Proceedings of the Joint Congress SIBV-SIGA Milano, Italy – 8/11 September, 2015
ISBN 978-88-904570-5-0
Oral Communication Abstract – B.03
REDUCING SUSCEPTIBILITY TO POWDERY MILDEW IN APPLE AND
GRAPEVINE THROUGH SILENCING OF MLO GENES
PESSINA S.*, CAMPA M.*,**, DALLA COSTA L.*, PERAZZOLLI M.*, LENZI L.*, VALÈ
G.***, URSO S.***, SCHOUTEN H.****, VISSER R.****, SALAMINI F.*, VELASCO R.*, MALNOY M.*
*) Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige (I) **) Department of Genetics, University of Stellenbosch (NZ)
***) CRA-RIS, Rice Research Unit, Vercelli (I)
****) Wageningen UR Plant Breeding, Wageningen University and Research Centre (NL)
Powdery mildew, grape, resistance gene, MLO, gene silencing
Powdery mildew (PM) is a major fungal disease for apple and grape that requires a huge amount of chemicals to be controlled. Specific homologs of the MLO gene family act as PM-susceptibility genes, since their loss-of-function mutations ended up in durable and broad-spectrum resistance in several crops. PM pathogenesis is linked to a pathogen-dependent up-regulation of specific members of the MLO gene family during early stages of infection and, since they are negative regulator of plant defense pathways, their up-regulation impair the defenses of the host. The silencing or knock-out of up-regulated MLO genes led to resistance in Arabidopsis, tomato, pea, pepper, barley and wheat. Pleiotropic phenotypes are sometimes associated to mlo mutants, like early senescence-like leaf chlorosis under non-optimal growth condition. Using RNAi, four grape MLO genes and two apple MLO genes were silenced to obtain resistance respectively to Erysiphe necator (grape) and Podosphaera leucotricha (apple). Grape and apple transgenic lines have been tested for resistance to PM causal agent, showing variable levels of resistance, from moderate to very high, without deleterious phenotypic effects. The most resistant grape and apple transgenic lines have been selected for further characterization, with a particular focus on gene expression and microscopy.
