Second-Cycle Degree Course in REPRODUCTIVE BIOTECHNOLOGIES

(1)

Second-Cycle Degree Course in REPRODUCTIVE BIOTECHNOLOGIES

A.Y. 2018-2019

(2)

1.

EDUCATIONAL AND

PROFESSIONAL GOALS 2.

TEACHINGS’

BALANCE

3. RESEARCH AREAS

(3)

Cell biology and physiology of gametogenesis, fertilization

and embryo development

Gamete handling

Embryo culture

Andrology

Micromanipulation techniques (IVM, IVF, ICSI) Advances in

cryobiology Epigenetic, toxicology,

angiogenesis concepts related to reproduction Pre-implantation

genetic diagnosis

Ethical and legal controversies surrounding assisted

reproduction in humans

Students will receive training in :

1. EDUCATIONAL GOALS

The Course will provide the fundamental theoretical and practical knowledge necessary to understand and perform up-to-date techniques in the areas of Assisted Reproduction Technology (ART).

The MSc is a laboratory-based

science degree and is taught by

world-leading academics and

leading reproductive medicine

specialists from professional world.

(4)

MAIN CAREER OPPORTUNITIES FOR GRADUATES IN RP

ACADEMIA

II LEVEL MASTER

PhD COURSE

EMPLOYMENT

CLINICS OF ASSISTED REPRODUCTIVE

TECHNOLOGY

LIVESTOCK CENTERS

DIAGNOSTIC/

RESEARCH CENTERS

COMMERCIAL AREA

UNITE

Cellular and Molecular Biotechnology

Second-cycle Degree Course in REPRODUCTIVE BIOTECHNOLOGIES

The Reproductive Biotechnologies MSc allows graduates to pursue a career in assisted reproduction and/or research in the reproductive sciences, both in human and animal field.

1. PROFESSIONAL GOALS

(5)

EMBRYOLOGISTS or BIOTECHNOLOGISTS (73%)

RESEARCHERS or PhD STUDENTS (27%)

LONDON (UK) LEUVEN (Holland) MURCIA (Spain) CACERES (Spain) PHILADELPHIA (USA)

WIEN (AUSTRIA)

TERAMO ++

CHIETI LONDON WIESBADEN

WARSAW CAMBRIDGE

MILANO +++

BOLOGNA MODENA REGGIO EMILIA ASCOLI PICENO TERAMO ++

L’AQUILA PESCARA ROMA++

NAPOLI SALERNO CASERTA BARI POTENZA

A sample of 33 graduates in RP from UniTE (from Linkedin)

18/33 6/33

LONDON (UK) ++

CAMBRIDGE (UK) ++

WIESBADEN (Germany) WARSAW (Poland)

9/33

Second-cycle Degree Course in REPRODUCTIVE BIOTECHNOLOGIES

EDUCATIONAL RESULTS

(6)

I YEAR

Structure and Function of the Reproductive System Biology of gametes, IVM and IVF Techniques

Andrology I

Molecular Markers in Reproduction Embryology

Cryobiology

Scientific Communication, part I BASIC TEACHING SUBJECTS

(+++ animal samples)

II YEAR

Genetics of Human Reproduction

Diagnostic Histology Applied to Cells and Tissues of the RS Andrology II

ICSI Procedure and Advanced Techniques in PMA

Legislation and Bioethics in Medicine of Reproduction Scientific Communication, part II

PROFESSIONALISING TEACHING SUBJECTS (+++ human samples)

2. TEACHINGS’ BALANCE

ELECTIVE COURSES - Angiogenesis and reproduction

- Developmental and reproductive toxicology

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(7)

Career Days (stakeholders)

Preliminary meetings (all teachers)

Info requested to each teacher

Name:………..

E-mail:…………

Skype:………..

Phone:………….

Office:………….

Course Title:

Objectives:

Interactions:

Research Interest:

Second-cycle Degree Course in REPRODUCTIVE BIOTECHNOLOGIES

2. TEACHINGS’

BALANCE

(8)

EXAMPLES

(9)

BASIC KNOWLEDGE in:

 Cytology

 Cell Physiology and Cell Culture

 Biochemistry and Molecular Biology

 Applied and Medical Genetics

 English language

I YEAR

II YEAR

Elective courses ENTRY REQUIREMENTS

Bachelor or Master degree in an appropriate

biological-, biomedical- or veterinary- science subject, medicine or healthcare science (Biotechnologies, Life Sciences, Livestock science, Nursing, Pharmacy,

Medicine, Veterinary Medicine,…)

ENTRANCE TEST (not mandatory)

+ REMEDIAL

COURSES (Nov-Dec)

The course is delivered using: lectures,

seminars, team based learning exercises, flipped classrooms, presentations and problem-based- learning sessions or case studies, and self- directed learning.

Theoretical training is complemented by a consistent number of hands-on laboratory- based practical sessions.

The course content is enhanced by extensive online resources and the provision of all lessons through the E- learning platform.

Jan-June

Jan-May Nov

Graduation

(10)

3. RESEARCH AREAS

(11)

http://old.iss.it/rpma/index.php?lang=1&anno=2019&tipo=6

WHERE TO FIND USEFUL INFORMATION:

REGISTRO NAZIONALE PMA (in italian)

(12)

https://www.facebook.com/Reproductive-Biotechnologies- University-of-Teramo

http://www.unite.it/UniTE/Didattica/Corsi_di_laurea_2018_2019/

Programme Director:

Alessia Colosimo acolosimo@unite.it

Orientation

Educational Manager:

Natalia Izzi nizzi@unite.it

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(13)

Second-cycle Degree Course in REPRODUCTIVE BIOTECHNOLOGIES

FIRST YEAR

 STRUCTURE AND FUNCTION OF THE REPRODUCTIVE SYSTEM 11 Cfu

 BIOLOGY OF GAMETES, IVM AND IVF TECHNIQUES 10

 ANDROLOGY I 6

 MOLECULAR MARKERS IN REPRODUCTION 12

 EMBRYOLOGY 6

 CRYOBIOLOGY 11

 SCIENTIFIC COMMUNICATION, PART I 3

(14)

Name: Valentina Russo E-mail: vrusso@unite.it Skype: russoval

Phone: 0861 266930

Office: 3^rdfloor – Faculty of Bioscience

Course Title: STRUCTURE AND FUNCTION OF THE REPRODUCTIVE SYSTEM (I year) Objectives:

to provide an in-depth knowledge on the neuroendocrine mechanisms and on the

existing relations between the structure and function of the female reproductive system in domestic animals and humans

AMNIOTIC STEM CELL BIOLOGY, REGENERATIVE MEDICINE, TISSUE ENGINEERING

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(15)

Name: Barbara Barboni E-mail: bbarboni@unite.it Skype: barbara barboni Phone: 0861 266878

Course Title: BIOLOGY OF GAMETES, IVM AND IVF TECHNIQUES (I year):

- BIOLOGY OF GAMETES Objectives:

To provide deepened knowledge and methods on mechanisms &

signalling pathways involved in mammalian gametes differentiation, growth, maturation and activation.

FOLLICULOGENESIS IN VITRO, OOCYTE QUALITY, IVM INNOVATION

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(16)

Name: Luisa Gioia E-mail: lgioia@unite.it Skype: luisagioiaaq Phone: 0861 266857

Course Title: BIOLOGY OF GAMETES, IVM AND IVF TECHNIQUES (I year):

- TECHNIQUES OF IN VITRO MATURATION AND FERTILIZATION Objectives:

to train students to develop theoretical and practical skills on techniques of in vitro maturation (IVM) and in vitro fertilization (IVF) using spermatozoa and oocytes of domestic animals

REGULATION OF OOCYTE IVM; MARKERS OF OOCYTE QUALITY; MOLECULAR

MECHANISMS INVOLVED IN GAMETE INTERACTION AND IVF; MECHANISMS OF CELL DEATH IN GAMETES AND FOLLICULAR CELLS

MII

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(17)

Name: Alberto Contri E-mail: acontri@unite.it Phone: 0861 266995 Mobile: 3479131616

Office: I^st floor – Faculty of Veterinary Medicine Loc. Piano d’Accio

Course Title: ANDROLOGY I (I year) Objectives:

the course provides information about the normal structure and function of animal sperm. The student will be trained on the basic and advanced

sperm evaluation in mammals.

DETERMINANTS OF SPERM PRESERVATION IN THE BULL AND DOG; SPERM MATURATION IN STALLION AND TOM; IMPROVEMENT OF HYGENIC AND FUNCTIONAL QUALITY OF PRESERVED SPERM IN BULL, DOG, AND STALLION

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(18)

Name: Natalia Battista E-mail: nbattista@unite.it Skype: nataliabattista Phone: 0861 266844

Office: 3^rdFloor – Faculty of Bioscience

Course Title: MOLECULAR MARKERS IN REPRODUCTION (I year)

- TECHNIQUES FOR BIOMARKERS IN HUMAN REPRODUCTION Objectives:

To achieve the knowledge of the main concepts concerning theoretical and practical methodologies that enable the identification and quantification of various

biological biomarkers of fertility.

BIOCHEMISTRY OF BIOACTIVE LIPIDS INVOLVED IN

INFLAMMATORY, NEURODEGENERATIVE AND REPRODUCTIVE PROCESSES

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(19)

Course Title: MOLECULAR MARKERS IN REPRODUCTION (I year) - BIOMARKERS IN HUMAN REPRODUCTION

Objectives:

to provide students with a core of skills on the biochemical nature of the different classes of compounds that act as intra- and inter-cellular messengers in the molecular and cellular mechanisms of the reproductive processes.

BIOCHEMISTRY OF BIOACTIVE AND NUTRACEUTICAL LIPIDS IN THE MOLECULAR MECHANISMS OF

PHYSIOLOGICAL PROCESSES, INCLUDING REPRODUCTIVE EVENTS AND INFLAMMATORY, NEURODEGENERATIVE AND TUMOR PATHOLOGIES

Name: Cinzia Rapino E-mail: crapino@unite.it Phone: 0861 266842

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(20)

Name: Pasqualino Loi, DVM, PhD E-mail: ploi@unite.it

Phone: 0861 266856

Course Title: EMBRYOLOGY (I year) Objectives:

To provide students with an updated view of basic embryology in mammals and other model organisms (Fish, Amphybians, Birds, Insects). To get students acquainted with the classical

micromanipulation techniques used for assisted reproduction in animals and humans as well as in experimental embryology.

NUCLEAR REPROGRAMMING-REMODELLING, SOMATIC CELL NUCLEAR TRANSFER, MONOPARENTAL EMBRYOS, EMBRYO-MATERNAL INTERACTION, BIODIVERSITY PRESERVATION

In vitro nuclear remodelling Loi, et al.

Nuclear Transfer, Loi, et al.,

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(21)

Course Title: CRYOBIOLOGY (I year)

- MOLECULAR INTERACTIONS AMONG GAMETES AND ENVIRONMENT Objectives:

The course aims at providing an updated description of the molecular interactions and mechanisms responsible for a functional modulation of gametes and embryo after cryopreservation. A particular focus will be on the protein/protein and

protein/membrane interactions, ROS production, and epigenetic modifications.

Research interests:

MEMBRANE BIOLOGY AND FUNCTIONAL INTERACTIONS AMONG BIOACTIVE LIPIDS AND MEMBRANE ENZYMES AND RECEPTORS IN CELL MODELS AND GAMETES

Name: Enrico Dainese E-mail: edainese@unite.it Skype: enrico_dainese Phone: 0861/266876

Office: 3^rdFloor–Faculty of Bioscience

From: Dainese et al., Biochem. J. (2014) 457, 463–472

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(22)

Name: Nicola Bernabò E-mail: nbernabo@unite.it Skype: nicolabernabo Phone: 0861 266836

Course Title: CRYOBIOLOGY (I Year)

- CRYOPRESERVATION TECHNIQUES OF GAMETES Objectives:

To give students an updated information about the criopreservation techniques of gametes and embryos.

SIGNAL TRANSDUCTION OF SPERMATOZOA; CAPACITATION; IVF; SYSTEMS AND

COMPUTATIONAL BIOLOGY ^F^ro

m: Bernabòet a.,Front Physiol.2017 Dec22;8:1097.

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(23)

Second-cycle Degree Course in REPRODUCTIVE BIOTECHNOLOGIES

SECOND YEAR

 GENETICS OF HUMAN REPRODUCTION 10 Cfu

 DIAGNOSTIC HISTOLOGY APPLIED TO CELLS AND TISSUES OF THE RS 5

 ANDROLOGY II 6

 ICSI PROCEDURE AND ADVANCED TECHNIQUES IN PMA 6

 LEGISLATION AND BIOETHICS IN MEDICINE OF REPRODUCTION 6

 SCIENTIFIC COMMUNICATION, PART II 3

(24)

Name: Alessia Colosimo E-mail: acolosimo@unite.it Phone: 0861 266840

Course Title: GENETICS OF HUMAN REPRODUCTION (II year) Objectives:

the course provides information on infertility-related diseases due to genetic causes.

The student will be trained on the basic and advanced molecular tests, PGD/PGS tests and counseling protocols for infertile couples.

AMNIOTIC STEM CELLS IN REGENERATIVE MEDICINE; ADVANCED MOLECULAR AND

CYTOGENETICAL TECHNIQUES IN DIAGNOSIS OF INFERTILITY (Un. CHIETI); EPIGENETIC AND NUTTIGENETIC FACTORS INVOLVED IN MALE INFERTILITY (Un. CHIETI)

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(25)

Name: Leonardo Della Salda E-mail: ldellasalda@unite.it Phone: 0861 266866

Office: OVUD–Faculty of Veterinary Medicine

Course Title: DIAGNOSTIC HISTOLOGY APPLIED TO CELLS AND TISSUES OF THE REPRODUCTIVE SYSTEM (II Year)

Objectives:

To provide understanding of the pathogenesis and morphological aspects of cell injury/death, cellular growth and neoplasia of the reproductive system in domestic animals and humans

SIGNAL TRANSDUCTION PATHWAYS IN REPRODUCTIVE NEOPLASIA, CANCER /MESENCHIMAL INTERACTION

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(26)

Name: Arcangelo Barbonetti (University of L’Aquila) E-mail: arcangelobarbonetti@virgilio.it

Phone: 347 6413629

Office: University of L’Aquila

Course Title: ANDROLOGY II(II Year) Objectives:

To reproduce validated protocols commonly applied to laboratory diagnosis of male infertility, in sperm processing for assisted fertilization procedures and in basic/clinical research in the field of reproductive medicine.

HUMAN SPERM BIOLOGY; SPERM OXIDATIVE DAMAGE; MALE INFERTILITY;

REPRODUCTION ENDOCRINOLOGY; HYPOGONADISM

ROS

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(27)

Name: Ilaria Listorti E-mail: ilistorti@unite.it Phone: 06 8550385 Office: Alma Res - Rome

Course Title: ICSI PROCEDURE AND ADVANCED TECHNIQUES IN MEDICALLY- ASSISTED PROCREATION (II year)

Objectives:

To show the importance of biological knowledge when performing diagnosis, prevention and treatment of human infertility

IVF; EMBRYO CULTURE; OXIDATIVE STRESS AND INFERTILITY

ICSI

Embryo culture

Blastocyst biopsy

Preimplantation Genetic Test

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(28)

Course Title:SCIENTIFIC COMMUNICATION(I and II year) Objectives:

To give knowledge and understanding of cultural, social and political issues around science communication; to learn how to use scientific communication through printed paper and online (internet) news (I year) and how to use scientific communication on television and radio (II year).

MEDICAL SPEAKING AND WRITING ABILITY, INFORMATION RETRIEVAL, SCIENTIFIC

READING AND WRITING, LISTENING AND OBSERVING, INFORMATION REPRESENTATION AND KNOWLEDGE PRESENTATION, SOCIAL NETWORKS IN MEDICAL PRACTICE.

Name: Massimo Cecaro

E-mail: massimo@cecaro.com

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(29)

Course Title: LEGISLATION AND BIOETHICS IN MEDICINE OF REPRODUCTION (II Year)

Objectives:

To provide a complete and updated description of legislation and bioethics in medicine of reproduction, comparing the Italian legislative body to the ones of different European countries.

WOMEN'S RIGHTS IN ITALY, WITH REGARD TO ACCESS TO WORK Name: Angela Musumeci

E-mail: amusumeci@unite.it Phone: 0861 266629

Office: 3^rdfloor – Faculty of Law

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(30)

Second-cycle Degree Course in REPRODUCTIVE BIOTECHNOLOGIES

ELECTIVE COURSES

 ANGIOGENESIS AND REPRODUCTION 3 Cfu

 DEVELOPMENTAL AND REPRODUCTIVE TOXICOLOGY 5

(31)

Name: Alessandra Martelli E-mail: amartelli@unite.it Phone: 0861 266889

Course: ANGIOGENESIS AND REPRODUCTION (Elective course) Objectives:

To provide an in-depth understanding ovarian chronological angiogenesis modulation during ovarian folliculogenesis: from the primordial follicles to the antral periovulatory phases

MORPHOLOGICAL ASPECTS AND MOLECULAR AND MORPHO-FUNTIONAL MODIFICATIONS

INVOLVED IN THE RECOGNITION OF THE MALE AND FEMALE GAMETES AND DURING FOLLICULAR GROWTH

Second-cycle Degree Course in

REPRODUCTIVE BIOTECHNOLOGIES

(32)

Name: Monia Perugini E-mail: mperugini@unite.it Skype: monia.perugini Phone: 0861 266988

Course Title: DEVELOPMENTAL AND REPRODUCTIVE TOXICOLOGY (Elective course) Objectives:

To describe the mechanisms leading to infertility or alteration during the embryo

development and to give an overview of chemicals associated to the reproductive problems in humans and animals.

TOXICOLOGICAL TESTS, EMBRYONIC ZEBRAFISH MODEL, MOLECULAR PATHWAYS, DEVELOPMENTAL TOXICITY