I
Appendix 1
CM Sepharose chromatogram
Figure 15 R: Chromatogram profile of CM sepharose (1: loading, 2: washing, 3: elution)
II SP Sepharose
Figure 16R: Chromatogram profile of SP sepharose (1: loading, 2: washing, 3: elution)
III MEP Hypercell
Figure 17R: Chromatogram profile of MEP hypercel (1: loading, 2: washing, 3: washing, 4: elution)
IV MBI Hypercell
Figure 18R: Chromatogram profile of MBI hypercel with Supernatant of Fraction I (1: loading, 2: washing, 3: elution, 4: sanitization)
V MBI Hypercell
Fig 19R: Chromatogram profile of MBI hypercel with Fraction II+III (1: loading, 2: washing, 3: elution, 4: sanitization)
VI Expanded bed adsorption
Fig 20R: Chromatogram profile of EBA with Fraction II+III (1: loading, 2: washing, 3: elution, 4: sanitization)
VII MBI on radial column
Fig 21R: Chromatogram profile of MBI hypercel with Supernatant of Fraction I on radial column (1: loading, 2: washing, 3: elution, 4: sanitization)
VIII Hyper D Q
Fig 22R: Chromatogram profile of HyperD Q first run and second run with Supernatant of Fraction I
IX Capto S
Figure 23R: Chromatogram profile of Capto S preliminary results with Supernatant of Fraction I (1: loading, 2: washing, 3: elution, )
X TMAE Fractogel
Figure 24R: Chromatogram profile of TMAE Fractogel preliminary results with Supernatant of Fraction I (1: loading, 2: washing, 3: elution, )
XI Ig select
Fig 25R: Chromatogram profile of IgG select with Supernatant of Fraction I (1: loading, 2: washing, 3: elution, )
XII CaptoS
Figure 26R: Chromatogram profile of Capto S scale up trials with Supernatant of Fraction I (1: loading, 2: washing, 3: elution, )
1 2
XIII TMAE Fractogel
Figure 27R: Chromatogram profile of TMAE Fractogelscale up trials with Supernatant of Fraction I (1: loading, 2: washing, 3: elution, 4 sanitisation)
1 2