1
2
3
4
5
0
6
12
18
24
Ang-1 mRNA (fold increase)
Control siRNA
NF-kB siRNA
hour
Supplementary Figure 3. SIE cells were transfected with a fluorescein-conjugated control
siRNA or a NF-kB p65 siRNA (a pool of four oligonucleotides from Santa Cruz Biotechnology). After 18 hours, cell cultures were treated for 0, 10, and 18 hours with 50 ng/mL rDrm and the steady-state levels of Ang-1 mRNA were measured by quantitative RT-PCR analysis using an iCycler system with a SYBR Green master mix (Bio-Rad) according to manufacturer's instructions.