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Merck Life Science srl

Via Monte Rosa, 93 – 20149 Milano – Italy Tel 02 33417310

Fax 02 38010737 www.merckgroup.com

Società a socio unico soggetta a coordinamento e direzione di Merck Kgaa, Darmstadt, Germany

Codice Fiscale/Partita IVA IT 13209130155 Capitale Sociale i.v. Euro 600.000 – Socio Unico R.E.A. 1625731 – R.I. MI 218222/2000 Deutsche Bank

IBAN IT40 J 03104 01607 000000770016 SWIFT: DEUTITM1467

SAB4200548 Sigma-Aldrich

Anti-IDH1 (R132H) antibody, Mouse monoclonal

clone HMab-1, purified from hybridoma cell culture

Synonym: Anti-NADP(+)-specific ICDH, Anti-IDCD, Anti-IDH, Anti-IDP, Anti-IDPC, Anti- Isocitrate dehydrogenase 1 (NADP+), soluble, Anti-NADP-dependent isocitrate

dehydrogenase, cytosolic, Anti-NADP-dependent isocitrate dehydrogenase, peroxisomal, Anti-Oxalosuccinate decarboxylase, Anti-PICD, Idh1 R132H Antibody - Anti-IDH1 (R132H) antibody, Mouse monoclonal, Idh1 R132H Antibody

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Related Categories

Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nucleic Acids and Nucleic Acid Metabolism,

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conjugate unconjugated

clone HMab-1, monoclonal biological source mouse

application(s) western blot: 4.0-8.0 μg/mL using extract of HEK-293T cells overexpressing IDH1^R132H.

species reactivity human

mol wt antigen ~43 kDa

form buffered aqueous solution shipped in dry ice

storage temp. −20°C

antibody form purified immunoglobulin Quality Level 200

antibody product

type primary antibodies concentration ~1.0 mg/mL

Featured Industry Research Pathology UniProt accession

no. O75874

Gene

Information human ... IDH1(3417) Descrizione

General description

Anti-IDH1 (R132H) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma HMab1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to mutation R132H of human IDH1. A member of isocitrate dehydrogenase (IDH) family, IDH1, is the human cytoplasmic NADP-specific enzyme.

Its subcellular localization was shown to be in both peroxisomes and the cytoplasm.^[6]

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Immunogen

peptide corresponding to mutation R132H of human IDH1.

Application

Anti-IDH1 (R132H) antibody has been used:

• in immunoblotting

• in immunohistochemistry^[2]

• in western blotting^[1]

• in cell cycle analysis and apoptosis assays^[3]

• in chromatin immunoprecipitation (ChIP) assay^[3]

• in in vitro migration assay^[3]

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Isocitrate dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate into α α - KG) using NADP as co-substrate.^[4] Mutations in IDH1 are specific to Arg132 (R132) and endow them with the function of generating 2-hydroxyglutarate (2HG) instead of α-KG. This product alters gene transcription through effects on DNA and histone methylation. Several IDH1 mutations exist, including R132H, R132C, R132S, R132G and R132L.^[5] Each may result in different tumor type with varied malignant progression. The most frequent known mutation (>90%) is the alteration of arginine to histidine (R132H). Hence, antibodies that recognize the IDH1^R132H mutation can be useful for the diagnosis of mutation-bearing tumors like gliomas.^[3]

Sicurezza e Documentazione Informazioni sulla sicurezza RIDADR

NONH for all modes of transport

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MAK054 Sigma-Aldrich

α-Ketoglutarate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Proprieta' Related Categories

Assay Kits For Studying Glutamine Metabolism, Assay Kits by Specialty Area, Assay Kits for Studying Isocitrate Dehydrogenase (IDH) Activity, Cancer Metabolism, Cancer Research,

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usage sufficient for 100 colorimetric or fluorometric tests storage

temp. −20°C Descrizione

General description

α-Ketoglutarate (α-KG) is a key intermediate in the Tricarboxylic Acid (TCA) Cycle, formed by the oxidative decarboylation of isocitrate by isocitrate dehydrogenase. The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. α-KG is an important nitrogen transporter. This α-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying α-KG in a variety of samples.

Application

α-Ketoglutarate Assay Kit has been used to determine the activity of α-ketoglutarate.^[1][3][2]

Suitability

Suitable for the measurement of a-Ketoglutarate in a variety of biological samples including cell and tissue lysates

Principle

α-KG concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the α-KG present. Typical sensitivities of detection for this kit are 0.01 to 10 nmoles.

Sicurezza e Documentazione Informazioni sulla sicurezza RIDADR

NA 1993 / PGIII

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Anti-IDH1 (R132H) antibody, Mouse monoclonal

α-Ketoglutarate Assay Kit

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