Andrea Bassi, Bologna, Oct. 22-2013 Rynaxypyr & Indoxacarb FD Coordinator, EMEA

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Andrea Bassi, Bologna, Oct. 22-2013 Rynaxypyr & Indoxacarb FD Coordinator, EMEA

Coragen ^® (Rynaxypyr ^® ) peculiarities

& use in an IPM-based PTM program.

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Potato Tuber Moth meeting - Bologna 2013

- What Coragen ^® is

- What it brings to PTM control

(3)

25.10.2013

PTM (internal feeder)

The ability to develop as a leafminer

The ability to develop in the tuber

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25.10.2013

Distribution

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In the EU, the Registration project on PTM started in 2008

Rynaxypyr (Coragen ^® ) - 1st registration obtained

Indoxacarb (Avaunt ^® ) – Registration pending

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10/25/201309.

02 2011

6 Tradename Chemical name Chemical class Mode of action (MoA)

MoA Group

Steward

^®

Avaunt

^®

Indoxacarb Oxadiazine Sodium-channel

blocker 22A

Coragen

^®

Chlorantraniliprole

(Rynaxypyr®) Anthralic-Diamide Ryanodine-receptor

modulator 28

DuPont ^® Insecticide Portfolio for Potato

Tradename Chemical name Chemical class Mode of action (MoA)

MoA Group

Coragen

^®

Chlorantraniliprole

(Rynaxypyr®) Anthralic-Diamide Ryanodine-receptor modulator

28

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25.10.2013

What Coragen ^® (Rynaxypyr ^® ) is?

The 1st insecticide acting on insect muscles Discovered by Dupont in June 2001

Sold in the EU since 2008, in major crops

Annex 1 included Oct. 14-2013

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Nomenclature

Common name: Chlorantraniliprole Chemical class: Anthranilic diamide Code number: DPX‐E2Y45

Trademark: Rynaxypyr™

Molecular formula: C

₁₈

H

₁₄

BrCl

₂

N

₅

O

₂

Structural formula:

Molecular weight: 483.15 g/mole

Vapour pressure (20

^o

C): 6.3 × 10‐12 Pa

Part. Coeff. oct/w (Log P

_ow

) (20

^o

C): 2.76 (distilled water) Solubility in water (20

^o

C): 1.0 mg/L

NH NH

O

N N

N Br

Cl Cl

C H₃

CH₃ NH NH

O

N N

N Br

Cl Cl

C H₃

CH₃

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Ryanodine Receptor Modulators

Rynaxypyr new Mode of Action

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Foliar feeders

Fruit borers

Pinworms

Activity on all lepidopteran pests

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25.10.2013

Low impact to natural enemies and pollinators

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A new activity profile

Ovi-larvicidal (neonate die after hatching) Larvicidal (contact and ingestion)

Adult (population reduction)

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25.10.2013

Coragen ^® tested on PTM in field trials in Spain, Italy, Portugal, Greece, Turkey

Results indicate excellent performance on PTM with 2-4 applications.

Normally 20-40% above commercial standards.

Laboratory/semi-field trials helped to clarify

individual aspects of Coragen activity on PTM.

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Overall levels of P. operculella attack and control by treatment, with 50%, 80% and 95% confidence intervals (color shades)

L. Sannino et. al. 2012

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25.10.2013

Small-plot trials

Normally the sprayate cannot wet the tuber portions in the ground.

In particular, nothing can impede an adult

female to lay eggs on a tuber in a nearby plot.

Once an egg is laid on the tuber, it cannot be reached and the larvae will develop safely.

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Ergo (caution)

The egg-laying in unprotected, exposed tuber portions can create a bias in small-plot trials.

Much less in full-scale, commercial applications.

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İZMİR-Ödemiş (Küçükavulcuk village), Aegean Region/TURKEY Year : 2008 ‐ Cultivar : Malabel ‐ Contractor: TARSAV

3 Applications: 16.09/26.09/6.10.2008 Observations: 26.09/6.10/16.10.2008

Commercial Name

Formulation Type

A.I.name Concentration %

Dosage (product) CORAGEN^® 20 SC SC Chlorantraniliprole, 200 g/l 100 ml/ha

125 ml/ha

150 ml/ha

CORAGEN^® 20 SC+ SC Chlorantraniliprole, 200 g/l+ 100 ml/ha+0.1%

Trend^® 90 EC Alcohol ethoxylate,%90 125 ml/ha+0.1%

150 ml/ha+0.1%

AVAUNT^® SC Indoxacarb, 150 g/l 300 ml/ha

400 ml/ha

500 ml/ha

AVAUNT^® SC Indoxacarb, 150 g/l 300 ml/ha+0.1%

Trend^® 90 EC Alcohol ethoxylate,%90 400 ml/ha+0.1%

500 ml/ha+0.1%

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25.10.2013

İZMİR-Ödemiş (Küçükavulcuk village), Aegean Region/TURKEY Table 1. Overall results

First counting

Second counting

Third counting

CORAGEN^® 20 SC (100 ml/ha) 85.30% c 87.02% c 93.70% c CORAGEN^® 20 SC (125 ml/ha) 94.57% abc 96.94% ab 98.81% ab CORAGEN^® 20 SC (150 ml/ha) 98.44% a 98.53% ab 100.00% a CORAGEN^®20 SC+Trend^®90 (100

ml/ha+0.1%)

90.73% bc 93.55% bc 97.67% abc

AVAUNT^®(300 ml/ha) 90.73% bc 89.02% c 95.22% bc

Control (Mean # of mines) 13 15 20

Countings on 20 haulms (leaves) /plot, 10 dd after each

treatments (# of mines and alive larvae)

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25.10.2013

Roveredo (VR) - ITALY 2013 (ITS-13-525)

Year : 2013 ‐ Cultivar : Vivaldi ‐ Contractor: AGREA 3 Applications: 3.07/15.07/26.07.2013

Observations: 12.08 (harvest)/5.09.2013 (after 23 days storage)

Remark: in this trial‐type, all cultural practices favouring PTM attacks on tubers are intentionally implemented.

TREATMENT COMPONENT

RATE

g a.s./ha UNIT TIMING

Experimental product 100.0 GAH

1ST APPLIC WHEN ADULTS TRAPPED, THEN COVER FLIGHT

STEWARD (WG 30.00 PC) + 37.5 GAH

CODACIDE (EC 95.00 PC) 2.5 L LMH

CORAGEN (SC 200.00 G/L) 35.0 GAH

UNTREATED CHECK NA UNTREATED TIMING (FP)

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25.10.2013

Roveredo (VR) - ITALY 2013 (ITS-13-525) Year : 2013 ‐ Cultivar : Vivaldi ‐ Contractor: AGREA 3 Applications: 3.07/15.07/26.07.2013

Observations: 12.08 (harvest)/5.09.2013 (after 23 days storage)

8/12/2013 9/5/2013

: :

I PHTOOP I PHTOOP

LARVA LARVA

P - SOLTU P - SOLTU

VIVALDI VIVALDI

DMP % DMP %

1.00 PL 1.00 PL TUS DMRT

5% TUS DMRT 5%

OPT.TRT#

Experimental 04 0.3 b 8.3 b-e

Steward+Codacide 07 0.0 b 5.0 cde

Coragen 08 0.5 ab 3.8 e

Untreated 999 1.3 a 15.0 a

0.788 5.32

NS **

0.382 2.58

149.56 42.62 LSD (0.05)

SIGNIFICANCE OF F STANDARD DEVIATION

COEFFICIENT OF VARIANCE STAGE HOST SPECIE TYPE / CODE HOST VARIETY SYMPTOM / METHOD EVALUATION BASIS / UNIT EVALUATION PART EVALUATION DATE EVALUATION TIME SPECIES TYPE / CODE VARIETY

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25.10.2013

Pegola di Malalbergo (BO) - ITALY 2013 (ITA-13-525) Year : 2013 ‐ Cultivar : Natasha ‐ Contractor: AGRI 2000 4 Applications: 19.07/30.07/9.08/21.08/2013

Observations: Oct. 2/2013 (after separate storage)

Remark: in this trial, no significant leaf damage was assessed.

TREATMENT COMPONENT

RATE

g a.s./ha UNIT TIMING

Experimental product 100.0 GAH

STEWARD (WG 30.00 PC) + 37.5 GAH

CODACIDE (EC 95.00 PC) 2.5 L LMH

CORAGEN (SC 200.00 G/L) 35.0 GAH

UNTREATED CHECK NA UNTREATED TIMING (FP)

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25.10.2013

2013 (ITA-13-525)- Pegola di Malalbergo (BO)

0 2 4 6 8 10 12 14

25‐giu 5‐lug 15‐lug 25‐lug 4‐ago 14‐ago 24‐ago 3‐set

PTM Flight Curve

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Trial results at harvest

Pegola di Malalbergo (BO) - ITALY 2013 (ITA-13-525)

% Infested Tubers Marktetable Yield (Tons/ha)

Rating Date Oct‐2‐2013 Oct‐2‐2013

Rating Type DMP% YIELD

Rating Unit % T‐MET

Sample Size, Unit 100 TUBER 2 ROW

Collection Basis, Unit 1 PLOT 1 PLOT

Days After First/Last Applic. 75 42 75 42

Trt‐Eval Interval 42 DA‐D 42 DA‐D

Days After Emergence 130 DE‐1 130 DE‐1

Trt Treatment Other Appl

No. Name Rate Rate Code 4 6

4

Experimental

Product 100 0,5 ABCD 10,5 c 40,60 ab

7

STEWARD 37,5 125 ABCD 9,0 c 38,75 b

CODACIDE 2370 2,5 ABCD

8

CORAGEN 35 0,175 ABCD 0,5 d 44,48 a

9

Untreated

Check 79,8 a 7,00 d

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25.10.2013

Pics from the tuber assessment (Sep. 5/2013)

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25.10.2013

Pics from the tuber assessment (Sep. 5/2013)

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Adults

Adult (direct and indirect effects)

Ovi-larvicidal (neonate die after hatching) Larvicidal (contact and ingestion)

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Coragen® 20 SC (Clorantraniliprole 200 g/L) residual effects on

Phthorimaea operculella Zeller adults

Nicola Mori ¹ , Andrea Bassi ²

1

AGREA Contract Research Organization

2

DU PONT DE NEMOURS ITALIANA

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Experimental design

Complete randomized (5 replications)

Plot size

1 potted potato plant

1 cage (approx. 20 x 20 x 60 cm) was assembled.

Application Handle sprayer

Water Volume Simulated 600 L/ha Statistical

analysis ANOVA + Tukey’s test Data

trasformation ^y ^{= x}⁺⁰^.⁰⁵ ^y⁼^arcsen ^x^/¹⁰⁰

Coragen® 20SC residual effects on P. operculella adults:

TRIAL DESIGN AND APPLICATION

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25.10.2013

Aim of this study (2010-2011) with Agrea & the Padua Univ. was to evaluate the efficacy

component on adults, also in order to better explain the observed “population reduction effects”

observed consequent to Rynaxypyr ^® applications.

First focus was on adults as they play a key role in the tuber infestation and many eggs can be laid in places (e.g. soil) where sprayate cannot reach

them.

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Coragen® 20SC residual effects on P. operculella adults:

RESULTS

Hours of exposure to the dried spray residue

Adult mortality

Regression Adult mortality/Hours of exposure to the dried spray residue.

F.P. rate 17.5-25.0 mL/hL

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Coragen® 20SC residual effects on P. operculella adults:

RESULTS

Hours of exposure to the dried spray residue

Nb. of eggs/leaf/female

Regression Eggs/Hours of exposure to the dried spray residue.

F.P. rate 17.5-25.0 mL/hL

y = -0,0049x + 0,4218 R² = 0,3372

ns

0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80

0 20 40 60 80 100

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Overall conclusions

The Lab. trials on “population reduction” effects indicate :

- adult mortality (25 to > 60%)

- very high and consistent reduction of egg-laying (up to 100%).

Both are positively correlated to the duration of the adult tarsal exposure (in the range 24-96 hours) and to the tested concentration (in the range 17.5 to 30 mL/hL).

* All the concs adopted in the lab. studies were on the low side vs. the real

use-concentration (in the range 30-60 mL/hL).

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25.10.2013

Normally the sprayate cannot wet well the tubers portions and soil particles in the

ground.

Once an egg is laid there, the animal is safe

& sound.

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25.10.2013

The ability to develop as a leafminer

The ability to develop in the tuber

Adult (population reduction)

Ovi-larvicidal (neonate die after hatching)

Larvicidal (contact & ingestion)

(35)

Effects on egg-hatching and larvae

Ovi-larvicidal (neonate die during/after hatching) Larvicidal (contact and ingestion)

(36)

Ovi-larvicidal activity

( Laboratory evaluations of the Potency and the Biological activity on T. absoluta).

36

Cages used for persistency tests at field-rate

Laboratory evaluations of the potency and the biological activity of chlorantraniliprole on T. absoluta.

Summary of performance as regards the insect stage DEMETER (NAGREF), ERDC, AGREA/UNIVERSITY OF PADUA 2010-2012).

Larvicidal activity for up to 21 days (aged-residue)

(37)

A new activity profile

Fast-acting Long-residual

Temperature-indipendant Rainfast

Adult

(population reduction) Ovi-larvicidal (neonate

die after hatching) Larvicidal (contact &

ingestion)

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25.10.2013

Andrea Bassi, Bologna, Oct. 22-2013 Rynaxypyr & Indoxacarb FD Coordinator, EMEA