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New alternative models for in vitro toxicology

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Meeting RepoRts

ALTEX 33(4), 2016

470

research.

Mohammad A. Akbarsha1, Abhijit Majumder2,

Namita Misra3, Christian Pellevoisin4 and Jose Cotovio5

1Mahatma Gandhi-Doerenkamp Center, Bharathidasan

University, Tiruchirappalli, India; 2Department of Chemical

India; 3

Road, Bangalore, India; 4 5

educational purposes.

on cellular and lower animal models, and systems industry, and regulation and implementation.

The outcomes were presented in a common session. This India: Challenges and the road ahead”.

research in India to support networking of the stake-holders, to organize this meeting annually, and to approach the

introduced as a predictive tool for endocrine disruptor effects Dr Susanna Alloisio (ETT, Genoa) presented a sensitive in vitro

(MEA) chips. The major advantages of this in vitro cell-in vitro

to automatically record neuronal activity over several days and followed for days or weeks. The sensitivity, versatility

evaluate more accurately the effects of chemicals on neuronal 2015, 2016).

Dr Marisa Meloni (Vitroscreen, Milan) presented the topic “Adipocytes: scaffold free microtissues for preclinical adipocyte spheroids. These 3D adipose microtissues models for in vitro

in vitro

Research Center, University of Brescia, Italy. The meeting was Brescia. The aim of the meeting was to present new in vitro methodologies and their applications in different areas, from

Francesca Caloni (DIMEVET, University of

in vitro epithelial in vitro 3D epithelial

BME-UV (Al-Bataineh, 2012) and the 3D dog skin equivalent model air-liquid interface (Serra, 2007) to evaluate the

Meeting report

New Alternative Models for

(2)

Meeting RepoRts

ALTEX 33(4), 2016 471

characterization of neuronal network activity for in vitro ,

152-array (MEA) platform as a sensitive tool to detect and evaluate Harmful Algae 55, 230-237.

J. M. et al. (2012). Endocrine disruptors and female Theriogenology 78

theriogenology.2012.06.011

and characterization of a canine skin equivalent. Exp Dermatol 16, 135-142.

porcine jejunal epithelium. PLoS One 8

1, Marisa Meloni2, Yula Sambuy3,

Susanna Alloisio4 and Giovanna Mazzoleni5

1Università degli Studi di Milano, Department of

Veterinary Medicine (DIMEVET), Milan, Italy;

2

Italy; 3

Rome, Italy; 4ETT, Genoa, Italy; 5Università degli Studi

Sciences (DSCS), School of Medicine, Brescia, Italy suspension of cells of one or more cell types to form

scaffold-free spheroids where cells are stimulated to interact with are held in 96-well plates for analysis and characterization

21 days after seeding. In particular, the adipose microtissues

the modulation effects on adipose cell functions derived from treatment with active ingredients, such as food components and additives.

covered distinct aspects of the transition from 2D, single and more physiological tests that are currently emerging in various areas which capture the interaction of different cell

References

Al-Bataineh, M. M., van der Merwe, D., Schultz, B. D. et al.

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