Questo studio ha permesso di acquisire nuove informazioni riguardo agli effetti che derivano dall’applicazione in post raccolta di elicitori gassosi, in particolare etilene e CO

(1)

4. Conclusioni

Questo studio ha permesso di acquisire nuove informazioni riguardo agli effetti che derivano dall’applicazione in post raccolta di elicitori gassosi, in particolare etilene e CO

2

, su uve da vino delle cv Sangiovese e Trebbiano, entrambe molto diffuse ed utilizzate in Toscana dai produttori vitivinicoli.

Il trattamento con etilene sulle uve Sangiovese, ha mostrato di poter indurre chiari cambiamenti alla composizione sia delle uve che del vino. In particolare durante la fase di post raccolta è risultato efficace nel mantenere il contenuto in antocianine e nell’indurre la sintesi di composti stilbenici.

Anche il vino derivato dalle uve trattate con etilene mostrava un profilo più ricco in composti fenolici rispetto a quello di controllo. Il fatto che il contenuto in antocianine sia risultato maggiore sia nell’uva trattata con etilene che nel vino ottenuto con essa è di fondamentale importanza perché il Sangiovese è noto per alcune difficoltà di colorazione e per perdere il proprio colore molto facilmente durante l'affinamento e l’invecchiamento. Per quanto riguarda alcuni carotenoidi, il loro contenuto decrescente in seguito al trattamento con etilene sta ad indicare che alcuni processi legati alla maturazione dell’acino di uva possono essere accelerati in seguito al trattamento, non solo durante la maturazione in pianta, ma anche nella fase di stoccaggio. Un maggiore decremento in carotenoidi dovrebbe, in teoria, portare alla formazione di norisoprenoidi, molecole che possono conferire un aroma fruttato e floreale al vino e per questo considerati molto importanti. Dalle analisi enzimatiche di quegli enzimi che alterano la struttura e la composizione delle pareti cellulari, è emerso che alcuni enzimi, la cui attività risulta essere alta durante la maturazione dell’acino di uva, venivano maggiormente attivati dal trattamento con etilene. Questa maggiore attività potrebbe avere contribuito all’incremento del coefficiente di estraibilità delle uve, ovvero all’aumento del loro potere nel rilasciare nel mosto durante la macerazione, composti fenolici, aromatici, zuccheri e risorse azotate. Il contenuto di alcune classi aromatiche nel vino ottenuto con le uve trattate risulta effettivamente aumentato rispetto a quello di controllo. Le β-glucosidasi, enzimi che scindono composti fenolici ed aromatici dagli zuccheri a cui sono legati, sono state attivate in seguito al trattamento con etilene e ciò potrebbe spiegare la diminuzione nel contenuto di vari composti aromatici glicosilati nelle uve trattate con etilene.

Le analisi del profilo aromatico del vino ottenuto dalla pigiatura delle uve trattate con etilene hanno

evidenziato che, mentre la maggior parte dei composti glicosilati mostrava lo stesso contenuto nel

vino di controllo e trattato, per quanto riguarda gli aromi liberi, quindi percepibili, vi era un

sostanziale aumento dei fenoli e dei norisoprenoidi. Il maggiore contenuto sia di fenoli che di

(2)

una maggiore sintesi di tali composti in seguito alla stimolazione da parte dell’elicitore gassoso di alcuni pathway metabolici, anche alla maggiore estraibilità come diretta conseguenza dell’attivazione di enzimi coinvolti nel processo di alterazione delle pareti cellulari.

Il trattamento con CO

2

sulle uve Trebbiano invece ha determinato una minore diminuzione del contenuto in carotenoidi, clorofille e composti fenolici durante la disidratazione post raccolta. Tra i composti fenolici, soprattutto le proantocianidine ed i flavan-3-oli sono risultati meno degradati in nelle uve trattate durante la fase di appassimento. Entrambi i pre-trattamenti al 30 ed al 100% CO

2

hanno infatti mostrato lo stesso effetto su tale classe fenolica. Le proantocianidine rappresentano

composti molto importanti, non solo per la sensazione di astringenza che danno al vino, e che

comunque tende a decrementare più aumenta lo loro polimerizzazione, ma anche per il potere

antiossidante ed antifungino che le caratterizza. Molto probabilmente la minore degradazione dei

sopra citati metaboliti secondari può essere messa in relazione alla minore attività degli enzimi

polifenolo ossidasi e perossidasi riscontrata nelle bacche trattate. Dalle analisi del profilo aromatico

del vino dolce ottenuto, i composti non glicosilati percepibili delle famiglie degli esteri, dei

norisoprenoidi (C13) e dei fenoli volatili, erano presenti maggiormente nel vino passito ottenuto

con le uve di controllo rispetto al “trattato”, mentre i composti glicosilati e quindi potenzialmente

aromatici, appartenenti alle famiglie dei norisoprenoidi (C13) e dei terpeni, erano maggiormente

presenti nel vino ottenuto con le bacche trattate con CO

2

. Da questi risultati, si può prevedere che il

vino ottenuto dalle uve trattate con CO

2

, dovrebbe essere meno aromatico rispetto a quello di

controllo, per via di un minore contenuto di aromi percepibili. Quest’ultimo risultato può essere

considerato interessante se si considera che i C13 ed i terpeni sono i composti aromatici più

apprezzati, e nella loro forma glicosilata possono funzionare da “scorta” di metaboliti meno

ossidabili e degradabili in un vino che per essere considerato un Vin Santo ha bisogno di almeno 2

anni di invecchiamento, e considerato che nei normali processi di vinificazione gli enologi

consigliano di aggiungere enzimi (glicosidasi) che liberano i composti aromatici solo alla fine del

processo. Le poligalatturonasi, risultano inibite durante il trattamento con CO

2

al 100% ed anche

per un periodo successivo al trattamento. Possiamo dunque ipotizzare che anche altri enzimi

connessi con le alterazioni della parete cellulare possano essere stati inibiti. Tra gli enzimi coinvolti

in tali processi vi sono anche le glicosidasi, in particolare le ß-glucosidasi, che scindono gli zuccheri

dalle molecole aromatiche. Una loro inibizione potrebbe spiegare il maggiore contenuto in C13 e

terpeni glicosilati nel vino ottenuto con le uve trattate con CO

2

. Il biossido di carbonio applicato a

concentrazioni alte (30 e 100%) su uva in post raccolta anche per brevi periodi porta ad un

rallentamento generale di molte attività metaboliche, tra cui la respirazione cellulare e soprattutto ad

(3)

una minore attività di quegli enzimi che sono responsabili dell’ossidazione di molte sostanze antiossidanti.

Dalle analisi molecolari delle bacche trattate con CO

2

al 30% è emerso che la buccia è il tessuto che più della polpa risente del trattamento, infatti nella buccia 217 geni subivano un cambiamento nell’espressione, mentre solo 75 nella polpa. L’influenza del trattamento è stata soprattutto a carico di metabolismi ormonali (etilene, ABA) e dell’attività di enzimi di parete.

Per quanto riguarda l’indagine della micoflora delle uve Trebbiano, in seguito alle analisi sono stati

identificati Aspergillus, Botrytis cinerea e Penicillium seguendone lo sviluppo sulle uve sia in

seguito a trattamento gassoso che al processo di appassimento della bacca. Il trattamento con CO

2

al

100% è risultato in grado di diminuire la frequenza della presenza di Botrytis cinerea e Penicillum

spp. per la durata della fumigazione, rappresentando così un potenziale strumento utile nel processo

di vinificazione e di disidratazione, se ripetuto nel tempo, per il contenimento di questi importanti

patogeni della vite responsabili della produzione di tossine, tra cui l’ocratosina (OTA) la cui

presenza nel vino è un problema micotossicologico emergente che tiene in allarme tutto il settore

enologico.

(4)

5. Appendice

5.1 Informazioni Supplementari

Tabella S1: Lista di sonde che sono risultate significative dopo analisi “Significant Analysis for Microarrays (analisi SAM)” effettuate comparando buccia di controllo e buccia trattata con CO₂. “Oligo ID” è il codice assegnato ad ogni sonda (Operon). “TC (v5.0 TIGR)” si riferisce alla sequenza Tentative Consensus (TC) o al singolo ES, nel sito web TIGR (http://compbio.dfci.harvard.edu/tgi/), da cui gli oligo sono stati selezionati. “Description” è il nome assegnato ad ogni TC, come descrivono Rotter et al., (BMC Plant Biology 2009, 9:104). “BINCODE” corrisponde al BIN ed al subBIN (http://www.gabipd.org/projects/MapMan/) assegnati ad ogni Vitis vinifera TC presenti nel set di dati AROS1.

“BINNAME” è la descrizione assegnata ad ogni BIN e ad ogni SubBIN. I valori riportano i risultati delle analisi microarray, espressi come log₂, comparando la tesi di controllo con quella trattata con CO₂.

Oligo I TC (v 5.0 TIGR)

Description Value BINCODE BINNAME

Vv_100038 60

TC6567 7

homologue to UP|CB21_GOSHI (P27518) Chlorophyll a-b binding protein 151, chloroplast precursor (LHCII type II CAB-151) (LHCP), partial (81%)

0.97307593 '1.1.1.1' 'PS.lightreaction.phot osystem II.LHC-II'

Vv_100061 58

CB3431 37

similar to

GP|556367|gb|AAA50310.1|| light- harvesting chlorophyll a/b-binding protein {Prunus persica}, partial (59%)

-0.997763 '1.1.1.1' 'PS.lightreaction.phot osystem II.LHC-II'

Vv_100110 48

CB3414 15

null -0.84587243 '1.1.2.2' 'PS.lightreaction.phot

osystem I.PSI polypeptide subunits' Vv_100044

43

TC5291 9

homologue to UP|Q39640_9ROSI (Q39640) Glycolate oxidase , complete

-1.2968107 '1.2.2' 'PS.photorespiration.g lycolate oxydase' Vv_100110

73

TC6704 7

similar to

GB|AAF24126.1|6690399|AF1216 73 soluble starch synthase {Arabidopsis thaliana} (exp=-1;

wgp=0; cg=0), partial (54%)

-0.87501822 '2.1.2.2' 'major CHO

metabolism.synthesis.

starch.starch synthase' Vv_100030

70 TC5928

5 similar to UP|Q9FPJ7_ARATH (Q9FPJ7) At2g27680, partial (46%)

-0.90851045 '3.5' 'minor CHO metabolism.others' Vv_100043

64

TC6062 9

homologue to

UP|Q9XG67_TOBAC (Q9XG67) Glyceraldehyde-3-phosphate dehydrogenase , partial (35%)

1.6013467 '4.9' 'glycolysis.glyceralde hyde 3-phosphate dehydrogenase' Vv_100107

66

TC6930 6

UP|Q9FZ01_VITVI (Q9FZ01) Alcohol dehydrogenase 2 , complete

1.35402935 '5.3' 'fermentation.ADH'

Vv_100029 53

CB9135 76

'MULTIPLE HITS: 2 |

CB913576;TC58450 | CB913576:

AND TC58450: similar to UP|Q84V96_LOTCO (Q84V96) Aldehyde dehydrogenase 1 precursor , partial (58%)'

-1.2777745 '5.10' 'fermentation.aldehyd e dehydrogenase'

(5)

Vv_100050 02

TC6209 4

similar to UP|UCR10_SOLTU (P46270) Ubiquinol-cytochrome c reductase complex 8.0 kDa protein , partial (96%)

-1.2088848 '9.5' 'mitochondrial electron transport / ATP

synthesis.cytochrome c reductase'

Vv_100067 44

DT0207 57

'MULTIPLE HITS: 2 |

DT020757;TC64309 | DT020757:

AND TC64309: weakly similar to UP|Q9SMT3_ARATH (Q9SMT3) Endo-polygalacturonase-like protein (Glycoside hydrolase family 28 protein), partial (27%)'

-0.9252933 '10.6.3' 'cell

wall.degradation.pect ate lyases and polygalacturonases'

Vv_100069 37

CB9182 70

similar to

GP|29539387|dbj|BAC67662.

pectin methylesterase {Pisum sativum}, partial (3%)

-0.9024706 '10.8.99' 'cell

wall.pectin*esterases.

misc' Vv_100069

06

TC6310 4

similar to

RF|NP_178161.1|15220142|NM_1 06694 NHO1; carbohydrate kinase {Arabidopsis thaliana} (exp=-1;

wgp=0; cg=0) , partial (46%)

-1.07751963 '11.5.1' 'lipid

metabolism.glyceral metabolism.glycerol kinase'

Vv_100093 34

TC6230 7

similar to

GB|CAA81286.1|429153|ATCHM UT chorismate mutase precursor {Arabidopsis thaliana} (exp=-1;

-0.92414961 '13.1.6.2.1' 'amino acid

aromatic

aa.phenylalanine and tyrosine.chorismate mutase'

Vv_100048 85

TC6743 3

similar to UP|Q8S4C2_CAMSI (Q8S4C2) Violaxanthin de- epoxidase, partial (75%)

-0.9776871 '16.1.4.21' 'secondary

metabolism.isoprenoi ds.carotenoids.violax anthin de-epoxidase' Vv_100054

11

BQ7988 95

similar to

GP|28466925|gb|AAO44071.1 At5g23230 {Arabidopsis thaliana}, partial (88%)

-0.93287346 '16.2.99' 'secondary

metabolism.phenylpr opanoids'

Vv_100057 12

TC6884 4

similar to

RF|NP_180489.1|15227060|NM_1 28482 oxidoreductase

{Arabidopsis thaliana} (exp=-1;

-1.94412475 '16.4.1' '26.8'

'secondary metabolism.N misc.alkaloid-like' 'misc nitrilases,

*nitrile lyases, berberine bridge enzymes, reticuline oxidase, troponine reductases' Vv_100091

27

TC5708 9

UP|Q5SGD1_VITVI (Q5SGD1) 9- cis-epoxycarotenoid dioxygenase 1, complete

-1.18171285 '17.1.1.1.10' 'hormone

metabolism.abscisic acid.synthesis- degradation.synthesis .9-cis-

epoxycarotenoid dioxygenase' Vv_100072

17

CB9708 24

homologue to

GP|21435938|gb|AAM54033.1 PIN1-like auxin transport protein {Populus tremula x Populus tremuloides}, partial (25%)

-1.4886092 '17.2.2' 'hormone

metabolism.auxin.sig nal transduction'

(6)

Vv_100088 31

CO8195 67

'MULTIPLE HITS: 3 |

CO819567;TC60535;TC55968 | CO819567: AND TC60535:

similar to UP|Q9LSE7_ARATH (Q9LSE7) Emb|CAB45497.1 (AT3g25290/MJL12_25), partial (53%) AND TC55968: similar to UP|Q9LSE7_ARATH (Q9LSE7) Emb|CAB45497.1

(AT3g25290/MJL12_25), partial (43%)'

-0.92950385 '17.2.3' 'hormone

metabolism.auxin.ind uced-regulated- responsive-activated'

Vv_100040 19

TC6924 0

weakly similar to

UP|Q86B83_DROME (Q86B83) CG33099-PA, partial (7%)

-0.9192681 '17.5.1' 'hormone

metabolism.ethylene.

synthesis- degradation' Vv_100043

70

TC6462 3

similar to UP|ACCO_DIOKA (Q8S932) 1-aminocyclopropane-1- carboxylate oxidase (ACC oxidase) (Ethylene-forming enzyme) (EFE) , partial (30%)

0.89000773 '17.5.1' 'hormone

62

TC6144 4

similar to UP|Q84RC3_NICSY (Q84RC3) Gibberellin 2-oxidase 1, partial (33%)

-1.0210367 '17.5.1' 'hormone

80

TC6016 6

similar to UP|O65313_9ROSI (O65313) Cold-regulated LTCOR12, partial (89%)

2.095159 '17.6.3' 'hormone

metabolism.gibbereli n.induced-regulated- responsive-activated' Vv_100055

72

TC6947 8

similar to UP|Q8RV99_ORYSA (Q8RV99) Serine protease

inhibitor-like protein, partial (53%)

-1.9412776 '20.1' '29.5.5'

'stress.biotic' 'protein.degradation.s erine protease' Vv_100016

45

TC6532 2

similar to

RF|NP_187509.1|15231993|NM_1 11731 heat shock protein binding {Arabidopsis thaliana} (exp=-1;

-1.64763515 '20.2.1' 'stress.abiotic.heat'

Vv_100103 56

TC5423 7

similar to UP|P93499_PHAVU (P93499) DnaJ-like protein (Fragment), partial (57%)

-0.87260455 '20.2.1' 'stress.abiotic.heat'

Vv_100031 98

TC6035 8

similar to UP|Q8LG52_ARATH (Q8LG52) Ankyrin-like protein, partial (24%)

-0.97433065 '20.2.3' 'stress.abiotic.drought /salt'

Vv_100005 55

TC6208 7

weakly similar to

UP|Q8H2A6_ANACO (Q8H2A6) Germin-like protein, partial (87%)

-1.35151975 '20.2.99' 'stress.abiotic.unspeci fied'

Vv_100038 79

TC5691 0

weakly similar to

UP|Q6PV94_SOYBN (Q6PV94) Thioredoxin, partial (80%)

-1.2235358 '21.1' 'redox.thioredoxin'

Vv_100001 20

TC6146 6

weakly similar to

UP|Q41413_SOLTU (Q41413) Epoxide hydrolase, partial (88%)

-0.88168174 '26.1' 'misc.misc2'

Vv_100080 52

CF07454 4

null -0.83041627 '26.13' 'misc.acid and other

phosphatases' Vv_100033

77

TC6305 9

similar to UP|Q589Y2_TOBAC (Q589Y2) Glycosyltransferase NTGT5a, partial (33%)

-0.95701235 '26.2' 'misc.UDP glucosyl and glucoronyl transferases'

(7)

Vv_100059 11

CB0068 95

similar to

GP|3176669|gb|AAC18793.1| End is cut off. {Arabidopsis thaliana}, partial (22%)

-1.1306145 '26.2' 'misc.UDP glucosyl and glucoronyl transferases' Vv_100070

26

CB9207 76

similar to

GP|11072027|gb|AAG28906.1 F12A21.14 {Arabidopsis thaliana}, partial (9%)

-0.86485505 '26.22' 'misc.short chain dehydrogenase/reduct ase (SDR)'

Vv_100101 96

TC6517 8

similar to UP|Q9ZR45_TOBAC (Q9ZR45) Alpha-N-

acetylglucosaminidase, partial (21%)

-0.9667529 '26.8' 'misc.nitrilases,

*nitrile lyases, berberine bridge enzymes, reticuline oxidases, troponine reductases'

Vv_100098 50

TC6739 5

similar to UP|Q9FQE5_SOYBN (Q9FQE5) Glutathione S-

transferase GST 13 , partial (61%)

-1.33110115 '26.9' 'misc.glutathione S transferases'

Vv_100078 55

TC6093 5

similar to UP|Q9FYW5_LYCES (Q9FYW5) BAC19.11, partial (26%)

-1.2775343 '27.1.1' 'RNA.processing.spli cing'

Vv_100051 10

BM4373 59

null -1.49049825 '27.3.11' 'RNA.regulation of

transcription.C2H2 zinc finger family'

Vv_100061 07

CB3422 71

'MULTIPLE HITS: 4 |

CB342271;TC70740;CB980495;T C56316 | CB342271: AND TC70740: similar to

UP|Q9LDL7_ARATH (Q9LDL7) SCARECROW gene regulator-like (Phytochrome A signal

transduction 1 protein), partial (18%) AND CB980495: AND TC56316: similar to

UP|Q9XE53_ARATH (Q9XE53) Scarecrow-like 5 (Fragment), partial (69%)'

-0.99958687 '27.3.21' 'RNA.regulation of transcription.GRAS transcription factor family'

Vv_100138 48

TC6688 1

similar to

GB|AAY28970.1|63054405|DQ00 6269 GIA/RGA-like gibberellin response modulator {Gossypium hirsutum} (exp=-1; wgp=0; cg=0), partial (55%)

-1.24220475 '27.3.21' 'RNA.regulation of transcription.GRAS transcription factor family'

Vv_100041 03

TC5476 2

similar to UP|Q84UB0_MALXI (Q84UB0) Transcription factor Myb1, partial (37%)

-0.89405502 '27.3.26' 'RNA.regulation of transcription.MYB- related transcription factor family' Vv_100042

05

TC6032 2

similar to UP|Q52QR2_SOYBN (Q52QR2) NAC domain protein NAC4, partial (66%)

-0.83619484 '27.3.27' 'RNA.regulation of transcription.NAC domain transcription factor family' Vv_100064

24

CB3478 25

similar to

GP|9955562|emb|CAC05446.1 NAM-like protein {Arabidopsis thaliana}, partial (51%)

-1.05306675 '27.3.27' 'RNA.regulation of transcription.NAC domain transcription factor family'

(8)

Vv_100014 09

TC6141 0

weakly similar to

UP|Q9LV59_ARATH (Q9LV59) Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone:

MOB24, partial (35%)

-0.92600422 '27.3.30' 'RNA.regulation of transcription.Trihelix, Triple-Helix

transcription factor family'

Vv_100055 78

CA8106 54

null -1.0457918 '27.3.8' 'RNA.regulation of

transcription.C2C2(Z n) DOF zinc finger family'

Vv_100088 46

TC6207 3

similar to UP|GAT11_ARATH (O82632) GATA transcription factor 11 (AtGATA-11), partial (28%)

-0.81566639 '27.3.9' 'RNA.regulation of transcription.C2C2(Z n) GATA

transcription factor family'

Vv_100012 53

TC5740 2

similar to

GB|AAF68120.1|7715602|AC0107 93 F20B17.14 {Arabidopsis thaliana} (exp=0; wgp=1; cg=0), partial (6%)

-0.96310577 '27.3.99' 'RNA.regulation of transcription.unclassi fied'

Vv_100032 35

TC5571 9

similar to

RF|NP_173922.1|15222611|NM_1 02362 aspartic-type endopeptidase/

pepsin A {Arabidopsis thaliana}

(exp=-1; wgp=0; cg=0), partial (42%)

Vv_100090 55

TC5607 5

similar to UP|ZF2N2_ARATH (Q9SJM6) Zinc finger A20 and AN1 domains-containing protein At2g36320, partial (75%)

Vv_100143 89

TC6509 3

similar to UP|Q801T3_XENLA (Q801T3) LOC397994 protein (Fragment), partial (3%)

Vv_100098 77

TC6890 7

similar to UP|Q5EN04_MAGGR (Q5EN04) 40S ribosomal protein S18-like protein, complete

-1.0881834 '29.2.1.2.1.18' 'protein.synthesis.ribo somal

protein.eukaryotic.40 S subunit.S18' Vv_100000

21

TC5318 1

homologue to

UP|Q8LJW0_SOYBN (Q8LJW0) 40S ribosomal S4 protein, partial (95%)

0.80421326 '29.2.1.2.1.4' 'protein.synthesis.ribo somal

protein.eukaryotic.40 S subunit.S4' Vv_100073

16

CB9732 78

similar to PIR|D85343|D85343 ribosomal protein S15a homolog [imported] - Arabidopsis thaliana, partial (68%)

-0.97459435 '29.2.1.2.1.515 '

'protein.synthesis.ribo somal

protein.eukaryotic.40 S subunit.S15A' Vv_100079

40

TC5912 5

similar to UP|Q5CZ54_SOLTU (Q5CZ54) Pom14 protein, complete

-1.15740185 '29.3.2' 'protein.targeting.mit ochondria'

(9)

Vv_100005 30

CF20782 6

'MULTIPLE HITS: 4 |

CF207826;TC56666;TC56494;TC 55642 | CF207826: AND TC56666: similar to

UP|Q40264_MESCR (Q40264) Protein kinase, partial (16%) AND TC56494: similar to

UP|SAPK1_ORYSA (Q75LR7) Serine/threonine-protein kinase SAPK1 (Osmotic stress/abscisic acid-activated protein kinase 1) , partial (91%) AND TC55642:

similar to UP|Q40264_MESCR (Q40264) Protein kinase, partial (49%)'

-1.00277225 '29.4' 'protein.postranslatio nal modification'

Vv_100091 77

TC5558 6

similar to UP|Q75WU3_POPNI (Q75WU3) Leucine-rich repeat receptor-like protein kinase 1, partial (12%)

-1.39559475 '29.4.1.57' '30.2.99'

'protein.postranslatio nal

modification.kinase.r eceptor like

cytoplasmatic kinase VII' 'signalling receptor kinases misc' Vv_100101

06

TC5939 4

weakly similar to

UP|Q2L3V1_WHEAT (Q2L3V1) ETEA-like (Expressed in T-cells and eosinophils in atopic dermatitis) protein, partial (42%)

-1.1059017 '29.5' 'protein.degradation'

Vv_100141 45

TC5939 4

weakly similar to

UP|Q2L3V1_WHEAT (Q2L3V1) ETEA-like (Expressed in T-cells and eosinophils in atopic dermatitis) protein, partial (42%)

-1.13567785 '29.5' 'protein.degradation'

Vv_100061 84

CB3433 34

similar to

GP|18844990|dbj|BAB85469. Rad6 {Oryza sativa (japonica cultivar- group)}, partial (98%)

-1.1491691 '29.5.11.3' 'protein.degradation.u biquitin.E2'

Vv_100013 70

TC5538 9

similar to UP|Q2HV66_MEDTR (Q2HV66) Zinc finger, RING- type; RINGv, partial (39%)

-0.85012912 '29.5.11.4.2' 'protein.degradation.u biquitin.E3.RING' Vv_100019

04

CF21266 5

'MULTIPLE HITS: 2 |

CF212665;TC53052 | CF212665:

AND TC53052: similar to RF|NP_199241.1|15241453|NM_1 23795 protein binding

wgp=0; cg=0), partial (4%)'

-1.2833801 '29.5.11.4.2' 'protein.degradation.u biquitin.E3.RING'

Vv_100067 72

CB9146 06

similar to PIR|T51245|T51245 COP1-interacting protein CIP8 [imported] - Arabidopsis thaliana, partial (13%)

Vv_100073 50

TC6582 5

weakly similar to

UP|Q7F1U7_ORYSA (Q7F1U7) Arm repeat containing protein homolog-like, partial (25%)

Vv_100068 73

CB9167 13

null -0.8277472 '29.5.11.4.3.2' 'protein.degradation.u biquitin.E3.SCF.FBO X'

(10)

Vv_100132 55

TC5701 8

null -1.0397035 '29.5.11.4.3.2' 'protein.degradation.u biquitin.E3.SCF.FBO X'

Vv_100141 38

TC5242 5

similar to UP|Q6K649_ORYSA (Q6K649) Kelch repeat-containing F-box protein-like, partial (42%)

-1.2466447 '29.5.11.4.3.2' 'protein.degradation.u biquitin.E3.SCF.FBO X'

Vv_100066 29

TC6331 3

similar to UP|Q52QX8_MANES (Q52QX8) Cysteine protease CP1, partial (40%)

-2.10144435 '29.5.3' 'protein.degradation.c ysteine protease' Vv_100053

85

BQ7985 94

similar to

GP|28207816|emb|CAD55558.

NFU1 protein {Arabidopsis thaliana}, partial (38%)

-0.97866332 '29.8' 'protein assembly and cofactor ligation'

Vv_100032 76

TC5508 7

similar to UP|Q850I2_VITVI (Q850I2) Gag-pol polyprotein (Fragment), partial (23%)

-1.8456953 '30.2.17' 'signalling.receptor kinases.DUF 26' Vv_100051

33

BM4378 68

weakly similar to

PIR|T05181|T05181 S-receptor kinase (EC 2.7.1.-) T6K22.120 precursor - Arabidopsis thaliana, partial (14%)

-1.44540535 '30.2.17' 'signalling.receptor kinases.DUF 26'

Vv_100081 40

CF20853 4

homologue to

GP|6850916|emb|CAB71126.1 calmodulin-binding protein {Cicer arietinum}, partial (18%)

-1.0515781 '30.3' 'signalling.calcium'

Vv_100098 97

TC6485 0

similar to UP|ITPK2_ARATH (Q9SUG3) Inositol-

tetrakisphosphate 1-kinase 2 (Inositol-triphosphate 5/6-kinase 2) (Inositol 1,3,4-trisphosphate 5/6- kinase 2) (AtItpk-2) , partial (75%)

1.22826055 '30.4.5' 'signalling.phosphino sitides.inositol-1,3,4- trisphosphate 5/6- kinase'

Vv_100039 87

TC6519 6

similar to

RF|NP_175635.1|15218189|NM_1 04104 microtubule motor

-1.20940815 '31.1' 'cell.organisation'

Vv_100052 10

BQ7935 15

similar to

SP|P29620|KC47_ORYSA CDC2ﰠ싛related protein kinase R2 (EC 2.7.1.-). [Rice] {Oryza sativa}, partial (24%)

-1.09574805 '31.2' 'cell.division'

Vv_100082 42

TC6948 2

similar to UP|Q8S3W5_HORVD (Q8S3W5) Mei2-like protein, partial (17%)

-0.8988859 '31.2' 'cell.division'

Vv_100106 97

DT0096 13

'MULTIPLE HITS: 2 |

DT009613;TC52209 | DT009613:

AND TC52209: weakly similar to UP|Q84TL6_MAIZE (Q84TL6) Legumin-like protein, partial (39%)'

-0.95067658 '33.1' 'development.storage proteins'

Vv_100108 57

TC5396 7

weakly similar to

UP|Q84TL6_MAIZE (Q84TL6) Legumin-like protein, partial (34%)

(11)

Vv_100036 81

CB9161 44

'MULTIPLE HITS: 2 |

CB916144;TC70852 | CB916144:

AND TC70852: similar to UP|Q9XEE3_DATGL (Q9XEE3) Root-nodule protein Dg93, partial (94%)'

-1.370471 '33.99' 'development.unspeci fied'

Vv_100056 95

CA8151 86

null -1.0358615 '33.99' 'development.unspeci

fied' Vv_100068

78

CB9169 51

similar to PIR|F84542|F84542 nodulin-like protein [imported] - Arabidopsis thaliana, partial (31%)

-1.2405734 '33.99' 'development.unspeci fied'

Vv_100084 24

CF21544 9

similar to

GP|15982206|emb|CAC83608.

Naﱿ antiporter isoform 2 {Lycopersicon esculentum}, partial (32%)

-0.89846267 '34.14' 'transport.unspecified cations'

Vv_100059 24

CB0073 50

null 0.92784865 '34.15' 'transport.potassium'

Vv_100032 02

TC5909 2

similar to

GB|AAC49791.1|2316016|ATU92 650 MRP-like ABC transporter {Arabidopsis thaliana} (exp=-1;

1.3523234 '34.16' 'transport.ABC transporters and multidrug resistance systems'

Vv_100068 34

CB9160 93

similar to

GP|10172595|dbj|BAB01399.

multidrug resistance-associated protein (MRP); ABC-transoprter {Arabidopsis thaliana}, partial (5%)

0.98945377 '34.16' 'transport.ABC transporters and multidrug resistance systems'

Vv_100000 50

BQ7956 58

'MULTIPLE HITS: 3 |

BQ795658;TC68767;TC60678 | BQ795658: AND TC68767:

homologue to

UP|O04892_TOBAC (O04892) Cytochrome P450 like_TBP , partial (21%) AND TC60678:

similar to UP|Q84XC6_9ROSI (Q84XC6) Plasma intrinsic protein 2,2, complete'

-1.0199507 '34.19.1' 'transport.Major Intrinsic Proteins.PIP'

Vv_100037 76

TC6919 0

UP|Q5PXH0_VITVI (Q5PXH0) Aquaporin, complete

-1.01731925 '34.19.1' 'transport.Major Intrinsic Proteins.PIP' Vv_100083

85

CF21399 5

similar to PIR|S52421|S52421 amino acid transport protein AAP2 - Arabidopsis thaliana, partial (37%)

-0.895999 '34.3' 'transport.amino acids'

Vv_100044 28

TC5474 5

weakly similar to

RF|NP_171669.1|15223439|NM_1 00045 transporter {Arabidopsis thaliana} (exp=-1; wgp=0; cg=0), partial (90%)

-0.9684197 '34.99' 'transport.misc'

Vv_100032 22

TC5345 5

similar to

RF|NP_565817.2|42569649|NM_1 29126 transferase, transferring glycosyl groups {Arabidopsis thaliana} (exp=-1; wgp=0; cg=0), partial (11%)

-1.08677767 '35.1' 'not assigned.no ontology'

(12)

Vv_100050 43

TC6386 3

similar to UP|ATX3H_ARATH (Q9M391) Ataxin-3 homolog (Machado-Joseph disease-like protein) (MJD1a-like) , partial (54%)

Vv_100052 62

BQ7956 71

null -0.92972253 '35.1' 'not assigned.no

ontology' Vv_100052

86

BQ7959 03

ontology' Vv_100055

84

TC6874 6

similar to

RF|NP_190638.1|15229830|NM_1 14929 protein binding

Vv_100059 17

CB0070 80

similar to

SP|Q9LDH0|XYLT_ARATH Beta- (1 2)-xylosyltransferase (EC 2.4.2.38). [Mouse-ear cress]

{Arabidopsis thaliana}, partial (13%)

Vv_100067 29

CB9135 74

ontology' Vv_100086

70

TC6613 1

similar to UP|Q93WB2_ARATH (Q93WB2)

AT5g25280/F18G18_20, partial (56%)

Vv_100089 25

TC5174 4

similar to

GB|BAA97483.1|8885553|AB0256 04 ripening-related protein-like;

hydrolase-like {Arabidopsis thaliana} (exp=0; wgp=1; cg=0), partial (79%)

Vv_100101 44

TC5240 5

similar to UP|Q52JK6_NICBE (Q52JK6) VIP2, partial (43%)

1.0217809 '35.1' 'not assigned.no ontology' Vv_100106

41

TC6527 7

similar to UP|Q8LPF0_ARATH (Q8LPF0) At1g73960/F2P9_17, partial (3%)

-0.87504245 '35.1' 'not assigned.no ontology' Vv_100116

74

CA8155 75

'MULTIPLE HITS: 2 |

CA815575;TC62134 | CA815575:

AND TC62134: similar to UP|Q6EPY8_ORYSA (Q6EPY8) SOUL heme-binding protein-like, partial (57%)'

Vv_100124 63

TC6981 5

ontology' Vv_100134

14

TC6746 2

weakly similar to

RF|NP_197744.1|15237783|NM_1 22260 catalytic {Arabidopsis thaliana} (exp=-1; wgp=0; cg=0), partial (64%)

Vv_100142 91

TC5756 1

similar to UP|Q6T284_9ROSI (Q6T284) Predicted protein, partial (22%)

-1.5493165 '35.1' 'not assigned.no ontology' Vv_100066

98

TC6869 9

weakly similar to

UP|Q8W0F9_ORYSA (Q8W0F9) C2 domain-containing protein-like, partial (20%)

-1.02036115 '35.1.19' 'not assigned.no ontology.C2 domain- containing protein'

(13)

Vv_100067 82

CB9148 35

similar to

GP|8778876|gb|AAF79875.1|

T7N9.15 {Arabidopsis thaliana}, partial (31%)

-1.7482722 '35.1.40' 'not assigned.no ontology.glycine rich proteins'

Vv_100083 29

CF21226 4

similar to

GP|15289939|dbj|BAB63634.

P0483G10.28 {Oryza sativa (japonica cultivar-group)}, partial (8%)

-0.88362695 '35.1.41' 'not assigned.no ontology.hydroxyprol ine rich proteins'

Vv_100122 89

TC6734 3

weakly similar to

UP|Q6K9W7_ORYSA (Q6K9W7) Pentatricopeptide (PPR) repeat- containing protein-like, partial (4%)

-1.1646608 '35.1.5' 'not assigned.no ontology.pentatricope ptide (PPR) repeat- containing protein' Vv_100000

07

TC5911 4

'MULTIPLE HITS: 4 |

TC59114;TC66656;CA809148;CA 818007 | TC59114: AND

TC66656: AND CA809148: AND CA818007:'

-1.3476806 '35.2' 'not

assigned.unknown'

Vv_100006 10

CF21026 9

null -1.02804425 '35.2' 'not

assigned.unknown' Vv_100011

23

TC5205 2

similar to UP|Q5QM96_ORYSA (Q5QM96) Rubisco subunit binding-protein beta subunit-like, partial (28%)

-0.81003493 '35.2' 'not

assigned.unknown'

Vv_100011 36

TC6208 3

similar to UP|Q9SJ31_ARATH (Q9SJ31) Expressed protein, partial (60%)

-1.5872741 '35.2' 'not

07

TC5397 7

similar to UP|PAB2_SCHPO (O14327) Polyadenylate-binding protein 2 (Poly(A)-binding protein 2) (Poly(A)-binding protein II) (PABII), partial (10%)

-0.97771435 '35.2' 'not

assigned.unknown'

Vv_100018 06

TC5526 7

similar to UP|Q6NLF5_ARATH (Q6NLF5) At5g18850, partial (55%)

-0.9135298 '35.2' 'not

78

TC6873 2

similar to

GB|AAA64745.1|758367|HSU064 54 AMP-activated protein kinase {Homo sapiens} (exp=-1; wgp=0;

cg=0), partial (3%)

-1.07880325 '35.2' 'not

assigned.unknown'

Vv_100023 80

TC6289 5

similar to UP|Q9LW16_ARATH (Q9LW16) Gb|AAD55593.1 (AT3g15630/MSJ11_3), partial (34%)

-1.13195835 '35.2' 'not

assigned.unknown'

Vv_100025 40

TC5558 1

null -1.1723804 '35.2' 'not

65

TC5813 8

homologue to UP|Q9SEL2_VITVI (Q9SEL2) Gag-pol polyprotein, partial (32%)

-0.89261738 '35.2' 'not

04

TC7112 9

UP|Q56UX3_ONCMY (Q56UX3) HoxA3a-2 (Fragment), partial (6%)

-1.33664625 '35.2' 'not

80

TC7093 3

weakly similar to

UP|Q8VXU9_ARATH (Q8VXU9) AT3g13440/MRP15_7, partial (60%)

-1.072121 '35.2' 'not

assigned.unknown'

(14)

Vv_100028 23

TC6745 5

similar to UP|Q699N2_SCHGA (Q699N2) NADH dehydrogenase subunit 5, partial (4%)

-0.8128275 '35.2' 'not

09

TC5656 3

similar to UP|Q9LKB4_ARATH (Q9LKB4) Arabidopsis thaliana genomic DNA, chromosome 3, TAC clone:K15M2, partial (11%)

-0.94930144 '35.2' 'not

assigned.unknown'

Vv_100035 46

TC6152 7

null -1.4224059 '35.2' 'not

77

TC6362 6

weakly similar to

UP|Q3EBL4_ARATH (Q3EBL4) Protein At2g37035, partial (12%)

-1.2086615 '35.2' 'not

06

TC6463 9

null -0.81036005 '35.2' 'not

05

TC6901 8

null -1.09138205 '35.2' 'not

63

TC6255 1

homologue to UP|Q2I313_9ROSI (Q2I313) Cyclase, partial (8%)

-0.84848855 '35.2' 'not

67

BQ8003 20

'MULTIPLE HITS: 3 |

BQ800320;TC56318;TC61883 | BQ800320: AND TC56318:

weakly similar to

UP|Q7X9S5_GOSBA (Q7X9S5) Fiber protein Fb15, partial (98%) AND TC61883: weakly similar to UP|Q7X9S5_GOSBA (Q7X9S5) Fiber protein Fb15, partial (98%)'

-0.81019024 '35.2' 'not

assigned.unknown'

Vv_100044 90

TC5425 2

weakly similar to

UP|Q2AA50_ASPOF (Q2AA50) Retrotransposon gag protein, partial (11%)

-0.83274174 '35.2' 'not

assigned.unknown'

Vv_100045 16

TC6448 4

homologue to

UP|Q6NN03_ARATH (Q6NN03) At5g04080, partial (54%)

-0.80303733 '35.2' 'not

58

TC5484 8

weakly similar to

UP|DAD2_HORVU (Q9SME8) Defender against cell death 2 (DAD-2), partial (17%)

-0.91161375 '35.2' 'not

assigned.unknown'

Vv_100052 08

TC6769 8

similar to UP|U315_ARATH (Q8LFJ5) UPF0315 protein At1g22270, partial (90%)

-0.85205755 '35.2' 'not

69

BQ7976 72

null -1.0455954 '35.2' 'not

65

CA8102 85

similar to PIR|T09745|T09745 myb-related protein - upland cotton, partial (3%)

-0.89767237 '35.2' 'not

70

CA8104 03

null -0.86281395 '35.2' 'not

09

TC6988 1

similar to UP|AROA_CHLMU (Q9PK28) 3-phosphoshikimate 1- carboxyvinyltransferase (5- enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS) , partial (5%)

-1.47788765 '35.2' 'not

assigned.unknown'

Vv_100057 20

CA8163 64

null -1.75397 '35.2' 'not

assigned.unknown'

(15)

Vv_100057 73

CA8182 93

weakly similar to

GP|21112461|gb|AAM40696.1 outer membrane efflux protein {Xanthomonas campestris pv.

campestris str. ATCC 33913}, partial (4%)

-1.2201148 '35.2' 'not

assigned.unknown'

Vv_100060 11

CB3395 39

weakly similar to

PIR|T09964|T09964 extensin CYC15 precursor - Madagascar periwinkle, partial (17%)

-2.0755497 '35.2' 'not

assigned.unknown'

Vv_100061 99

CB3435 46

similar to

GP|21104591|dbj|BAB93184.

P0031D02.12 {Oryza sativa (japonica cultivar-group)}, partial (16%)

-1.62147055 '35.2' 'not

assigned.unknown'

Vv_100062 26

CB3438 79

similar to

SP|Q9ZM13|DAPA_HELPJ Dihydrodipicolinate synthase (EC 4.2.1.52) (DHDPS).

[Campylobacter pylori J99], partial (5%)

-1.1673493 '35.2' 'not

assigned.unknown'

Vv_100062 66

CB3449 81

similar to

SP|O97159|CHDM_DROME Chromodomain helicase-DNA- binding protein Mi-2 homolog (dMi-2). [Fruit fly], partial (6%)

-1.09503215 '35.2' 'not

assigned.unknown'

Vv_100063 11

CB3460 33

null -0.84143525 '35.2' 'not

57

TC5291 1

weakly similar to

GB|AAL09731.1|15982767|AY057 490 At2g32240/F22D22.1

-0.9482165 '35.2' 'not

assigned.unknown'

Vv_100064 11

CB3476 43

PIR|S54102|S54102 isopenicillin N epimerase (EC 5.1.1.-) -

Lysobacter lactamgenus (strain YK90), partial (2%)

-1.0750978 '35.2' 'not

assigned.unknown'

Vv_100064 28

TC6671 6

UP|Q84NG9_VITVI (Q84NG9) 2S albumin, partial (58%)

-1.1481537 '35.2' 'not

81

CB8379 03

similar to

GP|7300427|gb|AAF55584.1|

CG7709-PA {Drosophila melanogaster}, partial (2%)

-2.083717 '35.2' 'not

assigned.unknown'

Vv_100068 41

TC5889 5

weakly similar to

UP|Q84VZ6_ARATH (Q84VZ6) At2g31140, partial (84%)

-1.1928252 '35.2' 'not

39

TC5952 8

similar to UP|Q9SJV8_ARATH (Q9SJV8) Expressed protein, partial (59%)

-0.9734281 '35.2' 'not

31

CB9232 61

homologue to

GP|4903139|dbj|BAA77836.1 extensive homology to FT (FLOWERING LOCUS T AB027504) and TSF (TWIN SISTER OF FT AB027506) genes, partial (7%)

-1.02443768 '35.2' 'not

assigned.unknown'

(16)

Vv_100074 76

CB9804 94

weakly similar to

GP|15450367|gb|AAK96477.1 AT5g37380/MNJ8_170 {Arabidopsis thaliana}, partial (5%)

-1.1138317 '35.2' 'not

assigned.unknown'

Vv_100076 26

CD0096 63

similar to

GP|1185397|gb|AAA87791.1| SH3 domain binding protein {Rattus norvegicus}, partial (4%)

-0.8920278 '35.2' 'not

assigned.unknown'

Vv_100076 56

CD0120 40

homologue to

GP|12028|emb|CAA33936.1||

ORF82 {Oryza sativa (japonica cultivar-group)}, partial (36%)

-1.20284435 '35.2' 'not

assigned.unknown'

Vv_100082 26

CF21002 9

weakly similar to

GP|1834392|emb|CAA70821.1 Tig {Bacillus subtilis}, partial (10%)

-2.2151439 '35.2' 'not

79

NP5964 94

GB|AF369818.1|AAM21276.1 resistance gene analog [Vitis vinifera]

-1.68603575 '35.2' 'not

79

DV9391 94

'MULTIPLE HITS: 2 |

DV939194;TC59522 | DV939194:

AND TC59522: weakly similar to UP|Q53M57_ORYSA (Q53M57) Expressed protein, partial (45%)'

-1.2626618 '35.2' 'not

assigned.unknown'

Vv_100092 23

TC6380 7

similar to UP|Q2QN68_ORYSA (Q2QN68) Expressed protein, partial (9%)

-1.350937 '35.2' 'not

46

TC7057 4

(Q3FJ89) Single-strand binding protein, partial (17%)

-1.2452357 '35.2' 'not

06

TC6088 7

null -0.80204493 '35.2' 'not

04

TC6191 4

null -0.9100622 '35.2' 'not

20

TC7079 5

null -0.83604196 '35.2' 'not

35

TC6717 5

similar to

GB|AAM19988.1|20466133|AY09 8978 AT3g61870/F21F14_40 {Arabidopsis thaliana} (exp=-1;

-1.3098636 '35.2' 'not

assigned.unknown'

Vv_100098 48

TC6726 6

null -0.92972613 '35.2' 'not

54

TC6899 8

similar to UP|Q4WCV2_ASPFU (Q4WCV2) Actin associated protein, partial (3%)

-1.20264155 '35.2' 'not

75

TC5681 4

weakly similar to

UP|O22102_VICFA (O22102) Retrotransposon-like gene (Fragment), partial (26%)

-1.1623642 '35.2' 'not

assigned.unknown'

Vv_100104 88

TC6640 7

null -1.15651905 '35.2' 'not

91

TC6593 4

null -0.81104588 '35.2' 'not

69

TC6197 8

null -1.06881325 '35.2' 'not

assigned.unknown'

(17)

Vv_100109 07

BQ7951 85

'MULTIPLE HITS: 2 |

BQ795185;TC66566 | BQ795185:

AND TC66566: weakly similar to UP|Q8LRL4_PETHY (Q8LRL4) Nam-like protein 11, partial (16%)'

-0.86752823 '35.2' 'not

assigned.unknown'

Vv_100109 85

TC7112 3

homologue to

GB|CAA67774.1|1480375|AECVP {Escherichia coli} (exp=-1;

-0.9115689 '35.2' 'not

assigned.unknown'

Vv_100118 87

TC6701 0

weakly similar to

UP|Q21II6_9ALTE (Q21II6) Nitrate transport ATP-binding subunits C and D, partial (8%)

-1.13174445 '35.2' 'not

assigned.unknown'

Vv_100120 23

TC6774 8

null -1.27681775 '35.2' 'not

11

TC5650 2

weakly similar to

UP|Q5GAL2_9VIRU (Q5GAL2) Myristylated membrane protein, partial (8%)

-0.94906623 '35.2' 'not

assigned.unknown'

Vv_100121 29

TC5558 1

null -1.1891553 '35.2' 'not

92

TC7085 7

null -0.93630684 '35.2' 'not

19

TC7073 8

null -0.85176543 '35.2' 'not

23

TC6745 5

similar to UP|Q699N2_SCHGA (Q699N2) NADH dehydrogenase subunit 5, partial (4%)

-0.81217835 '35.2' 'not

67

TC6945 5

null -1.11979325 '35.2' 'not

36

TC7017 5

null -0.87342934 '35.2' 'not

75

TC6986 4

null -0.97309562 '35.2' 'not

76

TC6623 6

null -1.04110495 '35.2' 'not

54

TC5243 0

null -1.8017731 '35.2' 'not

91

TC5220 2

null -0.9675721 '35.2' 'not

36

TC6591 5

null -1.49499925 '35.2' 'not

96

TC5726 0

null -0.89451153 '35.2' 'not

13

TC6901 8

null -0.8592746 '35.2' 'not

15

TC6002 7

null -1.00158865 '35.2' 'not

30

TC6200 3

null 1.10014935 '35.2' 'not

70

TC6448 4

homologue to

UP|Q6NN03_ARATH (Q6NN03) At5g04080, partial (54%)

-1.26622155 '35.2' 'not

87

TC5256 6

null -1.18609815 '35.2' 'not

assigned.unknown'

(18)

Vv_100131 60

TC5484 8

weakly similar to

UP|DAD2_HORVU (Q9SME8) Defender against cell death 2 (DAD-2), partial (17%)

-1.2070422 '35.2' 'not

assigned.unknown'

Vv_100132 80

TC5564 3

similar to UP|Q1ZFZ9_9GAMM (Q1ZFZ9) Galactose-1-phosphate uridylyltransferase, partial (5%)

-0.8428892 '35.2' 'not

assigned.unknown'

Vv_100132 93

TC5574 4

'MULTIPLE HITS: 2 |

TC55744;TC65345 | TC55744:

AND TC65345: weakly similar to UP|Q2HUL7_MEDTR (Q2HUL7) Integrase, catalytic region; Zinc finger, CCHC-type, partial (4%)'

-2.251302 '35.2' 'not

assigned.unknown'

Vv_100133 25

TC5787 9

similar to UP|Q28ID8_XENTR (Q28ID8) OTTXETP00000012322 (Fragment), partial (16%)

-1.0521225 '35.2' 'not

assigned.unknown'

Vv_100136 86

TC5592 7

weakly similar to

UP|Q96316_ARATH (Q96316) Blue-copper binging protein III (Uclacyanin 3), partial (9%)

-2.02591595 '35.2' 'not

assigned.unknown'

Vv_100137 14

TC5533 2

similar to UP|Q24WY9_DESHA (Q24WY9) GTP cyclohydrolase II, partial (5%)

-0.97518684 '35.2' 'not

98

TC6251 2

null -0.87156328 '35.2' 'not

06

TC6566 5

null -0.87939277 '35.2' 'not

15

TC6764 8

similar to

GB|AAH78174.1|50418095|BC078 174 MGC4268 protein {Homo sapiens} (exp=-1; wgp=0; cg=0), partial (7%)

-1.20661 '35.2' 'not

assigned.unknown'

Vv_100140 52

TC7020 2

null -0.86659962 '35.2' 'not

76

TC7037 3

similar to UP|Q22AP7_TETTH (Q22AP7) Protein kinase domain, partial (4%)

-1.21051895 '35.2' 'not

61

TC6515 3

null -0.85385225 '35.2' 'not

28

TC6603 0

null -0.92237158 '35.2' 'not

58

TC6844 9

homologue to

PRF|2118402L|1582523|2118402L YBR1013 gene. {Saccharomyces cerevisiae} (exp=-1; wgp=-1; cg=- 1), partial (8%)

-1.3347302 '35.2' 'not

assigned.unknown'

Vv_100000 02

TC6499 5

similar to UP|Q9XGN4_AJURE (Q9XGN4) Galactinol synthase, isoform GolS-1 , partial (46%)

-1.1723512 '35.3' 'disagreeing hits'

Vv_100016 33

TC6999 1

weakly similar to

RF|NP_172089.1|15221358|NM_1 00479 calcium ion binding {Arabidopsis thaliana} (exp=-1;

(19)

Vv_100036 64

TC6573 2

similar to UP|Q2XPV1_SOLTU (Q2XPV1) Drm3-like protein-like protein, partial (41%)

Vv_100040 99

TC5899 6

similar to UP|Q9LE80_ARATH (Q9LE80) Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone: MJK13

(AT3g15450/MJK13_11)

(MJK13.11 protein), partial (94%)

0.81972428 '35.3' 'disagreeing hits'

Vv_100043 65

CB9145 57

'MULTIPLE HITS: 2 |

CB914557;TC56030 | CB914557:

AND TC56030: homologue to UP|Q7FAH2_ORYSA (Q7FAH2) OJ000223_09.15 protein, partial (98%)'

Vv_100061 48

CB3430 42

similar to

GP|1785675|emb|CAA69780.1 orf107a {Arabidopsis thaliana}, partial (52%)

Vv_100062 79

TC6774 0

ribosomal protein L20 [Vitis vinifera]

-1.364584 '35.3' 'disagreeing hits' Vv_100065

57

CB3501 75

similar to

GP|1794252|gb|AAB41308.1|

albumin seed storage protein precursor {Juglans regia}, partial (17%)

Vv_100067 04

DT0066 62

'MULTIPLE HITS: 2 |

DT006662;TC56474 | DT006662:

AND TC56474: similar to

UP|Q9MA11_ARATH (Q9MA11) F20B17.7, partial (28%)'

Vv_100073 57

CB9753 38

null -0.97926905 '35.3' 'disagreeing hits'

Vv_100136 53

TC5372 8

similar to UP|O48628_PRUAR (O48628) Pyrophosphate-

dependent phosphofructo-1-kinase (Fragment), partial (88%)

Vv_100008 99

TC6943 7

similar to UP|RL26A_ARATH (P51414) 60S ribosomal protein L26-1, complete

0.85147995

Vv_100029 62

TC6495 5

similar to UP|Q8RV42_ARATH (Q8RV42) Holocarboxylase synthetase 2 (Holocarboxylase synthetase hcs2.b), partial (62%)

-0.84794427

Tabella S2: Lista di sonde che sono risultate significative dopo analisi dopo analisi “Significant Analysis for Microarrays (analisi SAM)” effettuate comparando polpa di controllo e polpa trattata con CO₂. “Oligo ID” è il codice assegnato ad ogni sonda (Operon). “TC (v5.0 TIGR)” si riferisce alla sequenza Tentative Consensus (TC) o al singolo ES, nel sito web TIGR (http://compbio.dfci.harvard.edu/tgi/), da cui gli oligo sono stati selezionati. “Description” è il nome assegnato ad ogni TC, come descrivono Rotter et al., (BMC Plant Biology 2009, 9:104). “BINCODE”

corrisponde al BIN ed al subBIN (http://www.gabipd.org/projects/MapMan/) assegnati ad ogni Vitis vinifera TC presenti nel set di dati AROS1. “BINNAME” è la descrizione assegnata ad ogni BIN e ad ogni SubBIN. I valori

(20)

riportano i risultati delle analisi microarray, espressi come log2, comparando la tesi di controllo con quella trattata con CO2.

oligo ID TC (v 5.0 TIGR)

description value BINCODE BINNAME

Vv_10003 860

TC65677 homologue to UP|CB21_GOSHI (P27518) Chlorophyll a-b binding protein 151, chloroplast precursor (LHCII type II CAB-151) (LHCP), partial (81%)

1.2448662 '1.1.1.1' 'PS.lightreaction.phot osystem II.LHC-II'

Vv_10007 676

CD0124 30

similar to

SP|P27788|FER3_MAIZE Ferredoxin III chloroplast precursor (Fd III). [Maize] {Zea mays}, partial (46%)

0.999807965 '1.1.5.2' 'PS.lightreaction.othe r electron carrier (ox/red).ferredoxin'

Vv_10007 527

CB98300 2

similar to

GP|15146208|gb|AAK83587.1 AT4g26270/T25K17_80 {Arabidopsis thaliana}, partial (9%)

0.844319975 '4.4' 'glycolysis.PPFK'

Vv_10006 489

CB34901 0

similar to

SP|P48502|UCR6_SOLTU Ubiquinol-cytochrome C reductase complex 14 kDa protein (EC 1.10.2.2) (CR14). [Potato], partial (78%)

1.2874466 '9.5' 'mitochondrial electron transport / ATP

synthesis.cytochrome c reductase'

Vv_10011 127

TC62438 similar to UP|Q8VWN8_GOSHI (Q8VWN8) Reversibly

glycosylated polypeptide, partial (32%)

-0.87041707 '10.5.5' 'cell wall.cell wall proteins.RGP'

Vv_10006 744

DT02075 7

'MULTIPLE HITS: 2 |

DT020757;TC64309 | DT020757:

AND TC64309: weakly similar to UP|Q9SMT3_ARATH (Q9SMT3) Endo-polygalacturonase-like protein (Glycoside hydrolase family 28 protein), partial (27%)'

-1.0692195 '10.6.3' 'cell

wall.degradation.pect ate lyases and polygalacturonases'

Vv_10006 906

TC63104 similar to

RF|NP_178161.1|15220142|NM_10 6694 NHO1; carbohydrate kinase {Arabidopsis thaliana} (exp=-1;

wgp=0; cg=0) , partial (46%)

-0.9518074 '11.5.1' 'lipid

metabolism.glyceral metabolism.glycerol kinase'

Vv_10000 146

TC51748 homologue to UP|METL_CATRO (Q96552) S-adenosylmethionine synthetase 2 (Methionine adenosyltransferase 2) (AdoMet synthetase 2) , complete

-1.04297985 '13.1.3.4' 'amino acid

aspartate

family.methionine'

Vv_10001 308

TC53454 similar to

GB|AAP68293.1|31711874|BT008 854 At1g80360 {Arabidopsis thaliana} (exp=-1; wgp=0; cg=0), partial (42%)

-0.88235414 '13.1.6.2' 'amino acid

aromatic

aa.phenylalanine and tyrosine'

Vv_10002 984

TC67256 similar to

-1.45521045 '13.1.6.2' 'amino acid

aromatic

(21)

Vv_10011 573

TC53454 similar to

-1.1451991 '13.1.6.2' 'amino acid

aromatic

Vv_10007 288

TC61278 homologue to UP|SAHH1_ARATH (O23255) Adenosylhomocysteinase 1 (S-adenosyl-L-homocysteine hydrolase 1) (SAH hydrolase 1) (AdoHcyase 1) (HOMOLOGY- DEPENDENT GENE SILENCING 1 protein) , complete

-0.977824 '13.2.3.4' 'amino acid

metabolism.degradati on.aspartate

family.methionine'

Vv_10004 842

TC52651 L-idonate dehydrogenase [Vitis vinifera]

0.8053254 '16.2.1.10' 'secondary

metabolism.phenylpr opanoids.lignin biosynthesis.CAD' Vv_10004

020

TC60430 weakly similar to

UP|Q84RC3_NICSY (Q84RC3) Gibberellin 2-oxidase 1, partial (15%)

-1.54407005 '17.5.1' 'hormone

132

RF|XP_476309.1|50933563|XM_47 6309 ethylene-forming-enzyme-like dioxygenase-like protein {Oryza sativa (japonica cultivar-group)}

0.80221943 '17.5.1' 'hormone

synthesis- degradation'

Vv_10011 205

CB34657 7

weakly similar to

GP|19225065|gb|AAL32037.2 ethylene-responsive transciptional coactivator-like protein {Retama raetam}, partial (49%)

0.804341 '17.5.3' 'hormone

metabolism.ethylene.i nduced-regulated- responsive-activated' Vv_10010

885

TC56327 homologue to UP|P93621_VITVI (P93621) Osmotin-like protein precursor, partial (56%)

-0.88537395 '20.1' 'stress.biotic'

Vv_10003 682

TC63472 similar to UP|Q39929_HELAN (Q39929) 18.6 kDa heat-shock protein, partial (92%)

1.18377175 '20.2.1' 'stress.abiotic.heat'

Vv_10000 476

TC65753 S-adenosyl-L-methionine decarboxylase [Vitis vinifera]

-0.8331654 '22.1.2' 'polyamine

SAM decarboxylase' Vv_10008

575

UP|P93569_SOLTU (P93569) Sts15 protein, partial (46%)

-0.90331398 '26.18' 'misc.invertase/pectin methylesterase inhibitor family protein' Vv_10001

161

CB91895 9

'MULTIPLE HITS: 3 |

CB918959;TC57749;TC65187 | CB918959: AND TC57749:

similar to UP|Q8S9L4_ARATH (Q8S9L4) At2g43020/MFL8.12, partial (23%) AND TC65187:

similar to UP|Q8S9L4_ARATH (Q8S9L4) At2g43020/MFL8.12, partial (28%)'

-0.94964775 '26.7' 'misc.oxidases - copper, flavone etc.'

Vv_10000 081

CB34425 8

'MULTIPLE HITS: 2 |

CB344258;TC59175 | CB344258:

AND TC59175:

UP|Q8M8U0_VITVI (Q8M8U0) Maturase (Fragment), complete'

-1.0488976 '27.1.1' 'RNA.processing.spli cing'

(22)

Vv_10008 786

TC62054 similar to

RF|NP_190012.1|15229916|NM_11 4294 ribonuclease/ transcriptional repressor {Arabidopsis thaliana}

0.839048595 '27.1.19' 'RNA.processing.ribo nucleases'

Vv_10004 205

TC60322 similar to UP|Q52QR2_SOYBN (Q52QR2) NAC domain protein NAC4, partial (66%)

-0.85766995 '27.3.27' 'RNA.regulation of transcription.NAC domain transcription factor family' Vv_10000

588

TC52586 similar to UP|H1_LYCES (P37218) Histone H1, partial (35%)

-0.85078055 '28.1.3' 'DNA.synthesis/chro matin

structure.histone' Vv_10009

324

TC64405 homologue to

UP|Q2XPW1_SOLTU (Q2XPW1) Histone H2B-like protein, partial (95%)

-0.86459558 '28.1.3' 'DNA.synthesis/chro matin

structure.histone' Vv_10004

629

RF|XP_506503.1|51963598|XM_50 6503 OJ1340_C08.131 gene product {Oryza sativa (japonica cultivar-group)} (exp=-1; wgp=0;

cg=0), partial (50%)

-0.878204635 '29.2.3' 'protein.synthesis.initi ation'

Vv_10006 701

CB91303 2

null -0.84293905 '29.4' 'protein.postranslatio

nal modification' Vv_10000

335

TC58501 homologue to UP|O22656_MAIZE (O22656) Ubiquitin-conjugating enzyme protein E2, partial (92%)

0.94424045 '29.5.11.3' 'protein.degradation.u biquitin.E2'

Vv_10003 436

UP|CBP23_HORVU (P52711) Serine carboxypeptidase II-3 precursor (CP-MII.3) [Contains:

Serine carboxypeptidase II-3 chain A; Serine carboxypeptidase II-3 chain B] , partial (42%)

0.85836555 '29.5.5' 'protein.degradation.s erine protease'

Vv_10003 793

TC57393 homologue to UP|YCF2_TOBAC (P09976) Protein ycf2, complete

-0.95140922 '29.8' 'protein assembly and cofactor ligation' Vv_10000

395

TC53615 similar to UP|ALLB3_BETVE (P43187) Calcium-binding allergen Bet v 3 (Bet v III), partial (80%)

0.8762677 '30.3' 'signalling.calcium'

Vv_10003 589

TC63536 similar to

RF|NP_176021.1|15223490|NM_10 4504 ATPP2-B14 {Arabidopsis thaliana} (exp=-1; wgp=0; cg=0), partial (31%)

1.05963195 '31.1' 'cell.organisation'

Vv_10003 987

TC65196 similar to

RF|NP_175635.1|15218189|NM_10 4104 microtubule motor

-0.933740425 '31.1' 'cell.organisation'

Vv_10010 857

UP|Q84TL6_MAIZE (Q84TL6) Legumin-like protein, partial (34%)

(23)

Vv_10000 050

BQ7956 58

'MULTIPLE HITS: 3 |

BQ795658;TC68767;TC60678 | BQ795658: AND TC68767:

homologue to UP|O04892_TOBAC (O04892) Cytochrome P450 like_TBP , partial (21%) AND TC60678: similar to

UP|Q84XC6_9ROSI (Q84XC6) Plasma intrinsic protein 2,2, complete'

-0.907931 '34.19.1' 'transport.Major Intrinsic Proteins.PIP'

Vv_10011 100

UP|Q8W1A8_PETHY (Q8W1A8) Aquaporin-like protein, partial (34%)

1.36168325 '34.19.1' 'transport.Major Intrinsic Proteins.PIP'

Vv_10006 086

CB34201 0

similar to PIR|T50691|T50691 amino acid permease 6 [imported] - Arabidopsis thaliana, partial (20%)

-0.9804903 '34.3' 'transport.amino acids'

Vv_10002 500

TC59474 similar to UP|O81977_SOYBN (O81977) Rudimentary enhancer (Fragment), partial (84%)

Vv_10003 907

TC69253 similar to

RF|NP_564756.1|18406743|NM_10 4729 electron transporter

0.973705575 '35.1' 'not assigned.no ontology'

Vv_10006 472

CB34856 1

'MULTIPLE HITS: 3 |

CB348561;TC67148;CB348476 | CB348561: AND TC67148:

similar to UP|Q9LIR4_ARATH (Q9LIR4) Dihydroxy-acid dehydratase

(AT3g23940/F14O13_13), partial (53%) AND CB348476:'

0.8460814 '35.1' 'not assigned.no ontology'

Vv_10011 941

TC59079 similar to UP|Q9FKE8_ARATH (Q9FKE8) Arabidopsis thaliana genomic DNA, chromosome 5, TAC clone:K18C1, partial (10%)

Vv_10000 052

TC65105 similar to UP|Q9M435_QUERO (Q9M435) Phase-change related protein precursor, partial (68%)

-0.9040269 '35.2' 'not

assigned.unknown'

Vv_10000 076

UP|O64594_ARATH (O64594) F17O7.4 (At1g70420/F17O7_4), partial (12%)

-0.84483065 '35.2' 'not

assigned.unknown'

Vv_10000 699

TC52003 UP|Q2TE89_9VIRU (Q2TE89) Coat protein, partial (84%)

1.0223779 '35.2' 'not

928

TC67003 similar to UP|Q9SI74_ARATH (Q9SI74) F23N19.12, partial (6%)

1.011074 '35.2' 'not

648

UP|Q94JH8_ORYSA (Q94JH8) Cold induced protein-like, partial (28%)

1.08351528 '35.2' 'not

assigned.unknown'

Vv_10003 030

TC63431 UP|Q6YCG3_VITVI (Q6YCG3) Wound induced protein-like

0.9181352 '35.2' 'not

assigned.unknown'

(24)

(Fragment), complete

Vv_10003 726

TC69654 null -0.90695042 '35.2' 'not

570

TC69592 similar to

GB|AAQ89647.1|37202064|BT010 625 At1g75810 {Arabidopsis thaliana} (exp=-1; wgp=0; cg=0), partial (74%)

1.081512775 '35.2' 'not

assigned.unknown'

Vv_10008 226

CF21002 9

weakly similar to

GP|1834392|emb|CAA70821.1 Tig {Bacillus subtilis}, partial (10%)

-2.07300565 '35.2' 'not

463

TC67003 similar to UP|Q9SI74_ARATH (Q9SI74) F23N19.12, partial (6%)

0.85725885 '35.2' 'not

806

TC68457 similar to

GB|AAX21764.1|60499009|AY929 158 prolactin {Acanthopagrus schlegelii} (exp=-1; wgp=0; cg=0), partial (7%)

1.56372425 '35.2' 'not

assigned.unknown'

Vv_10012 171

TC67295 similar to UP|Q337D0_ORYSA (Q337D0) Expressed protein, partial (94%)

-0.8807082 '35.2' 'not

319

TC70738 null -0.82173162 '35.2' 'not

460

TC67659 null 0.962782375 '35.2' 'not

779

CF60693 6

'MULTIPLE HITS: 2 |

CF606936;TC66784 | CF606936:

AND TC66784:'

0.8245948 '35.2' 'not

391

CA8107 75

'MULTIPLE HITS: 2 |

CA810775;TC62993 | CA810775:

AND TC62993:

gb|M75722.1|ALSCP23SA Alnus incana chloroplast 23S ribosomal RNA (23S rRNA) gene, partial (22%)'

0.88106532 '35.2' 'not

assigned.unknown'

Vv_10013 752

TC57722 null -0.82891985 '35.2' 'not

466

TC61729 similar to UP|Q3G892_9DELT (Q3G892) Cytochrome c assembly protein precursor, partial (6%)

-0.82528555 '35.2' 'not

assigned.unknown'

Vv_10000 002

TC64995 similar to UP|Q9XGN4_AJURE (Q9XGN4) Galactinol synthase, isoform GolS-1 , partial (46%)

Vv_10000 123

TC63888 homologue to UP|Q4VT47_VITVI (Q4VT47) RD22-like protein, partial (39%)

(25)

Vv_10000 156

CF60572 9

'MULTIPLE HITS: 11 |

CF605729;TC52641;CB973647;CB 970967;TC67765;TC60662;TC589 98;TC56657;TC56459;TC52498;D V940553 | CF605729: AND TC52641: homologue to

GB|AAK95257.1|15294160|AF410 271 At1g13440/F13B4_8

wgp=0; cg=0), partial (49%) AND CB973647: AND CB970967:

AND TC67765: similar to UP|G3PC_MAGLI (P26518) Glyceraldehyde-3-phosphate dehydrogenase, cytosolic , partial (52%) AND TC60662: homologue to UP|G3PC_MAGLI (P26518) Glyceraldehyde-3-phosphate dehydrogenase, cytosolic , partial (37%) AND TC58998: homologue to UP|G3PC_MAGLI (P26518) Glyceraldehyde-3-phosphate dehydrogenase, cytosolic , partial (35%) AND TC56657: homologue to

GB|AAK95257.1|15294160|AF410 271 At1g13440/F13B4_8

wgp=0; cg=0), partial (85%) AND TC56459: homologue to

GB|AAK95257.1|15294160|AF410 271 At1g13440/F13B4_8

wgp=0; cg=0), partial (98%) AND TC52498: homologue to

GB|CAA42103.1|1345501|AMGA DPH glycolytic glyceraldehyde 3- phosphate dehydrogenase {Antirrhinum majus} (exp=-1;

wgp=0; cg=0), partial (65%) AND DV940553:'

Vv_10000 176

TC71063 'MULTIPLE HITS: 5 |

TC71063;TC68949;TC58902;AJ86 3085;CB339196 | TC71063: AND TC68949: UP|Q9FS43_VITVI (Q9FS43) Pathogenesis-related protein 10, complete AND TC58902: UP|Q9FS43_VITVI (Q9FS43) Pathogenesis-related protein 10, complete AND AJ863085: AND CB339196:'

Vv_10000 183

CF60602 0

'MULTIPLE HITS: 3 |

CF606020;TC53837;CB347041 | CF606020: AND TC53837: similar to UP|Q52QX4_MANES

(Q52QX4) Auxin-repressed protein-like protein ARP1, partial (91%) AND CB347041:'

(26)

Vv_10003 664

TC65732 similar to UP|Q2XPV1_SOLTU (Q2XPV1) Drm3-like protein-like protein, partial (41%)

Vv_10004 039

TC67843 similar to UP|Q76LM3_9ROSI (Q76LM3) Hydroperoxide lyase, partial (43%)

Vv_10004 099

TC58996 similar to UP|Q9LE80_ARATH (Q9LE80) Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone: MJK13

(AT3g15450/MJK13_11)

(MJK13.11 protein), partial (94%)

Vv_10004 111

TC56465 'MULTIPLE HITS: 3 | TC56465;TC52861;TC52469 | TC56465: AND TC52861: weakly similar to UP|Q9LKW3_LYCES (Q9LKW3) Dehydration-induced protein ERD15, partial (72%) AND TC52469: similar to

UP|Q8LBP5_ARATH (Q8LBP5) ERD15 protein, partial (42%)'

Vv_10004 584

UP|Q6ZHE6_ORYSA (Q6ZHE6) Universal stress protein / early nodulin ENOD18-like, partial (56%)

Vv_10006 699

CB91285 8

similar to

GP|17225194|gb|AAL37293.1 sorbitol dehydrogenase {Malus x domestica}, partial (13%)

Vv_10009 699

TC67523 homologue to UP|VATB2_GOSHI (Q43433) Vacuolar ATP synthase subunit B isoform 2 (V-ATPase B subunit 2) (Vacuolar proton pump B subunit 2) (Fragment) , partial (92%)

Vv_10011 346

DY4741 43

'MULTIPLE HITS: 3 |

DY474143;TC58885;DV940625 | DY474143: AND TC58885:

homologue to UP|GME1_ORYSA (Q338B5) GDP-mannose 3,5- epimerase 1 (GDP-Man 3,5- epimerase 1) (OsGME-1) , partial (98%) AND DV940625:'