Two integrated platforms to detect source-sink carbon movements in grapevine

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Plants in and for the future Plant micro and macronutrients Plant cell signals X Plant development in a changing

environment Plant facing stress

Two integrated platforms to detect source-sink carbon

movements in grapevine

Davide L. Patono1_{, Daniel Said Pullicino}1_{, Leandro Eloi Alcatrao}1_{, Giorgio Ivaldi}1_{, Andrea}

Firbus1_{, Livio Craveri}1_{, Giorgio Gambino}2_{, Davide Ricauda}1_{, Luisella Celi}1_{, Claudio}

Lovisolo1,2

1_{Dept Agricultural, Forest and Food Sciences, Univ. of Turin, Grugliasco, Italy} 2_{Institute for Sustainable Plant Protection, National Research Council, Turin, Italy}

To study the allocation kinetics of carbon in the different sinks competing in grapevines, a plant

growth chamber for stable isotope labeling has been set in an environmental control system (light intensity, temperature and relative humidity of the air, pot water content) in order to simulate different climate scenarios. Basing on pulse-chasing isotopic strategy, the isotope acted as a tracer of carbon phloem flows.

Furthermore, an open-air plant/soil growth system consisting in twelve independent plant/pot balloons with computing-adjustable air flows allowing continuous gas exchange detection between plants / soil and atmosphere has been set.

The two platforms have been coordinated and used before, during and after induction of drought stress.

Photosynthetic assimilation, stomatal regulation and respiration have been checked in the various phases to size 13_CO

2 enrichment flows in the chamber for stable isotope labeling. Maximum

assimilation ranged from 9 to 12 μmol m-2 _s-1_{, reduced by stomatal control 3 o 4 times at the end of}

the drought period. Saturation of net photosynthesis occurred around 1000 μmol m-2 _s-1 _PPFD:

Key message

Kinetics of carbon are studied toward different sinks competing in grapevines, via pulse-chasing isotopic technique coupled with a system driving adjustable air flows, allowing continuous gas exchange detection between plants / soil and atmosphere.

DIPARTIMENTO TERRITORIO

E SISTEMI AGRO-FORESTALI UNIVERSITA’ DI PADOVA thereafter photoinhibition impairment started in old leaves, whereas young leaves experienced half of maximum assimilation without drastic photoinhibition.

An in-vineyard parallel trial of measurements was used to calibrate and reference pot measurements.