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Characterization of the MLO gene family in Rosaceae and gene expression analysis in Malus domestica

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Academic year: 2021

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CHARACTERIZATION OF THE MLO GENE FAMILY IN ROSACEAE AND GENE EXPRESSION ANALYSIS IN MALUS DOMESTICA

Stefano Pessina1,2, Stefano Pavan3, Domenico Catalano4, Alessandra Gallotta3, Richard G. F. Visser2, Yuling Bai2, Mickael Malnoy1, Henk J. Schouten2

1Fondazione Edmund Mach, Department of Genomics and Biology of Fruit Crops, via E. Mach 1, 38010 San Michele all’Adige, Italy

2Wageningen UR Plant Breeding, Wageningen University and Research Centre, P.O Box 16, 6700 AA Wageningen, The Netherlands

3University of Bari, Department of Soil, Plant and Food Science, Via Amendola 165/A, 70126 Bari, Italy 4National Research Council, Institute of Plant Genetics, Via Amendola 165/A, 70126 Bari, Italy

Contact

stefano.pessina@fmach.it

ABSTRACT Background

Powdery mildew (PM) is a major fungal disease for thousands of plant species, including many cultivated Rosaceae. Specific homologs of the MLO gene family act as PM-susceptibility genes, as their loss-of-function mutations grant durable and broad-spectrum resistance. PM pathogenesis is associated to a pathogen-dependent up-regulation of MLO genes during early stages of infection, causing down-regulation of plant defense pathways.

We carried out a genome-wide characterization of the MLO gene family in apple, peach and strawberry, and we isolated apricot MLO homologs through a PCR-approach. Evolutionary relationships between MLO homologs were studied and syntenic blocks constructed. Homologs that are candidates for being PM susceptibility genes were inferred by phylogenetic relationships with functionally characterized MLO genes and, in apple, by monitoring their expression following inoculation with the PM causal pathogen Podosphaera leucotricha. Genomic tools available for Rosaceae were exploited in order to characterize the MLO gene family. Candidate

MLO susceptibility genes were identified. In follow-up studies it can be investigated whether silencing or a

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