• Non ci sono risultati.

A novel bacterial flavoenzyme (BVMO) for biotechnological applications

N/A
N/A
Protected

Academic year: 2021

Condividi "A novel bacterial flavoenzyme (BVMO) for biotechnological applications"

Copied!
1
0
0

Testo completo

(1)

A novel bacterial flavoenzyme (BVMO) for biotechnological applications

Ivan Zgrablic, Sheila J. Sadeghi, Daniela Minerdi and Gianfranco Gilardi

Department of Human and Animal Biology, University of Torino, Italy

Baeyer-Villiger monooxygenases (BVMOs) are flavin-containing enzymes that catalyse a wide variety of oxidative reactions specifically on the carbonyl moiety of substrates, many of which are difficult to achieve using chemical approaches. Moreover, these proteins have great value in the areas of bioremediation and green chemistry.

A novel BVMO gene in Acinetobacter radioresistens S13 was identified and using Clustal V its amino acid sequence was aligned with known BVMOs. Phylogenetic analysis demonstrated that it clustered together with BVMO from Mycobacterium, distant from the classical cycloalkanone MOs.

After cloning and successful expression in E.coli, the BVMO protein was purified in high yields (20mg/L culture) using Nickel affinity chromatography. Preliminary characterisation showed a 57 kDa soluble protein with a tightly but non-covalently bound FAD. Due to the large substrate specificity of BVMOs, a 3D homology model of this enzyme was generated in order to select its putative substrates using in silico docking. Well-defined substrate profiles are available for several BVMOs and together with our model this may allow careful predictions for substrates of this novel enzyme.

Riferimenti

Documenti correlati

S1 (B) display a high magnification SE-SEM cross sectional image of Er-doped PSi layer: the measured thickness of the porous layer is about 1.3 µm, and in order to understand

The cluster representatives (see respectively Supplementary Figures  S5 and S6 for the sampling of AP and DP clusters in AcrB and AcrD along the MD simulations) extracted from

Let G (n) be any gate defined with respect to the canonical truth-perspective.. 3 Roughly speaking, we might say that the holistic conjunction defined on a global in- formation

CT: Computed tomography; EUS: Endoscopic ultrasound; FNA: Fine-needle aspiration; HIFU: High-intensity focused ultrasound; IRE: Irreversible electroporation; MMFI: Multimodal

When µ assumes high values, negative effect on transportation is not counterbalanced enough by the inventory carrying cost savings so excessive batch size reduction leads to

GC×GC- TANDEM IONIZATION-TOFMS WITH COMBINED UNTARGETED AND TARGETED (UT) FINGERPRINTING OF VOLATILES FROM EXTRA VIRGIN OLIVE OIL: CHALLENGES FOR DATA ALIGMENT AND

In this work, ligand binding of structurally different ligands to the mouse Aryl hydrocarbon Receptor (mAhR) homology model is analyzed.. 3f1n 3f1p 3f1o 4zp4 4zqd 4gs9 4xt2 3h82

We focused our attention on the applications of Thermal Desorption Spectroscopy (TDS) to High-Energies Physics, Astrophysics and Geophysics; in fact this technique was