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Towards high throughput phenotyping of the multisensory space of apple quality

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(1)6thRosaceousGenomicsConference Mezzocorona,Italy–30thSeptemberͲ04thOctober2012  . .  MarkervalidationforRpf1redsteleresistanceinstrawberry  ǤǤƒ–Š‡›(1)ǡ ƒ‹‡•‘ǤǤ(2)ǡ˜ƒ†‡‡‰ǤǤ(3)ǡN.V.Bassil(4)ǡ ƒ ‘  Ǥ Ǥ(5)ǡ ‹ ǤǤ(4)  ȋͳȌ”‡‰‘–ƒ–‡‹˜‡”•‹–›ǡ‘”˜ƒŽŽ‹•ǡǡ ȋʹȌ‰”‹ ǤƬ‰”‹Ǧ ‘‘†ƒƒ†ƒǡ‡–˜‹ŽŽ‡ǡǡƒƒ†ƒ ȋ͵Ȍƒ‰‡‹‰‡ǦŽƒ–”‡‡†‹‰ǡŠ‡‡–Š‡”Žƒ†• ȋͶȌǦǡ ǡ‘”˜ƒŽŽ‹•ǡǡ ȋͷȌ‹ Š‹‰ƒ–ƒ–‡‹˜‡”•‹–›ǡƒ•–ƒ•‹‰ǡ

(2) ǡ  ‡† •–‡Ž‡ ‹• ƒ †‡˜ƒ•–ƒ–‹‰ ”‘‘– ”‘– †‹•‡ƒ•‡ ‹ •–”ƒ™„‡””‹‡•Ǥ ‡˜‡”ƒŽ •‘—” ‡• ˆ‘” ‰‡‡–‹  ”‡•‹•–ƒ ‡ ƒ”‡ ‡š’Ž‘‹–‡† ‹ „”‡‡†‹‰ǡ ƒ† •‡˜‡”ƒŽ ”ƒ ‡Ǧ•’‡ ‹ˆ‹  Ǧ‰‡‡• ™‡”‡ †‹•–‹‰—‹•Š‡†Ǥ ‡ ‡–Ž›ǡ ƒ –‹‰Š–Ž› Ž‹‡†  ƒ”‡” ™ƒ• ˆ‘—† ˆ‘” –Š‡ ’ˆͳ ‰‡‡ ƒ– ƒ‰‡‹‰‡ǦǡŠ‡‡–Š‡”Žƒ†•Ǥ‡Š—†”‡†ƒ†ˆ‘”–›‹‡‹†‹˜‹†—ƒŽ•™‹–Š‘™ ƒ†—‘™”‡•’‘•‡–‘–Š‹•’ƒ–Š‘‰‡ǡŠ›–‘’Š–Š‘”ƒˆ”ƒ‰ƒ”‹ƒ‡ǡ ™‡”‡–‡•–‡†‹„‡ Š –‡•–•ˆ‘””‡•’‘•‡–‘–™‘”ƒ ‡•‘ˆ–Š‹•†‹•‡ƒ•‡ǣƒƒ†‹ƒ”ƒ ‡ͶȋǦ͵Ȍ‹•‘Žƒ–‡Ǧ͵ǡƒ† †ǦͷȋǦͷȌ‹•‘Žƒ–‡•Ǧʹ͵ƒ†Ǧ͹͹™Š‡”‡’ˆͳ ‘ˆ‡”•”‡•‹•–ƒ ‡–‘”ƒ ‡Ͷƒ†‹• ‹‡ˆˆ‡ –‹˜‡ ƒ‰ƒ‹•– ”ƒ ‡ †ǦͷǤ ™‡–›Ǧ‹‡ ‹†‹˜‹†—ƒŽ• ‘•‹•–‹‰ ‘•–Ž› ‘ˆ ™‹Ž† ƒ ‡••‹‘•‘””‡ ‡–†‡”‹˜ƒ–‹˜‡•‘ˆ ”‘••‡•™‹–Š™‹Ž†”‡Žƒ–‹˜‡•™‡”‡‹†‡–‹ˆ‹‡†ƒ•Šƒ˜‹‰ ‘–Š‡”ǡ’‘–‡–‹ƒŽŽ›‡™ˆƒ –‘”•‘ˆ”‡•‹•–ƒ ‡„›‡šŠ‹„‹–‹‰”‡•‹•–ƒ ‡–‘”ƒ ‡Ǧͷƒ†ƒ› „‡˜ƒŽ—ƒ„Ž‡ˆ‘”™‹†‡‹‰–Š‡‰‡‡–‹ „ƒ•‡‘ˆ”‡•‹•–ƒ ‡‹ ‘‡” ‹ƒŽ —Ž–‹˜ƒ”•Ǥ‘ƒ˜‘‹† ‡’‹•–ƒ–‹  ‡ˆˆ‡ –•ǡ –Š‡•‡ ‹†‹˜‹†—ƒŽ• ™‡”‡ ‡ š Ž—†‡† ˆ”‘ ˜ƒŽ‹†ƒ–‹‘ ‘ˆ –Š‡ ’ˆͳ ƒ”‡”Ǥ ‘” –Š‡ͳʹͲ ‹†‹˜‹†—ƒŽ• –Šƒ– •Š‘™‡† Š‹‰Š †‹•‡ƒ•‡ • ‘”‡• ˆ‘” †Ǧͷǡ Ͷͳ •Š‘™‡† ƒ† ͹ͻ Žƒ ‡† –Š‡ ƒ”‡” ƒŽŽ‡Ž‡Ǥ ”‡Ž‹‹ƒ”› ƒƒŽ›•‹• ˆ‘” ‘””‡Žƒ–‹‘• „‡–™‡‡ ƒ”‡” ƒ† †‹•‡ƒ•‡ • ‘”‡• ”‡˜‡ƒŽ‡† ͵Ͳ †‡˜‹ƒ–‹‘•ǡ ͳ͹ ‹†‹˜‹†—ƒŽ• „‡‹‰ •—• ‡’–‹„Ž‡ †‡•’‹–‡ Šƒ˜‹‰ –Š‡ ƒ”‡” ƒ† ͳ͵ ‹†‹˜‹†—ƒŽ• Šƒ˜‹‰ Ž‘™ †‹•‡ƒ•‡ • ‘”‡• †‡•’‹–‡ Žƒ ‹‰ –Š‡ ƒ”‡”Ǥ ƒ—•‡• ˆ‘” –Š‡ Ž‘™‡” ȋ͹ͷΨȌ –Šƒ ’”‡˜‹‘—•Ž› ‘„•‡”˜‡† ȋͻͻΨȌ ƒ”‡” –‘ †‹•‡ƒ•‡ ‘””‡Žƒ–‹‘•ƒ”‡„‡‹‰‹˜‡•–‹‰ƒ–‡†Ǥ  Towardshighthroughputphenotypingofthemultisensoryspaceofapplequality ‹ƒ•‹‘Ž‹ Ǥ(1)ǡ’”‡ƒǤ(1)ǡ‡––ƒǤ(1)ǡƒ’’‡ŽŽ‹Ǥ(1)ǡ‘”‘ŽŽƒ”‘ǤǤ(1)ǡ‘•–ƒ Ǥ(1)ǡ‡ƒ––° Ǥ(1)ǡ†”‹œœ‹

(3) Ǥ(1)ǡ‘ƒ‘Ǥ(1)ǡ‹‰Ǥ(1)ǡ ǤGasperi(1) (1)‡•‡ƒ” Šƒ†

(4) ‘˜ƒ–‹‘‡–”‡ǡ ‘†ƒœ‹‘‡†—†ƒ Šǡ͵ͺͲͳͲƒ‹ Š‡Ž‡. ƒŽŽ̵†‹‰‡ȋȌǡ

(5) –ƒŽ›. Š‡’‡” ‡‹˜ƒ„Ž‡“—ƒŽ‹–›‘ˆƒ’’Ž‡‹•‘‡‘ˆ–Š‡‘•–‹’‘”–ƒ–ƒ•’‡ –•–‘„‡ ‘•‹†‡”‡†‹ „”‡‡†‹‰ǡˆ‘”–Š‡•‡Ž‡ –‹‘ƒ•™‡ŽŽƒ•–Š‡ ‘‡” ‹ƒŽ‹œƒ–‹‘‘ˆ‡™ƒ ‡••‹‘•Ǥ‡•‘”› • ‹‡ ‡ ƒŽŽ‘™• –Š‡ †‡ˆ‹‹–‹‘ ƒ† ‘–”‘Ž ‘ˆ –Š‡ —Ž–‹†‹‡•‹‘ƒŽ •’ƒ ‡ „‡Š‹† –Š‡ ’‡” ‡‹˜‡† “—ƒŽ‹–› ‘ˆ ƒ’’Ž‡ǡ „—– ‹– ‹• —•—ƒŽŽ› ‡š’‡•‹˜‡ ƒ† –‹‡ ‘•—‹‰Ǥ ‘” –Š‹• ”‡ƒ•‘–Š‡‹’Ž‡‡–ƒ–‹‘‘ˆ•‡•‘”›ƒ––”‹„—–‡•ƒ•’Š‡‘–›’‹ †‡• ”‹’–‘”•ˆ‘”‰‡‘‹  ‹˜‡•–‹‰ƒ–‹‘‹•ǡ‹‰‡‡”ƒŽǡ†‹ˆˆ‹ —Ž–Ǥ

(6) ʹͲͳͲ™‡•–ƒ”–‡†ƒŽƒ”‰‡•‡•‘”›Ȁ‹•–”—‡–ƒŽ’Š‡‘–›’‹‰ƒ –‹˜‹–›‘•‡˜‡”ƒŽƒ’’Ž‡ „ƒ•‡†‘‹‘˜ƒ–‹˜‡–‡ Š‘Ž‘‰›ƒ†—Ž–‹†‹• ‹’Ž‹ƒ”›‘™Š‘™–‘†‡ˆ‹‡‘†‡Ž•–Šƒ–ǡ . ͹ͺ.

(7) 6thRosaceousGenomicsConference Mezzocorona,Italy–30thSeptemberͲ04thOctober2012  . . ‘–Š‡„ƒ•‹•‘ˆ”ƒ’‹†‹•–”—‡–ƒŽ Šƒ”ƒ –‡”‹œƒ–‹‘ǡ ƒ’”‘˜‹†‡”‡Ž‹ƒ„Ž‡“—ƒ–‹ˆ‹ ƒ–‹‘‘ˆ ‡› •‡•‘”› –”ƒ‹–• ‘ Žƒ”‰‡ †ƒ–ƒ •‡–• –‘ „‡ —•‡† ‹ ‰‡‘‡ ™‹†‡ ƒ••‘ ‹ƒ–‹‘ ƒƒŽ›•‹•ǡ ’ƒ”–‹ —Žƒ”Ž›‡Ž— ‹†ƒ–‹‰–Š‡‰‡‡–‹ „ƒ•‹•‘ˆˆ”—‹––‡š–—”‡ƒ†ˆŽƒ˜‘””‡Žƒ–‡†–”ƒ‹–•Ǥ ‡”‡ ™‡†‡• ”‹„‡–Š‡‰‡‡”ƒŽˆ”ƒ‡‘ˆ‘—”’”‘Œ‡ –ƒ†–Š‡”‡•—Ž–•‘„–ƒ‹‡†ƒ•ˆ‘ŽŽ‘™•ǣ‹Ȍ–Š‡ •‡– —’ ‘ˆ ƒ ‘’”‡Š‡•‹˜‡ •‡•‘”› ‡–Š‘†‘Ž‘‰› –‘ ’”‘˜‹†‡ ”‡Ž‹ƒ„Ž‡ǡ —‹ˆ‹‡† ƒ† ‘’‡–‹–‹˜‡•‡•‘”› Šƒ”ƒ –‡”‹œƒ–‹‘ǡ‹‹Ȍ–Š‡•‡––‹‰ƒ†˜ƒŽ‹†ƒ–‹‘‘ˆ‘†‡Ž•–‘‡•–‹ƒ–‡ •‡•‘”› ƒ––”‹„—–‡• „ƒ•‡† ‘ ‹•–”—‡–ƒŽ †ƒ–ƒǡ ‹‹‹Ȍ –Š‡ •–—†› ‘ˆ –Š‡ ‘’Ž‡š‹–› ‘ˆ –Š‡ ‹–‡”ƒ –‹‘ „‡–™‡‡ ’”‘†— – ƒ† ‘•—‡” †—”‹‰ ƒ’’Ž‡ ˆ”—‹–• ‘•—’–‹‘ „› ‘•‡ •’ƒ ‡ ƒƒŽ›•‹• ƒ† ’•› Š‘Ǧ’Š›•‹ ƒŽ ‡š’‡”‹‡–• ƒ† ‹˜Ȍ –Š‡ ‹˜‡•–‹‰ƒ–‹‘ ‘ˆ –Š‡ Ž‹ ™‹–Š‰‡‘‹ ‹ˆ‘”ƒ–‹‘Ǥ  Use of genotypingǦbyǦsequencing for quantitative trait loci mapping of chilling requirementandbloomdateinpeach  ƒ—ŠǤ(1)ǡ ƒǤ(2)ǡ„„‘––Ǥ Ǥ(3)ǡ ƒ•‹ Ǥ(1)ǡ‹‡ǤǤ(4)ǡD.G.Bielenberg(1)  ȋͳȌ Š‘‘Ž‘ˆ‰”‹ —Ž–—”ƒŽǡ ‘”‡•–ǡƒ†˜‹”‘‡–ƒŽ ‹‡ ‡•ǡŽ‡•‘‹˜‡”•‹–›ǡ Ž‡•‘ǡ‘—–Šƒ”‘Ž‹ƒʹͻ͸͵Ͷ ȋʹȌ ‡Žˆ‡•‡ƒ” Š

(8) •–‹–—–‡‘ˆ —ƒ ‡‡–‹ •ǡ ”‡‡™‘‘† ‡‡–‹ ‡–‡”ǡ ”‡‡™‘‘†ǡ ‘—–Šƒ”‘Ž‹ƒʹͻ͸Ͷ͸ ȋ͵Ȍ

(9) Ǧ‘”†‡ƒ—šǡ ”ƒ ‡ ȋͶȌǦǡ‘—–Š‡ƒ•–‡” ”—‹–ƒ†”‡‡—–‡•‡ƒ” Šƒ„‘”ƒ–‘”›ǡ›”‘ǡ ‡‘”‰‹ƒ ͵ͳͲͲͺ  Ž‘‘ †ƒ–‡ ȋȌ ƒ† Š‹ŽŽ‹‰ ”‡“—‹”‡‡– ȋȌ ƒ”‡ ‡ ‘‘‹ ƒŽŽ› ‹’‘”–ƒ– –”ƒ‹–• ˆ‘” „”‡‡†‹‰ ˆ”—‹– ƒ† —– –”‡‡ •’‡ ‹‡• ƒ†ƒ’–‡† –‘ Ž‘ ƒŽ Ž‹ƒ–‡ ‘†‹–‹‘•Ǥ ‡ Šƒ˜‡ †‡˜‡Ž‘’‡†ƒ’‡ƒ Š ʹƒ’’‹‰’‘’—Žƒ–‹‘™‹–Šͷ͹‰‡‘–›’‡•ˆ”‘ƒ•‡Žˆ‡† ͳ’”‘‰‡› ‘ˆ ƒ ”‘•• „‡–™‡‡ Ǯ ƒ—Š‘ǯ ȋŠ‹‰Š Ȍ ƒ† Ǯ ‘Ž†ǯ ȋŽ‘™ ȌǤ ‡ • ‘”‡†  ‹ •‡˜‡ ›‡ƒ”•Ǣ  ™ƒ• ‡˜ƒŽ—ƒ–‡† ‘˜‡” –™‘ ™‹–‡” •‡ƒ•‘• „› • ‘”‹‰ „—† „”‡ƒ ‘ˆ ˆ‘” ‡† —––‹‰•Ǥ

(10) ‹–‹ƒŽ ƒ”‡” • ”‡‡‹‰ ‹†‡–‹ˆ‹‡† ͵͹ ’‘Ž›‘”’Š‹  •‹’Ž‡ •‡“—‡ ‡ ”‡’‡ƒ– ȋȌƒ”‡”•†‹•–”‹„—–‡†ƒ ”‘••ƒŽŽŽ‹ƒ‰‡‰”‘—’•ȋ ȌǤ

(11) ‘”†‡”–‘•ƒ–—”ƒ–‡‘—”‰‡‡–‹  ƒ’ ™‹–Š ƒ”‡”•ǡ ™‡ –‡•–‡† ƒ ‰‡‘–›’‹‰Ǧ„›Ǧ•‡“—‡ ‹‰ ȋ Ȍ ƒ’’”‘ƒ Š –‘ †‹• ‘˜‡” •‹‰Ž‡ — Ž‡‘–‹†‡ ’‘Ž›‘”’Š‹•• ȋȌ ’”‡•‡– ‹ –Š‡ ’‘’—Žƒ–‹‘Ǥ ‡‘‹   ™ƒ• ’‡

(12) ”‡•–”‹ –‡†ǡŽ‹‰ƒ–‡†–‘„ƒ” ‘†‡†ƒ†ƒ’–‘”•ǡƒ†’‘‘Ž‡†ƒ† ƒ’Ž‹ˆ‹‡†ˆ‘”—Ž–‹’Ž‡š •‡“—‡ ‹‰ ‘ –Š‡

(13) ŽŽ—‹ƒ ‹‡“ ʹͲͲͲ ’Žƒ–ˆ‘”Ǥ ’’”‘š‹ƒ–‡Ž› ͳ͸Ͳ ‹ŽŽ‹‘ •‡“—‡ ‡ ”‡ƒ†• ™‡”‡ ’”‘ ‡••‡† —•‹‰  ͵ǤͲǤ • ™‡”‡ ˆ‹Ž–‡”‡† –‘ ”‡–ƒ‹ ‘Ž› Ž‘ ‹ Š‘‘œ›‰‘—• ™‹–Š‹ ‡ƒ Š ’ƒ”‡–ǡ „—– ’‘Ž›‘”’Š‹  „‡–™‡‡ ’ƒ”‡–•Ǥ Š‹• ‘•‡”˜ƒ–‹˜‡ ‰‡‡–‹  Ž‹ƒ‰‡ ƒ’ ‘•‹•–‹‰ ‘ˆ ͵͹ • ƒ† ε͵ͺͲ • ‹ ‡‹‰Š– Ž‹ƒ‰‡ ‰”‘—’• ™ƒ• ‘•–”— –‡†—•‹‰ ‘‹ƒ’˜ͶǤͳǤ‡˜‡”ƒŽ“—ƒ–‹–ƒ–‹˜‡–”ƒ‹–Ž‘ ‹ȋȌ™‡”‡†‡–‡ –‡†ˆ‘”  ƒ†  ‘ Ͷ ƒ† ͹Ǥ  ’”‘˜‹†‡† ƒ ”ƒ’‹† ƒ† ”‡Žƒ–‹˜‡Ž› Ž‘™ ‘•– ‡–Š‘† –‘ ’”‘†— ‡ƒǦ„ƒ•‡†ƒ’‹ƒ‘˜‡Ž’”‘‰‡›Ǥ. . ͹ͻ.

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