• Non ci sono risultati.

Interaction between conservation tillage and nitrogen fertilization strongly shapes microbial diversity at different soil depths: a long-term study in the Mediterranean


Academic year: 2021

Condividi "Interaction between conservation tillage and nitrogen fertilization strongly shapes microbial diversity at different soil depths: a long-term study in the Mediterranean"


Testo completo






Elisa Pellegrinoa, Gaia Piazza, Marco Nuti, Laura Ercoli

Institute of Life Sciences, Scuola Superiore Sant’Anna, Piazza Martiri della Libertà 33, 56127 Pisa, Italy.

a Correspondence:Elisa Pellegrino: elisa.pellegrino@santannapisa.it

Soil biodiversity accomplishes key roles in agro-ecosystem services consisting in preserving and enhancing soil fertility and nutrient cycling, crop productivity and environmental protection. Thus, the improvement of knowledge on the effect of conservation practices, related to tillage and nitrogen (N) fertilization, on soil microbial communities is critical to a better understanding of the the role and function of microorganisms in regulating agro-ecosystems. In the Mediterranean basin, that is an area vulnerable to climate change and suffering for management-induced losses of soil fertility, the impact of conservation practices on soil microbial communities is paramount for building mitigation and adaptation strategies to climate change. A long-term experiment, originally designed to investigate the effect of tillage and N fertilization on crop yield and soil organic carbon, was utilized to understand the effect of these management practices on soil prokaryotic and fungal community diversity. The majority of prokaryotic and fungal taxa was common to all treatments at both soil depths, whereas few bacterial taxa (Cloacimonates, Spirochaetia and Berkelbacteria) and a larger number of fungal taxa (i.e., Coniphoraceae, Debaryomycetaceae, Geastraceae,

Cordicypitaceae and Steccherinaceae) were unique to specific management practices. Soil

prokaryotic and fungal structure was heavily influenced by the interaction of tillage and N fertilization: the prokaryotic community structure of the fertilized conventional tillage system was remarkably different respect to the unfertilized conservation and conventional systems in the surface layer. In addition, the effect of N fertilization in shaping the fungal community structure of the surface layer was higher under conservation tillage systems than under conventional tillage systems. Soil microbial community was shaped by soil depth irrespective of ploughing and N addition effect. The findings of this study gave new insights on identification of management practices supporting and suppressing beneficial and detrimental taxa, respectively, highlighting the importance of managing soil microbial diversity through agro-ecological intensified systems in the Mediterranean area.

Keywords: soil bacteria, soil fungi, soil archaea, arbuscular mycorrhizal fungi, Glomeromycota,

Illumina sequencing, 16S rRNA, ITS1 region


Documenti correlati

Food consumption tests inside the Ambisonics room with different soundscapes.. Effect of acoustical environment on food

Clinical scores collected at last follow-up will be compared with preoperative and the values of infraspinatus strength of the operated shoulder will be compared

The stalagmites, CC53, CC4 and CC27, were collected in the so-called "Galleria delle Stalattiti", a phreatic conduit in the Corchia Cave, placed ca.. The stable isotopes

Tilting propellers structure 21 The tilt angle doesn’t influence roll, pitch and yaw angles, which are still valid because they fully define the quad-rotor body orientation in

Fotogramma tratto dal video “A Medjugorje i momenti dell’Apparizione alla Veggente Mirjana - 2 marzo 2020”, caricato sul canale YouTube “Medjugorje tutti i giorni”,

La forza degli argomenti prodotti da Penia non sembra avere la funzione di persuadere il pubblico ma solo di aggravare il carattere di minaccia della maschera mostruosa verso la

Recently Regev, Silvermann & Shapiro [8] proposed the π-calculus [3] as a qualitative model of biochemical pathways, seen as network of proteins.. Then [6] use a stochastic

pro edure based on a geneti algorithm to dete t defe ts in a known host obje t..

• Il test di Mann-Whitney per dati non parametrici è stato utilizzato per valutare la differenza statistica la frequenza respiratoria dei soggetti prima di essere

The mass terms has two components, one universal !the strength of the background field" and one particle dependent, directly proportional to the coupling to the field itself..

I also administered free listing tasks to 27 individuals—representing a similarly composed sample of the local population—about the major components of Nature: plants,

In the late reaction, the denitrifying bacteria were gradually replaced by some anaerobes, and the removal rate of ammonia nitrogen, nitrite nitrogen and total

The overall objective of this thesis is (i) to understand the long-term potential of no-till (NT) for C sequestration in soils, exploring key environmental and

Early evaluation of cerebral metabolic rate of glucose (CMRglu) with 18F-FDG PET/CT and clinical assessment in idiopathic normal pressure hydrocephalus (INPH) patients before and

In this work, different techniques (intense and gentle flaming, mowing, tillage) were compared to establish weed control effectiveness in vineyards, focusing on the response of

Aim: To test the effect of linagliptin in non-obese diabetic (NOD) mice, a murine model of type 1 diabetes, to unveil a possible direct cardiovascular action of dipeptidyl peptidase

The difference was statistically significant for both PEDOT:PSS 1% EG and PEDOT:PSS 3% EG respect to the control (p<0.05,.. CHAPTER 4: Advanced Multifunctional Materials for

To this purpose, since 1990s, a considerable number of researchers built choice-sets based on optimal design theory (Kiefer and Wolfowitz, 1960; Fedorov, 1972). The construction

Per quanto concerne l’altezza (Graf.107) e il numero di foglie (Graf.108), non sono state evidenziate particolari differenze tra le coperture nell’arco di tutto

Table 3 - Values of anthocyanin contents, phenolic contents and total antioxidant capacity of coated cherries (cv Big Lory and Grace Star) at harvest and at the end after 21 days

However, the Government was deficient in finances and professional teachers, and this forced the state elementary schools to work analogously with the confessional schools for a

Tenendo come ipotesi-guida il senso attribuito dalla lettura freudiana al complesso della ‘melanconia’ e scendendo su un piano profondo di interpretazione ermeneutica, l’indagine