• Non ci sono risultati.

A biotechnological approach for the development of an enzyme-based platform with applications in the field of personalized medicine


Academic year: 2021

Condividi "A biotechnological approach for the development of an enzyme-based platform with applications in the field of personalized medicine"

Mostra di più ( pagine)

Testo completo



A biotechnological approach for the development of

an enzyme-based platform with applications in the

field of personalized medicine

Sheila Sadeghi, Gianfranco Gilardi

Department of Life Sciences and Systems Biology, University of Torino, Torino, IT

“Personalized medicine” is a new concept in healthcare, one aspect of which defines the specificity and dosage of drugs according to effectiveness and safety for each patient. Dosage strongly depends on the rate of metabolism which is primarily regulated by the activity of cytochrome P450. In addition to the need for a genetic characterization of the patients, there is also the necessity to determine the drug clearance properties of the polymorphic drug

metabolising enzymes.It is well-recognized that patients administered a particular drug will exhibit significant inter-individual variability in their response to treatment. Unfortunately some patients will fail to respond to the therapy entirely, while some others will suffer dose-related side effects,

resulting in significant costs and fatalities. For these reasons, polymorphism in genes encoding the drug metabolizing cytochromes P450 and flavin-containing monooxygenases is a very important factor that can no longer be neglected in the development of new drugs. Progress in the human genome analysis has recently made it possible to identify a patient’s cytochromes P450 make up by genotype analysis using the AmpliChip CYP450 Test available from Roche Diagnostics. However, genotyping needs a parallel enzyme-based platform capable of rapidly measuring a drug’s pharmacokinetics and clearance by the polymorphic drug metabolising enzymes typical of a given genotype, and to this date, such a platform is not available; this is the subject of the present work.  


Documenti correlati

To better compare the proposed structure to the standard one, Figure 8 presents also the gain for the free space, not ground backed, sinuous slot structure.. This better gain

3 shows a comparison of the DIBs that can be measured in HD 37020, HD 37022 and the reference star HD 24398 with HARPS–N, again in radial velocity scale, using the Doppler shift of

Peptidoglycan consists of poly- saccharides and peptides cross-linked in several steps that include a transpeptidase reaction (Fig. This reaction, which links two peptidoglycan

Non-Oriented Parent-Offspring duos in- ferred with less than 600 Mendelian Errors (ME), indicating the preferred name of the two individuals of the pair (Preferred Name Ind1,

Table legend: R = right; L = left; DLPFC = dorsolateral prefrontal cortex; MT = motor threshold; CANTAB = Cambridge Neuropsychological Test Automated Battery; RVP = Rapid

The QCD multijet background in the 72 search regions of the analysis as determined directly from QCD multijet simulation points, with statistical uncertainties and as predicted

Abstract: We present crystallization experiments concerning a broad range of growth conditions of alkali feldspar and sodalite in trachy-phonolite magma composition during their

Keywords: Measurement Error Model, Structural Equation Models, Item Response Theory, Latent Variable Model.. Categorical Data Analysis

For each fund, the vector x is thus formed by measures of both portfolio compo- sition and manager response to external signals, as follows: 10 attributes related to

Se la Corte può giungere a ritenere infondata la questione di legittimità sollevata su questo profilo, è perché essa accorda alla norma impugnata un sufficiente livello di


Chapter 2 introduces the state of the art for sensor interfaces: the generic sensor interface concept (based on sharing the same electronics among similar applications achieving

del 1946.. 5 avranno conseguenze sulla vita futura del minore devono permettergli di esprimere la sua opinione e tenerla in considerazione, non riconoscere al minore

Una volta accettata questa condizione essenziale, lo spaesamento e lo straniamento cedono via via il passo a una nuova complicità con i narratori, che prendono per mano

The Na-O anti-correlation may be used to separate GCs (hosting multiple stellar populations) from open clusters (OCs, where a single stellar population is present), and first and

conte, ma anche da Genova, intenzionata ad affermare contro Pisa la pro- pria influenza a Saint Gilles, una posizione strategica alla foce del Roda- no, come del resto evidenzia

riprodotte integralmente ma non nella sequenza originale. Dunque la copia di età moderna, redatta per motivi di contenzioso, riproduce nell’insieme l’origina­ le andato

LiGenBuilder is the structures generator module of LiGen. LiGenBuilder first places fragments into the active site. Atoms potentially involved in structure generation are

To download and install an application using the designed system, the final users can access the public Download Page, already described in section 4.1.5, opening a link generated

The Simulink model was improved and converted in discrete time in order to exploit it for the design of the PWM used to regulate the voltage supplied by the H bridge component to

studied the effect of two types of platinum adducts—ethylenediamminedichloroplatinum(II) (en-Pt) and diamminecyclohexylamine-platinum (DACH-Pt)—on both a parental and resistant

Other forms of enforcement, on the other hand, lend themselves to almost complete automation, such as in the case of enforcement over (at least certain types of)

This paper describes the CAWICOMS Workbench for the development of configuration services offering personalized user interaction, as well as distributed configuration of products and