• Non ci sono risultati.

Chapter 1: Introduction ...7 


N/A
N/A
Info
Scarica
Protected

Academic year: 2021

Condividi "Chapter 1: Introduction ...7 
"

Copied!
3
0
0

Caricamento.... (Visualizza il testo completo ora)

Scarica adesso ( 3 pagine )

Testo completo

(1)

2

Index

Index ...2 


Abstract ...5 


Chapter 1: Introduction ...7 


1.1 Membrane remodelling and Microparticle formation ...7 


1.2 Microparticles in Intercellular Communication ...9 


1.2.1 Antigen transfer and intercellular cross-talk ...9 


1.2.2 Microparticles and vascular function ...9 


1.2.3 Microparticles in angiogenesis ...10 


1.3 Membrane Microparticles in Blood Coagulation...11 


1.3.1 Microparticles are essential for hemostasis ...11 


1.3.2 Microparticles and TF are closely associated in the vascular compartment 12 
 1.3.3 Microparticles, thrombosis, and inflammation...12 


1.4 The airway epithelium ...13 


1.4.1 Anatomy of the Respiratory Epithelium...14 


1.4.2 Epithelial Function...15 


1.4.2.1 Barrier function ...15 


1.4.2.2 Secretory function...15 


1.5 Inflammation...16 


1.5.1 Cytokines ...17 


1.5.2 Chemokines ...18 


(2)

3

1.5.3 Interleukine-8 (IL-8) ...19 


1.5.4 Monocyte chemotactic protein- 1 (MCP-1)...21 


1.6 Peroxisome Proliferatori Activated Receptors ...23 


1.6.1 Isoforms of PPARs ...24 


1.6.2 Structural features of PPARs ...24 


1.6.3 Gene transcription machinery ...25 


1.6.3.1 RXR and heterodimerisation...25 


1.6.3.2 Peroxisome proliferator response elements (PPREs) ...26 


1.6.3.3 Coactivators/corepressors (cofactors) ...26 


1.6.4 PPAR-α...26 


1.6.5 PPAR-δ/β ...27 


1.6.6 PPAR-γ...27 


1.6.6.1 15-Deoxy-Δ−12,14−prostaglandin J

2

...28 


1.6.6.2 Rosiglitazone ...28 


1.7 NF-κB ...28 


Chapter 2: Aim...30 


Chapter 3: Materials e Methods ...31 


3.1 Materials...31 


3.1.1 Reagents and Kits...31 


3.1.2 RT-PCR and Real Time Primers: ...31 


(3)

4

3.1.3 Cell culture...32 


3.2 Methods ...33 


3.2.1 Monocyte isolation and MP generation...33 


3.2.2 Viability Test ...34 


3.2.3 Epithelial cell activation by MP ...34 


3.2.4 Elisa for chemokine detection ...35 


3.2.5 Reverse transcriptase polymerase chain reaction ...35 


3.2.6 Real Time PCR...35 


3.2.7 Evaluation of NF-κB activation...36 


3.2.8 Preparation of nuclear and cytosolic cellular fractions...36 


3.2.9 Electrophoretic Mobility Shift Assay (EMSA)...37 


3.2.10 Data presentation and statistical analysis ...37 


Chapter 4: Results ...39 


4.1 MP-induced upregulation of chemokine synthesis by A549 cells is dependent on NF-κB activation. ...39 


4.2 PPAR-γ activation inhibits MP-induced synthesis of IL-8 and MCP-1 by airway epithelial cells. ...40 


Chapter 5: Discussion ...46 


Chapter 6: Conclusions...49 


Bibliography ...50 


Acknowledgments ...59 


Riferimenti

Scarica adesso ( PDF - 3 pagine - 64.86 KB )

Documenti correlati

Hedonic and utilitarian aspects of coffee consumption in China

After hedonic and utilitarian aspects of the F&B industry have been explored in the previous chapter, the second chapter focuses on the Chinese market and presents

Identifying the Nation

If we are to bring together normative theory with empirical work on the national question, the unit of analysis cannot, then, be the ‘national minority’ as an..

The International Monetary Policy of the German Central Bank 1958-1970

However, after a discussion in the Central Bank Council it accepted these requests almost unanimously.60 The German central bankers pointed to the fact that the Bundesbank had

International Schumpeterian competition and optimal R&D subsidies

the model is more suitable to study the effect of foreign competition coming from countries that are structurally not too different from the domestic one. As I will discuss later, if

Modeling of climate change effects on nutrients

The developed integrated modelling approach is made of 3 components: an ensemble of high- resolution climate projections used to describe the future climate conditions, the

Make me an offer I won't regret : offers to license works on acceptable terms cannot block libraries 'right' to digitize for access on dedicated terminals : Technische Universität Darmstadt

“Such acts of reproduction, unlike some operations involving the digitization of a work, also cannot be permitted under an ancillary right stemming from the combined provisions

"Il Rapporto annuale 2013 delle politiche e azioni contro il cambiamento climatico. Proposta di traduzione e commento"

Nello specifico a fronte della perdita dell’informazione fornita dal carattere bo 播 (seminare) è stato inserito “per l’irrigazione” per suggerire l’idea che l’acqua

On the 'entry options' for the 'right to love' : federalizing legal opportunities for LGBT movements in the EU

Moreover, the current multilevel system of fundamental rights protection in Europe (EU, Council of Europe, national states and a relatively vast number of actors such as