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Rai promotes astrocyte-⁠dependent inflammation during experimental autoimmune encephalomyelitis

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(1)ABSTRACT BOOK. www.eci-vienna2015.org.

(2) Editors: 

(3) ͚͙͘͝”‡•‹†‡– ‹‡–‹Ƥ ”‘‰”ƒ‘‹––‡‡ ‹˜ǤǦ”‘ˆǤ‹’ŽǤǦ

(4) ‰Ǥ”Ǥƒ”„ƒ”ƒ‘ŠŽ‡ Center for Physiology and Pathophysiology Dept. of Pathophysiology/Division of Immunopathology Medical University of Vienna Währinger Gürtel 18-20, 1090 Vienna, Austria [p]: 0043 1 40400 51140 [e]: barbara.bohle@meduniwien.ac.at. 

(5) ͚͙͘͝‘‰”‡••”‡•‹†‡– ƒ‘Ǥ‹˜Ǥ”‘ˆǤ”Ǥ‹ˆ”‹‡† Ǥ‹ Ž Institute of Immunology Medical University of Vienna Lazarettgasse 19, 1090 Vienna, Austria [p]: 0043 1 401 60 33 245 [m]: 0043 664 800 16 65 017 [e]: winfried.pickl@meduniwien.ac.at. ‘‘”†‹ƒ–‹‘ǣ ECI 2015 c/o WMA Ltd. Ž•‡”–”ƒ••‡͜Ȉ͙͘͘͡‹‡ƒȈ—•–”‹ƒ t: +43 1 405 13 83 16 f: +43 1 407 82 74 e: eci2015@medacad.org www.eci-vienna2015.org „•–”ƒ –”‘ ‡••‹‰ǣ Manfred Vodrazka, www.medacad.org ƒ›‘—–ǣƒ”„ƒ”ƒ‹‡‰Žǡ™™™Ǥ„‹‡‰ŽǦ‰”ƒƤǤƒ– Š‘–‘”‡†‹–•ǣUnless otherwise indicated all pictures © ECI – European Congress of Immunology. Copyright © 2015 ECI All rights reserved. No part of this publication may be reproduced, distributed or transmitted without the prior written permission of ECI. Some information may be subject to change..

(6) ‡†‡•†ƒ›ǡ‡’–‡„‡”͡ǡ͚͙͘͝ ‘ Ž—•‹‘•ǣŠ‡•‡Ƥ†‹‰••—‰‰‡•––Šƒ–’”‘Ž‹ˆ‡”ƒ–‹‰ ‡ŽŽ•ƒ”‡‘”‡ƒ„—†ƒ–‹ƒ —–‡†‹•‡ƒ•‡•–Šƒ‹ǤŠ‡Š‹‰Š’‡” ‡–ƒ‰‡‘ˆ͠ή ‡ŽŽ•’”‘Ž‹ˆ‡”ƒ–‹‰‹ƒ –‹˜‡Ž‡•‹‘•‘ˆ suggests a major role of these cells in MS pathogenesis. The high number of CCR5+ cells in MS lesions may have therapeutic implications since CCR5 could be targeted as an anti‹ƪƒƒ–‘”›–”‡ƒ–‡–—•‹‰ƒ”ƒ˜‹”‘ Ǥ ǤǤ͛͜Ǥ͙͡ ƒ‹’”‘‘–‡•ƒ•–”‘ ›–‡Ǧ†‡’‡†‡–‹ƪƒƒ–‹‘†—”‹‰‡š’‡”‹‡–ƒŽƒ—–‘‹—‡‡ ‡’ŠƒŽ‘›‡Ž‹–‹• C. Ulivieri1, M. Savino1, I. Luccarini2ǡǤ ƒ‹‰Ž‹—Ž‘1, A. Aldinucci2, E. Bonechi2, M. Benagiano2, B. Ortensi3ǡ Ǥ‡Ž‹ ‹3, M. D’Elios2, C. Ballerini2, C. T. Baldari1; 1 University of Siena, Siena, Italy, 2‹˜‡”•‹–›‘ˆ Ž‘”‡ ‡ǡ Ž‘”‡ ‡ǡ

(7) –ƒŽ›ǡ3European Institute of Oncology, Milan, Italy. ƒ‹ȀŠ ‡‰ƒ–‹˜‡Ž›”‡‰—Žƒ–‡•Š͙͟ ‡ŽŽ†‹ơ‡”‡–‹ƒ–‹‘ƒ†Ž—’—•ƒ—–‘‹—‹–›Ǥ‡Šƒ˜‡‹˜‡•–‹‰ƒ–‡†–Š‡’ƒ–Š‘‰‡‹ ‘—– ‘‡‘ˆ–Š‡Š͙͟„‹ƒ•ƒ••‘ ‹ƒ–‡†™‹–Šƒ‹†‡Ƥ ‹‡ ›‘ †‡˜‡Ž‘’‡–ǡ—•‹‰–Š‡‘†‡ŽǤ‡š’‡ –‡†Ž›ǡ™ƒ•Ž‡•••‡˜‡”‡‹ƒ‹ǦȀǦ‹ ‡ ‘’ƒ”‡†–‘–Š‡‹”™‹Ž†Ǧ–›’‡ ‘—–‡”’ƒ”–•†‡•’‹–‡ƒ‡Šƒ ‡†‰‡‡”ƒ–‹‘‘ˆ›‡Ž‹Ǧ•’‡ ‹Ƥ  Š͙͟ ‡ŽŽ•–Šƒ–‹ƤŽ–”ƒ–‡†‹–‘–Š‡Ǥ‡˜‡”–Š‡Ž‡••ǡ™Š‡ƒ†‘’–‹˜‡Ž›–”ƒ•ˆ‡””‡†‹–‘‹—‘†‡Ƥ ‹‡–ƒ‹ήȀޏ‹ ‡ǡ–Š‡•‡ ‡ŽŽ•’”‘‘–‡†ƒ‘”‡•‡˜‡”‡†‹•‡ƒ•‡ ‘’ƒ”‡†–‘™‹Ž†Ǧ –›’‡‡ ‡’ŠƒŽ‹–‘‰‡‹ Š͙͟ ‡ŽŽ•ǤŠ‹•’ƒ”ƒ†‘š‹ ƒŽ’Š‡‘–›’‡™ƒ• ƒ—•‡†„›ƒ†‡ ”‡ƒ•‡†‹ƪƒƒ–‘”›”‡•’‘•‡‘ˆƒ•–”‘ ›–‡•ǡ™Š‹ Š™‡”‡ˆ‘—†–‘‡š’”‡••ƒ‹ǡ–‘

(8) Ǧ͙͟Ǥ ‡ ‡ƒ‹ ’Žƒ›•ƒ‘’’‘•‹–‡”‘Ž‡‹Š͙͟ ‡ŽŽ†‹ơ‡”‡–‹ƒ–‹‘ƒ†ƒ•–”‘ ›–‡ƒ –‹˜ƒ–‹‘ǡ™‹–Š–Š‡Žƒ––‡”†‘‹ƒ–‘˜‡”–Š‡ˆ‘”‡”‹Ǥ ǤǤ͛͜Ǥ͚͘ ƒƒǦ ‘Œ—‰ƒ–‡†›‡Ž‹’‡’–‹†‡•‹†— ‡†‡†”‹–‹  ‡ŽŽȋȌǦ†”‹˜‡–‘Ž‡”‘‰‡‹ ”‡•’‘•‡ M. Rodi1, A. de Lastic1ǡǤ”‡ƒ–•ƒ‹•2, I. Panagoulias1, P. Spadidea1, A. Tapinou3, T. Tselios3ǡ

(9) Ǥƒ–•‘—ƒ•3, P. Papathanasopoulos2ǡǤ‘—œƒ‹1; ‹˜‹•‹‘‘ˆ ‡ƒ–‘Ž‘‰›ǡ‡’–Ǥ‘ˆ

(10) –‡”ƒŽ‡†‹ ‹‡ǡ ƒ —Ž–›‘ˆ‡†‹ ‹‡ǡ‹˜‡”•‹–›‘ˆƒ–”ƒ•ǡƒ–”ƒ•ǡ ”‡‡ ‡ǡ2‡’ƒ”–‡–‘ˆ‡—”‘Ž‘‰›ǡƒ–”ƒ•‹˜‡”•‹–› ‘•’‹–ƒŽǡƒ–”ƒ•ǡ ”‡‡ ‡ǡ 3 ‡’ƒ”–‡–‘ˆŠ‡‹•–”›ǡ‹˜‡”•‹–›‘ˆƒ–”ƒ•ǡƒ–”ƒ•ǡ ”‡‡ ‡Ǥ 1. ǤǤ͛͝

(11) —‡‡‰—Žƒ–‹‘ƒ–ƒ””‹‡”‹–‡•Ǧƒ”–͙ ǤǤ͛͝Ǥ͙͘ — –‹‘‘ˆ–Š‡”›ŽŠ›†”‘ ƒ”„‘‡ ‡’–‘”‡’”‡••‘”†—”‹‰’‘Ž›‹ ”‘„‹ƒŽƒ†’ƒ”ƒ•‹–‹ ‹ˆ‡ –‹‘• J. König1, J. Vorac1, D. Degrandi2ǡǤˆ‡ơ‡”2ǡ Ǥ‡‹‰Šƒ”†–1,3ǡ

(12) Ǥ ڔ•–‡”1; 1 

(13) 

(14) –•‹–—–‡ǡ‘ǡ ‡”ƒ›ǡ2

(15) •–‹–—–‡‘ˆ‡†‹ ƒŽ‹ ”‘„‹‘Ž‘‰›ƒ† ‘•’‹–ƒŽ ›‰‹‡‡ǡò••‡Ž†‘”ˆǡ ‡”ƒ›ǡ3

(16)  ‡‹„‹œǦ

(17) •–‹–—–ˆò”™‡Ž–Ǧ‡†‹œ‹‹• Š‡ ‘”• Š—‰ǡò••‡Ž†‘”ˆǡ. ‡”ƒ›Ǥ The Aryl hydrocarbon Receptor (AhR) is a ligand-activated transcription factor, which directs the metabolism of environmental and endogenous low molecular weight chemicals. ‘”‡”‡ ‡–Ž›ǡ–Š‡ŠŠƒ•„‡‡‹†‡–‹Ƥ‡†ƒ•ƒ’‘–‡–‹—‡”‡‰—Žƒ–‘”ǤŠƒ –‹˜‹–›‹•”‡‰—Žƒ–‡†„›ˆ‡‡†„ƒ ‹Š‹„‹–‹‘–Š”‘—‰Š–Š‡Š‡’”‡••‘”ȋŠȌǤ •‹‰ŠǦ ”‡’‘”–‡”‹ ‡™‡ ‘—Ž†•Š‘™ǡ–Šƒ––Š‡Š‹•ƒ‹Ž›‡š’”‡••‡†‹‹—‡ ‡ŽŽ•‘ˆ–Š‡‹–‡•–‹‡ƒ†•‹ǤŠǦ†‡Ƥ ‹‡–‹ ‡ƒ”‡’”‘–‡ –‡†ˆ”‘‹†— ‡† septic shock, suggesting a connection between the AhR/AhRR-system and the defense against pathogens. In this project, we are analysing the function of the AhRR in systemic and intestinal infections using the Colon Ascendens Stent Peritonitis (CASP) model, which leads to polymicrobial sepsis, and an ileitis model based on oral infection with the parasite Toxoplasma gondiiǤ ‹”•–”‡•—Ž–••Š‘™ƒ•Ž‹‰Š–Ž›Š‹‰Š‡”•—”˜‹˜ƒŽ”ƒ–‡‘ˆŠǦ†‡Ƥ ‹‡–‹ ‡ ‘’ƒ”‡†–‘™‹Ž†–›’‡ ‘–”‘Ž•ƒˆ–‡”•—”‰‡”›Ǥ

(18)  ‘–”ƒ•–ǡŠǦ†‡Ƥ ‹‡–‹ ‡ƒ’’‡ƒ” to be more susceptible to parasite induced ileitis compared to wildtype controls. Since the AhR is also known to regulate the Th17 and Treg cell development, the expression of the AhRR in in vitro‰‡‡”ƒ–‡†‡ơ‡ –‘” ‡ŽŽ•—„•‡–•™ƒ•ƒƒŽ›•‡†Ǥ‡ˆ‘—†–Šƒ––Š‡ŠȀ Ǧ”‡’‘”–‡”‹•‡š’”‡••‡†‹–Š‡ƒŒ‘”‹–›‘ˆh17 and Treg cells, to a lesser extent in Th22, but not in Th1 and Th͚ ‡ŽŽ•Ǥ‘•‹‰‹Ƥ ƒ–†‹ơ‡”‡ ‡•„‡–™‡‡ŠǦ†‡Ƥ ‹‡–ƒ†™‹Ž†–›’‡ ‡ŽŽ•™‹–Š”‡‰ƒ”†–‘ ›–‘‹‡‡š’”‡••‹‘™‡”‡†‡–‡ –ƒ„Ž‡Ǥ††‹–‹‘ƒŽŽ›ǡ™‡™‡”‡ƒ„Ž‡–‘•Š‘™ǡ–Šƒ– ŠǦ†‡Ƥ ‹‡–‹ ‡‡•–ƒ„Ž‹•Š‘”ƒŽ‡†‘–‘š‹–‘Ž‡”ƒ ‡Ǥ. ‡†‡•†ƒ›.

(19) –”‘†— –‹‘ǣ‹ ”‡ƒ•‹‰—„‡”‘ˆ•–—†‹‡•ƒ”‡ˆ‘ —•‹‰‘–Š‡ƒ„‹Ž‹–›‘ˆ–‘Ž‡”‘‰‡‹ •ȋ–•Ȍ–‘‹†— ‡ƒ–‹‰‡Ǧ•’‡ ‹Ƥ ‘Ǧ”‡•’‘•‹˜‡‡••‘”–‘Ž‡”ƒ ‡‹ƒ—–‘‹—‡†‹•‡ƒ•‡• such as multiple sclerosis (MS). We explored the potential of tDCs loaded with mannan-conjugated myelin peptides for MS immunotherapy. Materials and Methods: Peripheral blood monocytes and T-cells were isolated from 2 patients with remitting-relapsing MS and 2 age/sex-matched controls. tDCs were generated from monocytes cultured with IL-4/ GM-CSF/vitD3 for 6d. The resulted tDCs were loaded with myelin peptides conjugated with mannan (or peptide alone) and co-cultured with T-cells±IL-2 for 3 rounds of peptide •–‹—Žƒ–‹‘ȋ–‘–ƒŽ‘ˆ͚͝†ȌǤ‡ŽŽ•™‡”‡ƒƒŽ›œ‡†„›ƪ‘™ ›–‘‡–”›–‘†‡–‡”‹‡–Š‡’Š‡‘–›’‡‘ˆ–•ƒ†–Š‡”‡•—Ž–‹‰Ǧ ‡ŽŽ’‘’—Žƒ–‹‘•ǤŠ‡ ›–‘‹‡•

(20) Ǧ͙ȾȀ

(21) Ǧ͞Ȁ

(22) Ǧ͠Ȁ

(23) Ǧ͙͘Ȁ  ǦȽȀ

(24) Ǧ͙͚’͘͟ƒ†–Š‡Ǧ ‡ŽŽ ›–‘‹‡•

(25) Ǧ͚Ȁ

(26) Ǧ͜Ȁ

(27) Ǧ͞Ȁ

(28) Ǧ͙͘Ȁ

(29) Ǧ͙͟Ȁ ǦȽȀ

(30) Ǧɀǡ™‡”‡‡ƒ•—”‡†„›Ǥ‡•—Ž–•ǣ–••Š‘™‡†ƒ•‡‹Ǧƒ–—”‡’Š‡‘–›’‡ƒ†•‡ ”‡–‡†Ž‘™–‘œ‡”‘ Ž‡˜‡Ž•‘ˆ’”‘‹ƪƒƒ–‘”› ›–‘‹‡•Ǥˆ–‡”†͛‘ˆ ‘Ǧ —Ž–—”‡–Š‡Ž›’Š‘ ›–‡•™‡”‡η͘͡ά͛ή͜ήǡƒ†ƒˆ–‡”–Š‡͙•–ƒ–‹‰‡’”‡•‡–ƒ–‹‘ƒŽŽ™‡”‡†‹ơ‡”‡–‹ƒ–‡†‹–‘‡‘”› (CD3+CD4+CD45RO+) cells. At the end of the culture period with the mannan-myelin-peptide-loaded-tDCs, (i) the median % of nTregs (CD4+CD25+FoxP3+) was 10.8 (x3fold higher –Šƒ‹ —Ž–—”‡•™‹–Š–•ή’‡’–‹†‡ƒŽ‘‡Ȍƒ†ȋ‹‹Ȍ–Š‡Ž‡˜‡Ž•‘ˆ

(31) Ǧ͙͘Šƒ†‹ ”‡ƒ•‡†•‹‰‹Ƥ ƒ–Ž›ȋη͞ˆ‘Ž†Ȍ™Š‡”‡ƒ•–Š‡Ž‡˜‡Ž•‘ˆƒŽŽ‘–Š‡” ›–‘‹‡•™‡”‡Ž‘™–‘œ‡”‘ǤŠ‡”‡•—Ž–•™‡”‡ similar between patient and control cultures. Concusions: Our results indicate that mannan-myelin peptide-loaded tDCs can be eventually used for immunotherapy in MS patients. Š‹•™‘”™ƒ••—’’‘”–‡†„›. Dz‘‘’‡”ƒ–‹‘dz‰”ƒ–͘͡Ǧ͚͙Ǧ͘͞͡Ǥ. ǤǤ͛͝Ǥ͚͘ ‡‰—Žƒ–‹‘‘ˆŠ‡š’”‡••‹‘–Š”‘—‰Š‡˜‹”‘‡–ƒŽŠŽ‹‰ƒ†•‹‹–‡•–‹ƒŽ‹—‡ ‡ŽŽ•—„•‡–• O. SchanzǡǤ”ƒ†•–¡––‡”ǡ Ǥ‡‹‰Šƒ”†–ǡ

(32) Ǥ ڔ•–‡”Ǣ 

(33) Ǧ

(34) •–‹–—–‡ǡ‘ǡ ‡”ƒ›Ǥ The aryl hydrocarbon receptor (AhR) is a ligand activated transcription factor, which senses small environmental chemicals. The AhR is expressed in several types of immune cells like dendritic cells (DC), macrophages, T cells and innate lymphoid cells (ILC). AhR activity is regulated through the AhR repressor (AhRR) via a negative feedback loop. ‡‹˜‡•–‹‰ƒ–‡†Š‡š’”‡••‹‘‹–Š‡‰—–‘ˆŠǦ”‡’‘”–‡”‹ ‡‹†‡’‡†‡ ‡‘ˆ–Š‡’”‡•‡ ‡‘ˆŠŽ‹‰ƒ†•‹–Š‡†‹‡–‘”‹ ”‘„‹‘–ƒ—•‹‰‹ ”‘• ‘’›ƒ†ƪ‘™ ›–‘‡–”›Ǥ

(35) ͙͟ǡ

(36) ɀƒ†

(37) Ǧ͚͚’”‘†— –‹‘™ƒ•ƒ••‡••‡†‹–Š‡•–‡ƒ†›•–ƒ–‡‘”ƒˆ–‡”‡Ž‹ ‹–ƒ–‹‘‘ˆ†‡š–”ƒ•‘†‹—•—Žˆƒ–‡ȋȌ ‘Ž‹–‹•Ǥ ‡ˆ‘—†–Š‡Š–‘„‡‡š’”‡••‡†‹͙͙ ήǡƒ ”‘’Šƒ‰‡•ǡ ‡ŽŽ•ǡ‹–”ƒ‡’‹–Š‡Ž‹ƒŽŽ›’Š‘ ›–‡•ȋ

(38) Ȍƒ†ɀ–ή

(39) ‘ˆ–Š‡•ƒŽŽ‹–‡•–‹‡ƒ† ‘Ž‘ǤŠ‡•‡‘„•‡”˜ƒ–‹‘• ‘—Ž† „‡ƒ†‡‹ ‘˜‡–‹‘ƒŽŽ›Š‘—•‡†ƒ•™‡ŽŽƒ•‰‡”ˆ”‡‡‹ ‡Ǥ

(40) ‹ ‡ˆ‡†ƒŠŽ‹‰ƒ††‡Ƥ ‹‡–†‹‡–ǡŠ™ƒ•‘Ž›‡š’”‡••‡†‹Ǥ —”–Š‡”ǡ™‡‘„•‡”˜‡†ƒ‹˜‡”•‡ ‘””‡Žƒ–‹‘‘ˆ

(41) ͚͚ƒ†Š‡š’”‡••‹‘‹

(42) •ǡ†‡•’‹–‡•‹‹Žƒ”ˆ”‡“—‡ ‹‡•‘ˆ

(43) ͚͚’”‘†— ‹‰ ‡ŽŽ•‹ƒ†‘ ‘—–ƒ‹ƒŽ•ǤɀɁή

(44) ™‡”‡•‹‰‹Ƥ ƒ–Ž›”‡†— ‡†‹–Š‡ ‘Ž‘„—–‘–•ƒŽŽ ‹–‡•–‹‡‘ˆŠǦ†‡Ƥ ‹‡–‹ ‡Ǥ —”Ƥ†‹‰•‹†‹ ƒ–‡–Šƒ–Š‡š’”‡••‹‘‹‹—‡ ‡ŽŽ•‘ˆ–Š‡Žƒ‹ƒ’”‘’”‹ƒƒ‹Ž›†‡’‡†•‘†‹‡–ƒ”›ŠŽ‹‰ƒ†•ƒ†‘–•‘— Š‘–Š‡‹ ”‘„‹‘–ƒǤ‡ƒ††‹–‹‘ƒŽŽ›”‡˜‡ƒŽ‡† ƒ ‘‡ –‹‘„‡–™‡‡Šƒ†

(45) ͚͚‡š’”‡••‹‘ƒ††‹ơ‡”‡–‹ƒŽƒŽ–‡”ƒ–‹‘•‹–Š‡ ‘’‘•‹–‹‘‘ˆ‹–‡•–‹ƒŽ‹—‡ ‡ŽŽ•—„•‡–•Ǥ ǤǤ͛͝Ǥ͛͘ Šƒ‰‡•‘ˆ–Š‡–•‹‰ƒŽŽ‹‰‹„”‘ Š‹ƒŽ‡’‹–Š‡Ž‹ƒŽ ‡ŽŽ• ƒƒơ‡ ––Š‡‹—‡”‡•’‘•‡‹ƒ•–Šƒ K. Dietz1, H. Baarsma2ǡǤڐ‹‰•Š‘ơ2ǡǤ Š‹†–Ǧ‡„‡”1, K. Suttner1; Ǧ‡–”‡‘ˆŽŽ‡”‰›ƒ†˜‹”‘‡–ǡ‡ Š‹• Š‡‹˜‡”•‹–¡–ò Š‡ȋȌǡ ‡ŽŠ‘Ž–œ‡–”‡—‹ Šƒ†‡„‡”‘ˆ ‡”ƒ‡–”‡ˆ‘”—‰‡•‡ƒ” ŠȋȌǡò Š‡ǡ ‡”ƒ›ǡ 2 Ǧ‘’”‡Š‡•‹˜‡‡—‘Ž‘‰›‡–”‡ǡ—†™‹‰Ǧƒš‹‹Ž‹ƒ•Ǧ‹˜‡”•‹–¡–ò Š‡ȋȌǡ ‡ŽŠ‘Ž–œ‡–”‡—‹ Šƒ†‡„‡”‘ˆ ‡”ƒ‡–”‡ˆ‘”—‰‡•‡ƒ” ŠȋȌǡò Š‡ǡ. ‡”ƒ›Ǥ 1. Background: The Wnt signalling pathway is mainly linked to lung developmental processes, but there is recent evidence for contribution of the Wnt signalling to asthma. However the impact of IL-4, a key cytokine in asthma, on the Wnt signalling of bronchial epithelial cells is still unknown. Aim: To investigate the Wnt signalling in the cross-talk of Th2 cells (IL-4) with bronchial epithelial cells. ‡–Š‘†•ǣ”‹ƒ”›Š—ƒ„”‘ Š‹ƒŽ‡’‹–Š‡Ž‹ƒŽ ‡ŽŽ•ȋ •Ȍ™‡”‡•–‹—Žƒ–‡†™‹–Š

(46) Ǧ͜—’–‘͜͠ŠǤŠ‡‡š’”‡••‹‘‘ˆ–ƒ† ‰‡‡•™ƒ•ƒƒŽ›•‡†—•‹‰“ƒ† ‘Ƥ”‡†„› ‡•–‡”„Ž‘–Ǥ’‹–Š‡Ž‹ƒŽ ‡ŽŽ•Ž‹‡•Ǧ͚ƒ†͙͞ ͙͜‘Ǧƒ•™‡ŽŽƒ• •™‡”‡•–‹—Žƒ–‡†™‹–Š•‹‰‹Ƥ ƒ–”‡‰—Žƒ–‡†–•ˆ‘”͚͜Šƒ†–Š‡ ›–‘‹‡ƒ† Š‡‘‹‡’”‘ƤŽ‡™ƒ• measured. ‡•—Ž–•ǣ‹‰‹Ƥ ƒ–‰‡‡‡š’”‡••‹‘ Šƒ‰‡•‘ˆ•‡˜‡”ƒŽ–Ž‹‰ƒ†•ƒ† ”‡ ‡’–‘”•™‡”‡‘„•‡”˜‡†Ǥ–͝ƒ™ƒ•͙Ǥ͛ˆ‘Ž†ȋ͡͝ά

(47) ǡ͙Ǥ͚Ǧ͚Ǥ͘Ȍ—’”‡‰—Žƒ–‡†ƒˆ–‡”͞Šǡ™Š‹Ž‡ ͙͘™ƒ•͞Ǧ͟ fold upregulated over 48h of IL-4 treatment. Wnt4 was time-dependent upregulated with a maximal induction of 3 fold (95% CI, 1.4-10.7) compared to control at 48h. Upregulation of –͝ƒ ‘—Ž†„‡ ‘Ƥ”‡†„›‡•–‡”„Ž‘–Ǥ ‹”•–”‡•—Ž–•”‡˜‡ƒŽƒ‹†— –‹‘‘ˆ͠•‡ ”‡–‹‘„›–͜‹Ǧ͚ƒ†͙͞ ͙͜‘Ǧ ‡ŽŽ•ƒ†„›–͝ƒ‹ •Ǥ ‘ Ž—•‹‘ǣŠ‡”‡•—Ž–•‹†‹ ƒ–‡–Šƒ–

(48) Ǧ͜‹ƪ—‡ ‡•–Š‡‡š’”‡••‹‘‘ˆ•‡˜‡”ƒŽ–•‹‰ƒŽŽ‹‰’”‘–‡‹•‹–Š‡„”‘ Š‹ƒŽ‡’‹–Š‡Ž‹—ǤŠ‡•‡’”‘–‡‹••‡‡–‘„‡ƒ„Ž‡–‘ƒ –‘–Š‡‹—‡ ”‡•’‘•‡˜‹ƒ–Š‡„”‘ Š‹ƒŽ‡’‹–Š‡Ž‹—ǡ•—‰‰‡•–‹‰ƒ ‘–”‹„—–‹‘‘ˆ–Š‡–•‹‰ƒŽŽ‹‰–‘–Š‡‹ƪƒƒ–‹‘‹ƒ•–ŠƒǤ. Abstracts of the 4th European Congress of Immunology - ECI 2015 - Vienna, Austria. 485.

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