ABSTRACT
Keywords: oxidative stress, ROS, antioxidants, d-ROMs test, BAP test, dog.
In the last years research about reactive chemical species is developed quantitatively but also qualitatively. At moderate concentrations, various free radicals-mediated actions protect cells and they involve physiological roles in the function of a number of cellular signalling pathways. They produce beneficial effects in cellular response to noxae, within inflammatory and immunologic processes, for example in defence against infectious agents. When reactive species are in excess of antioxidant defence system, an oxidative stress occurs. This is a deleterious process that can be an important mediator of damage to cell structures, including lipids and membranes, proteins and DNA.
In this report we present data about canine specie and some practical trials are described. In the first trial, the validation of two simple spectrophotometric tests (for both pro-oxidant and antioxidant status evaluation) in canine specie is reported and also the specific referral ranges are calculated. Some trials were carried out to better understand oxidative stress role in some particular situations such as during exercise in hunting dogs. Effects of two dietary antioxidants were investigated by these methods. Then, some preliminary studies were assessed to introduce a clinical use of these paramethers.
