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UNIVERSITÀ DEGLI STUDI DI ROMA "TOR VERGATA"

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UNIVERSITÀ DEGLI STUDI DI ROMA

"TOR VERGATA"

FACOLTA' DI MEDICINA E CHIRURGIA

DOTTORATO DI RICERCA IN

NEUROSCIENZE

XX° CICLO

Titolo della tesi

ESPRESSIONE E LOCALIZZAZIONE SUBCELLULARE DELLA

ZINC FINGER PROTEIN 9, PRODOTTO DEL GENE DELLA

DISTROFIA MIOTONICA TIPO 2

Maria Beatrice Panico

Docente Guida:

Prof. R. Massa

Prof. Coordinatore: Prof. A. Pisani

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INDICE

INTRODUZIONE pag. 1

MATERIALI E METODI pag. 9

Animali e preparazione anticorpo pag. 9

Elettroforesi su gel e Western Blot pag. 10

Immunocitochimica pag. 11

Immunoelettromicroscopia pag. 14

RISULTATI pag. 15

Western Blot pag. 15

Immunocitochimica pag. 16

Immunoelettromicroscopia pag. 19

DISCUSSIONE pag. 20

BIBLIOGRAFIA pag. 25

Riferimenti

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