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Supplementary Data BUSCA: an integrative web server to predict subcellular localization of proteins

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Supplementary Data

BUSCA: an integrative web server to predict subcellular localization of proteins

Castrense Savojardo, Pier Luigi Martelli, Piero Fariselli, Giuseppe Profiti, Rita Casadio

Supplementary data for Gram+ bacteria

We extracted from UniprotKB/SwissProt all protein sequences belonging to Firmicutes and Actinobacteria phyla and endowed with experimentally annotated subcellular localization. After homology reduction to 25%

sequence identity and filtering-out protein sequences already included into BUSCA training sets, we ended up with 1,667 non-redundant protein sequences whose subcellular localizations are distributed as follows:

510 cytoplasmic, 245 extracellular and 912 plasma membrane.

BUSCA was run in Gram+ mode on this dataset and results are reported in Table S1.

Compartment Precision Recall

MCC

Cytoplasm 0.70 0.96 0.73

Extracellular 0.51 0.45 0.40

Plasma membrane 0.96 0.78 0.74

Supplementary Table S1. Performance evaluation of BUSCA on a Gram-positive dataset derived from UniprotKB.

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