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Candidate gene functional profiling during fruit development and ripening in apple

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6thRosaceousGenomicsConference

Mezzocorona,Italy–30thSeptemberͲ04thOctober2012    ͺ͹   Highorlowfructose?Consequencesforsugarmetabolisminpeachfruit  Desnoues,E. (1)ǡ‹„‘ǡǤ (2)ǡƒŽ†ƒœœ‹ǡǤ (3)ǡ‹‰‘”‡–ǡǤ (1)ǡ±ƒ”†ǡǤ (3)ǡ—‹Ž‘–Ǧ—”‹‘ǡ Ǥ(1)  (1) ǡͳͲͷʹ±±–‹“—‡‡–±Ž‹‘”ƒ–‹‘†‡• ”—‹–•‡–±‰—‡• ǦͺͶͳͶ͵‘–ˆƒ˜‡–ǡ ”ƒ…‡ (2) ǡͳ͵͵ʹ ”—‹–‹‘Ž‘‰›ƒ†ƒ–Š‘Ž‘‰› Ȃ͵͵ͺͺ͵‹ŽŽ‡ƒ˜‡†ǯ”‘ǡ ”ƒ…‡ (3) ǡͳͳͳͷŽƒ–‡•‡–›•–°‡•†‡…—Ž–—”‡ ‘”–‹…‘Ž‡• ǦͺͶͻͳͶ˜‹‰‘ǡ ”ƒ…‡  ”—‹––ƒ•–‡‹•Žƒ”‰‡Ž›ƒˆˆ‡…–‡†„›–Š‡…‘–‡–‘ˆ•—‰ƒ”•ƒ†ƒ…‹†•Ǥ ”—…–‘•‡‹•–Š‡•™‡‡–‡•– –ƒ•–‹‰•—‰ƒ”Ǥ …‘‡”…‹ƒŽ’‡ƒ…Šˆ”—‹–ǡ•—…”‘•‡‹•–Š‡ƒ‹•—‰ƒ”ǡˆ‘ŽŽ‘™‡†„›ˆ”—…–‘•‡ ƒ†‰Ž—…‘•‡™Š‹…ŠŠƒ˜‡•‹‹Žƒ”Ž‡˜‡Ž•Ǥ –‡”‡•–‹‰Ž›Ž‘™ˆ”—…–‘•‡ƒ……‡••‹‘•Šƒ˜‡„‡‡ †‡•…”‹„‡†‹™‹Ž†’‡ƒ…Š‡•Ǥ™‘Š›’‘–Š‡•‡•…ƒ‡š’Žƒ‹–Š‹•ˆ”—…–‘•‡†‡ˆ‹…‹‡…›ǣ”‡†—…‡† •›–Š‡•‹•‘”‹…”‡ƒ•‡††‡‰”ƒ†ƒ–‹‘ǤŠ”‘—‰Šƒ‡š–‡•‹˜‡’”‘ˆ‹Ž‹‰‘ˆ‡–ƒ„‘Ž‹–‡•ƒ† ‡œ›ƒ–‹…ƒ…–‹˜‹–‹‡•ǡ–Š‹••–—†›ƒ‹•ƒ–‹Ȍ†‡•…”‹„‹‰•—‰ƒ”‡–ƒ„‘Ž‹•‹’‡ƒ…Šˆ”—‹–ƒ– †‹ˆˆ‡”‡– †‡˜‡Ž‘’‡–ƒŽ •–ƒ‰‡• ƒ† ‹‹Ȍ …‘’ƒ”‹‰ –™‘ ‰‡‘–›’‡• ™‹–Š …‘–”ƒ•–‡† ˆ”—…–‘•‡Ȁ‰Ž—…‘•‡ ”ƒ–‹‘•Ǥ ‡ Šƒ˜‡ ‡ƒ•—”‡† ͳʹ ‡–ƒ„‘Ž‹–‡• ƒ† ͳʹ ‡œ›‡ ƒ…–‹˜‹–‹‡• †—”‹‰ˆ”—‹–‰”‘™–Šǡˆ‘”–™‘‰‡‘–›’‡•‘˜‡”–™‘›‡ƒ”•Ǥ‡‘–›’‹…ƒ†›‡ƒ”‡ˆˆ‡…–•™‡”‡ ‘„•‡”˜‡† ˆ‘” •‘‡ ‡–ƒ„‘Ž‹–‡•ǡ ™Š‡”‡ƒ• –Š‡ ‡œ›‡ ƒ…–‹˜‹–‹‡• ™‡”‡ •–ƒ„Ž‡ „‡–™‡‡ ‰‡‘–›’‡•ƒ†›‡ƒ”•Ǥ‘”‡•’‡…‹ˆ‹…ƒŽŽ›ǡ™‡†‹†‘–†‡–‡…–ƒ›†‹ˆˆ‡”‡…‡‹–Š‡ƒ…–‹˜‹–‹‡• ‘ˆ–Š‡‡œ›‡•”‡•’‘•‹„Ž‡ˆ‘”•›–Š‡•‹•‘”†‡‰”ƒ†ƒ–‹‘‘ˆˆ”—…–‘•‡Ǥ ‹ƒŽŽ›ǡ‘—””‡•—Ž–• •Š‘™ƒŠ‹‰ŠŽ›”‡‰—Žƒ–‡†•›•–‡‹™Š‹…ŠƒƒŒ‘”’‡”–—”„ƒ–‹‘‹ƒ…‡–”ƒŽ…‘’‘—† Šƒ• ‘Ž› •Ž‹‰Š– ”‡’‡”…—••‹‘• ‘ •—‰ƒ” ‡–ƒ„‘Ž‹•Ǥ —”–Š‡” ‡š’Žƒƒ–‹‘• ˆ‘” –Š‡ Ž‘™ ˆ”—…–‘•‡ ’Š‡‘–›’‡ ƒ”‡ †‹•…—••‡†ǡ •—…Š ƒ• †‹ˆˆ‡”‡– •—„•–”ƒ–‡ ƒˆˆ‹‹–‹‡• „‡–™‡‡ ‹•‘Ǧ ‡œ›‡•ǡ Ž‹‹–‡† ˆ”—…–‘•‡ •–‘”ƒ‰‡ ”‡•—Ž–‹‰ ‹ Š‹‰Š‡” †‡‰”ƒ†ƒ–‹‘ ‘” ƒ †‹ˆˆ‡”‡–‹ƒŽ …‘•—’–‹‘‘ˆ–Š‡–™‘Š‡š‘•‡•ˆ‘””‡•’‹”ƒ–‹‘ǡ…‡ŽŽ™ƒŽŽ‘”•›–Š‡•‹•‘ˆ‘–Š‡”…ƒ”„‘ …‘’‘—†•Ǥ 

Candidate gene functional profiling during fruit development and ripening in apple  S.Longhi(1Ȍǡƒ†‹‡ŽŽ‘Ǥ(1Ȍǡ…Š‘Ž–œǤ(1Ȍǡ‡Š”‡•Ǥ(1Ȍǡ ”ƒ…‡•…Š‹Ǥ(1Ȍǡ‹ƒ•‹‘Ž‹ Ǥ(1Ȍǡ ‡Žƒ•…‘Ǥ(1Ȍǡ‘‘‹Ǥ(2Ȍǡ ‡””ƒ”‹‹Ǥ(2Ȍǡ‡ŽŽ‡†‘‡Ǥ(2Ȍǡ‘•–ƒ Ǥ(1Ȍ  ȋͳȌ‡•‡ƒ”…Šƒ† ‘˜ƒ–‹‘‡–”‡ǡ ‘†ƒœ‹‘‡†—†ƒ…Šǡƒ‹…Š‡Ž‡ƒŽŽǯ†‹‰‡ ȋ”‡–‘Ȍǡ –ƒŽ› ȋʹȌ‡’ƒ”–‡–‘ˆ‹‘–‡…Š‘Ž‘‰›ǡ‹˜‡”•‹–›‘ˆ‡”‘ƒǡ –ƒŽ›  Š‡’Š›•‹‘Ž‘‰‹…ƒŽ’”‘…‡••‘ˆˆ”—‹–†‡˜‡Ž‘’‡–ƒ†”‹’‡‹‰‹•–Š‡”‡•—Ž–‘ˆƒˆ—…–‹‘ƒŽ ‰‡‡‹–‡”’Žƒ›‰‘˜‡”‹‰–Š‡ˆ‘”ƒ–‹‘‘ˆ–Š‡ˆ‡ƒ–—”‡•…Šƒ”ƒ…–‡”‹œ‹‰–Š‡ˆ”—‹–“—ƒŽ‹–› ’”‘’‡”–‹‡•Ǥ ‘‰ –Š‡ •‡˜‡”ƒŽ ’Š›•‹‘Ž‘‰‹…ƒŽ ’”‘…‡••‡•ǡ –Š‡ ‘†‹ˆ‹…ƒ–‹‘• ‘……—””‹‰ ‹ –Š‡…‡ŽŽ™ƒŽŽƒ”‡–Š‡‘•–”‡Ž‡˜ƒ–ƒ†‹’‘”–ƒ–ǡˆ‘”–Š‡‹”‹’ƒ…–‹–Š‡ƒ‹–‡ƒ…‡‘ˆ –Š‡‰‡‡”ƒŽˆ”—‹–“—ƒŽ‹–›ƒ•™‡ŽŽƒ•–Š‡ƒƒ‰‡‡–‘ˆ–Š‡’‘•–Šƒ”˜‡•–•–‘”ƒ‰‡Ǥ‡•‹†‡

(2)

6thRosaceousGenomicsConference

Mezzocorona,Italy–30thSeptemberͲ04thOctober2012    ͺͺ  –Š‡•’‡…‹ˆ‹…‰‡‡ƒ…–‹˜ƒ–‹‘‘˜‡”–Š‡ˆ”—‹–…›…Ž‡ǡƒŽ•‘–Š‡’ƒ”–‹…—Žƒ”‰‡‡–‹……‘•–‹–—–‹‘ …ƒ’Žƒ›‹’‘”–ƒ–…‘–”‘ŽŽ‹‰”‘Ž‡•Ǥ ‘ ‹˜‡•–‹‰ƒ–‡ –Š‡ ˆ—…–‹‘ƒŽ ƒ…Š‹‡”› ‹˜‘Ž˜‡† ‹ –Š‡ …‘–”‘Ž ‘ˆ ˆ”—‹– –‡š–—”‡ ƒ† ‡–Š›Ž‡‡ ’”‘†—…–‹‘ †—”‹‰ ˆ”—‹– ”‹’‡‹‰ǡ –™‘ †‹ˆˆ‡”‡– …—Ž–‹˜ƒ”•ǡ •—…Š ƒ• ‘Ž†‡ ‡Ž‹…‹‘—•ƒ†”ƒ›‹–Šǡ™‡”‡•‡Ž‡…–‡†ƒ†ƒ•’‡…‹ˆ‹…•‡–‘ˆ•ƒ’Ž‡•™‡”‡…‘ŽŽ‡…–‡†ǡ ’”‘…‡••‡† ƒ† Š›„”‹†‹œ‡† ‘˜‡” ƒ ƒ† Š‘… †‡•‹‰‡† …—•–‘ ‹…”‘ƒ””ƒ› ’Žƒ–ˆ‘”Ǥ Š‡ •’‡…‹ˆ‹… ”‡‰—Žƒ–‹‘ ‘ˆ ƒ’’”‘š‹ƒ–‡Ž› ͵ͺͲͲ ‰‡‡• ‹˜‘Ž˜‡† ‹ ˆ”—‹– ”‹’‡‹‰ ƒ† ”‡‰—Žƒ–‘”›’”‘…‡••ǡ–‘‰‡–Š‡”™‹–Š–Š‡‹””‡Žƒ–‹˜‡ƒ…Š‘”‹‰‘ƒ•‡–‘ˆ‹–‡”˜ƒŽ•ǡ™‹ŽŽ ƒŽŽ‘™–Š‡•‹—Ž–ƒ‡‘—•ƒƒŽ›•‹•‘ˆ–Š‡‰‡‡•†‹ˆˆ‡”‡–‹ƒŽŽ›”‡‰—Žƒ–‡†ƒ‘‰–Š‡†‹ˆˆ‡”‡– •ƒ’Ž‡•ƒ†–Š‡‹”’—–ƒ–‹˜‡‰‡‡–‹……‘–”‘Ž‘–Š‡•‡ˆ”—‹–“—ƒŽ‹–›–”ƒ‹–•ǤŠ‹••–—†›…ƒ –Š—• ”‡’”‡•‡– ƒ •–‡’ ˆ‘”™ƒ”†• ‹–‘ –Š‡ …‘’”‡Š‡•‹‘ ‘ˆ –Š‡ •‡˜‡”ƒŽ ‡…Šƒ‹•• —†‡”Ž›‹‰ –Š‡ ƒ’’Ž‡ ˆ”—‹– “—ƒŽ‹–›ǡ ‘ˆˆ‡”‹‰ ‡™ ‘’’‘”–—‹–› ˆ‘” •—’’‘”–‹‰ –Š‡ …‘•–‹–—–‹‘‘ˆ–Š‡‘•–ˆƒ˜‘—”ƒ„Ž‡ƒ’’Ž‡‹†‡‘–›’‡•Ǥ  Identificationofacellnumberregulator(CNR)genefamilyinPrunus  P.DeFranceschi(1),ƒ„”‡”ƒǤ(2)ǡ˜ƒ†‡”ƒƒ’Ǥ(2)ǡ ‡œœ‘‹Ǥ(3)  ȋͳȌ‹˜‡”•‹–›‘ˆ‘Ž‘‰ƒǡ‘Ž‘‰ƒǡ –ƒŽ› ȋʹȌŠ‹‘‰”‹…—Ž–—”ƒŽ‡•‡ƒ”…Šƒ†‡˜‡Ž‘’‡–‡–‡”ǡŠ‡Š‹‘–ƒ–‡‹˜‡”•‹–›ǡ ‘‘•–‡”ǡŠ‹‘ǡ ȋ͵Ȍ‹…Š‹‰ƒ–ƒ–‡‹˜‡”•‹–›ǡƒ•–ƒ•‹‰ǡ‹…Š‹‰ƒǡ  ƒ–—”ƒŽƒ†„”‡‡†‹‰…”‘••’‘’—Žƒ–‹‘•ˆ”—‹–•‹œ‡˜ƒ”‹‡•…‘–‹—‘—•Ž›ǡ„‡Šƒ˜‹‰ƒ•ƒ –›’‹…ƒŽ “—ƒ–‹–ƒ–‹˜‡ –”ƒ‹– ™‹–Š —Ž–‹’Ž‡ Ž‘…‹ ‹…”‡‡–ƒŽŽ› …‘–”‹„—–‹‰ –‘ ƒ† Žƒ”‰‡Ž› ‹ˆŽ—‡…‡†„›‡˜‹”‘‡–ƒŽ…‘†‹–‹‘•Ǥ ‘™‡˜‡”ǡ‹•‘‡…ƒ•‡•ǡƒŠ‹‰Š’”‘’‘”–‹‘‘ˆ –Š‡ ’Š‡‘–›’‹… ˜ƒ”‹ƒ–‹‘ …ƒ „‡ ‡š’Žƒ‹‡† „› ƒ ˆ‡™ “—ƒ–‹–ƒ–‹˜‡ –”ƒ‹– Ž‘…‹ ȋȌǡ …‘””‡•’‘†‹‰–‘‰‡‘‹…”‡‰‹‘•–Šƒ–Ž‹‡Ž›…‘–ƒ‹‘‡‘”‘”‡ƒŒ‘”‰‡‡•™‹–Šƒ •‹‰‹ˆ‹…ƒ–‡ˆˆ‡…–‘ˆ”—‹–•‹œ‡Ǥ‰‡‡‘™–‘”‡‰—Žƒ–‡ˆ”—‹–•‹œ‡ǡ–‘ƒ–‘ˆ™ʹǤʹǡƒ†–™‘ ‘ˆ‹–•ƒ‹œ‡Š‘‘Ž‘‰•ǡƒ‡†ȋ‡ŽŽ—„‡”‡‰—Žƒ–‘”•ȌǡŠƒ˜‡„‡‡•Š‘™–‘‡š‡”– –Š‡‹”‡ˆˆ‡…–‘‘”‰ƒ••‹œ‡„›‘†—Žƒ–‹‰…‡ŽŽ—„‡”Ǥ –Š‡’”‡•‡–•–—†›ǡ–Š‡‰‡‡ ˆƒ‹Ž› ‹ –Š‡ ”‡…‡–Ž› ”‡Ž‡ƒ•‡† ’‡ƒ…Š ȋ”——• ’‡”•‹…ƒȌ ‰‡‘‡ ™ƒ• …Šƒ”ƒ…–‡”‹œ‡†Ǥ  –‘–ƒŽ ‘ˆ ʹ͵  ‰‡‡ •‡“—‡…‡•ǡ •’ƒ‹‰ –Š‡ ‡‹‰Š– ”——• …Š”‘‘•‘‡• ™‡”‡ ‹†‡–‹ˆ‹‡† ƒ† –Š‡‹” •‡“—‡…‡• ™‡”‡ …‘’ƒ”‡† –‘ –Š‡ ͳ͵  ‰‡‡ ˆƒ‹Ž› ‡„‡”• ‹†‡–‹ˆ‹‡†‹ƒ‹œ‡Ǥ‘”‡‘˜‡”ǡ–™‘‘ˆ–Š‡”——••Ž‘…ƒ–‡‹…Ž‘•‡’”‘š‹‹–›‘ˆ–™‘ ‘™…Š‡””›ȋǤƒ˜‹—Ȍˆ”—‹–•‹œ‡•ǡ‘‡‘ˆ™Š‹…ŠŠƒ•„‡‡•Š‘™–‘„‡ƒ••‘…‹ƒ–‡† ™‹–Š†‹ˆˆ‡”‡…‡•‹‡•‘…ƒ”’…‡ŽŽ—„‡”Ǥ˜‡–Š‘—‰Š–Š‡ˆ—…–‹‘ƒŽ…Šƒ”ƒ…–‡”‹œƒ–‹‘ ‘ˆ‰‡‡•‹”——•‹••–‹ŽŽƒ––Š‡„‡‰‹‹‰ǡ–Š‡›’”‘˜‹†‡ƒ•‡–‘ˆ…ƒ†‹†ƒ–‡‰‡‡•‘ˆ ‰”‡ƒ–‹–‡”‡•–ˆ‘”—†‡”•–ƒ†‹‰–Š‡‰‡‡–‹…„ƒ•‡•—†‡”Ž›‹‰‹’‘”–ƒ––”ƒ‹–•”‡Žƒ–‡†–‘ ’Žƒ–ƒ†‘”‰ƒȋ‡Ǥ‰Ǥˆ”—‹–Ȍ•‹œ‡Ǥ  QTLsforbrownspotresistanceinEuropeanpear  P.DeFranceschi(1),ƒ’’ƒ‹ Ǥ(1)ǡ‹”‹ƒ‹Ǥ(2)Ǥǡ‘ŽŽ‹ƒǤ(2)Ǥǡ”—‡ŽŽ‹Ǥ(2)Ǥǡ‘†‹‹Ǥ(1)  ȋͳȌ‡’–‘ˆ ”—‹–”‡‡ƒ†‘‘†›Žƒ–…‹‡…‡•ǡ‹˜‡”•‹–›‘ˆ‘Ž‘‰ƒ‘Ž‘‰ƒǡ –ƒŽ›

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