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g Analysis, by RT-qPCR, of Nova2 mRNA expression levels (relative to Ubb) and, by RT-PCR, of Unc5b exon 8 splicing in freshly puri fied ECs from mouse lung and colon tissue.. h

2 Quantitative RT-PCR analysis for TGFβ, IL-1β, CXCR2, CD86, COL I, αSMA, S100A4, CXCR4, and CD11a gene expression in cultures of systemic sclerosis (SSc) and healthy subject

From 16 chronic patients, seven cultures out of 138 (5%) were found to be positive using RT-PCR and then negative after cultures; from 29 intermittent patients, 18 out of 59

Confluent HSC were incubated for 48 hours in SFIF medium and then treated with different doses of canrenone 5 minutes before stimulation with PDGF-BB (10 ng/mL) for 10 minutes..

(a) Alizarin red-stained human MSC cultures treated with TB (containing 100 ng/mL BMP-2), FSC cocktails m4T5 or m8T11, or conventional osteogenic media (Conv-OM: containing 10 nM or

mauritanica eyes, we per- formed a semi-quantitative RT-PCR analysis and observed a strong expression of the SWS1 opsin gene in geckos eyes (Fig.. Yet, SWS1 mRNA was also observed

15,16 Similar results were available for microbiological diagnosis in spontaneous bac- terial peritonitis where RT-PCR increased the efficacy of cultures: RT-PCR detected

Total RNA (70 ng for each sample included in qRT-PCR-array profiling and 150 ng for qRT- PCR) was retrotranscribed to cDNA with RT 2 First Strand kit (Qiagen, Hilden, Germany)