Miele e Propoli

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Miele e Propoli per la salute umana

Dr. Simona Martinotti, Ph.D.

Dr. Elia Ranzato, Ph.D.

Department of Scienze e Innovazione Tecnologica – DISIT University of Piemonte Orientale Alessandria - Italy

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Alessandria

Vercelli

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Honey

• Clericuzio et al., J Nat Med 2014

• Clericuzio et al.,Planta Medica 2012

Jojoba oil

Ranzato et al., J Ethnopham 2011

• Ranzato et al., WRR 2012

• Ranzato et al., Burns & Trauma 2013 Ophioglossum vulgatum

Bruno et al.,Bioinorg Med Chem 2013

Dibenzofuran derivatives with antibacterial and wound-healing activity.

Patent number WO 2013/189950, December 2013.

Lichens

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Line of dressings containing Leptospermum Honey

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Wound closure?

Collagen production?

Matrix remodelling?

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EC₀₅% v/v EC₅₀% v/v

buckwheat ^0.47

(0.37-0.60)

1.22 (1.11-1.35)

manuka _0.35

(0.17-0.68)

2.18 (1.76-2.70)

acacia ^2.28

(1.69-3.08)

4.00 (3.59-4.46)

EC₀₅% v/v EC₅₀% v/v

buckwheat _1.49

(0.99-2.25)

4.07 (3.61-4.59)

manuka _2.07

(1.48-2.90)

4.74 (4.43-5.18)

acacia _2.04

(1.53-2.73)

4.83 (4.43-5.18) KERATINOCYTES

FIBROBLASTS

Ranzato et al., WWR 2012

Ranzato et al., Burns and Trauma 2013

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keratinocytes

a

b

c c

fibroblasts d

Ranzato et al., WWR 2012

Ranzato et al., Burns and Trauma 2013 a

b

d

c c

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Tight Juntions Occludin-1 Tight juntion protein ZO-1

Claudin-1

Adherens Juntions

E-cadherin

Vimentin Keratin-19

collagens

MMP-3 / MMP-9 Ranzato et al., WWR 2012

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Ranzato et al., BJD 2008

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Ranzato et al., 2012

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Inflammatory phase:

ANTISEPTIC ACTION

Re-epithelialization phase:

1) CYTOSKELETAL RE-ORGANIZATIONS 2) CELL MOTILITY INCREASE

3) EMT INDUCTION

4) INTRACELLULAR CALCIUM MODULATION

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When used topically to treat wounds or burns, honey is generally applied either directly in a thin coat, or in the form of honey-soaked dressing.

Topically applied drugs are commonly recovered at concentrations of about 1÷10 g Kg^-1 in skin tissue, while the levels of main bioactive compounds, like polyphenols, in honey range from about 50÷500 mg Kg^-1.

Hence, the direct application of 0.1% (1g Kg^-1) honey to cells in our experiments resulted in a delivery of about 0.05÷0.5 mg Kg^-1 bioactive compounds, suggesting that the effects that we have observed should be quite

achievable by topical honey application

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0 2000 4000 6000 8000 10000 12000

0.01% 0.1% 0.5% 0.01% 0.1% 0.5% 0.01% 0.1% 0.5%

manuka grano saraceno acacia

Assorbanza

con cellule

senza cellule