33rd International Specialised Symposium on Yeasts
Exploring and Engineering
Yeasts for Industrial
Application
26 – 29 June 2017
University College Cork, Ireland
http://microb.io/ISSY33
#ISSY33
FOCUSED MEETING
2017
POSTER ABSTRACT
BOOK
1 | Abstracts are published as received from the authors and are not subject to editing
Contents:
Session
Poster number
Page number
Genomics and Hybrids
P1 – P18
2 - 19
Interconnected Metabolic and Stress Networks
P19 – P30
20 - 31
Strategies for Engineering Yeast Genomes
P31 – P38
32 - 39
Tolerating the Stress of Industrial Processes
P39 – P48
40 - 50
Exploiting Evolution to Develop Optimised Strains
P49 – P57
51 - 59
Diverse Yeast Exploitation
P58 – P92
60 - 94
Engineering Yeast Cell Factories 1
P93 – P103
95 - 105
Engineering Yeast Cell Factories 2
P104 – P113
106 – 115
Yeast biodiversity contributions to fermented beverages
P114 – P148
116 - 150
90 | Abstracts are published as received from the authors and are not subject to editing
Diverse Yeast Exploitation P88
Effect of pH and salinity on biofilm-like phenotypes of yeasts isolated from fermented olives Rossana Sidari, Alessandra Martorana, Andrea Caridi
Department AGRARIA, Mediterranea University of Reggio Calabria, Reggio Calabria, Calabria, Italy
The yeast biofilm-like phenotypes such as media invasiveness and mat colony are considered mechanisms to colonize and persist in environmental niches. In table olive fermentation, pH and salinity are two hurdles that yeasts have to overcome to succeed in developing a stable population. Eight yeast strains - among which Candida, Pichia, and Wickerhamomyces genera - isolated from table olive fermentations, and the two control strains of Saccharomyces cerevisiae ∑1278b and BY4742 were tested in YPD agar either carbon-rich (glucose 2%) or -deficient (glucose 0.1%) media also modified both for pH (4.3) and salinity (NaCl 5%) to simulate the brine. Conventional (2%) and low (0.3%) agar concentrations were used to study invasiveness and mat colony formation, respectively. The majority of the strains showed bigger mat colonies with 2% of glucose than 0.1%. Three strains exhibited an increase in area colonies growing in the modified media. The pH and salinity modifications determined invasive growth for six and two strains in the presence of either 2% or 0.1% of glucose, respectively. Two strains were unable to invade media. A wide diversity was observed among the strains and media; moreover, some of the strains displayed the two biofilm-like phenotypes in dissociated way. The observed phenotypic diversity could confer strain advantage during the olive fermentation process. Our results may be taking into account to select strains to drive fermentation process.
This work was supported by PON03PE_00090-2 – Modelli sostenibili e nuove tecnologie per la valorizzazione delle olive e dell’olio extra-vergine di oliva prodotto in Calabria.
