Inducibility, tissue-specificity and product variation of three phytochelatin synthase homeologs from the cadmiumtolerant reed A. donax L.

4

24



688



!

"

#!

$

"%

&'(

)*+

,

-.

,

/0-102

3

45

65

3

73

81091:,

-2

3

;5

3

<

=>?@A

5

3

B3

01C3

.

DDE

3

F

D+

3

GGGGGGGG5

5

5

3

H

0I

BJ-.

+

/K.

3

8DDL3

F

D+

3

.

*13

ME

/0.

3

8,

DE

DNO3

PQ+

DR13

)D0F

1+

10K13

,

03

)DR10*/N102

3

R3

SST

SS5

3

H

48U3

VW@T

@WT

VVX=WYT

?T

W3

3

BE

E

3

Z,

N*.

-3

Z1-1+

:193

[=>?@3

\0,

:1+

-,

.

O3

DF

3

)DR10*/N102

3

]10^/+

L3

_`a

bc

def

gh

a

ei

c

djbf

c

klf

c

mgc

h

gnh

lojpgoq

c

oa

bni

ergoq

c

dloa

s

a

goc

lh

c

oa

bf

h

a

mjf

goc

ta

f

`ljf

c

f

`gc

th

a

f

f

gkc

ngh

da

bba

lkc

ls

c

f

`gc

plnrh

a

u`f

c

`li

ogh

v

c

H

48UI

3

VW@T

@WT

VVX=WYT

?T

W3

MQJE

,

-*193

JO3

]1R/+

.

^10.

3

DF

3

ME

/0.

3

/093

P0:,

+

D0^10.

/E

3

4K,

10K1-2

3

\0,

:1+

-,

.

O3

DF

3

)DR10*/N102

3

8wE

DC-:1x

3

?W2

3

]6T

?@W>3

y+

191+

,

L-J1+

N3

<

*.

.

RI

z

z

RE

105

LQ5

9Lz

10NE

,

-*z

A

5

3

12345678289 81  !"#$% &''()**+++,-./0(123'450106789,0/6*

:;<=:>

;?@=AB

CB

A@>

<;DDB

EEFF>

G

:;<H

>

>

IJKLMN

OPQ

R

ST

KLSKLN

UN

VWON

OPXR

Q

Y

N

Z[IWN

\]^

_`a

b^

c

de

fg

e

hfi`]jkl`]N

m`ic

n

e

fg

e

op

k]c

e

k]qe

r]_^

a

f]s`]c

kp

e

tu^

`]u`be

e

e

vR

Q

T

KN

IJKLSSwLN

N

x`uj]^

ukp

e

\]^

_`a

b^

c

de

fg

e

m`]ska

yN

m`ic

n

e

fg

e

z^

fc

`uj]fp

flde

k]qe

z^

fs`q^

u^

]`N

N

N

IT

K{

XLN

|XLSSwLN

UN

}ZI[vY

N

I[[I

N

\s`~e

\]^

_`a

b^

c

dN

\s`~e

op

k]c

e

tu^

`]u`e

h`]c

a

`N

€Q

KN

VKPXLLN

‚R

Kƒ

SKLN

N

„Xƒ

…wQ

†N

‡LR

JKQ

SR

T

ˆN

‰KŠT

‹

N

w{

N

OPKR

ST

Q

ˆN

XLMN

vR

wSŒR

KLŒKSN

IT

KŠPXLN

KLŽKƒ

N



N

I[[I

N

\]^

_`a

b^

c

de

fg

e

hfi`]jkl`]N

m`ic

n

e

fg

e

op

k]c

e

k]qe

r]_^

a

f]s`]c

kp

e

tu^

`]u`bN

KLQ

R

ŽN

vQ

R

LŒPN

[KMKQ

SKLN

N

‘ka

j’be

\]^

_`a

b^

c

dN

m`ic

n

e

fg

e

“fp

`u’p

ka

e

z^

fp

flde

k]qe

”`]`c

^

ubN

Iwƒ

JKR

†N

Q

w†PN

OPQ

R

ST

R

XLSKLN

N

\]^

_`a

b^

c

de

fg

e

hfi`]jkl`]N

m`ic

n

e

fg

e

op

k]c

e

k]qe

r]_^

a

f]s`]c

kp

e

tu^

`]u`bN

I•Q

KLN

–

—Q

S†XXQ

MN

˜XS™SSKLN

N

\]^

_`a

b^

c

de

fg

e

hfi`]jkl`]N

m`ic

n

e

fg

e

op

k]c

e

k]qe

r]_^

a

f]s`]c

kp

e

tu^

`]u`bN

šR

Q

XN

„Q

ŠKN

vKLMKJR

SN

N

\]^

_`a

b^

c

de

fg

e

hfi`]jkl`]N

m`ic

n

e

fg

e

op

k]c

e

k]qe

r]_^

a

f]s`]c

kp

e

tu^

`]u`be

N

N

>

›<B

FAEB

œB

<>

;?@=AB

CB

A@>

<;DDB

EEFF>

G

›;<H

>

>

OXT

PR

KN

šXQ

T

R

LN



N

IWON

OPXR

Q

N

fj]e

ž

]]`be

h`]c

a

`e

N

‡Q

T

KN

IŒPƒ

ŸT

KQ

N



N

IWON

Ow

ŒPXR

Q

N

\]^

_`a

b^

c

de

fg

e

m bb`p

qfa

g

N

|KQ

KˆN

XQ

…R

LSwL

N

\]^

_`a

b^

c

de

fg

e

¡kl`]^

]l`]N

\]^

¢

ƒ

SKN

_`a

Q

XLLKQ

b^

c

de

fg

N

e

ž

]]b£a

’uyN

}X…R

wN

}wQ

LXQ

X

N

\]^

_`a

b^

c

de

fg

e

“^

p

k]N

xj`e

˜w……R

ks`be

KN

X™†P

¤’c

N

c

f]e

ž

]bc

^

c

’c

`N

„ƒ

XLN

IŒP™ƒ

XL

N

¥kc

’a

kp

e

¦`bf’a

u`be

ž

]bc

^

c

’c

`e

§^

]p

k]qe

¨

©\ªr«

N

XQ

rot­N

R

LN

šKT

¬ƒ

X{

{

N

®wQ

†LˆN

‚¯SPwƒ



N

\s`~e

\]^

_`a

b^

c

dN

|KLSN

‘ka

j’be

IT

w™†XXQ

\]^

_`a

b^

M

N

c

dN

šR

ŒPXKƒ

N

[Xƒ

†Q

KL

N

xj`e

\]^

_`a

b^

c

de

fg

e

hfi`]jkl`]N

¡kl`]^

OPQ

R

ST

R

XLN

]l`]e

‹

v‹

\]^

N

vXŒPK

_`a

b^

c

dN

N

>

>

>

:;<=:<>:?

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

@

A

12345678289 81  !"#$% &''()**+++,-./0(123'450106789,0/6*

:

:

:

:

:

:

:

:

:

:

:

:

:

:

:

:

:

:

:

:

:

:

:

_:

_:

:

:

:

:

012345657589853  455458912234858518 984 7851918  78 4253 87 18 221

 !"#$%#&&#'_{() *+&%,"&"}'_(-".,")"'_(/!0"**"1_{() ","+."&2!"3#44"}5 '_{6!7 .!-*89# .!&"#.(+/:-;<6)6/))=/>;?6@3:A(;&B}

1_{C."DE%F"&2!E,"F& !"!"G%7(G/0-/(;&B} 5_{C."DE%F"&2!"G"F(G;+/(;&B}

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`a(%E7#F&"KEB8#7E##,FI%EF &".,H%#7".E,EIF4E*"H"*O8#EI,E.#7E4#B4#"!"N&"#.L SWPQR_`a,E.EF%E&"FF EIF4E*"H"*BEJ4%EFFE!(.!SWPQR_"FEJ4%EFFE!M# &b&"7EF7#%E&8.SWPQRc.! SWPQRaL/&8%EE,E.EF!"F4BE!*!7" 7I%EF4#.F"DEEJ4%EFF"#.".%##&F(.!*#!E!H#%H .*&"#.G+F(F#.*E #DE%EJ4%EFFE!".BEF&&8EB*#.HE%E.8.*E!&#E%.*E&#*!7" 7F&%EFFLdDE%EJ4%EFF"#.#HSWPQR_`a".STZefWXghfhY ijZkfZVZH %&8E%*#.H"%7E!&8E&B4"*48E.#B&B4EFF#*"&E!&##DE%EJ4%EFF"#.#HH .*&"#.G+,E.EFL-FFIF4E*&% .BFEF!E&E*&E!F&&"F&"*BF",."H"*.&!"HHE%E.*EF".&8E7# .&.!F4E*&%HE& %E#H&8EGFFB.&8EF"NE!(O"&8 /!G+1.!/!G+'4%#! *".,(%EF4E*&"DEB(&8E8",8EF&.!#OEF&7# .&#H&#&GF".BEF&*EFL/!G+' FB.&8EF"NE!&8EF7E7# .&#HG1(G5.!Gl(O8"EM#&8/!G+1.!/!G+5E.NB7EF4%#! *E!F",."H"*.&B 8",8E%7# .&F#HG1.!G5*#74%E!&#GlL

3KE.&#,E&8E%(&8EFE%EF &F".!"*&E&8&&8E,E.E&"*MFEF#HS^YWXVZ[8",8*4M""&B&#&#E%&E&8E4%EFE.*E#H8EDB 7E&F"F(&EF&".4%&(%E&E!&#&8E8",8H .*&"#.F4E*""N&"#.#H"&FPQR,E.EFH%#7&%.F*%"4&"#.FOEF E.NB7&"*4#".&#HD"EOL38 F(&%.F*%"4&"#..E#H .*&"#."N&"#..!F4E*""N&"#.FEE7F&#8DE4BE!7m#%%#E". &8EED# &"#.#H!&#E%.*E".S^YWXVZ[L

n