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Current status of Drosophila suzukii management in Trentino (Italy): research achievements and perspectives for sustainable control

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Scientific

Committee

Chad Finn,0regon State University, USA Massimo lorizzo, Fruitnet,com, USA Lara Giongo, Fondazione Edmund Mach, Italy Gianluca Savini, Sant'Orsola, Italy

Gianfranco Anfora, University of Trento, Italy Brian Farneti, Fondazione Edmund Mach,ltaly Francesco Emanuelli, Fondazione Edmund Mach, Italy

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5l Current status of llrosophila suzultii management in Trentino (Italyf, research achieuements and perspectiues for sustainable control

Gianfranco Anfora (L,2), Alberto Grassi (3), Marco Valerio Rossi Stacconi (3,4), Claudio loriatti (3) L Centre Agriculture Food Environment (C3A) Fondazione Edmund Mach / University of Trento

Z Research and Innovation Centre, Fondazione Edmund Mach, via E, Mach L, 38010 San Michele all'Adige, Trento, lta$ 3 Technology Transfer Centre, Fondazione Edmund Mach, via E, Mach 1",380L0 San Michele all'Adige, Trento,lta$ 4 Department ofHorticulture,Oregon State University, Corvallis,0regon, USA

Drosophila suzukii Matsumura (Diptera Drosophilidae) is an invasive alien insect in Europe that threats nany fruit crop production systems and has become nowadays the most impoltant pest 0n blueberry, To tackle this new issue, we started an aray of investigations aiming at unveiling the different aspects of the complex biology of the insect, assumed to be fundamental knowledge for developing alternative control methods, Particular attention was devoted to elucidate the issues regarding winter diapause in D, suzukii, The integration of evidences from behavioul morphology, and genetics increased our knowledge of D. suzukii overwinter diapause and highlighted the importance of early host plants and population bottleneck after diapause.

A survey to determine the presence, seasonal phenology and biological control status of indigenous parasitoid populations of D, suzukii was also carried out, Afterwards, the ability of the most effective pupal parasitoid, Trichopria drosophilae, to reduce early D, suzukii populations was assessed by means of augmentative releases with an area-wide approach. Results clearly showed a mitigation of the D. suzukiipopulation in the treated areas, associated with a higher T. drosophilae parasitism,

The active daily and seasonal dispersal capacity at either orchard scale or wider territorial range was also investigated, Results clearly indicate both dai$ movement from the crop to the surrounding woody vegetation and seasonal long migrations along altitudinal gradients, Our data suggest that these migrations have multiple functions for D, suzukii, including conferring the ability to exploit gradual changes of temperature, food, and ovipositional resources, as well as assist in the search for suitable overwintering sites in late autumn,

These observations willhelp direct more optimalpest management for this pest and could help bluebeny farmers and industries decide when, where and how to manage D, suzukii pest populations.

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îhe experience of Spotted hling llrosophila (Itrosophita suzukii| management in frentino on berry fruits Alberto Grassi

Technology Transfer Centre, Fondazione Edrnund Mach, via E, Mach L, 380L0 San Michele all'Adige,ltaly

In Trentino region (Northern ltaly), Drosophila suzukiiwas rletected for the first time in September 2009, As a first reaction, our local cherry and soft fruit growers increased the use of registered insecticides, but even if they used to spray many times during the rBening period, the efficacy in the control of the infestations showed to be unsatisfying. The development of alternative control measures appeared urgent t0 ensure an economic future for the concerned local fruit industry, The contribution of the Edmund Mach Foundation on this already started in 2010, with the organization and management of a territorialmonitoring, Data from the monitoring show an increasing demographic trend year after year, demonstrating that this smallregion of ltaly and Europe is likely one of the most infested areas in the world.

The research and experimental activity at FEM concentrated also on the development of control strategies, their contribution to the control of the infestation, particularly if they are applied on an area as wide as possible, must be considered essential,

Some agronómical praitices (e,g the use of a black mulching on the soil) tend to manipulate the plant growing habitat in order to create unsuitable conditiois for the pest rlevelopment. Sanitation and preventive measures (e,g short harvest intervals)may give a great contribution in the controlof the infestation on their own,

The mass trapping technique, through the containment of the immigrating adult population in a crop during the ripening period and particularly in the case of a low-medium pest pressure, showed to improve the controlwith insecticides.

Several experiences with insect-proof nets proved that this is current$ the most effective method to control the Spotted lrling Drosophila infestation on soft fruits, In Trentino, their use is particularly widespread in highbush bluebeny production, where the possibility to avoid the use of nylon rain protection coverings limits the dangerous over-heating of the microclimate into the orchard and its side-effects on the crop,

Recently, research and experimentation at the Edmund Mach Foundation are particularly focused on the development and evaluation of new approaches, like the biological control (by means of augmentative releases of indigenous pupal parasitoids), the use of Attract and Kill (ATK) devices and the Sterile Insect Technique (SIT),

Oueruiew of blueberry diseases in frentino Daniele Prodorutti

Technology Transfer Centre, Fondazione Edmund Mach, via E, Mach 1-,38010 San Michele all'Adige,ltaly

Compared to other fruit crops, blueberry is affected by few widespread diseases, In facts, most diseases of this crop are reported in specific growing areai or occur occasionally, Nevertheless, during the years, some pathogens causing damages on blueberry have been identified in Trentino region, Among them, plant showing poor growth and blight were found in highbush bluebeny (Vaccinium corymbosum) orchards, Symptomatic plants had rottedioots. Two Armillaria species (A. gallica and A, mellea)were identified as the causal agent of the disease, Armillaria spp, was identified on blueberry plants, on mulching barks spread on rows, on bark heaps and on fruit/forest trees at the field margins. Barks used as mulch and infected roots of óld trees could therefore act as inoculum source of the pathogen. In semi-field trials, Trichoderma strains resulted the most effective biocontrol agents against A, gallica and A, mellea. In the same area, highbush blueberry and rabbiteye blueberry (V. ashei) plants showed symptoms associated tó Bluebeny Scorch Virus (BlScV), Symptomatic plants resulted positive to BIScV in specific DAS-ELISA, Molecular analysis of the coat protein coding region demonstrated that the virus strain identified in Trentino was different from the strain previously identified in Piedmont (ruortn-west lialy)and similar to strains from Inlashington State (USA), Monitoring, controland prophylactic measures were carried out and to date the disease is under eradication, In the last years, wilting of highbush blueberry stems has been observed in some cultivation areas in Trentino, Brown discoloration of the wood and cankers were also observed, Fungibelonging to the family Botryosphaeriaceae were isolated from symptomatic stems, Further sturlies will be necessary to understand the biology and the epidemiology of these fungi and to find effective control methods,

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[7Pl Factors stimulating deuelopment of Ukrainian blueberries market in 2018 Levgenii Kharlan

PhD, CEO NikDaria LLC (TM iBeny),I2260Zhytomir, Ukraine

Ukrainian market of berries production is boosting with historic speed. Blueberries are considered as the main driver for berries market, As 0f 2018 there are up to L0 projects launched in Ukraine with more than 100 ha, while number of announced projects is by far larger.

Current high profitability of the blueberries production business (lRR>40%, ROl >50%), underdeveloped financial sectol as well as stagnatiqn of majority of economic sectors led to reallocation of funds available for investments towards agri sector, The berries market is not monopolized by large agriculture holdings, the entry barrier to the market is low Nowadays these theses are relevant even for farms less than i-0 ha.

Land plots to grow blueberries (PH 4-5) were not cultivated for many years, and now it allows to easi$ receive organic standard certificate for lands, and further to produced berries.

Finally, majority of larger market players are convinced that they willexport thefu volumes to internationalmarkets without tlifficulties, especially bearing in mind devaluation of localcurrency and low internalconsumer demand.

Consumer blueberries statistics both in Ukraine and in the world create optimism and illusion of infinite growth of the market, 0n top of that, mass media and produc-ers of young plants popularize active$ production of blueberries and organic products,

!ùorld grands like lrritec, Netafim, Talgil, FallCreak, Kusibab Wyka are operating in the market,

In 20L8 the government supported berries producers with B0% compensation of young plants costs, in addition EURZk per ha are considered for compensation of irrigation systems costs,

lnlhile there is strong tendency towards migration of labor resources outside the country, low labor costs are still considered as a strong competitive advantage. Irúorker's salary in field starts EUR I per business day: beny pickets are paid EUR 0,4 per L kg.

[8Pl Focus on the influence of substrate volume on deuelopment and the blueberry production in container Gianpiero Ganarin

Technology Transfer Centre, Fondazione Edmund Mach, via E, Mach L, 38010 San Michele all'Adige,ltaly

The cultivation of the Hightbush blueberry in Trentino normally takes place in the areas with favorable pedoclimatic characteristics.

In recent years there has been an interest in the production of blueberries also in areas that are unsuitable from the point of view of the lithological characteristics of the soils; in particular the pH that is too high for species with an optimal pH-range between 4,5-5,5,

The need therefore to carry out this experiment of soilless cultivation of Hightbush blueberry to investigate whether productivity is maintained as in plants on the ground and if the size of the containers can have an impact on the final product, The first results show a positive correlation between the containers size and the vegetative-productive data,

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