- + + +
rDrm
- - + -
anti-Ang-1 Ab (0.1 ng/ml)
- - - +
anti-Ang-1 Ab (1.0 ng/ml)
p-Tyr
Tie-2
Supplementary Figure 1. Conditioned media from rDrm-treated SIE cells were added for
10 minutes to naïve serum-starved SIE cells in the absence or in the presence of neutralizing anti-Ang-1 antibodies. Then, immunoprecipitation with anti-Tie-2 antibodies was performed on the cell extracts followed by Western blotting with anti-phosphotyrosine antibodies (Santa Cruz). Uniform loading of the gel was confirmed by incubation of the membrane with anti-Tie-2 antibodies.
