapter 1 ...1
Technical Aspects of the Detection of Disseminated Tumour Cells by Molecular Methods
William H. Krüger
Chapter 2 ...19 RNA/DNA Based Detection of Minimal Residual Head and
Neck Cancer Ruud H. Brakenhoff
Chapter 3 ...47 Detection and Characterisation of Occult Metastatic
Cells in Bone Marrow of Breast Cancer Patients:
Implications for Adjuvant Therapy
Stephan Braun, Volkmar Müller and Klaus Pantel
Chapter 4 ...67 Prognosis of Minimal Residual Disease in Bone Marrow,
Blood and Lymph Nodes in Breast Cancer Debra Hawes, A. Munro Neville and Richard J. Cote
Chapter 5 ...87 Detection, Isolation and Study of Disseminated Prostate
Cancer Cells in the Peripheral Blood and Bone Marrow Jesco Pfitzenmaier, Robert L. Vessella, William J. Ellis and Paul H. Lange
Chapter 6 ...117 Early Disseminated Tumour Cells in Operable Non-Small
Cell Lung Cancer Bernward Passlick
Chapter 7 ...127 Prognostic Value of Minimal Residual Disease in
Esophageal Cancer
Peter Scheuemann, Stefan B. Hosch and Jacob R. Izbicki
v
Chapter 8 ...139 Clinical Relevance of Tumor Cell Dissemination in
Colorectal, Gastric and Pancreatic Carcinoma Ilka Vogel and Holger Kalthoff
Chapter 9 ...173 Minimal Residual Disease in Melanoma
Petra Goldin-Lang and Ulrich Keilholz
Index ...185
vi
PREFACE
Distant metastases are the main cause of cancer-related death. The onset of the metastatic process can now be assessed in cancer patients by the use of sensitive immunocytochemical and molecular methods that allow the identification of sin- gle disseminated carcinoma cells or small tumor cell clusters in regional lymph nodes, peripheral blood or distant organs.
The current assays for detection of micrometastatic tumor cells may be used to improve tumor staging with potential consequences also for subsequent adjuvant therapy. Another promising clinical application is monitoring the response of micrometastatic cells in blood and bone marrow to adjuvant thera- pies, which, at present, can only be assessed retrospectively after an extended period of clinical follow-up. Moreover, tools recently established in several lab- oratories allow us to obtain further insights into the phenotype and genotype of micrometastases. Identification of the molecular determinants of micrometastat- ic cells may help to design new strategies to detect and eliminate minimal resid- ual cancer.
In this book, leading experts in the area of micrometastasis research pro- vide an overview that summarizes the current state of research on micrometasta- tic disease in patients with solid tumors. In each chapter, the technical aspect as well as clinical relevance of micrometastasis detection is discussed.
I hope the knowledge provided in this book will help the reader to under- stand the importance of this rather new field of clinical cancer research.
Professor Dr. Klaus Pantel Editor
vii
LIST OF CONTRIBUTORS
Ruud H. Brakenhoff. Section Tumour Biology, Department of Otolaryngology/ Head-Neck Surgery, Vrije Universiteit Medical Center
Stephan Braun. Universitätsklinik für Frauenheilkunde, Leopold-Franzens-Univerrsität, Anichstrasse 35, A-6020 Innsbruck, Austria
Richard J. Cote. Keck School of Medicine at the University of Southern California/Kenneth Norris Comprehensive Cancer Center, Los Angeles, California, USA
William J. Ellis. Department of Urology, University of Washington Medical School, Seattle, USA
Petra Goldin-Lang. Department of Medicine III, University Hospital Benjamin Franklin, Free University Berlin, Hindenburgdamm 30, 12200 Berlin, Germany
Debra Hawes. Keck School of Medicine at the University of Southern California/Kenneth Norris Comprehensive Cancer Center, Los Angeles, California, USA
Stefan B. Hosch. Department of General and Thoracic Surgery, Universitätsklinikum Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany
Jacob R. Izbicki. Department of General and Thoracic Surgery, Universitätsklinikum Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany
Holger Kalthoff. Molecular Oncology Research Group, Department for General and Thoracic Surgery, University Hospital of Schleswig-Holstein, Campus Kiel, Germany
Ulrich Keilholz. Department of Medicine III, University Hospital Benjamin Franklin, Free University Berlin, Hindenburgdamm 30, 12200 Berlin, Germany
William H. Krüger. Internal Medicine C - Haematology/Oncology, Ernst-Moritz-Arndt- University, Greifswald, Germany
Paul H. Lange. Department of Urology, University of Washington Medical School, Seattle, USA
Volkmar Müller. Institut für Tumorbiologie, Klinik für Frauenheilkunde, Universitätsklinikum Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany
A. Munro Neville. Ludwig Institute for Cancer Research, London, UK
Klaus Pantel. Institut für Tumorbiologie, Universitätsklinikum Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany
ix
Bernward Passlick. Department of Surgery, Division of Thoracic Surgery, University of Munich, Germany
Jesco Pfitzenmaier. Department of Urology, University of Washington Medical School, Seattle, USA
Peter Scheuemann. Department of General and Thoracic Surgery, Universitätsklinikum Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany
Robert L. Vessella. Department of Urology, University of Washington Medical School, Seattle, USA
Ilka Vogel. Molecular Oncology Research Group, Department for General and Thoracic Surgery, University Hospital of Schleswig-Holstein, Campus Kiel, Germany
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