E’ stata eseguita un’elettroforesi in tricina-SDS-PAGE delle frazioni 2, 3, 4 e 5, per separare le proteine da 26.6 kDa a 1.4 kDa. Come si può vedere in Figura 37, nella frazione 2 è presente una banda a 26.6 kDa dovuta a proteine ad alto peso molecolare, tuttavia sono presenti bande fino a un peso molecolare di circa 6.5 kDa; nelle frazioni 3 e 4 invece si ha una diminuzione di intensità delle bande presenti nella frazione 2, ma si ha un arricchimento di proteine con pesi molecolari compresi tra 6.5 kDa e 3.5 kDa. Va
74 sottolineato che, la precipitazione con TCA al 20%, non consente un recupero del 100% delle proteine presenti in ogni frazione, ma il recupero sarà circa del 70%.
Come atteso, invece, la frazione 5 non mostra alcuna banda, infatti in essa dovevano essere contenuti peptidi a basso peso molecolare, inferiori a 1.4 kDa, che non è possibile separare con questa tecnica.
75
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81
Ringraziamenti
Desidero ringraziare il Professor Antonio Lucacchini e la Dottoressa Laura Giusti per avermi dato la possibilità di approfondire questa interessante tematica e per avermi aiutata e indirizzata ogni qualvolta ne avessi avuto bisogno.
Grazie ad Isabella che, in qualità di tutor, mi ha guidata durante lo svolgimento del lavoro in laboratorio e nella stesura della tesi con grande disponibilità e impegno, donandomi preziosi consigli.
Un grazie a Silvia, per questi anni di studio condivisi insieme tra schemi e registrazioni, ma soprattutto per la nostra amicizia (da cinque anni a questa parte mi sento anche un po’ varesotta!).
Grazie a tutte le mie amiche compagne di corso Giulia, Sara, Nunzia, Caterina, Federica, Lorenza, Ilaria, Laura e Silvia C.
Un grazie affettuoso a Federica e Giuliano e a tutto lo staff della farmacia, dove ho svolto tirocinio, per i loro insegnamenti, consigli e per avermi fatta sentire a casa. Un grazie di cuore ai miei genitori Gabriella e Antonello, alla nonna Franca e al mio ragazzo Tommaso, che mi hanno sempre sostenuta, supportata e sopportata anche nei peggiori momenti di ansia.