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- + - - anti-RelA/p65 Ab

- - + - unlabeled NF-kB probe

- - - + unlabeled mtNF-kB probe

protein-DNA complexes

free probe

Supplementary Figure 2. Nuclear extracts (2 µg) of SIE cells treated for 20 minutes with

50 ng/mL rDrm were incubated with a biotin-labeled NF-kB double-stranded DNA

oligonucleotide probe (5’AGTTGAGGGGACTTTCCCAGGC) in the absence or in the

presence of anti-RelA/p65 antibodies or of a molar excess of the unlabeled NF-kB probe

or of a mutant NF-kB probe (5’AGTTGAGGCGACTTTCCCAGGC). The protein-DNA

complexes were analyzed by EMSA onto a native 6% polyacrylamide gel.

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