Animal Health and Veterinary Laboratories Agency (AHVLA) International Conference 2011
STUDY ON YERSINIOSIS BY Y.pseudotuberculosis IN WILD AND DOMESTIC ANIMALS FROM ITALY
Quest'opera è stata rilasciata sotto la licenza Creative Commons Attribuzione-Non commerciale-Non opere derivate 2.5 Italia.
Per leggere una copia della licenza visita il sito web http://creativecommons.org/licenses/by-nc-nd/2.5/it/ o spedisci una lettera a Creative Commons, 171 Second Street, Suite 300, San Francisco, California, 94105, USA.
Stampato a cura dell'Unità Operativa di Supporto Biblioteca, Informazione, Editoria (2011).
* Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Perugia, Italy
** 2 Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, sez. Pavia, Italy.
** 3 Istituto Zooprofilattico sperimentale della Sardegna, Italy.
*** 4 IstitutoSuperiore di Sanità, Italy.
C.F. Magistrali*, S. Farneti*, L. Cucco*, S. Lollai**, M. Fabbi*** , L. Ercoli*, R. Ortenzi*, A.M. Dionisi ****.
Introduction
Y. pseudotuberculosis infection has been reported in various animals and in man: wild animals are considered as a reservoir but the epidemiology of this
infection is not yet fully understood.
Pulsed Field Gel Electrophoresis (PFGE) has been shown as a suitable method for subtyping Y. pseudotuberculosis strains: aim of the present work was the
characterization of isolates from clinical cases of Yersiniosis in wild and domestic animals to improve the knowledge of epidemiological features of this
infection.
Materials and methods
Forty-four isolates of Y. pseudotuberculosis, from cases of Yersiniosis (n 42) and from asymptomatic wild boars (n 2), were included in this study. The cases occurred from 1996 to 2011, in hares (28), sheep
(10), eastern cottontail (1), roe (1), canary (1) and cat (1), from 6 different Italian regions; three isolates from sheep and two from hares
respectively, derived from the same two outbreaks. All isolates were confirmed as inv - positive by PCR. PFGE was carried out, after digestion with NotI enzyme and cluster analysis was performed by
Bionumerics software.
y.pseudotubercolosis_50.1000Kb
100
95
90
85
80
75
70
65
60
55
50
45
40
35
y.pseudotubercolosis_50.1000Kb
A2536 R1848 R2590 R 2986 R 2991 R 2983 R 2984 R 2985 R 2990 R 3001 R2896 R2897 R2534 R2535 R2070 R1852 R 2987 R 3000 R 2989 R2982 R1926 R 2988 R1621 R 2998 R 2995 R2894 R2890 5939/?
R2893 R2892 58163 61394.
R2163 R1528 R2533 R 2994 R 2996 R 2997 R1599 R2532 R2550 R2889 R2900 R2888 marker
hare (Umbria) cat, septicemia (Umbria) hare (Umbria) sheep, mastitis (Sardinia) hare (Umbria) sheep, abortion (Sardinia) sheep, abortion (Sardinia) sheep abortion (Sardinia) roe, septicemia (Lombardy) hare (Emilia Romagna) hare (Lombardy) hare (Lombardy) hare (Umbria) hare (Umbria) hare (Umbria) eastern cottontail (Umbria) sheep, abortion (Sardinia) hare (Lombardy) sheep, abortion (Sardinia) sheep, mastitis (Sardinia) hare (Umbria) sheep,abortion (Sardinia) hare (Umbria) hare (Veneto) hare (Emilia Romagna) hare (Lombardy) hare (Lombardy) hare (Lombardy) hare (Lombardy) hare (Lombardy) wild boar, asymptomatic (Umbria) wild boar, asymptomatic (Umbria) hare(Umbria) hare (Umbria) sheep, abortion (Lazio) hare (Lombardy) hare (Emilia Romagna) hare(Emilia Romagna) hare (Umbria) sheep, abortion (Lazio) canary septicemia (Marche) hare (Lombardy) hare (Lombardy) Hare (Lombardy)
2009 2007 2010 2005 2010 2000 2000 2000 2009 2001 1996
* 2009 2009 2008 2007 2001 2011 2004 2010 2007 2004 2007 2011 2010 1996 1996
* 2003
* 2009 2009 2008 2007 2009 2010 2010 2011 2007 2009 2009
* 1996 1996
Results and Discussion
The obtained dendrogram described 40 different PFGE patterns, with a similarity coefficient ranging from 65% to 100% and grouped isolates from the same outbreak with a genetic similarity >95%; nevertheless, the same similarity was observed between two isolates from hares from Emilia Romagna (2010-2011), and between isolates from sheep and from hares,
differing for region and year of isolation. Eight different groups were described with 85% similarity, including isolates from different areas,
species and years but belonging to the same group. The study of Yersiniosis in animals can improve the knowledge of epidemiological features of this infection, necessary to prevent effectively outbreaks of
Yersiniosis in animals and man.
Fig. 1: dendrogram of Y.pseudotuberculosis isolates after PFGE (NotI): species, year and Region of origin are also indicated
Isolated in the same outbreak
Different species, Region and year of origin Different year of origin
Fig. 2: Lesions in kidney, spleen and lung from cases in hares (1,2,3); after histological examination, foci of necrosis were detected in a lung from an ovine
aborted fetus (4) and in a liver from a cat (5).
References
•Isberg R.R., Voorhis D.L., Falkow S. 1987. Identification of invasion: a protein that allows enteric bacteria to penetrate cultured mammalian cells. Cell. 50, 769-778
•Stenkova A.M., Isaeva M. P. and Rasskavoz V. A. (2008). Development of a multiplex PCR procedure for detection of Yersinia genus with identification of pathogenic species (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica). Molecular Genetics,
Microbiology and Virology, 23, 119-125.
•Thoerner P., Bin Kingombe C.I., Bögli- Stuber K., Bissig-Choisat B.,Wassenaar T.M., Frey J., Jemmi T. 2003. PCR detection of virulence genes in Yersinia enterocolitica and Yersinia pseudotuberculosis and investigation of virulence gene distribution.
Applied and Environmental Microbiology 69, 1810-1816
Isolated in the same outbreak
Different species, Region and year of origin
