A/ Morphology of complementation strain Sp60
0.45 mM ZnCl
2
0 mM ZnCl
2
B/ Expression and phoshorylation of LocZ
D/ Complementation of growth time (min) 0 1 2 3 4 5 6 7 0 6 0 120 180 240 300 360 420 480 Sp1 Sp60 without ZnCl2 Sp60 0.25 mM ZnCl2 Sp60 0.45 mM ZnCl2 log 2 (OD 600 *100) kDa 80 58 - + Sp60 Sp57 Sp1 - + ZnCl2 StkP LocZ Sp60 Sp57 Sp1 a-LocZ a-pThr
C/ Cell size analysis
0 5 10 15 20 25 30 1 1,25 1,5 1,75 2 2,25 2,5 2,75 3 Sp60 WT Sp60 + ZnCl2 % of cells size classes (mm) PH DAPI Overlay