Appendix 1 CM Sepharose chromatogram Figure 15 R: Chromatogram profile of CM sepharose (1: loading, 2: washing, 3: elution)

I

Appendix 1

CM Sepharose chromatogram

Figure 15 R: Chromatogram profile of CM sepharose (1: loading, 2: washing, 3: elution)

II SP Sepharose

Figure 16R: Chromatogram profile of SP sepharose (1: loading, 2: washing, 3: elution)

III MEP Hypercell

Figure 17R: Chromatogram profile of MEP hypercel (1: loading, 2: washing, 3: washing, 4: elution)

IV MBI Hypercell

Figure 18R: Chromatogram profile of MBI hypercel with Supernatant of Fraction I (1: loading, 2: washing, 3: elution, 4: sanitization)

V MBI Hypercell

Fig 19R: Chromatogram profile of MBI hypercel with Fraction II+III (1: loading, 2: washing, 3: elution, 4: sanitization)

VI Expanded bed adsorption

Fig 20R: Chromatogram profile of EBA with Fraction II+III (1: loading, 2: washing, 3: elution, 4: sanitization)

VII MBI on radial column

Fig 21R: Chromatogram profile of MBI hypercel with Supernatant of Fraction I on radial column (1: loading, 2: washing, 3: elution, 4: sanitization)

VIII Hyper D Q

Fig 22R: Chromatogram profile of HyperD Q first run and second run with Supernatant of Fraction I

IX Capto S

Figure 23R: Chromatogram profile of Capto S preliminary results with Supernatant of Fraction I (1: loading, 2: washing, 3: elution, )

X TMAE Fractogel

Figure 24R: Chromatogram profile of TMAE Fractogel preliminary results with Supernatant of Fraction I (1: loading, 2: washing, 3: elution, )

XI Ig select

Fig 25R: Chromatogram profile of IgG select with Supernatant of Fraction I (1: loading, 2: washing, 3: elution, )

XII CaptoS

Figure 26R: Chromatogram profile of Capto S scale up trials with Supernatant of Fraction I (1: loading, 2: washing, 3: elution, )

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XIII TMAE Fractogel

Figure 27R: Chromatogram profile of TMAE Fractogelscale up trials with Supernatant of Fraction I (1: loading, 2: washing, 3: elution, 4 sanitisation)

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