Characterization of 25 full-length S-RNase alleles, including flanking regions, from a pool of resequenced apple cultivars
Plant Molecular Biology
Paolo De Franceschi
1, Luca Bianco, Alessandro Cestaro, Luca Dondini, Riccardo Velasco
1
Corresponding author. Dipartimento di Scienze Agrarie, Università degli Studi di Bologna, Bologna, Italy – email [email protected]
Online resource 3 Coverage and allele confirmation for three genotypes in disagreement with previous
reports; the reads coverage on selected alleles and alignment of the contig on the reference sequences are
shown for: A. cv Borowitsky, allele S28; B. cv Priscilla, allele S7; C. cv Priscilla, allele S10; D. cv Worcester
Pearmain, allele S25. Coding sequences are highlighted in yellow in each alignment. See Table 1 in the
paper for accession numbers of sequences used as reference.
A. ‘Borowitski’ allele S28 (contig of 124 reads, 1058bp)
S28-Borowitski AATTAATCTGCCTCGCTCTTGAACAAATTAATATTATTCAATGGGAATTACGGGGATGAT S28-Reference tattgatctgcctcgctcttgaacaaattaatattattcaatgggaattacggggatgat *** *******************************************************
S28-Borowitski ATATATGTTTACAATGGTATTTTCATTAATAGTATTAATATTGTCTTCGTCCACGGTGGG S28-Reference atatatgtttacaatggtattttcattaatagtattaatattgtcttcgtccacggtggg ************************************************************
S28-Borowitski ATACGATTATTTTCAATTTACGCAGCAATATCAGCCGGCCGTATGCAACTCTAATCCTAC S28-Reference atacgattattttcaatttacgcagcaatatcagccggccgtatgcaactctaatcctac ************************************************************
S28-Borowitski TCCTTGTAACGATCCTCCTGAAAAATTGTTTACGGTTCACGGTTTGTGGCCTTCAAACAA S28-Reference tccttgtaacgatcctcctgaaaaattgtttacggttcacggtttgtggccttcaaacaa ************************************************************
S28-Borowitski GAATGGACCTGACCCAGAAAAATGCAAGAATATACAAATGAATTCTCAGAAGGTAATATT S28-Reference gaatggacctgacccagaaaaatgcaagaatatacaaatgaattctcagaaggtaatatt ************************************************************
S28-Borowitski ATTAATAATGAGATAGTCAATATTGTTTATTTCATTTATGTACTTGTGTATAAAATATAT S28-Reference attaataatgagatagtcaatattgtttatttcatttatgtacttgtgtataaaatatat ************************************************************
S28-Borowitski TACATATACTCAACATAGATTTTCATGCACGCCTGTGGAAATATTACAATTAATTTAAAA S28-Reference tacatatactcaacatagattttcatgcacgcctgtggaaatattacaattaatttaaaa ************************************************************
S28-Borowitski TTTAATCATGAATTGTTTCTATTATATAATTATATTGTCAGATAGGAAATATGGCAGCCC S28-Reference tttaatcatgaattgtttctattatataattatattgtcagataggaaatatggcagccc ************************************************************
S28-Borowitski AGTTGGAAATTATTTGGCCGAACGTACTCAATCGAACTGATCATGTAGGCTTCTGGGAAA S28-Reference agttggaaattatttggccgaacgtactcaatcgaactgatcatgtaggcttctgggaaa ************************************************************
S28-Borowitski GAGAGTGGCTCAAACATGGCACCTGCGGGTATCCCACAATAAGGGACGACATGCATTACT S28-Reference gagagtggctcaaacatggcacctgcgggtatcccacaataagggacgacatgcattact ************************************************************
S28-Borowitski TAAAAACAGTAATCAAAATGTACATAACCCAGAAACAAAACGTCTCTGCAATTCTCGCAA S28-Reference taaaaacagtaatcaaaatgtacataacccagaaacaaaacgtctctgcaattctcgcaa ************************************************************
S28-Borowitski AGGCGATGATTCAACCGAACGGGCAAAACAGGTCATTGGTGGATATTGAAAATGCCATAC S28-Reference aggcgatgattcaaccgaacgggcaaaacaggtcattggtggatattgaaaatgccatac ************************************************************
S28-Borowitski GCAGTGGTACCAACAATATGAAACCAAAATTCAAGTGTCAAAAGAATACTAGGACAACGA S28-Reference gcagtggtaccaacaatatgaaaccaaaattcaagtgtcaaaagaatactaggacaacga ************************************************************
S28-Borowitski CGGAATTGGTTGAGGTCACTCTTTGCCGTGATAGAGACTTAACGAAGTTCATAAATTGCC S28-Reference cggaattggttgaggtcactctttgccgtgatagagacttaacgaagttcataaattgcc ************************************************************
S28-Borowitski CCCAACCACCACAAGGATCACGATATCTCTGCCCCGCCGATGTTCAGTATTAATTAAGAG S28-Reference cccaaccaccacaaggatcacgatatctctgccccgccgatgttcagtattaattaagag ************************************************************
S28-Borowitski CGCGGCTAGCCACAATTACACACACACACACACACATATATATATATATAGTTGGCTTAT S28-Reference cgcggctagccacaattacacacacacacacacacacatatatatatatagttggcttat ************************************ ***********************
S28-Borowitski ATACGGATGATGATTTCCATATATAGGACGAGATGAAGACACTTATGTGATTGTATTTCT S28-Reference atacggatgatgatttccatatataggacgagatgaagacacttatgtgattgtatttct ************************************************************
S28-Borowitski AATGCATAAAACCCTTTTTATCAAGGTTGGTTTCGGA S28-Reference aatgcataaaaccctttttatcaaggttggtttcgg- ************************************
B. ‘Priscilla’ allele S7 (contig of 116 reads, 932bp)
S7-Priscilla ---ATCGATCAAATTAGTAATTAATCTGCCTCGCTCTTCGACTAATAT S7-Reference ccaccaccacttcgaatcgatcaaattagtaattaatctgcctcgctcttcgactaatat *********************************************
S7-Priscilla TATTCAATGGGGATTACGGGGATGATATATATGGTTACGATAGTATTTTCATTAATTGTT S7-Reference tattcaatggggattacggggatgatatatatggttacgatagtattttcattaattgtt ************************************************************
S7-Priscilla TTACTATTGTCTTCGTCCGCGGCGAGATACGATTATTTTCAATTTACGCAGCAGTATCAG S7-Reference ttactattgtcttcgtccgcggcgagatacgattattttcaatttacgcagcagtatcag ************************************************************
S7-Priscilla TTGGCTGCCTGCAACTCTAAACCTATTCCTTGTAAGGATCCTCCTGACAAGTTGTTTACG S7-Reference ttggctgcctgcaactctaaacctattccttgtaaggatcctcctgacaagttgtttacg ************************************************************
S7-Priscilla GTTCACGGTTTGTGGCCTTCAGACTCGAATGGACATGACCCAGTAAATTGCAGTAAATCA S7-Reference gttcacggtttgtggccttcagactcgaatggacatgacccagtaaattgcagtaaatca ************************************************************
S7-Priscilla ACCGTGGATGCTCAGAAGGTAATCTTATCAATAATCTAATACTCAACATATATGTTCATA S7-Reference accgtggatgctcagaaggtaatcttatcaataatctaatactcaacatatatgttcata ************************************************************
S7-Priscilla CAAGTGCAAATATTACAATTAATTTCAATTTAATCATAAATGTTTTCTATTTATATATTT S7-Reference caagtgcaaatattacaattaatttcaatttaatcataaatgttttctatttatatattt ************************************************************
S7-Priscilla TTATTTATATTGGCAGTTAGGAAATCTGACAACTCAGTTGGAAATAATTTGGCCGAACGT S7-Reference ttatttatattggcagttaggaaatctgacaactcagttggaaataatttggccgaacgt ************************************************************
S7-Priscilla ATACAATCGAACCGATCATATAAGCTTCTGGGATAAACAGTGGAACAAACATGGCACCTG S7-Reference atacaatcgaaccgatcatataagcttctgggataaacagtggaacaaacatggcacctg ************************************************************
S7-Priscilla TGGACATCCCACAATAATGAACGACATTCATTACTTTCAAACAGTAATCAAAATGTACAT S7-Reference tggacatcccacaataatgaacgacattcattactttcaaacagtaatcaaaatgtacat ************************************************************
S7-Priscilla AACCCAGAAACAAAACGTCTCTAAAATCCTCTCAAGGGCGAAGATTGAACCGGAGGGGAA S7-Reference aacccagaaacaaaacgtctctaaaatcctctcaagggcgaagattgaaccggaggggaa ************************************************************
S7-Priscilla ACCCAGGAAACAGGTAGATATTGTAAATGCCATACGCAAAGGTACAAACGATAAGGAACC S7-Reference acccaggaaacaggtagatattgtaaatgccatacgcaaaggtacaaacgataaggaacc ************************************************************
S7-Priscilla AAAACTCAAGTGCCAAAAGAATAACCAGGTGACTGAATTGGTTGAGGTCACTCTTTGCAG S7-Reference aaaactcaagtgccaaaagaataaccaggtgactgaattggttgaggtcactctttgcag ************************************************************
S7-Priscilla TAATCGCAACCTAACGGGGTTCATAAATTGCCCCCGCCATATTCCAAATGGATCACGATA S7-Reference taatcgcaacctaacggggttcataaattgcccccgccatattccaaatggatcacgata ************************************************************
S7-Priscilla TTCCTGCCCCACCAAAAATATTCTGTATTAAGAGCGCTGCTAGCTATATATATAGACGGG S7-Reference ttcctgccccaccaaaaatattctgtattaagagcgctgctagctatatatatagacggg ************************************************************
S7-Priscilla TGACCATTTCCATATAATGAATAACTTGGCTTAATTAATTAAACTCA--- S7-Reference tgaccatttccatataatgaataacttggcttaattaattaaactcatgtgaatatg ***********************************************
C. ‘Priscilla’ allele S10 (contig of 413 reads, 2435bp)
S10-Priscilla ---GGATGATATATATGGTTACAATGGTATTTTCATTAATTGTATTAATG S10-Reference atggggattacagggatgatatatatggttacaatggtattttcattaattgtattaatg ***********************************************
S10-Priscilla TTGTCTTCGTCCGCGGTGAAATTCGATTATTTTCAATTTACGCAGCAATATCAGCCGGCT S10-Reference ttgtcttcgtccgcggtgaaattcgattattttcaatttacgcagcaatatcagccggct ************************************************************
S10-Priscilla GTCTGCAACTCTAATCCTACTCCTTGTAAGGATCCTCCGGACAAGTTGTTTACGGTTCAT S10-Reference gtctgcaactctaatcctactccttgtaaggatcctccggacaagttgtttacggttcat ************************************************************
S10-Priscilla GGTTTGTGGCCTTCAAATGTTAATGGAAGTGACCCCAAGAAATGCAAAGCTACAATCTTA S10-Reference ggtttgtggccttcaaatgttaatggaagtgaccccaagaaatgcaaagctacaatctta ************************************************************
S10-Priscilla AATCCTCAAACGGTAATATTATTCCAGAAAAATTAATATCCGGTCCTTAGTGATAATGTT S10-Reference aatcctcaaacggtaatattattccagaaaaattaatatccggtccttagtgataatgtt ************************************************************
S10-Priscilla AATTAATTGAGACCTTATTAGTTTTTAGATTTTAAGTCCCTAACATTATTGTATAAATGA S10-Reference aattaattgagaccttattagtttttagattttaagtccctaacattattgtataaatga ************************************************************
S10-Priscilla ATTCAAATGTCAGTTACATAATTTTTTCAAATAAAAATTAGTAATTGAAACTTACACCCT S10-Reference attcaaatgtcagttacataattttttcaaataaaaattagtaattgaaacttacaccct ************************************************************
S10-Priscilla TATTAAACTCCTAACTAATTTTCAATTCAAAACATTTCCAAAAAATAAAAATTAGAATAA S10-Reference tattaaactcctaactaattttcaattcaaaacatttccaaaaaataaaaattagaataa ************************************************************
S10-Priscilla GTTTGTACCCATTAATTTTTTATAAAAAGATTTTCATTTGTTTAATCTTAAATGCAGCCA S10-Reference gtttgtacccattaattttttataaaaagattttcatttgtttaatcttaaatgcagcca ************************************************************
S10-Priscilla TTCATATAATTTTTCAATTTTTTCTAATCTTACATTAACCCATTCTTAAATATACCATTT S10-Reference ttcatataatttttcaattttttctaatcttacattaacccattcttaaatataccattt ************************************************************
S10-Priscilla TCTAAAATCCGTTCAAATTTTTTTTTCTAAACCATTTTTTTTTTCTAAACCATTTATTTT S10-Reference tctaaaatccgttcaaatttttttttctaaaccattttttttttctaaaccatttatttt ************************************************************
S10-Priscilla AAATTATCCATTTAATGTAACCATTTTTTATATTAAATCCATTCAATTTTTGCAACATCC S10-Reference aaattatccatttaatgtaaccattttttatattaaatccattcaatttttgcaacatcc ************************************************************
S10-Priscilla CACATTGTCTAGGGGAGTGGATCCTCTAAGCCTTATATGTATATTCACATCTCTTCCTAG S10-Reference cacattgtctaggggagtggatcctctaagccttatatgtatattcacatctcttcctag ************************************************************
S10-Priscilla CACGAGGCATTTTGGGAGCTCACTGGCTTCGGRTTCCATCGGAACTCTGAAGTTAAGCGA S10-Reference cacgaggcattttgggagctcactggcttcggattccatcggaactctgaagttaagcga ******************************** ***************************
S10-Priscilla GTTCACGCGAGAGCAATCCCATGATGGGTGACCCACTAGGAAGTTCTCGTGTGAGTTCCC S10-Reference gttcacgcgagagcaatcccatgatgggtgacccactaggaagttctcgtgtgagttccc ************************************************************
S10-Priscilla AGAAACAAAACAGTGAGGGCGTGGTCGGGGCCCAAAGCAGACAATATCATGCTACGCTGG S10-Reference agaaacaaaacagtgagggcgtggtcggggcccaaagcagacaatatcatgctacgctgg ************************************************************
S10-Priscilla AGTCGAGCCCGGGATGTGGTGGGGGCCCGGGCAGGGATGTGACAATTTGGTATCAGAGCC S10-Reference agtcgagcccgggatgtggtgggggcccgggcagggatgtgacaatttggtatcagagcc ************************************************************
S10-Priscilla AATCCATGGCCGGATGTGTGCMGACGAGAACGTCGGGCCC--- S10-Reference aatccatggccggatgtgtgcagacgagaacgtcgggcccctaagggggtggatgtaact ********************* ******************
S10-Priscilla ---CCGGCCCGGGCCCCCACCACAT S10-Reference ctaggggagtgcatcatgtaaccctaatatgtcacatcccggcccgggcccccaccacat **********************
S10-Priscilla CCCGAACTCGACTCCGCCGTAGCACGATATTGTCCGCTTTGGGCCCCGACCACGCCCTCA S10-Reference cccgaactcgactccgccgtagcacgatattgtccgctttgggccccgaccacgccctca ************************************************************
S10-Priscilla CGGTTTTGTTTCTGGGAACTCACACGAGAACTTCCCAGTGGGTCACCCAYCATGGGATTG S10-Reference cggttttgtttctgggaactcacacgagaacttcccagtgggtcacccaccatgggattg ************************************************* **********
S10-Priscilla CTCTCGCCCGAACTCGCTTAACTTCGTAGTTTCGATGGAACCCGAAGTCAGTGAGCTCTT S10-Reference ctctcgcccgaactcgcttaacttcgtagtttcgatggaacccgaagtcagtgagctctt ************************************************************
S10-Priscilla GAAAGGCCTCGTGCTAGGTAGAGATGGGAATAGGCATATAAGGCTTACATGATGCACTCC S10-Reference gaaaggcctcgtgctaggtagagatgggaataggcatataaggcttacatgatgcactcc ************************************************************
S10-Priscilla CCTAGGCGATGTGGGATGTTACATAATACCTCTTTTTTTTTTGTTTCAAAAATGTACCCA S10-Reference cctaggcgatgtgggatgttacataatacctcttttttttttgtttcaaaaatgtaccca ************************************************************
S10-Priscilla TGTTTGGTACAATAGTTTTTTGTTAGTTTAATGAACGTACCCATGTTTTCATATATATAT S10-Reference tgtttggtacaatagttttttgttagtttaatgaacgtacccatgttttcatatatatat ************************************************************
S10-Priscilla ATATATATATTGGTACAATAGTTTTTTGTTAGTTTTAATGAACGTACCCATGTTTTCATA S10-Reference atatatatattggtacaatagttttttgttagttttaatgaacgtacccatgttttcata ************************************************************
S10-Priscilla TATATATATGTATGTATGTATGTATGTATGTATGTATAAATAATATATTGGAGAGCATAA S10-Reference tatatatatgtatgtatgtatgtatgtatgtatgtataaataatatattggagagcataa ************************************************************
S10-Priscilla TTTATTCTTTAATTATTTTAATCCCATAAATGGATTTTATTTAAAATCTCATTAATATAA S10-Reference tttattctttaattattttaatcccataaatggattttatttaaaatctcattaatataa ************************************************************
S10-Priscilla ACAACCACAAATTTTAAATTTTCATTTAAAATTATAACTTACATAAAATACATTATTATA S10-Reference acaaccacaaattttaaattttcatttaaaattataacttacataaaatacattattata ************************************************************
S10-Priscilla TAATGTCAGAAACTTCGATCAAAAACTGCAAATCAGTAAAATTTCAGTTAAAAAACATTA S10-Reference taatgtcagaaacttcgatcaaaaactgcaaatcagtaaaatttcagttaaaaaacatta ************************************************************
S10-Priscilla ATAATTAGGACCACGTCCAAAATCTCCATATTATTAATAATTAGATAGTCAATAT-TGTT S10-Reference ataattaggaccacgtccaaaatctccatattattaataattagatagtcaatatttgtt ******************************************************* ****
S10-Priscilla TATCTTTTCATATATACATATGCTCGACATAGATTTTCATCCATGAAAAATATTACTATT S10-Reference tatcttttcatatatacatatgctcgacatagattttcatccatgaaaaatattactatt ************************************************************
S10-Priscilla AATTTAAAATTTAATCATAATTTTTTTCTATTATAATATTATATTGTCAGATAACAAATC S10-Reference aatttaaaatttaatcataatttttttctattataatattatattgtcagataacaaatc ************************************************************
S10-Priscilla TTAAAGCCCAGCTGGAAATTATTTGGCCAAACGTACTCAATCGAAGGGCTCATGTACGCT S10-Reference ttaaagcccagctggaaattatttggccaaacgtactcaatcgaagggctcatgtacgct ************************************************************
S10-Priscilla TCTGGCGTAAACAGTGGCGTAAACATGGCGCCTGTGGGTACCCCACAATAGCGGACGACA S10-Reference tctggcgtaaacagtggcgtaaacatggcgcctgtgggtaccccacaatagcggacgaca ************************************************************
S10-Priscilla TGCATTACTTTAGCACAGTAATCGAAATGTACATAACCAAGAAACAAAACGTCTCTGAAA S10-Reference tgcattactttagcacagtaatcgaaatgtacataaccaagaaacaaaacgtctctgaaa ************************************************************
S10-Priscilla TCCTCTCAAAGGCGAAGATTAAACCGGAGGGGAGATTCAGGACACGGGACGACATTGTAA S10-Reference tcctctcaaaggcgaagattaaaccggaggggagattcaggacacgggacgacattgtaa ************************************************************
S10-Priscilla ATGCCATAAGCCAAAGTATCGACTATAAGAAACCAAAACTCAAGTGCAAGATCAATAATC S10-Reference atgccataagccaaagtatcgactataagaaaccaaaactcaagtgcaagatcaataatc ************************************************************
S10-Priscilla AGACAACTGAATTGGTTGAGGTCGGTCTTTGCAGCGATAACAACTTAACGCAGTTCATAA S10-Reference agacaactgaattggttgaggtcggtctttgcagcgataacaacttaacgcagttcataa ************************************************************
S10-Priscilla ATTGCCCCAACCCATTTCCTCAAGGATCACCATATTTCTGCCCCACCAATAATATTCAGT S10-Reference attgccccaacccatttcctcaaggatcaccatatttctgccccaccaataatattcagt ************************************************************
S10-Priscilla ATTAAGAGCGCAGCTAGATAGTATTGCATATACGGATGATGATTTCCATATATAGGACGA S10-Reference attaagagcgcagctagatagtattgcatatacggatgatgatttccatatataggacga ************************************************************
S10-Priscilla GAGGAATGCAGTTATGTGATTGTATTTCTAATGAATAAATTGTCTTA--- S10-Reference gaggaatgcagttatgtgattgtatttctaatgaataaattgtcttaattaatt ***********************************************
D. ‘Worcester Pearmain’ allele S25 (contig of 411 reads, 3206bp)
S25-Worcester_Pearmain AATGGGGATCATGGGGATGATATATATGGTTATGATGGTATTTTCATTAATTGTATTAAT S25-Reference -atggggatcatggggatgatatatatggttatgatggtattttcattaattgtattaat ***********************************************************
S25-Worcester_Pearmain ATTGTCTTCGTCCACTGTGGGATTCGATTATTTTCAATTTACGCATCAATATCAGCCGGC S25-Reference attgtcttcgtccactgtgggattcgattattttcaatttacgcatcaatatcagccggc ************************************************************
S25-Worcester_Pearmain TGTCTGCAACTCTAATCGTACTCCTTGTAAGGATCCTCCTGACAAATTGTTTACGGTTCA S25-Reference tgtctgcaactctaatcgtactccttgtaaggatcctcctgacaaattgtttacggttca ************************************************************
S25-Worcester_Pearmain CGGTTTGTGGCCTTCAAACAGGAATGGACCTGACCCAGAGTATTGCAAGAACACAACCTT S25-Reference cggtttgtggccttcaaacaggaatggacctgacccagagtattgcaagaacacaacctt ************************************************************
S25-Worcester_Pearmain GGATGTTACCAAGGTAATATTATTAGTAATTAGACAATATTGTTTATTTCATATATACAT S25-Reference ggatgttaccaaggtaatattattagtaattagacaatattgtttatttcatatatacat ************************************************************
S25-Worcester_Pearmain ATACTCAACATAGATTTTCATACACGTGTGTGAGAATATTACAATAAATTTAAAATTTAG S25-Reference atactcaacatagattttcatacacgtgtgtgagaatattacaataaatttaaaatttag ************************************************************
S25-Worcester_Pearmain TCATAATTTTTTTCTATTGAAAATATGACTATACCCCTTGAAATTCAATTATTTTTAATT S25-Reference tcataatttttttctattgaaaatatgactataccccttgaaattcaattatttttaatt ************************************************************
S25-Worcester_Pearmain AAGAAAATTTTAACGAAAAACTTCTGATATTGTTCACTTTAACGAAAAACCACATTTTTA S25-Reference aagaaaattttaacgaaaaacttctgatattgttcactttaacgaaaaaccacattttta ************************************************************
S25-Worcester_Pearmain CACTAAATAGTCAATCCTAGTATTATATACTTTACCCTTTATTTTGTCTTTTTTGTTAAA S25-Reference cactaaatagtcaatcctagtattatatactttaccctttattttgtcttttttgttaaa ************************************************************
S25-Worcester_Pearmain ACTCAAAGTTTTTCAGCCATTTTCATTAGTTTTCCTTTTAATTAAACCCCACTATGATAA S25-Reference actcaaagtttttcagccattttcattagttttccttttaattaaaccccactatgataa ************************************************************
S25-Worcester_Pearmain ATTTACCTGACAAATACCAAATGACTAGGCTCCAACTAAGCAATATCCATAATTAAGTTT S25-Reference atttacctgacaaataccaaatgactaggctccaactaagcaatatccataattaagttt ************************************************************
S25-Worcester_Pearmain GACTTGGCAAAATTTGTTGGATTTCTAAATTACCCCCAGTACATTGACCTTGGGTCTGTT S25-Reference gacttggcaaaatttgttggatttctaaattacccccagtacattgaccttgggtctgtt ************************************************************
S25-Worcester_Pearmain ACTTGTTTTGTCTACACTAAAGGAGAGCATGTAGACTTAGTCTCACAATGCACTAGCAAT S25-Reference acttgttttgtctacactaaaggagagcatgtagacttagtctcacaatgcactagcaat ************************************************************
S25-Worcester_Pearmain AATATGGTTCAAATTTGCCTTTTGCGAGAACTGAACCTAAGTCTCTCTCTTACAAGTGAA S25-Reference aatatggttcaaatttgccttttgcgagaactgaacctaagtctctctcttacaagtgaa ************************************************************
S25-Worcester_Pearmain AAGGAATATCACTAGACTGGAGTACTAAGTGGTGCGCTCGTTACTTGTTATCTACATTTC S25-Reference aaggaatatcactagactggagtactaagtggtgcgctcgttacttgttatctacatttc ************************************************************
S25-Worcester_Pearmain TTTTACATTATTTCAATCAATTAAATGTTTTTCAAACAACTTATGAGGATTTAGAAAGAA S25-Reference ttttacattatttcaatcaattaaatgtttttcaaacaacttatgaggatttagaaagaa ************************************************************
S25-Worcester_Pearmain AAAAAATTAAATGCCATGATTTCTATGCATATTAATAACAAAAATTACATGATATTAGAG S25-Reference aaaaaattaaatgccatgatttctatgcatattaataacaaaaattacatgatattagag ************************************************************
S25-Worcester_Pearmain TTTTATTCATCATATAGTTGTTTTCACATTTGCGTCAAATTGAGCATTTTTTGTTCCTTT S25-Reference ttttattcatcatatagttgttttcacatttgcgtcaaattgagcattttttgttccttt ************************************************************
S25-Worcester_Pearmain TCAGATGATGGTAACATGTAAATTAGTAGATTCTAAAAATATAACAATGTTATTTTATTC S25-Reference tcagatgatggtaacatgtaaattagtagattctaaaaatataacaatgttattttattc ************************************************************
S25-Worcester_Pearmain AAAATACCAATATTTTACATATAAATATAGATAAATTATCTTGTACAACATATATATATA S25-Reference aaaataccaatattttacatataaatatagataaattatcttgtacaacatatatatata ************************************************************
S25-Worcester_Pearmain TATATAATAATAATAATAATAACAATATAAGTTCCTAGTAAATTATGTAATAATACATGC S25-Reference tatataataataataataataacaatataagttcctagtaaattatgtaataatacatgc ************************************************************
S25-Worcester_Pearmain CAAAATAATTTACATACTATTTAAATAAAAATTGTTTTATTCAAAATAATTAAGTACCTA S25-Reference caaaataatttacatactatttaaataaaaattgttttattcaaaataattaagtaccta ************************************************************
S25-Worcester_Pearmain TATTTTGCAAAAAAAAGAGGGTAAATTGTAACATCTCACATTGTCCAGGGGAGTGGATCT S25-Reference tattttgcaaaaaaaagagggtaaattgtaacatctcacattgtccaggggagtggatct ************************************************************
S25-Worcester_Pearmain TGTAAGCCTTATATGTATATTCTCATCTCTACCTAACACGAGGCCTTTTGRGAGCTCACT S25-Reference tgtaagccttatatgtatattctcatctctacctaacacgaggccttttgagagctcact ************************************************** *********
S25-Worcester_Pearmain GGCTTCGGGTTCCATCGGAACTCCGAAGTTAAGCGAGTTCGCGCAAGAGCAATCTCATGA S25-Reference ggcttcgggttccatcggaactccgaagttaagcgagttcgcgcaagagcaatctcatga ************************************************************
S25-Worcester_Pearmain TGGGTGACCTACTGGGAAGTTCTCCTGTGAGTTCCCAGAAACAAAACCGTGGGGGCGTGG S25-Reference tgggtgacctactgggaagttctcctgtgagttcccagaaacaaaaccgtgggggcgtgg ************************************************************
S25-Worcester_Pearmain TCATGCCnnnnnnnnnnnATATCGTGCTACGGTGGAGTCGAGCTTAGGATGTGGTGGGGG S25-Reference tcatgcccaaagcagacaatatcgtgctacggtggagtcgagcttaggatgtggtggggg ******* ******************************************
S25-Worcester_Pearmain CTCGAGCCAGGATGTGACAATTTAGTATCATAGCCAATCCCTGACCGGAAGTGTGCCGAC S25-Reference ctcgagccaggatgtgacaatttagtatcatagccaatccctgaccggaagtgtgccgac ************************************************************
S25-Worcester_Pearmain GAGGACGTCGGGCCCCTAAGGGGGGTGGATTGTAACATCCCACATCGCCCAAGGGAGTGG S25-Reference gaggacgtcgggcccctaaggggggtggattgtaacatcccacatcgcccaagggagtgg ************************************************************
S25-Worcester_Pearmain ATCTTGTAAGCCTTATATGTATATTCTCATCTCTACCTACCACGAGGCTTTTTGGGAGCT S25-Reference atcttgtaagccttatatgtatattctcatctctacctaccacgaggctttttgggagct ************************************************************
S25-Worcester_Pearmain CATTGGCTTCGAGTTCCATCGGAACTCTAAAGTTAAGCGAGTTCGCGCTAGAGCAATCCC S25-Reference cattggcttcgagttccatcggaactctaaagttaagcgagttcgcgctagagcaatccc ************************************************************
S25-Worcester_Pearmain ATGATGGGTGACCCACTGTGAAGTTCTGTTGTGAGTTCCTATAAACAAAACCGTGAGGGC S25-Reference atgatgggtgacccactgtgaagttctgttgtgagttcctataaacaaaaccgtgagggc ************************************************************
S25-Worcester_Pearmain ATGGTCAGGGCCCAAAGCGGACAATATTATGCTACGGCGGAGTCGAGCCCGGGATGTGGT S25-Reference atggtcagggcccaaagtggacaatattatgctacggcggagtcgagcccgggatgtggt ***************** ******************************************
S25-Worcester_Pearmain GGGGGTCTGGGTCGAGATGTGACATAAATTATTTGCACAAGCATATATACATATATATTA S25-Reference gggggtctgggtcgagatgtgacataaattatttgcacaagcatatatacatatatatta ************************************************************
S25-Worcester_Pearmain ATAATATTAAAATGTGCCTAACATATGTAATAATACATGCAAAATAAATAAGTGTCGATT S25-Reference ataatattaaaatgtgcctaacatatgtaataatacatgcaaaataaataagtgtcgatt ************************************************************
S25-Worcester_Pearmain GATTCCACACACCATATTATAATAACACAATGCGCCTGGTATATGTAATAATACATGCAA S25-Reference gattccacacaccatattataataacacaatgcgcctggtatatgtaataatacatgcaa ************************************************************
S25-Worcester_Pearmain AACAATTTACCTATAAAATAAATAATGTTTTTAGCCATTTGATTCAAAAAATAATTATTT S25-Reference aacaatttacctataaaataaataatgtttttagccatttgattcaaaaaataattattt ************************************************************
S25-Worcester_Pearmain AGCTATATAATAATAACAAAATGTGCATAATATGTACATGCAAAATAATTTATCTCCAAT S25-Reference agctatataataataacaaaatgtgcataatatgtacatgcaaaataatttatctccaat ************************************************************
S25-Worcester_Pearmain GGTTATCAAACAAATAAAAGAAATCGAACATATGATGGTAAGACAAATAGACACTTATGT S25-Reference ggttatcaaacaaataaaagaaatcgaacatatgatggtaagacaaatagacacttatgt ************************************************************
S25-Worcester_Pearmain AATAGTAGGGCACTTATTTTCCATAATTATTGTGGGTAAGTAAATTCATTAACATAATTA S25-Reference aatagtagggcacttattttccataattattgtgggtaagtaaattcattaacataatta ************************************************************
S25-Worcester_Pearmain GGGTGTTGTTGCATGTGTGAAAATATTTTGAAAATAATAAGTATTAATTTGTTTACATGG S25-Reference gggtgttgttgcatgtgtgaaaatattttgaaaataataagtattaatttgtttacatgg ************************************************************
S25-Worcester_Pearmain CAATCTATTTCAAATTTTGGTTCTATTTGGATATTTAAAATCATAGAGG-CTTTTGTTAT S25-Reference caatctatttcaaattttggttctatttggatatttaaaatcatagagggcttttgttat ************************************************* **********
S25-Worcester_Pearmain GAAAATAATAGTTGTACGGGCTTACATCTAAAGATTTTTTCGAATATTTTGTTCAAAAAA S25-Reference gaaaataatagttgtacgggcttacatctaaagattttttcgaatattttgttcaaaaaa ************************************************************
S25-Worcester_Pearmain AAGATTTTTTTTTCTAATATATAATATTGTCAGATAGGAAATCTCCAAGCACAGTTGGAC S25-Reference aagatttttttttctaatatataatattgtcagataggaaatctccaagcacagttggac ************************************************************
S25-Worcester_Pearmain ATTATTTGGCCGAACGTATACGATCGAACTAATAATGTAGGCTTTTGGAGTAAACAGTGG S25-Reference attatttggccgaacgtatacgatcgaactaataatgtaggcttttggagtaaacagtgg ************************************************************
S25-Worcester_Pearmain GCAAAACATGGCATCTGTGGGTCTCCCACAATACAGGACGACGTGAATTACCTTGAAACA S25-Reference gcaaaacatggcatctgtgggtctcccacaatacaggacgacgtgaattaccttgaaaca ************************************************************
S25-Worcester_Pearmain GTAATCAACTTGTACATAATCAAGAAACAAAACGTCTTTGAAATCCTGTCAAATGCCAAG S25-Reference gtaatcaacttgtacataatcaagaaacaaaacgtctttgaaatcctgtcaaatgccaag ************************************************************
S25-Worcester_Pearmain ATCGAGCCGGAGGGAAAAAACAGGACACGAAAGGACATTGTAAAAGCCATACGCAGTGGC S25-Reference atcgagccggagggaaaaaacaggacacgaaaggacattgtaaaagccatacgcagtggc ************************************************************
S25-Worcester_Pearmain ACCAAAGGTAAGAGACCAAAACTTAAGTGCCAGAAGAATAATAGGACGACTGAATTGGTT S25-Reference accaaaggtaagagaccaaaacttaagtgccagaagaataataggacgactgaattggtt ************************************************************
S25-Worcester_Pearmain GAGGTCACTCTTTGCAGCGATAGAAACTTAACGCAGTTAATAAATTGCCCCAACCTAATT S25-Reference gaggtcactctttgcagcgatagaaacttaacgcagttaataaattgccccaacctaatt ************************************************************
S25-Worcester_Pearmain AAACCAAAATCACCATATTTCTGTCCCTTGAAAAGTATTCAGTATTAAGAGCGCGGCTAG S25-Reference aaaccaaaatcaccatatttctgtcccttgaaaagtattcagtattaagagcgcggctag ************************************************************
S25-Worcester_Pearmain CTATTGTGTGTGCTCGTAAATGTAAATATATATATATATATATATATATATATATATATA S25-Reference ctattgtgtgtgctcgtaaatgtaaatatatatatatatatatatatatatatatatata ************************************************************
S25-Worcester_Pearmain TGAAGTAGTTGGCCTATATAGGGATGACGATTTCCATATATCGGACGAAAGGAATGCAAT S25-Reference tgaagtagttggcctatatagggatgacgatttccatatatcggacgaaaggaatgcaat ************************************************************
S25-Worcester_Pearmain TATGTGATTGTTTTCTAATGAATAAATTGGCTTAGTTA--- S25-Reference tatgtgattgttttctaatgaataaattggcttagttaatt **************************************
