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Berries aroma phenomics by PTR-ToF-MS

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Acta Italus Hortus 20

Riassunti dei lavori presentati alle

XI Giornate Scientifiche SOI

Bolzano, 14-16 settembre 2016

Organizzate dalla

Libera Università di Bolzano

in collaborazione con

Centro di Sperimentazione Agraria e Forestale Laimburg

Fondazione Edmund Mach di San Michele all’Adige

Volume a cura di

(2)

Qualità delle produzioni

38

MONALISA - A collaborative multi-sensor

approach for non-destructive prediction of

apple fruit quality

Zanella Angelo1, Nadja Sadar1, Giovanni Agati2, Peter

Robatscher1, Wouter Saeys3, Rob Schouten4, Pol

Tijskens4, Lorenzo Spinelli5, Pieter Verboven6, Michael

Oberhuber1

angelo.zanella@provinz.bz.it

1Research Centre for Agriculture and Forestry Laimburg, Ora, Bolzano

2 Istituto di Fisica "Nello Carrara" (IFAC), Consiglio Nazionale delle Ricerche, Sesto Fiorentino

3BIOSYST-MeBioS, Katholieke Universiteit, Leuven, Belgio 4 Horticulture and Product Physiology, Wageningen University, Olanda

5 Istituto di Fotonica e Nanotecnologie (IFN), Consiglio Nazionale delle Ricerche, Milano

6 Department of Agro-Engineerng and Economics, Katholieke Universiteit, Leuven, Belgio

Since the apple fruit industry faces significant post-har-vest losses due to inferior quality of only a small fraction of it, current research efforts aim to develop effective ways for the assessment and the prediction of apple fruit quality and storage potential. In the present collaboration, solu-tions were sought in a multidisciplinary approach. To this end apple cultivars Nicoter/Kanzi(r), Braeburn and Gala from different altitudes, European production regions, and agronomic conditions, were analyzed with various non-destructive optical and acoustic techniques. Fruit were measured with non-destructive sensors based on VIS inte-ractance, chlorophyll fluorescence and acoustic firmness. Using statistical procedures, such as probelation and indexed non-linear- and quantile regression, kinetic models for describing post-harvest colour and texture evolution were calibrated on the basis of the data with promising results. Moreover, spatially- and time-resolved NIR spec-troscopy used for developing empirical models describing and predicting flesh firmness, revealed clear differences between cultivars, and the production regions. For evalua-ting the potential of computer tomography (CT) for non-destructive assessment of internal defects, different settings for 2D and 3D X-ray CT were tested. While the simple radiography did not perform satisfactorily, the CT enabled spatio-temporal monitoring of the development of internal defects and microstructure, and allowed the calculation of algorithms for detecting defects in CT scans of whole fruit. Apples were also measured with NIR spectroscopy and the standard methods of HPLC-DAD and UV/VIS. PLS models were developed for alpha farnesene, conjugated trienols (scald), and antioxidants. The results of this resear-ch could lead to further improvement of pre- and post-har-vest management of apple fruit quality.

Keywords: fruit quality, non-destructive techniques,

spec-troscopy, X-ray, kinetic modelling

Berries aroma phenomics by PTR-ToF-MS

Farneti Brian, Iuliia Khomenko, Alberto Algarra, Marcella Grisenti, Matteo Ajelli, Paula Poncetta, Luca Cappellin, Lara Giongo, Franco Biasioli

brian.farneti@fmach.it

Centro Ricerca e Innovazione, Fondazione Edmund Mach, San Michele all'Adige

Aroma is one of main factors impacting fruit and vege-tables quality and consumer appreciation. Since aroma involves the perception of a pletora of volatile organic compounds (VOCs), their assessment is crucial to guaran-tee the selection and marketability of high quality fruits. High priority should thus be given to replacing poor flavor cultivars with favorable ones, exploiting the variability already available in nature. However, the analysis of the aroma trait in a large number of samples, necessary to over-come the usually massive biological and genetic variability among samples, may be laborious and time consuming. The application of Proton Transfer Reaction -Time of Flight-Mass Spectrometry (PTR-ToF-MS) has been recently described and demonstrates that it is a powerful high-throu-ghput phenotyping tool for both genetic and quality related studies. This contribute describes the application of PTR-ToF-MS for the study of the aroma variability present in the germplasm collection of the main berries species availa-ble at the Foundation E. Mach, with particular regard to strawberry (Fragaria x ananassa), raspberry (Rubus), and blueberry (Vaccinium). The rapidity and the moderate cost of PTR-ToF-MS analysis allowed us to perform a detailed aroma characterization of each species with a peculiar attention to the VOC fold changes caused by ad hoc storage experiments tailored to simulate the “from farm to fork” chain. The results obtained in these investigations gave important explanatory information to be implemented in the breeding programs.

Keywords: VOCs, blueberry, raspberry, strawberry,

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