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Are pre-miR-146a and PTTG1 associated with papillary thyroid cancer?

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Supplementary table 1. Primers used to perform PCR, Sanger sequencing and High Resolution Melting.

 

SNP PRIMER SEQUENCES TECHNIQUE

 

rs2910164 (pre-miR-146a)  

    Forward: 5’-ATAACTCATGAGTGCCAGGAC-3’ PCR and sequencing     Reverse: 5’-TCACAGGAACTCACACTCCTT-3’ PCR and sequencing

 

rs1862391 (PTTG1)

    Forward: 5'-TAGAAACAGTGTCTTAGCCTGA-3' PCR and sequencing     Reverse: 5’-AAGCTGCATGGACTGAGGT-3’ PCR and sequencing

    Forward: 5'-ACAACCAATGTTGGGTCTAAA-3' HRM     Reverse: 5'-AAACGCTGCTGTTTGATCTA-3' HRM   rs2910202 (PTTG1)    

    Forward: 5'-CCCAGATGAGAGGACTTTGCTGT-3' PCR and sequencing     Reverse: 5'-GGGCATGAATCACCCAGGA-3' PCR and sequencing

    Forward: 5'-GGTGAGATAAAGCAGGAA-3' HRM

    Reverse: 5'-AGGTCTACTACAGTCTACTAT-3' HRM

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