Functional characterization of fire blight resistance in malus fusca fine mapping, cloning and characterization of fire blight resistance genes from Malus fusca

6thRosaceousGenomicsConference

Mezzocorona,Italy–30th_{SeptemberͲ04}th_{October2012}    ͻͲ  ƒ”‡”Ǧƒ••‹•–‡†•‡Ž‡…–‹‘ȋȌ‹•‘‡‘ˆ–Š‡ƒ‹ƒ’’Ž‹…ƒ–‹‘•‘ˆ‰‡‘‹…”‡•‡ƒ”…Š–‘ „”‡‡†‹‰Ǥ‡†‡•…”‹„‡–Š‡‹‹–‹ƒŽ•–‡’•–ƒ‡„›–Š‡Ǧ ’‡ƒ…Š„”‡‡†‹‰’”‘‰”ƒ –‘™ƒ”†•‹–•”‘—–‹‡•‡Ž‡…–‹‘ˆ‘”ˆ”—‹–•Šƒ’‡ȋ”‘—†ǡˆŽƒ–ƒ†ƒ„‘”–‹‰Ȍƒ†ˆ”—‹–ƒ…‹†‹–› ȋƒ…‹†˜•Ǥ•—„ƒ…‹†ȌǤ –‡‰”ƒ–‹‘ ‘ˆ  ‹–‘ ƒ „”‡‡†‹‰ ’”‘‰”ƒ ”‡“—‹”‡•ǣ ƒȌ –Š‡ ‹†‡–‹ˆ‹…ƒ–‹‘ ‘ˆ ƒŽŽ‡Ž‡• –‹‰Š–Ž› Ž‹‡† –‘ –ƒ”‰‡– –”ƒ‹–•ǡ „Ȍ –Š‡ ‹–‡‰”ƒ–‹‘ ‘ˆ –Š‡ ‰‡‘–›’‹‰ •–‡’ –‘ –Š‡ ™‘” ’‹’‡Ž‹‡‘ˆ–Š‡„”‡‡†‹‰’”‘‰”ƒƒ†…Ȍ–Š‡ƒ†ƒ’–ƒ–‹‘‘ˆŠ‹‰ŠǦ–Š”‘—‰Š’—–’”‘–‘…‘Ž•ˆ‘” ‡š–”ƒ…–‹‘ƒ†‰‡‘–›’‹‰Ǥ ‡†‡…‹†‡†–‘•–ƒ”–™‹–Šˆ”—‹–•Šƒ’‡ƒ†ˆ”—‹–ƒ…‹†‹–›ǡ–™‘•‹’Ž›‹Š‡”‹–‡†–”ƒ‹–• ƒŽ”‡ƒ†›ƒ’’‡†‹–Š‡”——•”‡ˆ‡”‡…‡ƒ’ƒ†–Šƒ–ƒ”‡‡›„”‡‡†‹‰–ƒ”‰‡–•Ǥ‹‡† ƒŽŽ‡Ž‡• –‘ –Š‡•‡ ‰‡‡• ™‡”‡ ˆ‘—† „ƒ•‡† ‘ •‹‰Ž‡Ǧˆƒ‹Ž› ƒƒŽ›•‹• ƒ† Žƒ–‡” ˜ƒŽ‹†ƒ–‡† —•‹‰ ™‹†‡ ‰‡”’Žƒ• …‘ŽŽ‡…–‹‘•Ǥ  ˜‡”› ˆ‡™ …—Ž–‹˜ƒ”• –Š‡ ‹†‡–‹ˆ‹‡† ‘Ž‡…—Žƒ” ƒ”‡”• ™‡”‡ ‘– —•‡ˆ—ŽǤ …‡ –Š‡ ƒ’’”‘’”‹ƒ–‡† ƒŽŽ‡Ž‡• ™‡”‡ ‹†‡–‹ˆ‹‡†ǡ ‘Ž‡…—Žƒ” ƒ”‡”• ™‡”‡ ‰‡‘–›’‡† †—”‹‰ –™‘ ›‡ƒ”• ‹ †‹ˆˆ‡”‡– ’”‘‰‡‹‡• ‘ˆ –Š‡ „”‡‡†‹‰ ’”‘‰”ƒǤ ‡ •–ƒ”–‡† ™‹–Š ’Žƒ–• –Šƒ– ™‡”‡ ƒŽ”‡ƒ†› ‹ –Š‡ ˆ‹‡Ž† ’”‘†—…‹‰ ˆ”—‹–• –‘ ‡•–‹ƒ–‡ •‡Ž‡…–‹‘ ‡ˆˆ‹…‹‡…›Ǥ ˆ–‡” ‰‡‘–›’‹‰ ƒ† ’Š‡‘–›’‹‰ ‘”‡ –Šƒ ͸ͲͲ ‹†‹˜‹†—ƒŽ•ˆ‘”‡ƒ…Š–”ƒ‹–ǡ–Š‡’”‡†‹…–‹‘™ƒ•‘”‡–ŠƒͻͷΨƒ……—”ƒ–‡ǤŠ‡•‡…‘†›‡ƒ” ™ƒ•ƒ’’Ž‹‡†ƒ––Š‡•‡‡†Ž‹‰•–ƒ‰‡™‹–Šͳͻʹ‹†‹˜‹†—ƒŽ•ˆ”‘†‹ˆˆ‡”‡–…”‘••‡•ǤŽŽ ’Žƒ–•™‡”‡–”ƒ•ˆ‡””‡†–‘–Š‡ˆ‹‡Ž†‘”†‡”‡†„›’Š‡‘–›’‡’”‡†‹…–‹‘•ǤŽ•‘‹–Š‹•’Žƒ– …ƒ•‡’”‡†‹…–‹‘™ƒ•…‘””‡…–‘”‡–ŠƒͻͷΨ‘ˆ–Š‡–‹‡•Ǥ‡ƒ”‡…—””‡–Ž›•–ƒ”–‹‰–‘—•‡ ˆ‘”†‹•…ƒ”†‹‰—™ƒ–‡†‰‡‘–›’‡•™‹–Š‘—–ƒ›ˆ—”–Š‡”–‡•–‹‰Ǥ  FunctionalcharacterizationoffireblightresistanceinmalusfuscaǦfinemapping, cloningandcharacterizationofFireBlightresistancegenesfromMalusfusca  O.Emeriewen(1,2,3)ǡ‹‹Ǥ(1)ǡ‹Ž‹ƒǤ(4)ǡƒŽ‘›Ǥ(1)ǡ‡‹ŽǤ(2)  ȋͳȌ_{‡•‡ƒ”…Šƒ†‘˜ƒ–‹‘‡–”‡ǡ ‘†ƒœ‹‘‡†—†ƒ…Šǡ‹ƒǤƒ…ŠǡͳȂ͵ͺͲͳͲ} ƒ‹…Š‡Ž‡ƒŽŽǯ†‹‰‡ȋ”‡–‘Ȍǡ–ƒŽ› ȋʹȌ _{—Ž‹—•òŠǦ•–‹–—–ȋ Ȍǡ ‡†‡”ƒŽ‡•‡ƒ”…Š‡–”‡ˆ‘”—Ž–‹˜ƒ–‡†Žƒ–•ǡ•–‹–—–‡ˆ‘”} ”‡‡†‹‰‡•‡ƒ”…Š‘‘”–‹…—Ž–—”ƒŽ ”—‹–”‘’•ǡ‹ŽŽ‹–œ‡”ǡŽƒ–œ͵ƒǡͲͳ͵ʹ͸‹ŽŽ‹–œǡ ”‡•†‡ǡ‡”ƒ› ȋ͵Ȍ _{‘‘†ƒ†‰”‹…—Ž–—”ƒŽ…‹‡…‡ǡ‡…Š‘Ž‘‰›ƒ†‹‘–‡…Š‘Ž‘‰›…Š‘‘Žǡ‹˜‡”•‹–›‘ˆ} ‘†‡ƒƒ†‡‰‰‹‘‹Ž‹ƒǡ‹ƒ‹˜‡”•‹–ƒǡͶͶͳͳʹͳǡ‘†‡ƒǡ–ƒŽ› ȋͶȌ_{”‹–‹…ƒ”–‡Ȁǡ‘š͹ͳͶͳǡƒ””ƒŽ—Žƒǡƒ„‡””ƒǡʹ͸ͲͲǡ—•–”ƒŽ‹ƒ}  ‹”‡„Ž‹‰Š–ȋ Ȍǡ…ƒ—•‡†„›–Š‡‰”ƒǦ‡‰ƒ–‹˜‡„ƒ…–‡”‹—ǡ”™‹‹ƒƒ›Ž‘˜‘”ƒȋƒȌǡ‹•–Š‡ ‘•–‹’‘”–ƒ–„ƒ…–‡”‹ƒŽ†‹•‡ƒ•‡‘’‘‡ˆ”—‹–™‘”Ž†™‹†‡‹…Ž—†‹‰ƒ’’Ž‡ǤŽ–Š‘—‰Š–Š‡ —•‡ ‘ˆ ƒ–‹„‹‘–‹…• ƒ• ƒ …‘–”‘Ž ‡ƒ•—”‡ Šƒ• „‡‡ •—……‡••ˆ—Ž –‘ •‘‡ †‡‰”‡‡ǡ –Š‡‹” ƒ’’Ž‹…ƒ–‹‘ ‹• —™ƒ–‡†ǡ ˆ‘”„‹††‡ ‘” •–”‹…–Ž› ”‡‰—Žƒ–‡† ‹ ƒ› …‘—–”‹‡• †—‡ –‘ –Š‡ –Š”‡ƒ–•–Š‡›’‘•‡–‘–Š‡‡˜‹”‘‡–Ǥ‡…‡ǡ–Š‡”‡‹•–Š‡‡‡†ˆ‘”ƒƒŽ–‡”ƒ–‹˜‡‡ƒ• ‘ˆ…‘–”‘ŽǤ‡…‡–Ž›ǡ”‡•‡ƒ”…ŠŠƒ••Š‘™–Šƒ–‰‡‡–‹…”‡•‹•–ƒ…‡…ƒ’Žƒ›ƒ˜‹–ƒŽ”‘Ž‡‹ –Š‡ ƒƒ‰‡‡– ‘ˆ ˆ‹”‡ „Ž‹‰Š– †‹•‡ƒ•‡Ǥ  †‹ˆˆ‡”‡– ™‹Ž† •’‡…‹‡• ‘ˆ ƒŽ—•ǡ •‡˜‡”ƒŽ ’‘–‡–‹ƒŽ•‘—”…‡•–‘ ”‡•‹•–ƒ…‡Šƒ˜‡„‡‡‹†‡–‹ˆ‹‡†ƒ†ƒ•ƒ”‡•—Ž–ǡ•‘‡™‹Ž†ƒ’’Ž‡ ƒ……‡••‹‘• Šƒ˜‡ „‡‡ —–‹Ž‹•‡† ƒ• •‘—”…‡• ‘ˆ ”‡•‹•–ƒ…‡Ǥ ‘•‡“—‡–Ž›ǡ †‹ˆˆ‡”‡– “—ƒ–‹–ƒ–‹˜‡–”ƒ‹–Ž‘…‹ȋ•Ȍˆ‘” ”‡•‹•–ƒ…‡Šƒ˜‡„‡‡‹†‡–‹ˆ‹‡†„›‡‹Ž‡–ƒŽǤʹͲͲ͹Ǣ

6thRosaceousGenomicsConference

Mezzocorona,Italy–30th_{SeptemberͲ04}th_{October2012}    ͻͳ  —”‡Ž‡–ƒŽǤʹͲͲͻǡƒ†ƒ””ƒ˜‹…‹‹‡–ƒŽǤʹͲͳͳƒ†ƒŽ•‘„›ƒŽ‡‰‡‡–ƒŽǤʹͲͲͷǢŠƒ‡–ƒŽǤ ʹͲͲ͸Ǣ‹™‹Ž†ƒ’’Ž‡ƒ……‡••‹‘•ƒ†…—Ž–‹˜ƒ–‡†ƒ’’Ž‡ȋƒŽ—•š†‘‡•–‹…ƒȌ”‡•’‡…–‹˜‡Ž›Ǥ Š‡•‡ • ‡šŠ‹„‹– †‹ˆˆ‡”‡– Ž‡˜‡Ž• ‘ˆ ”‡•‹•–ƒ…‡ –‘ –Š‡ †‹•‡ƒ•‡Ǥ ‹–Š –Š‡ ƒ‹ ‘ˆ ‹†‡–‹ˆ›‹‰ǡ‹•‘Žƒ–‹‰ƒ†…Šƒ”ƒ…–‡”‹œ‹‰”‡•‹•–ƒ…‡–‘ˆ‹”‡„Ž‹‰Š–ˆ”‘–Š‡™‹Ž†•’‡…‹‡•Ǥ ˆ—•…ƒǡ™‡”‡’‘”–Š‡”‡–Š‡ˆ‹”•–”‡•—Ž–•‘ˆƒƒŽ›•‹•ƒ†ƒ”‡”†‡˜‡Ž‘’‡–ˆ‘”ˆ‹‡ ƒ’’‹‰Ǥ ͳ ’”‘‰‡‹‡• ‘ˆ ƒŽ—• ˆ—•…ƒ š †ƒ”‡† ™‡”‡ ‰”ƒˆ–‡† ƒ† ‹‘…—Žƒ–‡† ‹ –Š‡ ‰”‡‡Š‘—•‡ ™‹–Š Ǥ ƒ›Ž‘˜‘”ƒ •–”ƒ‹ ƒʹʹʹ ‹ –™‘ …‘•‡…—–‹˜‡ ›‡ƒ”•Ǥ ”ǡ  ƒ† ƒ”‡”•™‡”‡†‡˜‡Ž‘’‡†ƒ†—•‡†–‘‡•–ƒ„Ž‹•Šƒ‰‡‡–‹…ƒ’ƒ†–‘’‡”ˆ‘” ƒƒŽ›•‹•Ǥ  ƒŒ‘”  …‘—Ž† „‡ Ž‘…ƒŽ‹•‡† ‘ ͳͲǤ ™‘ ” ƒ”‡”• ™‡”‡ …Ž‘•‡Ž› Ž‹‡† –‘ –Š‡ Ǥ ‘—”  ƒ”‡”• Ž‹‡† –‘ ”‡•‹•–ƒ…‡ ™‡”‡ †‡˜‡Ž‘’‡† —•‹‰ –Š‡ ”‡ˆ‡”‡…‡ •‡“—‡…‡ ‘ˆ ‘Ž†‡ ‡Ž‹…‹‘—• ˆ‘” ˆ‹‡ ƒ’’‹‰ –Š‡ Ǥ Š‹• ”‡•‡ƒ”…Š ‹• ‹ ’”‘‰”‡••ƒ†ƒ††‹–‹‘ƒŽŽ›ƒ„ƒ…–‡”‹ƒŽƒ”–‹ˆ‹…‹ƒŽ…Š”‘‘•‘‡ȋȌŽ‹„”ƒ”›‘ˆǤˆ—•…ƒ™‹ŽŽ „‡•…”‡‡‡†™‹–Š•ˆŽƒ‹‰–Š‡Ǥ  AnalysisofflavonoidregulationinRosaceae R.V.Espley(1)ǡƒ˜ƒ‰Ž‹ƒǡǤ(2)ǡ‡”›Ǧ‹”ǡǤ(1)ǡ‹Ǧƒ‰ǡǤ(1)ǡƒŽ‡”ǡ Ǥ(1)ǡ‘•–ƒǡǤ(2)ǡ ŽŽƒǡǤǤ(1) (1)Žƒ–ƒ† ‘‘†‡•‡ƒ”…Šǡ—…Žƒ†ǡ‡™‡ƒŽƒ† (2)‡’ƒ”–‡–‘ˆ ”—‹–”‡‡ƒ†‘‘†›Žƒ–…‹‡…‡•ǡ‹˜‡”•‹–›‘ˆ‘Ž‘‰ƒǡ‘Ž‘‰ƒǡ –ƒŽ›  Š‡ˆŽƒ˜‘‘‹†’ƒ–Š™ƒ›’”‘˜‹†‡•’Š‡‘Ž‹……‘’‘—†•–Šƒ–’Žƒ›ƒŒ‘””‘Ž‡•‹‘•ƒ…‡‘—•ˆ”—‹– …‘Ž‘—”ǤŠ‡•‡…‘’‘—†•ƒŽ•‘†‡–‡”‹‡ƒ›‘ˆ–Š‡†‹‡–ƒ”›Š‡ƒŽ–ŠǦ”‡Žƒ–‡†’”‘’‡”–‹‡•‹„‘–Š –Š‡’‡‡Žƒ†…‘”–‡šǤ‡‰—Žƒ–‹‘‹•’”‹ƒ”‹Ž›…‘†—…–‡†„›ƒ…‘’Ž‡š‘ˆ–”ƒ•…”‹’–‹‘ˆƒ…–‘”• ȋ •Ȍǡ…‡–”ƒŽ–‘™Š‹…Šƒ”‡–Š‡ •Ǥ•‹‰‡šƒ’Ž‡•ˆ”‘’‡ƒ…Šƒ†ƒ’’Ž‡ǡ™‡’”‘˜‹†‡ ˆ—…–‹‘ƒŽƒƒŽ›•‹•ˆ‘”–Š‡…‘–”‘Ž‘ˆˆŽƒ˜‘‘Ž•ǡ’”‘ƒ–Š‘…›ƒ‹†‹•ȋ•Ȍƒ†ƒ–Š‘…›ƒ‹•„› –Š‡ˆƒ‹Ž›Ǥ’‡ƒ…Šǡ–Š‡’ƒ––‡”‘ˆ ‰‡‡‡š’”‡••‹‘…‘””‡Žƒ–‡•™‹–Š •™Š‹…Š—’Ǧ ”‡‰—Žƒ–‡ƒ–Š‘…›ƒ‹„‹‘•›–Š‡•‹•ȋͳͲƒ†„͵Ȍǡ‘””‡’”‡••ȋͳͳͳƒ†ͳ͸Ȍ –”ƒ•…”‹’–‹‘‘ˆ„‹‘•›–Š‡–‹…‰‡‡•ǤŠ‡‡š’”‡••‹‘‘ˆƒ’‘–‡–‹ƒŽǦ”‡‰—Žƒ–‹‰–”ƒ•…”‹’–‹‘ ˆƒ…–‘”ǡͳǡ…‘””‡•’‘†•™‹–ŠŽ‡˜‡Ž•Ǥ —…–‹‘ƒŽƒ••ƒ›••Š‘™–Šƒ–ͳͲ’‘•‹–‹˜‡Ž› ”‡‰—Žƒ–‡•–Š‡’”‘‘–‡”•‘ˆ ƒ† „—–‘–Ǥ…‘–”ƒ•–ǡͳ–”ƒ•Ǧƒ…–‹˜ƒ–‡•–Š‡ ’”‘‘–‡”•‘ˆ ƒ†ǡ„—–‘– ǡ•—‰‰‡•–‹‰‡š…Ž—•‹˜‡”‘Ž‡•‘ˆƒ–Š‘…›ƒ‹ƒ† ”‡‰—Žƒ–‹‘Ǥƒ’’Ž‡–Š‡ˆŽƒ˜‘‘‹†„‹‘•›–Š‡–‹…’ƒ–Š™ƒ›‹•‘•–ƒ…–‹˜‡‹–Š‡•‹ǡ™‹–Š–Š‡ ˆŽƒ˜ƒǦ͵Ǧ‘Ž•ǡ…ƒ–‡…Š‹ƒ†‡’‹…ƒ–‡…Š‹ǡƒ…–‹‰ƒ•–Š‡‹‹–‹ƒ–‹‰—‹–•ˆ‘”–Š‡•›–Š‡•‹•‘ˆ ’‘Ž›‡”•Ǥ‡Šƒ˜‡‡šƒ‹‡†–Š‡‰‡‡•‹˜‘Ž˜‡†‹’”‘†—…–‹‘‹†‹ˆˆ‡”‡–ƒ’’Ž‡…—Ž–‹˜ƒ”• ƒ†•Š‘™–Šƒ–ˆŽƒ˜ƒǦ͵Ǧ‘Ž„‹‘•›–Š‡•‹•‹•—†‡”–Š‡…‘–”‘Ž‘ˆ„‹‘•›–Š‡–‹…‡œ›‡•ƒ† ͳǤŠ‡•‡•–‡’•ƒ”‡—†‡”–Š‡…‘–”‘Ž‘ˆ†‡˜‡Ž‘’‡–ƒŽƒ†‡˜‹”‘‡–ƒŽ•–‹—Ž‹ǡ•—…Šƒ• –‡’‡”ƒ–—”‡ƒ†Ž‹‰Š–Ǥ‡ƒ–‹‰ˆ”—‹–”ƒ’‹†Ž›”‡†—…‡•‡š’”‡••‹‘‘ˆ–Š‡–”ƒ•…”‹’–‹‘ƒŽƒ…–‹˜ƒ–‹‘ …‘’Ž‡š”‡•’‘•‹„Ž‡ˆ‘””‡†•‹…‘Ž‘—”ǡ™Š‹Ž‡ƒ•‹‰Ž‡‹‰Š–‘ˆŽ‘™–‡’‡”ƒ–—”‡•‹••—ˆˆ‹…‹‡––‘ ‡Ž‹…‹–ƒŽƒ”‰‡‹…”‡ƒ•‡‹–”ƒ•…”‹’–‹‘‘ˆͳͲƒ†…‘•‡“—‡–Ž›–Š‡„‹‘•›–Š‡–‹…’ƒ–Š™ƒ›Ǥ Analysis of a TFL1 homologous gene and its relationship to the inflorescence variationinJapanesefloweringcherries

T.Esumi(1),Š–ƒ‹ǡǤ(1)ǡ‘•Š‹„ƒ›ƒ•Š‹ǡǤ(1)ǡ‘„ƒ›ƒ•Š‹ǡǤ(1)ǡ•ƒ‘ǡǤ(1)ǡ–ƒ—”ƒǡǤ(1)