Gilson, Inc. World Headquarters I 3000 Parmenter Street I P.O. Box 620027 Middleton, WI 53562-0027 USA
Tel: (1) 800-445-7661 or (1) 608-836-1551 I Fax: (1) 608-831-4451
Gilson S.A.S. I 19, avenue des Entrepreneurs LT800511/C © 05/2012 Gilson SAS.
w w w.gilson.com I sales@gilson.com I ser vice@gilson.com I training@gilson.com
Premium pipetting comfort!
EXTREMELY LOW PIPETTING FORCEs!
EXTREMELY EAsY TIP EJECTION!
LEGENdARY PIPETMAN RObusTNEss!
Ad just Able-Vol ume Pi Pet te
Trusted results
Best results guaranteed with a performance exceeding ISO recommendations.
Model Volume tested
Maximum Permissible Errors
Gilson ISO 8655
Systematic error (μL)
Random error (μL)
Systematic error (μL)
Random error (μL) P2G
(F144054P) (F144054M)
Min.: 0.2 0.5 Max.: 2
± 0.024
± 0.025
± 0.030
≤ 0.012
≤ 0.012
≤ 0.014
± 0.08
± 0.08
± 0.08
≤ 0.04
≤ 0.04
≤ 0.04 P10G
(F144055P) (F144055M)
Min.: 1
5 Max.: 10
± 0.025
± 0.075
± 0.100
≤ 0.012
≤ 0.030
≤ 0.040
± 0.12
± 0.12
± 0.12
≤ 0.08
≤ 0.08
≤ 0.08 P20G
(F144056P) (F144056M)
Min.: 2
10 Max.: 20
± 0.10
± 0.10
± 0.20
≤ 0.030
≤ 0.050
≤ 0.060
± 0.2
± 0.2
± 0.2
≤ 0.1
≤ 0.1
≤ 0.1 P100G
(F144057P) (F144057M)
Min.: 10
50 Max.: 100
± 0.35
± 0.40
± 0.80
≤ 0.10
≤ 0.12
≤ 0.15
± 0.8
± 0.8
± 0.8
≤ 0.3
≤ 0.3
≤ 0.3 P200G
(F144058P) (F144058M)
Min.: 20
100
Max.: 200
± 0.50
± 0.80
± 1.60
≤ 0.20
≤ 0.25
≤ 0.30
± 1.6
± 1.6
± 1.6
≤ 0.6
≤ 0.6
≤ 0.6 P1000G
(F144059P) (F144059M)
Min.: 100 500 Max.: 1000
± 3.0
± 4.0
± 8.0
≤ 0.6
≤ 1.0
≤ 1.5
± 8.0
± 8.0
± 8.0
≤ 3.0
≤ 3.0
≤ 3.0 P5000G
(F144066) Min.: 500
2500 Max.: 5000
± 12
± 15
± 30
≤ 3
≤ 5
≤ 8
± 40
± 40
± 40
≤ 15
≤ 15
≤ 15 P10mL G
(F144067)
Min.: 1000 5000 Max.: 10000
± 30
± 40
± 60
≤ 6
≤ 10
≤ 16
± 60
± 60
± 60
≤ 30
≤ 30
≤ 30
Gilson’s Complete Pipetting System
Your protocols and experiments require more than just generic pipette tips. Indeed, tips are essential components of your pipetting system. According to ISO 8655-2*:
“The maximum permissible errors always apply to the total system of piston pipette and tip. When using pipette tips not delivered by the pipette supplier, the supplier declaration or certificate of conformity does not apply”.
*ISO 8655 is an international standard for “piston operated apparatus”. It defines, among other things, terms and definitions regarding piston operated pipettes and their testing, testing procedure and maximum permissible errors.
Pipette model Recommended PIPETMAN DIAMOND tips
Model (scale 1/2) Volume range
P2G
D10 0.1 – 10 µL
DL10 0.1 – 20 µL
DF10 0.1 – 10 µL
DFL10 0.1 – 10 µL
P10G
D10 0.1 – 10 µL
DL10 0.1 – 20 µL
DF10 0.1 – 10 µL
DFL10 0.1 – 10 µL
P20G D200 2 – 200 µL
DF30 2 – 30 µL
P100G D200 2 – 200 µL
DF100 20 – 100 µL
P200G
D200 2 – 200 µL
D300 20 – 300 µL
DF200 20 – 200 µL
DF300 20 – 300 µL
P1000G
D1000 100 – 1000 µL
D1200 100 – 1200 µL
DF1000 100 – 1000 µL
DF1200 100 – 1200 µL
P5000 G D5000 1 – 5 mL
P10mL G D10mL 1 – 10 mL
PIPETMAN G kits complement the product range and offer cost-effective package to get you started or to renew your old set of pipette.
Pipette Kits
All you need to get started, in a single box!
PIPETMAN G Ordering reference Pipette Models* PIPETMAN DIAMOND Tips STARTER KIT
F167900 P20G - P200G - P1000G D200 - D1000
MICRO-VOLUME KITF167800 P2G - P10G - P100G DFL10ST - DF100ST
*With both ejectors: stainless steel and plastic ejectors.
POS. 1 066803
POS. 2 066805 POS. 3 066807
POS. 4 066811
POS. 5 066813
Premium pipetting comfort!
EXTREMELY LOW PIPETTING FORCEs!
EXTREMELY EAsY TIP EJECTION!
LEGENdARY PIPETMAN RObusTNEss!
PIPETMAN G is a new mechanical, air-displacement pipette from Gilson.
PIPETMAN G offers you the lowest pipetting force on the market thanks to a piston assembly system that gives you ideal pipetting and purge forces sensations.
Enjoy a new addition to PIPETMAN line with its legendary accuracy, precision, and robustness… with the highest comfort!
TAKE COMfORT TO ThE NExT LEVEL
KEEP yOUR TEChNIQUE CONSISTENT
Use PIPETMAN G like the PIPETMAN Classic!
You can trust in your results and benefit from a great pipetting experience. Don’t change your habits, even the ratio between pipetting and purge forces is maintained!
Complete your equipment with:
Good Laboratory Practice (GLP) features ensure the high quality and reliability of your work.
CARROUSELTM pipette holder (Ref. F161401)
SINGLETM pipette holder (Ref. F161406)
TRACKMANTM (Ref. F70301)
Model Ordering reference
Volume Range Color code Plastic ejector stainless steel ejector
P2G F144054P F144054M 0.2 µL to 2 µL Orange
P10G F144055P F144055M 0.5 µL to 10 µL Red
P20G F144056P F144056M 2 µL to 20 µL Pale Yellow
P100G F144057P F144057M 10 µL to 100 µL Salmon
P200G F144058P F144058M 20 µL to 200 µL Yellow
P1000G F144059P F144059M 100 µL to 1000 µL Blue
Model Ordering reference Volume Range Color code
P5000G* F144066 500 µL to 5000 µL Purple
P10mL G* F144067 1 mL to 10 mL Light Blue
Vial of lubricant** 5440011070 For all models of PIPETMAN G
PIPETMAN G provides the legendary robustness and trustable accuracy in results of the PIPETMAN Classic.
It perfectly fulfils your expectations in terms of pipetting comfort, even for the most intensive pipetting routine.
Thanks to the redesigned piston assembly (new seal, lighter springs) and the addition of high quality pis- ton grease, you can enjoy one of the lowest* pipetting force available on the market!
GET A COMPLETE VOLUME RANGE
Get yourself a complete range of PIPETMAN G pipettes and benefit from the same comfort, robustness and confidence in result from 2µL to 10mL!
ChOOSE yOUR LOOK:
CLASSIC OR MODERN DESIGN
Stainless steel or plastic ejectors are available to you. They both have the legendary PIPETMAN robustness and are equipped with a clip ejector for a convenient access to the serviceable parts.
When working with corrosive liquid we recommend using the plastic ejector.
TRIOTM pipette holder (Ref. F161405
EASy IDENTIfICATION
To easily distinguish your new PIPETMAN G from your PIPETMAN Neo or Classic pipette, a blue ring has been placed under the thumbwheel.
Blue ring PIPETMAN G =
Stainless steel ejector Plastic ejector
Clip eje cto
r
*P5000G and P10mL G models do not require tip ejectors.
**PIPETMAN G can only be lubricated with a high quality lubricant selected and approved by Gilson. The use of other types of lubricants will void the warranty for your pipette.
0,0 Competitor A Volume Setting Force (N.cm)Newton
PIPETMAN Neo Competitor B Model (200 or 300 µL)
Competitor C PIPETMAN M 1,0
2,0 3,0 4,0 5,0
0
PIPETMAN G Competitor A - Pipette
1
Competitor B - Pipette 1
Competitor A - Pipette 2
Competitor C - Pipette 1
Competitor A - Pipette 4 Competitor D - Pipette
1
Competitor A - Pipette 3 1
3 4 5 6
2 7 8 9 10 11
Competitor E - Pipette 1
PIPETMAN Neo Competitor B - Pipette
2
Competitor E - Pipette 2 Competitor
F
Competitor E - Pipette 3
Competitor B - Pipette 3
Competitor C - Pipette 2 Competitor A - Pipette 5
Competitor D - Pipette 2
Competitor C - Pipette 3
* Tested on Gilson and other brands of 200 µL pipette or equivalent.
POS. 1 066803 POS. 2 066805 POS. 3 066807
POS. 5 066813
POS. 4 066811
Caratteristiche tecniche pipette Gilson Pipetman® G Pipette manuali a spostamento d’aria, a volume variabile.
• Gamma di volumi da 0.2 µl a 10 ml
• Forze di pipettaggio molto fluide grazie al nuovo pistone assemblato con molle e guarnizioni più morbide.
• Indicatore di volume analogico a tre cifre.
• Regolazione in continuo del volume.
• L’espulsore può essere in plastica o in acciaio inox. L’espulsore in plastica è consigliato quando si usano liquidi corrosivi e molto aggressivi.
• Numero di serie impresso a caldo sul corpo pipetta, dal quale si può risalire al mese e all’anno di fabbricazione.
• Progettate, prodotte, calibrate e validate in conformità con le specifiche e i metodi dello standard ISO8655.
• Corredate di certificato di conformità originale Gilson.
• Conformi alla Normativa IVD (98/79/EC) per gli strumenti In – Vitro Diagnostici.
• Garanzia 12 mesi.
Codici e specifiche tecniche
Modello Codice
Volume (µL)
Errori massimi tollerabili (µL) Gilson ISO 8655 Errore Errore Errore Errore Sistematico Casuale Sistematico Casuale P2G
F144054P F144054M
Min. 0.2 0.5
Max. 2
± 0.024 ≤ 0.012 ± 0.025 ≤ 0.012 ± 0.030 ≤ 0.014 ± 0.08 ≤ 0.04 ± 0.08 ≤ 0.04 ± 0.08 ≤ 0.04 P10G F144055P F144055M Min. 1
5
Max. 10
± 0.025 ≤ 0.012 ± 0.075 ≤ 0.030 ± 0.100 ≤ 0.040 ± 0.12 ≤ 0.08 ± 0.12 ≤ 0.08 ± 0.12 ≤ 0.08 P20G F144056P F144056M Min. 2
10
Max. 20
± 0.10 ≤ 0.03 ± 0.10 ≤ 0.05 ± 0.20 ≤ 0.06 ± 0.20 ≤ 0.10 ± 0.20 ≤ 0.10 ± 0.20 ≤ 0.10 P100G F144057P F144057M Min. 10
50
Max. 100
± 0.35 ≤ 0.10 ± 0.40 ≤ 0.12 ± 0.80 ≤ 0.15 ± 0.80 ≤ 0.30 ± 0.80 ≤ 0.30 ± 0.80 ≤ 0.30 P200G F144058P F144058M Min. 20
100
Max. 200
± 0.50 ≤ 0.20 ± 0.80 ≤ 0.25 ± 1.60 ≤ 0.30 ± 1.60 ≤ 0.60 ± 1.60 ≤ 0.60 ± 1.60 ≤ 0.60 P1000G F144059P F144059M Min. 100
500
Max. 1000
± 3 ≤ 0.6 ± 4 ≤ 1.0 ± 8 ≤ 1.5 ± 8 ≤ 3.0 ± 8 ≤ 3.0 ± 8 ≤ 3.0 P5000G F144066 Min. 500
2500
Max. 5000
± 12 ≤ 3
± 15 ≤ 5
± 30 ≤ 8
± 40 ≤ 15
± 40 ≤ 15
± 40 ≤ 15
P10mlG F144067 Min. 1000
5000
Max. 10000
± 30 ≤ 6
± 40 ≤ 10
± 60 ≤ 16
± 60 ≤ 30
± 60 ≤ 30
± 60 ≤ 30
- Con una buona tecnica di pipettaggio la P2G può essere usata a 0.1 µL e la P10G a 0.5 µL.
- Nel codice la lettera P indica l’espulsore in plastica, mentre la lettera M l’espuslore in metallo.
- La P5000G e la P10ml non hanno l’espulsore.
POS. 1 066803 POS. 2 066805
POS. 3 066807
POS. 5 066813
POS. 4 066811
Premium Pipetting Comfort!
IMPORTANT NOTICE!
This pipette can be only lubricated with a high quality lubricant selected and approved by Gilson. This lubricant is available under the reference: 5440011070.
The use of other type of lubricant cancels the warranty of this pipette.
1 - IntroductIon
PIPETMAN G is a fully adjustable air displacement pipette and is used with disposable tips. To answer the current needs of our loyal users of ours pipettes, and still being conform to Gilson brand qualities, three improvements have been added to our mechanical series:
pipetting and purge forces dramatically decreased*,
large volumes, 5 mL and 10 mL very comfortable,
you have the choice for an ejector plastic or a stainless steel ejector.
* Implementation of new features to improve dramatically pipetting comfort has no impact on the legendary robustness, accuracy and precision of PIPetman.
Eight single channel models cover a volume range from 0.2 µL to 10 mL.
Contents Page
1 - INTRODUCTION 2
2 - Pa RTs CheCk LIsT 3
3 - DesCRIPTION 3
4 - seTTING The VOLUMe 4
5 - PIPeTTING 4
6 - GeNeRa L GUIDeLINes f OR GOOD PIPeTTING 7
7 - aCCess ORIes 8
8 - GLP fea TURes 9
9 - TROUbLesh OOTING 9
10 - Leak TesT 11
11 - Ma INTeNa NCe 12
12 - CLea NING a ND DeCONTaMINaTION 15
13 - sPeCIf ICaTIONs 18
14 - sPa Re Pa RTs 20
eC DeCLa RaTION Of CONf ORMITY 22
Wa RRa NTY 23
Decreased pipetting forces are due to the new de- signed piston assembly including the use of a very high quality of lubricant. The use of other lubricant cancels the warranty of this pipette. This lubricant is available under the reference: 5440011070.
Please contact your Gilson distributor.
2 - Parts check LIst
Just take a moment to verify that the following items are present:
PIPETMAN G, User's Guide, Safety bag,
Certificate of conformity (including bar-code sticker).
3 - descrIPtIon
Please refer to the following chapters for a full description of the different parts and functions of the pipette.
PIPeTMa N DIaMOND® tip Tip-ejector Tip-holder handle Tip-ejector button Color-coded push-button
Thumbwheel
Connecting nut blue ring
4 - settIng the VoLume
The volume of liquid to be aspi- rated is set using the volumeter.The dials are colored either black or red to indicate the position of the decimal point, depending on the model (see examples).
The volume is set by turn- ing the thumbwheel or the push-button. The push-button makes it easier and quicker to set volumes, especially when wearing gloves. The thumb- wheel may be turned using only one hand to slowly reach the required setting.
To obtain maximum accuracy when setting the volume, proceed as follows:
when decreasing the volume setting, slowly reach the required setting, making sure not to overshoot the mark.
when increasing the volume setting, pass the required value by 1/3 of a turn and then slowly decrease to reach the volume, making sure not to overshoot the mark.
5 - PIPettIng
For optimum performance, use of PIPETMAN DIAMOND Tips with your PIPETMAN G is strongly recommended.
These tips, made from pure polypropylene have the Model Color of volumeter numbers
Black Red Increment P2G µL 0.01 µL 0.002 µL P10G to P20G µL 0.1 µL 0.02 µL P100G-P200G µL - 0.2 µL P1000G 0.01 mL mL 0.002 mL P5000G 0.01 mL mL 0.002 mL P10mlG mL 0.01 mL 0.02 mL
Push-button Thumbwheeel 1
5 P2G
1.25 µL
2 0
75 P10G
7.5 µL 12 5 P20G
12.5 µL P100G
0 75 75 µL
P200G 1 25 125 µL
P1000G 0 75
0.75 mL P5000G
12 5 1.25 mL
P10mlG 07 5 7.5 mL
Gilson logo engraved on their collar, ensuring that you have a genuine Gilson product. Plastic tips are for a single application – they must not be cleaned for reuse.
PIPetman G can also be used with the main tip brands.
Fitting the tips
To fit a new PIPETMAN DIAMOND Tip, push the tip-holder into the tip using a slight twisting motion to ensure a firm, airtight seal.
For the P2G and P10G models equipped with stainless steel tip-ejector,
A dual-position adapter (plastic) is required to fit DL10 tips (long tips) or D10 tips (short tips).
P2G and P10G models are delivered with the adapter in place, ready to use DL10 tips. If D10 tips are used, the adapter must be repositioned in the shorter slot as follows:
Pull the adapter down from the metallic rod.
Turn the adapter through 180°C.
Refit the adapter so that the end of the metallic rod engages the shorter slot of the adapter.
For the P2G and P10G models equipped with plastic tip-ejector,
A tip-ejector extension is supplied to fit with D10 tips (short tips).
To fit a tip-ejector extension:
Slide the extension over the tip-holder.
Push the extension firmly onto the end of the tip-ejector until it clicks into place.
To remove a tip-ejector extension:
Gently twist the extension.
Pull it away from the pipette.
Short tips Long tips
Pre-rinse the tips
Some liquids (e.g. protein-containing solutions and organic solvents) can leave a film of liquid on the inside wall of the tip ; pre-rinse the tip to minimize any errors that may be related to this phenomenon.
Pre-rinsing consists of aspirating the first volume of liquid and then dispensing it back into the same vessel (or to waste). Subsequent volumes that you pipette will have levels of accuracy and precision within specifications.
aspirate
Press the push-button to the first stop (this corresponds to the set volume of liquid).
Hold the pipette vertically and immerse the tip in the liquid (see immersion depth table, page 10). Release the push-button slowly and smoothly (to top posi- tion) to aspirate the set volume of liquid. Wait one second (time depends on model, see table) ; then withdraw the pipette-tip from the liquid. You may wipe any droplets away from the outside of the tip using a medical wipe, however if you do so take care to avoid touching the tip’s orifice.
First stop Second stop
dispense
Place the end of the tip against the inside wall of the recipient vessel (at an angle of 10° to 40°).
Press the push-button slowly and smoothly to the first stop.
Wait for at least a second, then press the push-button to the second stop to expel any residual liquid from the tip. Keep the push-button pressed fully down and (while removing the pipette) draw the tip along the inside surface of the vessel.
Release the push-button, smoothly. Eject the tip by pressing firmly on the tip-ejector button.
6 - generaL guIdeLInes For good PIPettIng
Make sure that you operatethe push-button slowly and smoothly.
When aspirating, keep the tip at a constant depth below the surface of the liquid (refer to the table).
Change the tip before as- pirating a different liquid, sample, or reagent.
Change the tip if a droplet remains at the end of the tip from the previous pipetting operation.
Each new tip should be pre-rinsed with the liquid to be pipetted.
Liquid should never enter the tip-holder; to prevent this:
- press and release the push-button slowly and smoothly,
- never turn the pipette upside down,
- never lay the pipette on its side when there is liquid in the tip.
If you use the same tip with a higher volume, pre- Table - Immersion Depth and Wait Time
Model Immersion Wait Time Depth (mm) (seconds)
P2G 1 1
P10G 1 1
P20G 2-3 1
P100G 2-4 1
P200G 2-4 1
P1000G 2-4 2-3
P5000G 3-6 4-5
P10mlG 5-7 4-5
For volatile solvents you should saturate the air- cushion of your pipette by aspirating and dispensing the solvent repeatedly before aspirating the sample.
When pipetting liquids with temperatures different to the ambient temperature, pre-rinse the tip several times before use.
You may remove the tip-ejector (see Chapter 11 - Maintenance) to aspirate from very narrow tubes.
After pipetting acids or other corrosive liquids that emit vapors, remove the tip-ejector, the tip-holder, rinse, dry and lubricate the piston (see Chapter 11 - Maintenance). For the model P1000G, by using a specific tip holder equipped with a filter, you can increase the lifetime of the piston (see Chapter 7 - Accessories).
Do not pipette liquids having temperatures above 70 °C or below 4 °C. The pipette can be used between + 4 °C and + 40 °C but the specifications may vary according to the temperature (refer to the ISO 8655-2 standard for conditions of use).
7 - accessorIes
To make pipetting more comfortable and more secure, Gilson has developed several accessories:
To avoid the possibility of liquid running back into the pipette, store the pipette vertically.
To identify or personalize your pipette, COLORIS™
clips are available:
COLORIs™ clips (mixed colors set of 10) f 161301 COLORIs™ clips (red, set of 10) f 161302 COLORIs™ clips (yellow, set of 10) f 161303 COLORIs™ clips (green, set of 10) f 161304 COLORIs™ clips (blue, set of 10) f 161305 Ca RROUseL™ Pipette stand (7 pipettes) f 161401
TRIO™ stand (3 pipettes) f 161405
sINGLe™ pipette holder f 161406
With THE JIMMY™, hands free microtube opener, you can open both snap-cap and screw-cap microtubes.
To protect the piston when pipetting corrosive liquids, you can use a specific tip holder and filter for the model P1000G:
8 - gLP Features
The Serial Number is en- graved on the body of the pipette. It provides unique identification of your pipette and the date of manufacture.Ex : AA10369
The Bar Code on the box and the certificate of conformity provide traceability of your pipette.
9 - troubLeshootIng
A quick inspection of the pipette may help you to detect a problem.
You may download from the Gilson website (www.gilson.com) the “2 minute inspection”, which shows how to perform a quick diagnosis of your pipette.
The JIMMY™ (set of 3) f 144983
Corrosion protection kit (tip holder + a bag of 10 filters) f 144570
Before returning any pipette to your local Gilson Service Center, ensure that it is completely free of chemical, biological, or radioactive contamination. Refer to Chapter 12 - Cleaning and Decontamination. Please use the included safety bag to return the pipette to your local Gilson Service Center.
The following table may help you to identify and correct the problem you might encounter.
Symptom Possible Cause Action Pipette is Damaged tip-holder Replace the tip-holder leaking sample Worn O-ring or seal Replace both parts and lubricate Pipette won’t Worn O-ring Replace both parts and lubricate aspirate Damaged tip-holder Replace the tip-holder
Connecting nut is loose Tighten connecting nut Damaged or corroded piston Return pipette to supplier Improper repair or assembly see Chapter 11 - Maintenance Pipette is Improper repair or assembly see Chapter 11 - Maintenance inaccurate Unscrewed tip-holder Tighten connecting nut
Connecting nut is loose Tighten connecting nut Pipette is Tip-holder is loose Tighten connecting nut not precise Connecting nut is loose Tighten connecting nut
Incorrect operator technique Operator training Damaged or corroded piston(s) Return pipette to supplier Damaged tip-holder(s) Replace the tip-holder Worn O-ring or seal Replace both parts and lubricate Tips fall off or Low quality tips Use PIPeTMaN DIaMOND tips do not fit Dirty tip-holder Clean the tip-holder with alcohol correctly Damaged tip-holder(s) Replace the tip-holder
Damaged tip-ejector Replace tip-ejector
ejector spacer is missing Mount the spacer on the tip- ejector The ejector spacer is damaged Replace the ejector spacer The tip-ejector is loose assemble the tip-ejector properly The ejector lock is misaligned align the ejector lock Pipetting seize up Piston need lubricant Lubricate piston assembly However, if you can’t solve the problem, contact your Gilson distributor.
10 - Leak test
This test may be performed at any time to check that the pipette does not leak, especially after performing a main- tenance or decontamination procedure. If a pipette fails this test, replace the O-ring and seal. After making sure that the pipette is correctly reassembled, repeat this test.
For the P2g to P200g models:
Fit a PIPETMAN DIAMOND Tip.
Set the pipette to the maximum volume given in the specifications, and pre-rinse.
Aspirate the set volume from a beaker of distilled water.
Maintain the pipette in the vertical position and wait for 20 seconds.
If a water droplet appears at the end of the tip there is a leak.
If you see no droplet, re-immerse the tip below the surface of water.
The water level inside the tip should remain constant;
if the level goes down there is a leak.
For the P1000g, P5000g and P10mlg models:
Fit a PIPETMAN DIAMOND tip.
Set the pipette to the maximum volume given in the specifications.
Aspirate the set volume from a beaker of distilled water.
Maintain the pipette in the vertical position and wait for 20 seconds.
If a water droplet appears at the end of the tip, there is a leak.
11 - maIntenance
Routine maintenance will help keep your pipette in good condition, ensuring a continued high level of performance.
Maintenance is limited to:
Cleaning or decontamination (see Chapter 12 - Cleaning and Decontamination)
Replacing spare parts
Greasing the piston assembly.
PIPeTmaN P2G and P10G should not be disassembled, so you may only replace the push-button, tip-ejector, dual position tip-ejector and its adapter. With these pipettes if the tip-holder is damaged, the piston may also be damaged.
After replacing any parts you should verify the performance of your pipette following the verification procedure available on the Gilson website (www.gilson.com). If the pipette needs to be readjusted, please contact your local Gilson authorized Service Center.
changing the tip-ejector To remove
Push the ejection button.
Push laterally the tip-ejector.
Slide and remove the tip-ejector.
To refit
Push the ejection button.
Slide the tip-ejector along the tip-holder.
Clip the tip-ejector on the body of the pipette.
P10G, P20G P100G, P200G
P5000G P10mlG P1000G
Tip-holder
Tip-ejector Connecting nut Color coded push-button
Piston assembly
Blue ring
changing the tip-holder – no tools required Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it counter- clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly and the seals.
Clean, autoclave, or replace the tip-holder.
If necessary, lubricate lightly the piston and its seals (see below).
Reassemble the pipette (refer to the figure, p 16).
Tighten the connecting nut (turn clockwise).
Refit the tip-ejector (see above).
servicing the Piston assembly
You may remove the piston assembly for cleaning pur- poses only. If the piston assembly is changed, the pipette must be adjusted and calibrated in a Gilson authorized Service Center. As the models P2G and P10G contain miniaturized parts, it is best not to disassemble these pipettes yourself.
The piston assembly must not be autoclaved.
Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it counter-clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly and the seals.
Leave exposed the piston, clean it with isopropanol or ethanol and lubricate lightly. For P100G, P200G and P1000G models, lu- bricate only the useful part of the piston and the seals. For P5000G and P10mlG, lubricate the tip of the piston, disassemble the seals
The use of other lubricant cancels the warranty of this pipette.
Reassemble the pipette (refer to the figure, page 13).
Tighten the connecting nut (turn clockwise).
Refit the tip-ejector (see above).
changing the seals
The O-ring and seal are on the piston; they must not be autoclaved, if worn or damaged in any way (chemical or mechanical), they must be replaced. As the models P2G and P10G contain miniaturized parts, it is best not to disassemble these pipettes yourself, please contact your local Gilson authorized Service Center.
The dimensions of the O-ring vary depending on the model of pipette.
Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it counter- clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly, O-ring and seal.
If necessary clean the piston and replace the seal;
lubricate them lightly. Please place them in the correct order.
Reassemble the pipette (refer to the figure, page 13).
Tighten the connecting nut (turn clockwise).
Refit the tip-ejector (see above).
12 - cLeanIng and decontamInatIon
PIPETMAN G is designed so that the parts normally in contact with liquid contaminants, can easily be cleaned and decontaminated. However, because the models P2G and P10G contain miniaturized parts, it is best not to disassemble these pipettes yourself; please contact your local Gilson authorized Service Center.You may refer to the decontamination procedure available on the Gilson website (www. gilson.com).
Liquid must never enter the upper part (handle) of any pipette.
cleaning
The pipette must be cleaned, as described below, before it is decontaminated. Soap solution is recommended for cleaning PIPETMAN G.
External
Remove the tip-ejector.
Wipe the tip-ejector with a soft-cloth or lint-free tissue impregnated with soap solution.
Wipe the entire pipette with a soft-cloth or lint- free tissue impregnated with soap solution, to remove all dirty marks. If the pipette is very dirty, a brush with soft plastic bristles may be used.
Wipe the entire pipette and the tip-ejector with a soft cloth or lint-free tissue soaked with distilled water.
Refit the tip-ejector and allow the pipette to dry.
Internal
The following components only can be immersed in a cleaning solution: connecting nut, tip-ejector, tip-holder, piston assembly, seal and O-ring.
Disassemble the pipette as described in the Chapter 11 - Maintenance.
Set aside the upper part in a clean, dry place.
Clean the individual components of the lower part of the pipette using an ultrasonic bath (20 minutes at 50°C) or with a soft-cloth and brushes. Note that the piston assembly and seals must be degreased with isopropanol or ethanol before being immersed in the ultra sonic bath. Small round brushes with soft plastic bristles may be used to clean the interior of the tip-holder.
Rinse the individual components with distilled water.
Leave the parts to dry by evaporation or wipe them with a clean soft-cloth or lint-free tissue.
Reassemble the pipette as described in the Chapter 11 - Maintenance.
autoclaving
The upper part (body) and the piston assembly of the pipette are not autoclavable. Only the following parts may be autoclaved: tip-ejector, tip-holder and connecting nut. The O-ring and seal are not autoclavable; they may be cleaned or replaced with the one specified in Chapter 14 - Spare Parts.
Clean the parts to be autoclaved, especially the tip-holder.
Put the parts in an autoclaving sack.
Autoclave for 20 minutes at 121°C, 0.1 MPa.
Check that the parts are dry before re-assembling the pipette.
Set the pipette aside to stabilize at room temperature.
chemical decontamination
You may choose to decontaminate your pipette chemi- cally, in accordance with your own procedures. Whatever decontaminant you use, check with the supplier of the decontaminant that it is compatible with stainless steel and the plastics used in the construction of the pipette:
PA (Polyamide), PBT (Polybutylene Terephtalate), PC (Polycarbonate), PC/PBT (Polycarbonate/ Polybutylene Terephtalate), POM (Polyoxymethylene), or PVDF (Poly- vinylidene Fluoride).
Upper Part (handle)
Wipe the upper part (handle) of the pipette with a soft-cloth or lint-free tissue impregnated with the chosen decontaminant.
Wipe the upper part of the pipette with a soft- cloth or lint-free tissue soaked with distilled water or sterile water.
Lower Part (Volumetric module)
The following components only can be immersed in a
Piston assembly and seals must be degreased with isopropanol or ethanol before being immersed in de- contamination solution in separate vessel.
Remove the tip-ejector, the tip-holder and the con- necting nut.
Immerse them in the cleaning solution.
Degrease the piston assembly, the seals and immerse them in another vessel.
Rinse each component with distilled water.
Leave the parts to dry by evaporation (or wipe with a soft cloth the tip-ejector, the tip-holder and con- necting nut).
Lubricate the piston assembly and the seals.
Reassemble the piston assembly, the tip-holder and the tip-ejector.
13 - sPecIFIcatIons
PIPETMAN G is a high quality pipette that offers excel- lent accuracy and precision. The figures given in the
“Gilson Maximum Permissible Errors” table (page 19) were obtained using PIPETMAN DIAMOND Tips. These figures are guaranteed only when genuine PIPETMAN DIAMOND Tips are used.
Each pipette is inspected and validated by qualified technicians in accordance with the Gilson Quality System.
Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 standard, by type testing.
The adjustment is carried out under strictly defined and monitored conditions (ISO 8655-6).
The data given in the tables conform to the ISO 8655-2 Standard.
With a precise pipetting technique (see Chapter 6 - General guidelines for good pipetting) the P2G model may be used to aspirate volumes as low as 0.1 µL and the P10G model as low as 0.5 µL.
gilson maximum Permissible errors
Maximum Permissible Errors
Model Volume Gilson ISO 8655
(Reference) (µL) systematic Random systematic Random
error (µL) error (µL) error (µL) error (µL)
P2G Min 0.2 ± 0.024 0.012 ± 0.08 0.04
(f144054P) 0.5 ± 0.025 0.012 ± 0.08 0.04
(f144054M) Max. 2 ± 0.030 0.014 ± 0.08 0.04 P10G Min. 1 ± 0.025 0.012 ± 0.12 0.08
(f144055P) 5 ± 0.075 0.030 ± 0.12 0.08
(f144055M) Max 10 ± 0.100 0.040 ± 0.12 0.08 P20G Min. 2 ± 0.10 0.03 ± 0.20 0.10
(f144056P) 10 ± 0.10 0.05 ± 0.20 0.10
(f144056M) Max. 20 ± 0.20 0.06 ± 0.20 0.10
P100G Min. 10 ± 0.35 0.10 ± 0.80 0.30
(f144057P) 50 ± 0.40 0.12 ± 0.80 0.30
(f144057M) Max. 100 ± 0.80 0.15 ± 0.80 0.30 P200G Min. 20 ± 0.50 0.20 ± 1.60 0.60
(f144058P) 100 ± 0.80 0.25 ± 1.60 0.60
(f144058M) Max. 200 ± 1.60 0.30 ± 1.60 0.60 P1000G Min. 100 ± 3 0.6 ± 8 3.0
(f144059P) 500 ± 4 1.0 ± 8 3.0
(f144059M) Max. 1000 ± 8 1.5 ± 8 3.0
P5000G Min. 500 ± 12 3 ± 40 15
(f144066) 2500 ± 15 5 ± 40 15
Max. 5000 ± 30 8 ± 40 15
P10mlG Min. 1000 ± 30 6 ± 60 30
(f144067) 5000 ± 40 10 ± 60 30
Max. 10000 ± 60 16 ± 60 30
each pipette model (except P5000G and P10mlG) has two different ordering references to identify the kind of tip-ejector required. For a pipette with a plastic tip-ejector, the ordering reference is ended by the letter P, for a pipette with a stainless steel tip-ejector, the ordering reference is ended by the letter m.
ex: For a P10G model with the plastic tip-ejector the ordering reference is F144055P. For the same pipette with a stainless steel tip-ejector, the ordering reference is F044055m.
Service Kit 1st level includes:
- 3 piston seals or seal guides
- 3 O-rings - 1 tip-holder
Service Kit 2nd level includes:
- 1 push-button - 1 connecting nut - 2 tip-ejectors
for the P2G and P10G models only:
- 1 adapter for stainless steel tip-ejector
- 1 extension for plastic tip-ejector
a
b
c
F4
F2
F3 a
b c
d e
F3
F4
14 - sPare Parts
F2 F1
d
e F1
F1 F2
P20g (F144056 P or m) and P100g (F144057 P or m)
Description P20G P100G
C+D+E Service Kit 1st level FA07002 FA07003
A+B+F Service Kit 2nd level F1619722 F1619732
C+D Seal guide + O-ring (5 sets) FA07013 FA07014
P200g (F144058 P or m) and P1000g (F144059 P or m)
Description P200G P1000G
C+D+E Service Kit 1st level FA07004 FA07005
A+B+F Service Kit 2nd level F1619742 F1619752
C+D Seal guide + O-ring (5 sets) FA07015 FA07016
P2g (F144054P or m) and P10g (F144055P or m)
Description P2G P10G
C+D+E Service Kit 1st level F144501 FA07001
A+B+F1 to F4 Service Kit 2nd level F1619702 F1619712 C+D Seal + O-ring (5 sets) F144861 FA07012 F3 Tip-ejector adapter F144879 F144879 F4 Tip-ejector extension F2070903 F2070903
P5000g (F144066) and P10mlg (F144067)
Description P5000G P10mlG
C+D+E Service Kit 1st level FA07021 FA07022
A Push-button assembly FA07019 FA07020 C+D Seal + O-ring (5 sets) FA07017 FA07018
E Tip-holder F123608 F161263
each pipette model (except P5000G and P10mlG) has two different ordering references to identify the kind of tip-ejector required. For a pipette with a plastic tip-ejector, the ordering reference is ended by the letter P, for a pipette with a stainless steel tip-ejector, the ordering reference is ended by the letter m.
ex: For a P10G model with the plastic tip-ejector the ordering reference is F144055P. For the same pipette with a stainless steel tip-ejector, the ordering reference is F044055m.
aLL modeLs
Description Ordering reference
ec decLaratIon oF conFormIty
PIPetman® g
P2G, P10G, P20G, P100G, P200G, P1000G, P5000G and P10mlG
98/79/ec*
on In Vitro Diagnostic Medical Devices comply with the requirements of the following European Directives:
Villiers-le-Bel, May 3rd, 2012
a. el sayed h. Le dorze
General Manager Quality Manager
gILson s.a.s.
19, avenue des Entrepreneurs BP. 145 - 95400 Villiers-le-Bel (France) Tel.: +33(0) 1 34 29 50 00
Fax: +33(0) 1 34 29 50 20 www.gilson.com
* annex III, self-declared The company,
Hereby certifies on its sole responsibility that the products listed below:
Warranty
Gilson warrants this pipette against defects in material under normal use and service for a period of 12 months from the date of purchase.
This warranty shall not apply to pipettes which are subject to abnormal use and/or improper or inadequate maintenance (contrary to the recommendations given in the User’s guide), including, but not limited to pipettes which have been subjected to physical damage, improper handling, or spillage or exposure to any corrosive environment. This warranty shall also be void in the event pipettes are altered or modified by any party other than Gilson or its designates. Gilson’s sole liability under this warranty shall be limited to, at Gilson’s sole option, repair or replacement of any defective components of pipettes or refund of the purchase price paid for such pipettes.
the ForegoIng Warranty Is eXcLusIVe and gILson hereby dIscLaIms aLL other WarrantIes, Whether eXPress or ImPLIed, IncLudIng any WarrantIes oF merchantabILIty and any WarrantIes oF FItness For a PartIcuLar PurPose, under no cIrcumstances shaLL gILson be LIabLe For any conseQuentIaL, PunItIVe, IndIrect or IncIdentaL damages arIsIng out oF any breach oF any eXPress or ImPLIed Warranty.
LT801122/B - ©2012 Gilson SAS All rights reserved May 2012
English Printed in France
Specifications subject to change without notifications - errors omitted.
gilson, Inc. World headquarters
3000 Parmenter Street I P.O. Box 620027 I Middleton, WI 53562-0027, USA Tel: (1) 800-445-7661 or (1) 608-836-1551 I Fax: (1) 608-831-4451 gilson s.a.s.
19, avenue des Entrepreneurs I BP 145, F-95400 Villiers-le-Bel, France Tel: +33 (0) 1 34 29 50 00 I Fax: +33 (0) 1 34 29 50 20
www.gilson.com
sales@gilson.com service@gilson.com training@gilson.com
Premium Pipetting Comfort!
IMPORTANT NOTICE!
This pipette can be only lubricated with a high quality lubricant selected and approved by Gilson. This lubricant is available under the reference: 5440011070.
The use of other type of lubricant cancels the warranty of this pipette.
1 - IntroductIon
PIPETMAN G is a fully adjustable air displacement pipette and is used with disposable tips. To answer the current needs of our loyal users of ours pipettes, and still being conform to Gilson brand qualities, three improvements have been added to our mechanical series:
pipetting and purge forces dramatically decreased*,
large volumes, 5 mL and 10 mL very comfortable,
you have the choice for an ejector plastic or a stainless steel ejector.
* Implementation of new features to improve dramatically pipetting comfort has no impact on the legendary robustness, accuracy and precision of PIPetman.
Eight single channel models cover a volume range from 0.2 µL to 10 mL.
Contents Page
1 - INTRODUCTION 2
2 - PaRTs CheCk LIsT 3
3 - DesCRIPTION 3
4 - seTTING The VOLUMe 4
5 - PIPeTTING 4
6 - GeNeRaL GUIDeLINes fOR GOOD PIPeTTING 7
7 - aCCessORIes 8
8 - GLP feaTURes 9
9 - TROUbLeshOOTING 9
10 - Leak TesT 11
11 - MaINTeNaNCe 12
12 - CLeaNING aND DeCONTaMINaTION 15
13 - sPeCIfICaTIONs 18
14 - sPaRe PaRTs 20
eC DeCLaRaTION Of CONfORMITY 22
Decreased pipetting forces are due to the new de- signed piston assembly including the use of a very high quality of lubricant. The use of other lubricant cancels the warranty of this pipette. This lubricant is available under the reference: 5440011070.
Please contact your Gilson distributor.
2 - Parts check LIst
Just take a moment to verify that the following items are present:
PIPETMAN G, User's Guide, Safety bag,
Certificate of conformity (including bar-code sticker).
3 - descrIPtIon
Please refer to the following chapters for a full description of the different parts and functions of the pipette.
PIPeTMaN DIaMOND® tip Tip-ejector Tip-holder handle Tip-ejector button Color-coded push-button
Thumbwheel
Connecting nut blue ring
4 - settIng the VoLume
The volume of liquid to be aspi- rated is set using the volumeter.The dials are colored either black or red to indicate the position of the decimal point, depending on the model (see examples).
The volume is set by turn- ing the thumbwheel or the push-button. The push-button makes it easier and quicker to set volumes, especially when wearing gloves. The thumb- wheel may be turned using only one hand to slowly reach the required setting.
To obtain maximum accuracy when setting the volume, proceed as follows:
when decreasing the volume setting, slowly reach the required setting, making sure not to overshoot the mark.
when increasing the volume setting, pass the required value by 1/3 of a turn and then slowly decrease to reach the volume, making sure not to overshoot the mark.
5 - PIPettIng
For optimum performance, use of PIPETMAN DIAMOND Tips with your PIPETMAN G is strongly recommended.
These tips, made from pure polypropylene have the Model Color of volumeter numbers
Black Red Increment P2G µL 0.01 µL 0.002 µL P10G to P20G µL 0.1 µL 0.02 µL P100G-P200G µL - 0.2 µL P1000G 0.01 mL mL 0.002 mL P5000G 0.01 mL mL 0.002 mL P10mlG mL 0.01 mL 0.02 mL
Push-button Thumbwheeel 1
5 P2G
1.25 µL
2 0
75 P10G
7.5 µL 12 5 P20G
12.5 µL P100G
0 75 75 µL
P200G 1 25 125 µL
P1000G 0 75
0.75 mL P5000G
12 5 1.25 mL
P10mlG 07 5 7.5 mL
Gilson logo engraved on their collar, ensuring that you have a genuine Gilson product. Plastic tips are for a single application – they must not be cleaned for reuse.
PIPetman G can also be used with the main tip brands.
Fitting the tips
To fit a new PIPETMAN DIAMOND Tip, push the tip-holder into the tip using a slight twisting motion to ensure a firm, airtight seal.
For the P2G and P10G models equipped with stainless steel tip-ejector,
A dual-position adapter (plastic) is required to fit DL10 tips (long tips) or D10 tips (short tips).
P2G and P10G models are delivered with the adapter in place, ready to use DL10 tips. If D10 tips are used, the adapter must be repositioned in the shorter slot as follows:
Pull the adapter down from the metallic rod.
Turn the adapter through 180°C.
Refit the adapter so that the end of the metallic rod engages the shorter slot of the adapter.
For the P2G and P10G models equipped with plastic tip-ejector,
A tip-ejector extension is supplied to fit with D10 tips (short tips).
To fit a tip-ejector extension:
Slide the extension over the tip-holder.
Push the extension firmly onto the end of the tip-ejector until it clicks into place.
To remove a tip-ejector extension:
Gently twist the extension.
Pull it away from the pipette.
Short tips Long tips
Pre-rinse the tips
Some liquids (e.g. protein-containing solutions and organic solvents) can leave a film of liquid on the inside wall of the tip ; pre-rinse the tip to minimize any errors that may be related to this phenomenon.
Pre-rinsing consists of aspirating the first volume of liquid and then dispensing it back into the same vessel (or to waste). Subsequent volumes that you pipette will have levels of accuracy and precision within specifications.
aspirate
Press the push-button to the first stop (this corresponds to the set volume of liquid).
Hold the pipette vertically and immerse the tip in the liquid (see immersion depth table, page 10). Release the push-button slowly and smoothly (to top posi- tion) to aspirate the set volume of liquid. Wait one second (time depends on model, see table) ; then withdraw the pipette-tip from the liquid. You may wipe any droplets away from the outside of the tip using a medical wipe, however if you do so take care to avoid touching the tip’s orifice.
First stop Second stop
dispense
Place the end of the tip against the inside wall of the recipient vessel (at an angle of 10° to 40°).
Press the push-button slowly and smoothly to the first stop.
Wait for at least a second, then press the push-button to the second stop to expel any residual liquid from the tip. Keep the push-button pressed fully down and (while removing the pipette) draw the tip along the inside surface of the vessel.
Release the push-button, smoothly. Eject the tip by pressing firmly on the tip-ejector button.
6 - generaL guIdeLInes For good PIPettIng
Make sure that you operatethe push-button slowly and smoothly.
When aspirating, keep the tip at a constant depth below the surface of the liquid (refer to the table).
Change the tip before as- pirating a different liquid, sample, or reagent.
Change the tip if a droplet remains at the end of the tip from the previous pipetting operation.
Each new tip should be pre-rinsed with the liquid to be pipetted.
Liquid should never enter the tip-holder; to prevent this:
- press and release the push-button slowly and smoothly,
- never turn the pipette upside down,
- never lay the pipette on its side when there is liquid in the tip.
If you use the same tip with a higher volume, pre- Table - Immersion Depth and Wait Time
Model Immersion Wait Time Depth (mm) (seconds)
P2G 1 1
P10G 1 1
P20G 2-3 1
P100G 2-4 1
P200G 2-4 1
P1000G 2-4 2-3
P5000G 3-6 4-5
P10mlG 5-7 4-5
For volatile solvents you should saturate the air- cushion of your pipette by aspirating and dispensing the solvent repeatedly before aspirating the sample.
When pipetting liquids with temperatures different to the ambient temperature, pre-rinse the tip several times before use.
You may remove the tip-ejector (see Chapter 11 - Maintenance) to aspirate from very narrow tubes.
After pipetting acids or other corrosive liquids that emit vapors, remove the tip-ejector, the tip-holder, rinse, dry and lubricate the piston (see Chapter 11 - Maintenance). For the model P1000G, by using a specific tip holder equipped with a filter, you can increase the lifetime of the piston (see Chapter 7 - Accessories).
Do not pipette liquids having temperatures above 70 °C or below 4 °C. The pipette can be used between + 4 °C and + 40 °C but the specifications may vary according to the temperature (refer to the ISO 8655-2 standard for conditions of use).
7 - accessorIes
To make pipetting more comfortable and more secure, Gilson has developed several accessories:
To avoid the possibility of liquid running back into the pipette, store the pipette vertically.
To identify or personalize your pipette, COLORIS™
clips are available:
COLORIs™ clips (mixed colors set of 10) f161301 COLORIs™ clips (red, set of 10) f161302 COLORIs™ clips (yellow, set of 10) f161303 COLORIs™ clips (green, set of 10) f161304 COLORIs™ clips (blue, set of 10) f161305 CaRROUseL™ Pipette stand (7 pipettes) f161401
TRIO™ stand (3 pipettes) f161405
sINGLe™ pipette holder f161406
With THE JIMMY™, hands free microtube opener, you can open both snap-cap and screw-cap microtubes.
To protect the piston when pipetting corrosive liquids, you can use a specific tip holder and filter for the model P1000G:
8 - gLP Features
The Serial Number is en- graved on the body of the pipette. It provides unique identification of your pipette and the date of manufacture.Ex : AA10369
The Bar Code on the box and the certificate of conformity provide traceability of your pipette.
9 - troubLeshootIng
A quick inspection of the pipette may help you to detect a problem.
You may download from the Gilson website (www.gilson.com) the “2 minute inspection”, which shows how to perform a quick diagnosis of your pipette.
The JIMMY™ (set of 3) f144983
Corrosion protection kit (tip holder + a bag of 10 filters) f144570
Before returning any pipette to your local Gilson Service Center, ensure that it is completely free of chemical, biological, or radioactive contamination. Refer to Chapter 12 - Cleaning and Decontamination. Please use the included safety bag to return the pipette to your local Gilson Service Center.
The following table may help you to identify and correct the problem you might encounter.
Symptom Possible Cause Action Pipette is Damaged tip-holder Replace the tip-holder leaking sample Worn O-ring or seal Replace both parts and lubricate Pipette won’t Worn O-ring Replace both parts and lubricate aspirate Damaged tip-holder Replace the tip-holder
Connecting nut is loose Tighten connecting nut Damaged or corroded piston Return pipette to supplier Improper repair or assembly see Chapter 11 - Maintenance Pipette is Improper repair or assembly see Chapter 11 - Maintenance inaccurate Unscrewed tip-holder Tighten connecting nut
Connecting nut is loose Tighten connecting nut Pipette is Tip-holder is loose Tighten connecting nut not precise Connecting nut is loose Tighten connecting nut
Incorrect operator technique Operator training Damaged or corroded piston(s) Return pipette to supplier Damaged tip-holder(s) Replace the tip-holder Worn O-ring or seal Replace both parts and lubricate Tips fall off or Low quality tips Use PIPeTMaN DIaMOND tips do not fit Dirty tip-holder Clean the tip-holder with alcohol correctly Damaged tip-holder(s) Replace the tip-holder
Damaged tip-ejector Replace tip-ejector
ejector spacer is missing Mount the spacer on the tip- ejector The ejector spacer is damaged Replace the ejector spacer The tip-ejector is loose assemble the tip-ejector properly The ejector lock is misaligned align the ejector lock Pipetting seize up Piston need lubricant Lubricate piston assembly However, if you can’t solve the problem, contact your Gilson distributor.
10 - Leak test
This test may be performed at any time to check that the pipette does not leak, especially after performing a main- tenance or decontamination procedure. If a pipette fails this test, replace the O-ring and seal. After making sure that the pipette is correctly reassembled, repeat this test.
For the P2g to P200g models:
Fit a PIPETMAN DIAMOND Tip.
Set the pipette to the maximum volume given in the specifications, and pre-rinse.
Aspirate the set volume from a beaker of distilled water.
Maintain the pipette in the vertical position and wait for 20 seconds.
If a water droplet appears at the end of the tip there is a leak.
If you see no droplet, re-immerse the tip below the surface of water.
The water level inside the tip should remain constant;
if the level goes down there is a leak.
For the P1000g, P5000g and P10mlg models:
Fit a PIPETMAN DIAMOND tip.
Set the pipette to the maximum volume given in the specifications.
Aspirate the set volume from a beaker of distilled water.
Maintain the pipette in the vertical position and wait for 20 seconds.
If a water droplet appears at the end of the tip, there is a leak.
11 - maIntenance
Routine maintenance will help keep your pipette in good condition, ensuring a continued high level of performance.
Maintenance is limited to:
Cleaning or decontamination (see Chapter 12 - Cleaning and Decontamination)
Replacing spare parts
Greasing the piston assembly.
PIPeTmaN P2G and P10G should not be disassembled, so you may only replace the push-button, tip-ejector, dual position tip-ejector and its adapter. With these pipettes if the tip-holder is damaged, the piston may also be damaged.
After replacing any parts you should verify the performance of your pipette following the verification procedure available on the Gilson website (www.gilson.com). If the pipette needs to be readjusted, please contact your local Gilson authorized Service Center.
changing the tip-ejector To remove
Push the ejection button.
Push laterally the tip-ejector.
Slide and remove the tip-ejector.
To refit
Push the ejection button.
Slide the tip-ejector along the tip-holder.
Clip the tip-ejector on the body of the pipette.
P10G, P20G
P100G, P200G P5000G
P10mlG P1000G
Tip-holder
Tip-ejector Connecting nut Color coded push-button
Piston assembly
Blue ring
changing the tip-holder – no tools required Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it counter- clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly and the seals.
Clean, autoclave, or replace the tip-holder.
If necessary, lubricate lightly the piston and its seals (see below).
Reassemble the pipette (refer to the figure, p 16).
Tighten the connecting nut (turn clockwise).
Refit the tip-ejector (see above).
servicing the Piston assembly
You may remove the piston assembly for cleaning pur- poses only. If the piston assembly is changed, the pipette must be adjusted and calibrated in a Gilson authorized Service Center. As the models P2G and P10G contain miniaturized parts, it is best not to disassemble these pipettes yourself.
The piston assembly must not be autoclaved.
Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it counter-clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly and the seals.
Leave exposed the piston, clean it with methyl alcohol and lubri- cate lightly. For P100G, P200G and P1000G models, lubricate only the useful part of the piston and the seals. For P5000G and P10mlG, lubricate the tip of the piston, disassemble the seals and
The use of other lubricant cancels the warranty of this pipette.
Reassemble the pipette (refer to the figure, page 13).
Tighten the connecting nut (turn clockwise).
Refit the tip-ejector (see above).
changing the seals
The O-ring and seal are on the piston; they must not be autoclaved, if worn or damaged in any way (chemical or mechanical), they must be replaced. As the models P2G and P10G contain miniaturized parts, it is best not to disassemble these pipettes yourself, please contact your local Gilson authorized Service Center.
The dimensions of the O-ring vary depending on the model of pipette.
Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it counter- clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly, O-ring and seal.
If necessary clean the piston and replace the seal;
lubricate them lightly. Please place them in the correct order.
Reassemble the pipette (refer to the figure, page 13).
Tighten the connecting nut (turn clockwise).
Refit the tip-ejector (see above).
