Product name Anti-SHMT1 antibody Description Rabbit polyclonal to SHMT1
Host species Rabbit
Tested applications Suitable for: IP, WB
Species reactivity Reacts with: Mouse, Human
Predicted to work with: Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Immunogen Synthetic peptide within Human SHMT1 aa 453-483 (C terminal). The exact sequence is proprietary. (NP_004160.3).
Sequence:
AGDKYQAAVQALREEVESFASLFPLPGLPDF
Database link: P34896
Positive control Jurkat and NIH 3T3 whole cell lysates.
Form Liquid
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate pH 7 to 8
Purity Immunogen affinity purified
Purification notes ab186130 was affinity purified using an epitope specific to SHMT1 immobilized on solid support.
Clonality Polyclonal
Isotype IgG
Product datasheet Product datasheet
Anti-SHMT1 antibody ab186130 Anti-SHMT1 antibody ab186130
2 References 3 Images
Overview
Properties
Run BLAST with Run BLAST with
Function Interconversion of serine and glycine.
Pathway One-carbon metabolism; tetrahydrofolate interconversion.
Sequence similarities Belongs to the SHMT family.
Cellular localization Cytoplasm.
Western blot - Anti-SHMT1 antibody (ab186130)
Anti-SHMT1 antibody (ab186130) at 1 µg/ml + Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 3 minutes Applications
Our Abpromise guarantee covers the use of ab186130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/500 - 1/2500. Predicted molecular weight: 53 kDa.
Target
Images
Western blot - Anti-SHMT1 antibody (ab186130)
Anti-SHMT1 antibody (ab186130) at 1 µg/ml + NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 3 minutes
Immunoprecipitation - Anti-SHMT1 antibody (ab186130)
Detection of SHMT1 in immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186130 at 6 µg per reaction for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit https://www.abcam.com/abpromise or contact our technical team.
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Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
Product name Anti-PHGDH/Malate dehydrogenase antibody Description Mouse monoclonal to PHGDH/Malate dehydrogenase
Host species Mouse
Tested applications Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IP Species reactivity Reacts with: Human
Immunogen Recombinant full length protein corresponding to Human PHGDH/Malate dehydrogenase aa 1- 534.
General notes This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
This product was previously labelled as PHGDH
Form Liquid
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer pH: 7.20
Purity Tissue culture supernatant
Purification notes Purified from TCS.
Clonality Monoclonal
Isotype IgG1
Light chain type kappa
Product datasheet Product datasheet
Anti-PHGDH/Malate dehydrogenase antibody ab57030 Anti-PHGDH/Malate dehydrogenase antibody ab57030
6 References 5 Images
Overview
Properties
Applications
Our Abpromise guarantee covers the use of ab57030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Pathway Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
Involvement in disease Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.
Sequence similarities Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
Western blot - Anti-PHGDH/Malate dehydrogenase antibody (ab57030)
PHGDH/Malate dehydrogenase antibody (ab57030) at 1μg/ml + Jurkat cell lysate at 25μg/lane.
This image was generated using the ascites version of the product.
Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
Target
Images
Immunocytochemistry/ Immunofluorescence - Anti- PHGDH/Malate dehydrogenase antibody (ab57030)
PHGDH/Malate dehydrogenase antibody (ab57030) used in immunofluorescence at 1-5μg/ml on HeLa cells.
This image was generated using the ascites version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin- embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (ab57030)
PHGDH/Malate dehydrogenase antibody (ab57030) used in immunohistochemistry at 5μg/ml on formalin fixed and paraffin embedded human lymph node.
This image was generated using the ascites version of the product.
Immunoprecipitation - Anti-PHGDH/Malate dehydrogenase antibody (ab57030)
PHGDH/Malate dehydrogenase was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10µg of Mouse monoclonal to PHGDH/Malate dehydrogenase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70 C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab57030.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 57kDa; PHGDH/Malate dehydrogenase.
This image was generated using the ascites version of the product.
o
Flow Cytometry - Anti-PHGDH/Malate dehydrogenase antibody (ab57030)
Overlay histogram showing HeLa cells stained with ab57030 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57030, 1µg/1x10 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353,
2µg/1x10 cells) used under the same conditions. Acquisition of
>5,000 events was performed.
This image was generated using the ascites version of the product.
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit https://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
6
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Product name Anti-Decorin antibody [5E8E7]
Description Mouse monoclonal [5E8E7] to Decorin
Host species Mouse
Tested applications Suitable for: Flow Cyt, WB Species reactivity Reacts with: Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee
Immunogen Recombinant fragment corresponding to Human Decorin aa 263-324. (Expressed in E.coli).
Sequence:
GSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNN NISVVGSSDF CPPGHNTKKASY
Database link: P07585
Positive control Human Decorin recombinant protein; Decorin (aa: 263-324)-hIgGFc transfected HEK293 cell lysate; HEK293 cells.
General notes This product was changed from ascites to supernatant. Lot no’s high than GR150953-11 are from Tissue Culture Supernatant
Form Liquid
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.05% Sodium azide Constituent: 99% PBS
Contains 0.5% protein stabilizer (Amino acid =85%, pH(1% water solution) =7.0~7.5, Water =2%, As(mg/kg) =0.5, Pb(mg/kg) =0.1).
Purity Protein G purified
Purification notes Purified from tissue culture supernatant.
Product datasheet Product datasheet
Anti-Decorin antibody [5E8E7] ab181456 Anti-Decorin antibody [5E8E7] ab181456
1 References 3 Images
Overview
Properties
Run BLAST with Run BLAST with
Clonality Monoclonal
Clone number 5E8E7
Isotype IgG1
Function May affect the rate of fibrils formation.
Involvement in disease Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048].
Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
Sequence similarities Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats.
Post-translational modifications
The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
Cellular localization Secreted > extracellular space > extracellular matrix.
Applications
Our Abpromise guarantee covers the use of ab181456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
Flow Cyt 1/200 - 1/4000.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Predicted molecular weight: 40 kDa.
Target
Images
Western blot - Anti-Decorin antibody [5E8E7]
(ab181456)
Anti-Decorin antibody [5E8E7] (ab181456) at 1/500 dilution + Human Decorin recombinant protein
Predicted band size: 40 kDa
Expected MWt is 32.5 kDa.
Western blot - Anti-Decorin antibody [5E8E7]
(ab181456)
All lanes : Anti-Decorin antibody [5E8E7] (ab181456) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Decorin (aa: 263-324)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 40 kDa
Flow Cytometry - Anti-Decorin antibody [5E8E7]
(ab181456)
Flow cytometric analysis of HEK293 cells labeling Decorin with ab181456 at 1/200 dilution (green), compared to a negative control (red).
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit https://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
Product name Anti-Hsp75 antibody Description Rabbit Polyclonal to Hsp75
Host species Rabbit
Tested applications Suitable for: WB, IHC-P Species reactivity Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen Recombinant fragment corresponding to Human Hsp75 aa 405-704 (C terminal). (BC018950) Sequence:
LQESAL IRKLRDVLQQ RLIKFFIDQS KKDAEKYAKF FEDYGLFMRE GIVTATEQEV KEDIAKLLRY ESSALPSGQL TSLSEYASRM RAGTRNIYYL CAPNRHLAEH
SPYYEAMKKK DTEVLFCFEQ FDELTLLHLR EFDKKKLISV ETDIVVDHYK EEKFEDRSPA AECLSEKETE ELMAWMRNVL GSRVTNVKVT LRLDTHPAMV TVLEMGAARH FLRMQQLAKT
QEERAQLLQP TLEINPRHAL IKKLNQLRAS EPGLAQLLVD QIYENAMIAA GLVDDPRAMV GRLNELLVKA LERH
Database link: Q12931
Positive control K562, Jurkat and 293T cell lysate. Human colon cancer tissue.
Form Liquid
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer pH: 7.30
Preservative: 0.05% Sodium azide Constituents: 50% Glycerol, 49% PBS
Purity Immunogen affinity purified
Clonality Polyclonal
Product datasheet Product datasheet
Anti-Hsp75 antibody ab182775 Anti-Hsp75 antibody ab182775
2 Images
Overview
Properties
Run BLAST with Run BLAST with
Isotype IgG
Function Chaperone that expresses an ATPase activity.
Tissue specificity Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
Sequence similarities Belongs to the heat shock protein 90 family.
Cellular localization Mitochondrion.
Western blot - Anti-Hsp75 antibody (ab182775)
All lanes : Anti-Hsp75 antibody (ab182775) at 1/1050 dilution
Lane 1 : K562 cell lysate Lane 2 : Jurkat cell lysate Lane 3 : 293T cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Predicted band size: 80 kDa Applications
Our Abpromise guarantee covers the use of ab182775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 80 kDa.
IHC-P 1/100 - 1/200.
Target
Images
Immunohistochemistry (Formalin/PFA-fixed paraffin- embedded sections) - Anti-Hsp75 antibody (ab182775)
Immunohistochemistry of paraffin-embedded Human colon cancer tissue labeling Hsp75 using ab182775 at a 1/60 dilution.
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit https://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
