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New SNP markers for raspberry germplasm genotyping

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(1)6thRosaceousGenomicsConference Mezzocorona,Italy–30thSeptemberͲ04thOctober2012  . .  Genefamilies:ClarifyingtheroleofindividualmembersthroughrealtimeqPCR expressionstudies,thecaseofMald1/prǦ10genesinMalusdomestica  PagliaraniG.(1),ƒ”‹•Ǥ(2)ǡƒ”–ƒ”‹‹Ǥ(2)ǡ˜ƒ†‡‡‰Ǥ(1)  (1)Žƒ–”‡‡†‹‰ǡŽƒ–‡•‡ƒ” Š

(2) –‡”ƒ–‹‘ƒŽǡƒ‰‡‹‰‡ǡŠ‡‡–Š‡”Žƒ†• (2)‡’ƒ”–‡–‘ˆ ”—‹–”‡‡ƒ†‘‘†›Žƒ– ‹‡ ‡•ǡ‹˜‡”•‹–›‘ˆ‘Ž‘‰ƒǡ‘Ž‘‰ƒǡ

(3) –ƒŽ› 

(4) ’Žƒ–‰‡‘‡•ƒ›‰‡‡•‡š‹•–‹—Ž–‹’Ž‡ ‘’‹‡•ƒ•‡„‡”•‘ˆ‰‡‡ˆƒ‹Ž‹‡•ǤŠ‡ Š‹‰Š —„‡” ‘ˆ •‹‹Žƒ” ‰‡‡• ƒ† –Š‡‹” ‘ǦŽ‘ ƒŽ‹œƒ–‹‘ ‘ –Š‡ •ƒ‡ ‰‡‘‹  ”‡‰‹‘ Šƒ’‡” –Š‡ ‡Ž— ‹†ƒ–‹‘ ‘ˆ –Š‡ ‰‡‡–‹  „ƒ•‡ ‘ˆ ”‡Žƒ–‡† –”ƒ‹–•Ǥ ‡ †‡‘•–”ƒ–‡Š‘™  „ƒ•‡†‡š’”‡••‹‘•–—†‹‡•ƒ›„‡‘ˆŠ‡Ž’–‘ Žƒ”‹ˆ›–Š‡”‘Ž‡‘ˆ–Š‡‹†‹˜‹†—ƒŽ‰‡‡•ǤŠ‹• “ ƒ’’”‘ƒ Š ‹• ”‘„—•–ǡ Š‡ƒ’ ƒ† ‡ƒ•› –‘ ƒ’’Ž›Ǥ • ƒ•‡ ™‡ —•‡ –Š‡ ƒŽ † ͳȀͳͲ ˆƒ‹Ž›‘ˆƒ’’Ž‡ǡ ‘’‘•‡†„›͵ͳ‰‡‡•ǤŠ‹•ˆƒ‹Ž›‹•‹˜‘Ž˜‡†‹ˆ‘‘†ƒŽŽ‡”‰›ȋ”‡ˆ‡””‡† –‘ ƒ• ƒŽ † ͳȌ ƒ• ™‡ŽŽ ƒ• ‹ –Š‡ †‡ˆ‡ ‡ •›•–‡ ‘ˆ –Š‡ ’Žƒ– ȋ”‡ˆ‡””‡† –‘ ƒ• ǦͳͲȌ ™Š‡”‡„›–Š‡„‹‘Ž‘‰‹ ƒŽˆ— –‹‘‹••–‹ŽŽ— Ž‡ƒ”Ǥ‡†‡˜‡Ž‘’‡†‰‡‡•’‡ ‹ˆ‹ ’”‹‡”’ƒ‹”• ˆ‘”ƒŽŽ–Š‡͵ͳ‰‡‡•Ǥ’‡ ‹ˆ‹ ‹–›™ƒ•˜ƒŽ‹†ƒ–‡†„‘–Š‹•‹Ž‹ ‘ƒ†‹˜‹˜‘Ǥš’”‡••‹‘™ƒ• •–—†‹‡† ˆ‹”•–Ž›ǡ ‘ ›‘—‰ ƒ’’Ž‡ Ž‡ƒ˜‡• ˆ”‘ •—• ‡’–‹„Ž‡ ƒ† ”‡•‹•–ƒ– ‰‡‘–›’‡• ƒˆ–‡” ŠƒŽŽ‡‰‹‰™‹–Š–Š‡ˆ—‰—•‡–—”‹ƒ‹ƒ‡“—ƒŽ‹•ǤŠ‡‘„•‡”˜‡††‹ˆˆ‡”‡ ‡•‹‡š’”‡••‹‘ ’”‘ˆ‹Ž‡• ȋ‹–‡•‹–› Ƭ –‹‡ǦŽ‹ ‡•Ȍ ‹†‹ ƒ–‡ †‹ˆˆ‡”‡–‹ƒ–‹‘ ‹ „‹‘Ž‘‰‹ ƒŽ ˆ— –‹‘•Ǥ ‡ ‘†Ž›ǡ‡š’”‡••‹‘™ƒ••–—†‹‡†‘’‡‡Žƒ†ˆŽ‡•Š‘ˆˆ”—‹–•ˆ”‘ —Ž–‹˜ƒ”•–Šƒ–†‹ˆˆ‡”‹ ƒŽŽ‡”‰‡‹ ‹–›Ǥ Ž› ’ƒ”– ‘ˆ –Š‡ ˆƒ‹Ž› ™ƒ• ‡š’”‡••‡† ‹ ˆ”—‹– ƒ† ƒ Ž‡ƒ” †‹ˆˆ‡”‡–‹ƒŽ ‡š’”‡••‹‘™ƒ•ˆ‘—†„‡–™‡‡–‹••—‡•ƒ•„‡–™‡‡ —Ž–‹˜ƒ”•ǤŠ‹•ƒŽŽ‘™‡†–‘–”‹†‘™ –Š‡—„‡”‘ˆ‰‡‡•–Šƒ– ƒ„‡‹˜‘Ž˜‡†‹ƒ’’Ž‡ƒŽŽ‡”‰›Ǥ‡Š‡”‡„›†‡‘•–”ƒ–‡–Šƒ– “ „ƒ•‡† ‡š’”‡••‹‘ •–—†‹‡• ƒ”‡ —•‡ˆ—Ž –‘ ˆ—”–Š‡” Žƒ”‹ˆ› –Š‡ ”‘Ž‡ ‘ˆ ‹†‹˜‹†—ƒŽ ‡„‡”•‘ˆŽƒ”‰‡‰‡‡ˆƒ‹Ž‹‡•Ǥ  NewSNPmarkersforraspberrygermplasmgenotyping  L.Palmieri(1)ǡ ‹‘‰‘(1)  ȋͳȌ‡•‡ƒ” Š ƒ†

(5) ‘˜ƒ–‹‘ ‡–”‡Ǥ ‘†ƒœ‹‘‡ †—† ƒ Šǡ ‹ƒ Ǥ ƒ Šǡ ͳ Ǧ ͵ͺͲͳͲ ƒ‹ Š‡Ž‡ƒŽŽǯ†‹‰‡ǡ

(6) –ƒŽ›  ‹ ‡ʹͲͲ͵‘”‡–Š‡ʹͷͲ”ƒ•’„‡””›ȋ—„—•‹†ƒ‡—•Ȍƒ ‡••‹‘•™‡”‡ ‘ŽŽ‡ –‡†ƒ–  ‹ ‘”†‡” –‘ ‰‡‡”ƒ–‡ ƒ ‰‡”’Ž•ƒ ‘ŽŽ‡ –‹‘ǡ ™Š‹ Š ‡‡†‡† –‘ „‡ ‰‡‘–›’‡†Ǥ ‘ †‘ •‘ǡ –‡ ‡™  ƒ”‡”• ™‡”‡ †‡˜‡Ž‘’‡† ‘ ͳͲ ”ƒ•’„‡””› ˜ƒ”‹‡–‹‡•ǡ •–ƒ”–‹‰ ˆ”‘  ƒ”‡”•–”ƒ•ˆ‡””‡†ˆ”‘†‹’Ž‘‹†•–”ƒ™„‡””‹‡•ƒ†ƒ’’Ž‡ȋƒ”‰‡–‡–ƒŽǤǡʹͲͲ͹Ǣƒ”‰‡–‡– ƒŽǤǡʹͲͲͻȌǤ•”‡•—Ž–•‘ˆ•‡“—‡ ‹‰ƒƒŽ›•‹•ǡ‡™”ƒ•’„‡””›•‡“—‡ ‡•‘ˆƒ–Š‘ ›ƒ‹†‹ •›–Šƒ•‡ ȋȌǡ Ž‹’‘š›‰‡ƒ•‡ ȋȌǡ ‡–Ǧƒ—”‡‡ ‘š›†ƒ•‡ ȋȌǡ ›ƒ›Ž ƒŽ ‘Š‘Ž †‡›†”‘‰‡ƒ•‡ ȋȌǡ ‡š•’ƒ•‹ ȋȌǡ †‹Š›†”‘ˆŽƒ˜‘‘Ž ͶǦ”‡†— –ƒ•‡ ȋ Ȍǡ ›–‘•‘Ž‹  ƒ• ‘”„ƒ–‡’‡”‘š‹†ƒ•‡ȋȌǡ•’‡”‹‡•›–Šƒ•‡ȋͷȌǡƒŽ’Šƒƒ›Žƒ•‡ȋȌǡ ƒŽ–‘•‡ –”ƒ•’‘”–‡”ȋȌǡ•‘Ž—„Ž‡‹‘”‰ƒ‹ ’›”‘’Š‘•’Šƒ–ƒ•‡ȋ

(7) Ȍǡ’‘Ž›ƒ‹‡‘š‹†ƒ•‡ȋȌǡ ’‡ –ƒ–‡Ž›ƒ•‡ȋȌ‡œ›‡”‡‰‹‘•™‡”‡‘„–ƒ‹‡†ƒ†–Š‡’‘Ž›‘”’Š‹••’”‡•‡ ‡™ƒ• . ͳͳ͵.

(8) 6thRosaceousGenomicsConference Mezzocorona,Italy–30thSeptemberͲ04thOctober2012  . . ƒ••‡••‡†Ǥ ‹ƒŽŽ›ǡ –Š‡ ‰‡”’Žƒ• ‰‡‘–›’‹‰ ™ƒ• †‘‡ ‘ ƒ„‘—– ŠƒŽˆ ‘ˆ –Š‡ ƒ ‡••‹‘• —„‡” —•‹‰ –Š‡ ‡™ ƒ”‡”•Ǥ Š‡ †ƒ–ƒ ‘„–ƒ‹‡† ™‡”‡ ƒƒŽ›•‡† ™‹–Š ‘™‡”ƒ”‡” ˜Ǥ͵Ǥʹͷǡƒ†•‘ˆ–™ƒ”‡•–‘†‡–‡”‹‡–Š‡‰‡‡–‹ †‹˜‡”•‹–›‘ˆ–Š‡™Š‘Ž‡ ‘ŽŽ‡ –‹‘ƒ†–‘‘„–ƒ‹ƒ’”‡Ž‹‹ƒ”› Ž—•–‡”ƒƒŽ›•‹•ǤŠ‡•‡‘Ž‡ —Žƒ”ƒ”‡”•‹‰Š– „‡Š‡Ž’ˆ—Ž‘–‘Ž›ˆ‘”‰‡”’Žƒ• Šƒ”ƒ –‡”‹œƒ–‹‘„—–ƒŽ•‘ǡ•— Šƒ• ƒ†‹†ƒ–‡‰‡‡•ǡˆ‘” ƒ’’‹‰ǡ•›–‡›•–—†‹‡•™‹–Š‹–Š‡‘•ƒ ‡ƒ‡ˆƒ‹Ž›ƒ†ƒ•ƒ•–ƒ”–‹‰’‘‹–ˆ— –‹‘ƒŽ –‘ƒ”‡”Ǧƒ••‹•–‡†•‡Ž‡ –‹‘ˆ‘””ƒ•’„‡””›„”‡‡†‹‰’”‘‰”ƒ•Ǥ  Silencing MloǦlike susceptibility genes to achieve broadǦspectrum resistance to powderymildewinapple  PessinaS.(1,2)ǡƒ‹Ǥ(1)ǡƒŽ‘›Ǥ(2)ǡ Š‘—–‡ Ǥ(1)  (1)ƒ‰‡‹‰‡Žƒ–”‡‡†‹‰ǤǤ‘šͳ͸ǡ͸͹ͲͲǡƒ‰‡‹‰‡ǡŠ‡‡–Š‡”Žƒ†•ǡ (2) ‘†ƒœ‹‘‡ †—† ƒ Šǡ ‡–”‘ ‹ ‡” ƒ ‡

(9) ‘˜ƒœ‹‘‡ǡ ‡’ƒ”–‡– ‘ˆ ‡‘‹ • ƒ†‹‘Ž‘‰›‘ˆ ”—‹–”‘’ǡ

(10) •–‹–—–‘‰”ƒ”‹‘ƒ‹ Š‡Ž‡ƒŽŽǯ†‹‰‡Ȃ

(11) ǡ‹ƒǤƒ Šͳǡ ͵ͺͲͳͲƒ‹ Š‡Ž‡ƒŽŽǯ†‹‰‡ȋȌǡ

(12) –ƒŽ›  ‘™†‡”› ‹Ž†‡™ ‹• ƒ †‹•‡ƒ•‡ ƒ—•‡† „› ƒ„‘—– ͸ͷͲ ‘„Ž‹‰ƒ–‡ „‹‘–”‘’Š‹  ˆ—‰‹ •’‡ ‹‡•ǡ ƒ’ƒ„Ž‡ ‘ˆ ‘Ž‘‹œ‹‰ ƒ”‘—† ͳͲǡͲͲͲ ’Žƒ– •’‡ ‹‡•ǡ ‹ Ž—†‹‰ ƒ› ”‘’•Ǥ  ’ƒ”–‹ —Žƒ” ‹† ‘ˆ ”‡•‹•–ƒ ‡ –‘ –Š‹• ’ƒ–Š‘‰‡ǡ Šƒ”ƒ –‡”‹œ‡† „› †—”ƒ„‹Ž‹–›ǡ „”‘ƒ†Ǧ•’‡ –”— ‡ˆˆ‡ –‹˜‡‡••ƒ†”‡ ‡••‹˜‡‹Š‡”‹–ƒ ‡ǡ™ƒ•‘„–ƒ‹‡†ˆ‘”–Š‡ˆ‹”•––‹‡‹ƒ„ƒ”Ž‡›Ž‘ —–ƒ–Ǥ Ž‘ ‰‡‡• ‡ ‘†‡ ˆ‘” •—• ‡’–‹„‹Ž‹–› ˆƒ –‘”• ƒ† ƒ”‡ –Š‡”‡ˆ‘”‡ ƒŽŽ‡† •—• ‡’–‹„‹Ž‹–›‰‡‡•ȋǦ‰‡‡•ȌǤ‘••Ǧ‘ˆǦˆ— –‹‘—–ƒ–‹‘•‹–Š‡•‡‰‡‡•Ž‡ƒ†–‘Žƒ ‘ˆ •—• ‡’–‹„‹Ž‹–›ǡ™Š‹ Š‡ƒ•”‡•‹•–ƒ ‡ǤŽ‘‰‡‡•Šƒ˜‡„‡‡ˆ‘—†ƒ†•–—†‹‡†‹ƒ› ’Žƒ–•’‡ ‹‡•ǡ‹ Ž—†‹‰•‡˜‡”ƒŽ ”‘’•ǡ™Š‹Ž‡–Š‡”‡ƒ”‡‘•–—†‹‡•‘ˆ”—‹––”‡‡•›‡–Ǥ—” ™‘”ƒ‹•–‘•–—†›Ž‘‰‡‡•‹ƒ’’Ž‡ǡ‹‘”†‡”–‘ƒ Š‹‡˜‡„”‘ƒ†•’‡ –”—”‡•‹•–ƒ ‡Ǥ ”‡˜‹‘—• ™‘”• ‘ †‹ˆˆ‡”‡– •’‡ ‹‡• ȋ–‘ƒ–‘ǡ ƒ”ƒ„‹†‘’•‹•ǡ ‰”ƒ’‡Ȍ •Š‘™ Š‘™ –Š‡ ‡š’”‡••‹‘Ž‡˜‡Ž•‘ˆ•‘‡‘ˆ–Š‡Ž‘‰‡‡•‹ ”‡ƒ•‡†‹”‡•’‘•‡–‘–Š‡‹‘ —Žƒ–‹‘™‹–Š –Š‡ ’ƒ–Š‘‰‡Ǥ › ‡ƒ• ‘ˆ ƒ „‹‘‹ˆ‘”ƒ–‹  ƒ’’”‘ƒ Šǡ ™‡ Ž‘‘‡† ˆ‘” Ž‘ǦŽ‹‡ ‰‡‡• ‹ ƒ’’Ž‡ ‰‡‘‡ ƒ† ™‡ –‡•–‡† –Š‡‹” ‡š’”‡••‹‘ ƒˆ–‡” ‹‘ —Žƒ–‹‘ ™‹–Š ‘†‘•’Šƒ‡”ƒ Ž‡— ‘–”‹ ŠƒǤ Š”‡‡ ƒ’’Ž‡ —Ž–‹˜ƒ”• Šƒ˜‡ „‡‡ –‡•–‡†ǣ ‘Ž†‡ ‡Ž‹ ‹‘—•ǡ ƒŽƒ ƒ† ”ƒ‡„—”Ǥ ”‡Ž‹‹ƒ”› ƒƒŽ›•‹• ‘†— –‡† ‘ ‘Ž†‡ ‡Ž‹ ‹‘—• •Š‘™ –Šƒ– Ͷ Ž‘ ‰‡‡• ƒ”‡ —’Ǧ”‡‰—Žƒ–‡† „‡ ƒ—•‡ ‘ˆ –Š‡ ‹–‡”ƒ –‹‘ ™‹–Š –Š‡ ’ƒ–Š‘‰‡Ǣ ‘‡ ‘ˆ –Š‡•‡ ‰‡‡• ‘Ǧ Ž‘ ƒŽ‹œ‡™‹–ŠƒǤ —”–Š‡”™‡ƒ”‡•‹Ž‡ ‹‰Ž‘ǦŽ‹‡‰‡‡•—•‹‰ƒ‹–‡”ˆ‡”‡ ‡ ƒ’’”‘ƒ Šǡ ‹ ‘”†‡” –‘ †‡’”‹˜‡ –Š‡ ’ƒ–Š‘‰‡ ‘ˆ ‹–• –ƒ”‰‡–  ƒ†ǡ –Š‡”‡ˆ‘”‡ǡ –‘ ‘„–ƒ‹ ”‡•‹•–ƒ ‡Ǥ  Complementarystrategiesforthebreedingofcultivatedstrawberryforhighlevel ofantioxidants  A.Petit(1),ƒŽŽ°•Ǥ(1)ǡ ‘—••‡ƒ—‡ Ǥ(1)ǡŠƒ”–‹‡”Ǥ(1)ǡ‡‘›‡•Ǥ(2)  (1)‹”‡ˆǡ‘—˜‹ŽŽ‡ǡ ”ƒ ‡ (2)

(13) ǡͳ͵͵ʹǡ‹˜‡”•‹–±‘”†‡ƒ—šǡ‹ŽŽ‡ƒ˜‡†̵”‘ǡ ”ƒ ‡  . ͳͳͶ.

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