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Cancer Vaccines Co-Targeting HER2/Neu and IGF1R

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Cancers 2019, 11, x S1 of S2

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Cancer Vaccines Co-Targeting HER2/Neu and IGF1R

Carla De Giovanni, Lorena Landuzzi, Arianna Palladini, Marianna Lucia Ianzano, Giordano Nicoletti, Francesca Ruzzi, Augusto Amici, Stefania Croci, Patrizia Nanni and Pier-Luigi Lollini Optimized sequence of the murine IGF1R gene

Optimized Sequence: Length: 4316, GC%: 50.65, Minimum Free Energy: 1519.80

AGTGAGGACTGAGTTGGAGACTTTTTTTTTCTTTTTTTCTTTTCTTTTTTTTTTTTTTTTCTAT TTTTGAGAAAAGGGAATTTCGTCCCAAATAAAAGGAGCCACCATGAAGAGTGGAAGTGGTG GAGGTAGCCCTACCAGTCTCTGGGGGCTGGTGTTTCTGTCAGCCGCTCTCAGCCTTTGGCCCA CCTCCGGGGAGATCTGTGGCCCCGGAATCGATATCAGAAACGATTACCAGCAGCTGAAAAG ACTGGAAAATTGCACCGTCATTGAGGGCTTCTTGCACATCCTGCTGATTTCCAAAGCTGAAG ACTATAGGTCATATAGGTTCCCTAAACTGACCGTGATAACAGAGTATCTGCTTCTGTTTCGCG TGGCCGGCCTGGAATCCCTGGGCGATCTGTTTCCTAATCTCACAGTGATCAGAGGCTGGAAG CTGTTCTACAACTACGCTCTGGTTATTTTCGAAATGACTAACCTGAAGGACATAGGGCTCTAC AACCTCAGGAACATCACCCGGGGCGCTATTCGGATCGAGAAAAATGCAGATCTGTGCTATCT GTCTACCATTGATTGGTCACTGATTCTGGATGCTGTCTCTAATAATTACATCGTGGGGAATAA GCCCCCTAAAGAATGCGGGGACCTTTGTCCTGGCACACTCGAGGAGAAACCAATGTGTGAA AAGACGACCATTAACAATGAGTATAACTATCGCTGTTGGACTACAAATAGATGCCAGAAGA TGTGCCCTTCTGTGTGTGGGAAGCGCGCATGTACCGAAAATAATGAGTGTTGTCACCCCGAA TGTCTTGGGTCCTGTCACACACCTGACGACAATACTACCTGTGTCGCCTGTAGGCATTATTAT TATAAAGGAGTTTGTGTTCCAGCCTGCCCCCCAGGGACTTATCGCTTTGAAGGCTGGCGGTGC GTGGACAGAGACTTTTGCGCTAACATTCCAAACGCCGAGTCCAGTGACTCCGATGGTTTCGT CATTCATGACGATGAATGTATGCAGGAATGCCCTAGCGGGTTTATACGGAACTCTACCCAGT CTATGTACTGTATTCCATGTGAAGGACCCTGTCCCAAAGTTTGTGGGGATGAAGAGAAGAAG ACAAAGACCATTGACAGCGTGACCTCAGCCCAGATGTTGCAGGGATGTACGATTCTGAAGG GAAATCTCCTCATCAACATTAGAAGGGGGAATAATATCGCAAGTGAACTGGAAAACTTTAT GGGACTGATAGAGGTCGTGACCGGGTATGTGAAGATTCGGCATAGCCACGCCCTTGTCTCTC TCAGCTTTCTCAAAAATCTGAGACTGATCCTGGGCGAGGAACAGCTTGAAGGTAACTACTCC TTTTACGTCCTCGACAACCAGAATCTGCAACAGCTGTGGGACTGGAACCACCGTAACCTCAC CGTGCGATCCGGAAAAATGTATTTTGCATTCAATCCAAAGCTGTGCGTTTCCGAGATCTACCG GATGGAAGAGGTCACGGGAACGAAGGGAAGACAGTCAAAAGGAGATATCAACACTAGGA ATAATGGAGAGCGGGCTTCCTGCGAAAGTGACGTGCTCAGATTCACCTCCACCACCACTTGG AAAAATCGAATTATCATTACGTGGCACCGTTATCGACCCCCTGATTATAGAGACCTGATCAG TTTCACCGTGTACTACAAGGAGGCTCCCTTTAAAAACGTTACAGAATACGACGGTCAGGATG CCTGCGGATCCAATTCATGGAATATGGTGGACGTGGATCTGCCTCCAAACAAAGAAGGGGA GCCAGGCATTCTTCTTCACGGCCTCAAACCGTGGACCCAGTATGCCGTCTATGTGAAGGCTGT GACCCTGACAATGGTCGAGAATGATCACATCCGGGGAGCCAAGTCTGAAATTCTGTACATTA GAACTAACGCTAGTGTCCCTAGTATCCCTCTCGACGTGCTGTCAGCCTCCAACTCTTCCAGCC AGCTGATCGTCAAATGGAACCCCCCGACTCTTCCGAACGGGAATCTGTCCTATTACATCGTG CGCTGGCAGAGGCAACCACAGGATGGGTATCTGTACCGGCACAACTATTGTAGTAAGGATA AAATTCCAATTAGGAAGTATGCAGATGGGACCATTGACGTGGAGGAGGTTACTGAGAACCC CAAGACAGAGGTTTGTGGAGGGGATAAGGGACCGTGTTGTGCCTGCCCCAAGACAGAGGCC GAAAAACAGGCCGAAAAGGAGGAAGCTGAGTATCGCAAGGTCTTCGAGAACTTCCTTCATA ACTCTATCTTTGTCCCCAGGCCTGAACGTAGACGGAGAGATGTGATGCAAGTGGCCAATACG ACCATGTCTAGTAGGAGTCGCAATACTACCGTCGCCGACACATATAACATCACAGACCCGG AGGAGTTCGAGACTGAATATCCCTTCTTTGAGAGCAGGGTGGACAATAAGGAAAGAACAGT GATCAGCAACCTGCGACCGTTCACACTGTACAGGATCGATATTCACTCCTGTAACCACGAAG CCGAGAAGCTGGGCTGCAGTGCAAGTAACTTTGTCTTCGCCCGAACTATGCCCGCAGAGGGT

(2)

Cancers 2019, 11, x S2 of S2

GCCGACGATATTCCCGGCCCCGTTACCTGGGAACCGAGGCCCGAGAACTCTATCTTCCTCAA ATGGCCAGAGCCCGAGAACCCTAATGGTCTTATCCTGATGTATGAGATCAAGTACGGCAGCC AGGTGGAAGACCAGAGAGAATGTGTCTCCAGGCAAGAATACAGGAAGTATGGTGGGGCAA AACTGAACCGGTTGAATCCGGGAAATTATACTGCCAGGATCCAAGCCACTTCTCTCAGTGGC AATGGGTCCTGGACAGATCCTGTGTTCTTCTACGTGCCAGCCAAGACAACCTATGAAAACTT TATGCACCTCATTATTGCCCTCCCAGTTGCTATCCTCCTTATCGTGGGTGGACTGGTCATCATG CTTTATGTCTTTCACCGGAAGCGCAACAATAGTAGACTGGGTAACGGCGTCCTGTATGCTAGT GTCAATCCGGAGTACTTTTCAGCAGCTGACGTCTACGTGCCTGATGAGTGGGAAGTGGCCCG CGAAAAAATCACCATGAACAGAGAACTGGGCCAGGGCAGCTTTGGAATGGTCTATGAGGGT GTCGCTAAAGGAGTCGTGAAGGACGAGCCAGAAACTCGGGTGGCCATTAAGACTGTGAATG AAGCCGCTTCTATGAGGGAGAGAATCGAATTTCTCAACGAGGCCAGCGTCATGAAGGAGTT CAATTGTCACCATGTTGTGCGGCTCCTTGGCGTGGTCAGTCAGGGCCAGCCGACACTTGTTAT CATGGAGCTGATGACCCGTGGGGACCTCAAGTCCTACCTGCGATCTCTCCGACCGGAAGTTG AGCAGAACAATCTGGTCCTGATACCCCCTAGTCTGAGCAAGATGATTCAGATGGCCGGAGA AATTGCCGATGGAATGGCCTATCTGAACGCCAACAAATTCGTGCATAGGGATCTTGCAGCTC GAAATTGCATGGTGGCTGAGGATTTCACTGTGAAGATCGGAGACTTTGGCATGACAAGGGAC ATCTACGAAACAGACTACTATCGCAAGGGAGGCAAAGGCCTGCTTCCAGTTCGGTGGATGA GCCCCGAGTCACTGAAAGATGGTGTCTTCACCACCCACTCAGATGTCTGGAGCTTTGGAGTG GTCCTGTGGGAAATCGCCACCCTGGCTGAACAGCCATACCAGGGCTTGTCCAACGAGCAGGT TCTGCGATTCGTCATGGAGGGGGGCCTCTTGGACAAGCCTGACAACTGCCCCGATATGCTGT TCGAACTGATGAGAATGTGCTGGCAATACAACCCCAAGATGCGACCGTCTTTCCTGGAGATA ATTGGGTCTATCAAGGATGAGATGGAACCCAGCTTCCAGGAGGTGAGCTTCTATTATAGCGA GGAAAACAAACCTCCCGAGCCAGAGGAACTGGAGATGGAGCCAGAGAATATGGAGTCAGT TCCACTCGACCCTAGCGCCAGCAGTGCCTCACTCCCTCTCCCTGAACGACACAGTGGGCACA AGGCAGAGAACGGCCCAGGTCCGGGGGTTCTTGTGCTTAGAGCCTCTTTTGATGAGCGGCAG CCATATGCACACATGAATGGGGGGAGAGCCAACGAGCGCGCTCTGCCTCTGCCACAGAGCT CTACCTGCTGATCCTCGGACACACCGAAGCACGCGCCAACAGTAACGTGTGTGCGCCCACTC G G T G G G C G G G G G G G C G G G G A G G G G A G A G C A G G T T G T A A C A A T C T A T T C A

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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