1 Article title: Hydrogel-based delivery of Tat-fused protein Hsp70 protects dopaminergic cells in vitro and in a mouse model of Parkinson’s disease
Journal name: NPG Asia Materials
Author names: Marta Tunesi, Ilaria Raimondi, Teresa Russo, Laura Colombo, Edoardo Micotti, Edoardo Brandi, Pamela Cappelletti, Alberto Cigada, Alessandro Negro, Luigi Ambrosio, Gianluigi Forloni, Loredano Pollegioni, Antonio Gloria, Carmen Giordano and Diego Albani
Correspondence to: Prof. Carmen Giordano, Department of Chemistry, Materials and Chemical Engineering “G. Natta”, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy
Phone: +39.02.2399.3122 Fax: +39.02.2399.3280
E-mail: carmen.giordano@polimi.it
SUPPLEMENTARY INFORMATION S3 (Acrobat file) VIDEO S3 (Video avi file)
Text summary: Supplementary Information S3 provides the group description for the mice performing the beam walk test, while Video S3 shows a sample video record of one mouse/group while performing the motor task.
Supplementary Figure S3 and Video S3: Group description for the mice performing the beam walk test and video record of one mouse/group while performing the motor task
As an example, we provided a video showing the mice (one/group) performing the beam walk test. Mouse nr°2 received 5 µL PBS + 5 µL PBS + 5 µL PBS, mouse nr°36 received 5 µL PBS + 4 µg 6-OHDA + 5 µL PBS, mouse nr°11 received 5 µL (1.75 µg) free Hsp70 + 4 µg 6-OHDA + 5 µL (1.75 µg) free
Tat-2 Hsp70, while mouse nr°25 received 5 µL Tat-Hsp70-loaded composites (containing 1.75 µg Tat-Hsp70) + 4 µg 6-OHDA + 5 µL Tat-Hsp70-loaded composites (containing 1.75 µg Tat-Hsp70).
