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FTIR-based multivariate models to forecast the tartaric stability

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(1)

fij ffi$

3w

" I ntegrando

Latinoamérica

a

través

de la vitivinicufù.raf'

(2)

COMITÉ ORGANIZADOR:

Jorge Baracatt Sabat - Fundación FAUTAPCI PRESIDENTE

Víctor Hugo Barrientos - Centro Nacional Vitivinícola (CENAVIT) VICEPRESIDENTE

WilmarVillena - Fundación FAUTAPO RESPONSABLE COM ISION CIENTíFICA

Mercedes Rojas - Asociación de Medianos Productores de Vinos y Singanis de Tarija (AMEVIT) RESPONSABLE COMISION DE LOGíSTICA

Karina Olarte Ouiroz - Fundacion FAUTAPO RESPONABLE COMISION DE COMUNICACION

GERENTE: María del Pilar Orozco

COMITE

CIENTIFICO:

/(" Wilmar Villena - Presidente Abel Furlén

Adolfo Aviles Adolfo Valentin Trigo Alvaro Pefra Beatriz Pugliese y' Eduardo Boido Eduardo Pattarino Estela de Frutos Franz Molina G i u l i a n o E l i a s P e r e i r a H e r n é n V i l a gr Hernín Ojeda lgor Arciénega llsen Patricia Castillo R. lvan Bluske

Joelsio José Lazzarotto Linder Espinoza Luigi Bavaresco Luis Antelo Bruno MaitiVan den Bosch # Nelson Sfarcich *. Philippo Pszczólkowski Raul Romero S a m a r V e l h o d a S i l v e i r a Victor Costa Viterman Velasquez Viviana Ugarte Yerko Sfarcich Bolivia Argentina Bolivia B o l i v i a C h i l e Argentina Uruguay U r u g u a y U r u g u a y B o l i v i a Brasil Argentina Francia Bolivia Bolivia B o l i v i a Brasil B o l i v i a I t a l i a Bolivia Argentina Bolivia C h i l e t . . ó o i l v r a Brasil C h i l e Bolivia B o l i v i a Bolivia Viticultura Enología Viticultura Alimentos Enología Viticultura Enología Economía Viticultura Enología Enología Viticultura Viticultura Viticultura Enología Enología Economía S u e l o s Viticultura Economía Economía Enología Viticultura Viticultura Viticultura Economía Enología Turismo Viticultura

(3)

X I V C O N G R E S O L A T I N O A M E R ] C A N O D E V I T I C U L T U R A Y E N O L O G í A

obtained from barrique staves, and with the effects of this treatment in terms of protection against the microbial contamination of wood and interterence with the phenol transfer to wine.

The membrane molecular structure was confirmed by solid state NMR. The fermentation kinetic of synthetic juices by Saccharomyces cerevisiae in presence of treaied or untreated wood, the ease of colonization of the tlocks by 15 species of wine spoilage yeasts and bacteria, and the transfer to wine of 21 simple phenols were studied.

Fermentation kinetic was unaffected. The membrane reduced the wood roughness, helped along washing and prevented wood colonization. Membrane hydrophobicity g1d the presence. of Sn could be involved in the process, the latter by the inhibition of the micro-bial growth inside the siliceous layer. No significant differences were observed for tÉe transfer of phenols, with the àxception of homovanillic acid. Now, these positive results obtained in laboratory scale suggest to examine in depth the membrane compatibility with alcohol before any winery application.

Keywords: oak wood, silica membrane, spoilage microorganisms, phenol extraction

ffirum*ffiw

O e n o l

ical tannins are ossible su

liers of varietal thiol

recu rsors

Roberto Larcherl, Loris Tonidandell, Bruno Fedrizzil, Giorgio Nicolini 1*

Centro Trasferimento Tecnologico, Fondazione E. Mach, v. E. Mach 1, 38010 San Michele all'Adige [N), ltalia' * g iorg io. n icol i n i@fmach. it; tel. +39-04 61 -61 51 1 1

Summary

Worldwide,

winemakers

use tannins

as clarifuing,

stabilising

and antioxidant

agents,

texturers

and aromatic

ad-juvants.

In this iast case,

the contribute

of tannins

is usually

traced back to their ability

of linking

sulphur

off-flavours

'and/orto

the supply

in vanilla-like,

spicy

and "oaky" aroma

compounds

mainly

related

to the Presence

of peculiar

simple

phenols

and lactones.

In this work we repoft that two thiol precursors

(3-S-cysteinyl

hexan-l-ol

and 3-S-glutathionyl

hexan-1-ol)

were

found for the first

time in grape commercial

oenological

iannin

samples,

in concentrations

as high as 200 and 138

'ng/kg, respectively.

Difterences

were found between

tannins

obtained

from grape skin and seeds,

in agreement

wiin tÉe distribution

of the precursors

in the grape berry reported

in the literature.

Technologically

speaking,

ad hoc additions

of selected

tannins

- afterfermentative

conversion

of the precursors

into the free thiol forms - .orld theoretically

rise

3-mercaptohexan-1-ol

over its sensory

threshold,

favour

the

forma-tion of the corresponding

acetate

during fLrmentation

and, finally,

improve

the exotic-fruit

flavour

profile

of wine.

Keywords:

wine, grape tannin,

varietal

thiols,

aroma precursors

ffirum*@ffi

FTIR-based

multivariate models to forecast the tartaric stabili

M a r i o M a l a c a r n e l , R o b e r t o L a r c h e r l , T o m é s R o m é n 1 , D a n i e l a B e r t o l d i r , G i o r g i o N i c o l i n i 1 *

1 Centro Trasferimento Tecnologico, Fondazione E. Mach, v. E. Mach 1, 38010 San Michele all'Adige fl-N), ltalia' * giorgio.nicolini@fmach.it; tel.+39-0461-615111

(4)

X I V c O N G R E S o L A T I N o A M E R I c A N O D E V I T I C U L Î U R A Y C T O I O C i A

5ummary

As the tartaric instability in the bottle still is a technical and commercial problem for winemakers, this paper investigated the possibility of forecasting the tartaric stability of wine using multivariate models created on the basis of infrared spectral information. More than 500 white, rosé and red wines - obtained without any addition of stabilizing agents - were analysed using Fourier Transform Infrared Spectroscopy, and their tartaric stability was assessed with the "mini-contact test" (10 min, 0'C, KHT) and the "cooling test" (5 days, -4"C), both used as well known and widespread reference methods.

Partial Least Squares-Regression and Artificial Neural Networks were applied on B0% of the samples to create predictive models which correctly classified (89 - 97%) the residual20"/" of wines used as external validation sub-set. ln the worst cases only 4-6% of unstable wines were erroneously òlassified as stable.

Keywords: wine, tartaric stability, FTIR, multivariate models, neural networks

ffifu#

ffi-ffiru

The young sparkling

wine aroma and its variability

due to the veast

strain and the amount of assimilable

nitrogen in the base-wine

Giorgio Nicolini 1*, Tomés Romén1, Sergio Moserl, Loris Tonidandell, Roberto Larcherl

Centro Trasferimento Tecnologico, Fondazione E. Mach, v. E. Mach 1, 38010 San Michele all'Adige |fN), ltalia. g iorg io. n icol in i@fmach. it; te l. +3 9 -0461 -61 51 1 1

Summary

Six base wines (3 Mueller-Thurgau,

2 Prosecco,

'l

Chardonnay),

each at 2 levels

of assimilable

nitrogen,

were

fermented

in the bottle with ó yeast

strains.

The yeasts

(SPóó5,

DV10, Rhone

2056, FR95,

BC, R2)

were prepared

as suggested

by the Comité Interprofessionnel

du Vin de Champagne.

The sparkling

wines

were kept on the lees

at 4-5"C.

About 40 aroma

compounds

in free form were analysed

by GC-FID

two months

after the end of second

fermentation.

Second

fermentation

did not increased

acetates

of higher alcohols

and ethyl esters

of fatty acids responsible

for fruitiness.

When pied de cuve is well prepared,

base

wine assimilable

nitrogen

is a marginal

factor

as regards

the fruity aroma compounds

produced

during second

fermentation.

The fruity aroma of sparkling

and fizzy

wines

to be drunk

young,

greatly

appreciated

by the consumers,

has

to be already

present

in the base

wine.

Keywords:

yeast

strain,

yeast

assimilable

nitrogen,

sparkling,

fermentative

aroma

compounds

ffiruffi-ffiffi

M o d e l a m i e n t o

y c o n t r o l d e u n a l a m b i q u e

charentais

de destilación

de vinos

Francisco Lopez-Bonillol, David Jopia-Castillo2, Ricardo Luna3, J. Ricardo Pérez-Coneaa, Francisco Cubillos2

'l

Depaftament d'Enginyeria Ouímica, Universitat Rovira iVirgili, Tarragona, Espafra. Tel: 34 977558503, e-ma i | : francisco. lopez@u rv.cat

2 Departamento de Ingeniería Ouímica, Universidad de Santiago de Chile, Santiago, Chile, david.jopia@usach.cl 3 Departamento de Ouímica, Universidad Tecnológica Metropolitana, Santiago, Chile, rich.aloonso@gmail.com 4 Departamento de Ingeniería Ouímica y Bioprocesos, Pontificia Universidad Católica de Chile, Santiago, Chile,

perez@ing.puc.cl.

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