Ruolo del sistema fitocromo nell'accumulo di flavonoidi nel frutto di mutanti di pomodoro

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APPENDICE

1.1 Fotografie

cv. MM

phyA

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phyA/phyB1

phyA/phyB2

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cv. MM

phyA

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phyB2

phyA/phyB1

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hp-1

ANOVA e Tukey’s test

Parameter Value B1 B2 AB1

Table Analyzed

QUERCETINA BUCCIA

One-way analysis of variance

P value P<0.0001 P value summary *** Are means signif. different? (P < 0.05) Yes Number of groups 9

F 1528

R squared 0,9985

ANOVA Table SS df MS

Treatment (between columns) 294000 8 36750 Residual (within columns) 433 18 24,05

Total 294400 26

Tukey's Multiple Comparison Test Mean Diff. q P value 95% CI of diff A vs B1 49,28 17,4 P < 0.001 35.25 to 63.31 A vs B2 79,07 27,92 P < 0.001 65.04 to 93.11 A vs AB1 80,77 28,53 P < 0.001 66.74 to 94.81 A vs AB2 107,5 37,96 P < 0.001 93.47 to 121.5 A vs B1B2 35,93 12,69 P < 0.001 21.90 to 49.97 A vs AB1B2 42,07 14,86 P < 0.001 28.03 to 56.10 A vs HP -264,9 93,55 P < 0.001 -278.9 to -250.9 A vs MM 27,43 9,688 P < 0.001 13.40 to 41.47 B1 vs B2 29,79 10,52 P < 0.001 15.76 to 43.83 B1 vs AB1 31,49 11,12 P < 0.001 17.46 to 45.53 B1 vs AB2 58,22 20,56 P < 0.001 44.19 to 72.25 B1 vs B1B2 -13,35 4,713 P > 0.05 -27.38 to 0.6870 B1 vs AB1B2 -7,213 2,547 P > 0.05 -21.25 to 6.820 B1 vs HP -314,2 111 P < 0.001 -328.2 to -300.1 B1 vs MM -21,85 7,715 P < 0.001 -35.88 to -7.813 B2 vs AB1 1,7 0,6004 P > 0.05 -12.33 to 15.73 B2 vs AB2 28,43 10,04 P < 0.001 14.39 to 42.46 B2 vs B1B2 -43,14 15,23 P < 0.001 -57.17 to -29.11 B2 vs AB1B2 -37,01 13,07 P < 0.001 -51.04 to -22.97 B2 vs HP -344 121,5 P < 0.001 -358.0 to -329.9

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B2 vs MM -51,64 18,24 P < 0.001 -65.67 to -37.61 AB1 vs AB2 26,73 9,439 P < 0.001 12.69 to 40.76 AB1 vs B1B2 -44,84 15,84 P < 0.001 -58.87 to -30.81 AB1 vs AB1B2 -38,71 13,67 P < 0.001 -52.74 to -24.67 AB1 vs HP -345,7 122,1 P < 0.001 -359.7 to -331.6 AB1 vs MM -53,34 18,84 P < 0.001 -67.37 to -39.31 AB2 vs B1B2 -71,57 25,27 P < 0.001 -85.60 to -57.53 AB2 vs AB1B2 -65,43 23,11 P < 0.001 -79.47 to -51.40 AB2 vs HP -372,4 131,5 P < 0.001 -386.4 to -358.4 AB2 vs MM -80,07 28,28 P < 0.001 -94.10 to -66.03 B1B2 vs AB1B2 6,133 2,166 P > 0.05 -7.900 to 20.17 B1B2 vs HP -300,8 106,2 P < 0.001 -314.9 to -286.8 B1B2 vs MM -8,5 3,002 P > 0.05 -22.53 to 5.534 AB1B2 vs HP -307 108,4 P < 0.001 -321.0 to -292.9 AB1B2 vs MM -14,63 5,168 P < 0.05 -28.67 to -0.5997 HP vs MM 292,3 103,2 P < 0.001 278.3 to 306.4

Table Analyzed

RUTINA BUCCIA

F 1543

R squared 0,9985

Treatment (between columns) 274400 8 34300 Residual (within columns) 400 18 22,22

Total 274800 26

Tukey's Multiple Comparison Test Mean Diff. q P value 95% CI of diff A vs B1 90,71 33,33 P < 0.001 77.22 to 104.2 A vs B2 146,6 53,86 P < 0.001 133.1 to 160.1 A vs AB1 113,2 41,6 P < 0.001 99.75 to 126.7 A vs AB2 169,6 62,32 P < 0.001 156.1 to 183.1 A vs B1B2 80,27 29,49 P < 0.001 66.78 to 93.76 A vs AB1B2 87,06 31,99 P < 0.001 73.57 to 100.5 A vs HP -191,4 70,33 P < 0.001 -204.9 to -177.9 A vs MM 45,62 16,76 P < 0.001 32.13 to 59.11 B1 vs B2 55,89 20,53 P < 0.001 42.40 to 69.38 B1 vs AB1 22,53 8,277 P < 0.001 9.039 to 36.02 B1 vs AB2 78,92 29 P < 0.001 65.43 to 92.41 B1 vs B1B2 -10,45 3,838 P > 0.05 -23.94 to 3.043 B1 vs AB1B2 -3,654 1,342 P > 0.05 -17.14 to 9.836 B1 vs HP -282,1 103,7 P < 0.001 -295.6 to -268.6 B1 vs MM -45,09 16,57 P < 0.001 -58.58 to -31.60 B2 vs AB1 -33,36 12,26 P < 0.001 -46.85 to -19.87 B2 vs AB2 23,03 8,461 P < 0.001 9.539 to 36.52 B2 vs B1B2 -66,34 24,37 P < 0.001 -79.83 to -52.85 B2 vs AB1B2 -59,54 21,88 P < 0.001 -73.03 to -46.06 B2 vs HP -338 124,2 P < 0.001 -351.5 to -324.5 B2 vs MM -101 37,1 P < 0.001 -114.5 to -87.49 AB1 vs AB2 56,39 20,72 P < 0.001 42.90 to 69.88 AB1 vs B1B2 -32,97 12,12 P < 0.001 -46.46 to -19.49

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AB1 vs AB1B2 -26,18 9,619 P < 0.001 -39.67 to -12.69 AB1 vs HP -304,7 111,9 P < 0.001 -318.1 to -291.2 AB1 vs MM -67,62 24,84 P < 0.001 -81.11 to -54.13 AB2 vs B1B2 -89,37 32,83 P < 0.001 -102.9 to -75.88 AB2 vs AB1B2 -82,57 30,34 P < 0.001 -96.06 to -69.08 AB2 vs HP -361 132,7 P < 0.001 -374.5 to -347.6 AB2 vs MM -124 45,56 P < 0.001 -137.5 to -110.5 B1B2 vs AB1B2 6,793 2,496 P > 0.05 -6.696 to 20.28 B1B2 vs HP -271,7 99,82 P < 0.001 -285.2 to -258.2 B1B2 vs MM -34,64 12,73 P < 0.001 -48.13 to -21.16 AB1B2 vs HP -278,5 102,3 P < 0.001 -292.0 to -265.0 AB1B2 vs MM -41,44 15,22 P < 0.001 -54.93 to -27.95 HP vs MM 237 87,09 P < 0.001 223.5 to 250.5

Table Analyzed

CAMFEROLO BUCCIA

F 203,1

R squared 0,989

Treatment (between columns) 506,9 8 63,36 Residual (within columns) 5,617 18 0,3121

Total 512,5 26

Tukey's Multiple Comparison Test Mean Diff. q P value 95% CI of diff A vs B1 -2,04 6,325 P < 0.01 -3.638 to -0.4416 A vs B2 0,51 1,581 P > 0.05 -1.088 to 2.108 A vs AB1 0,86 2,667 P > 0.05 -0.7384 to 2.458 A vs AB2 2,097 6,501 P < 0.01 0.4983 to 3.695 A vs B1B2 -0,4067 1,261 P > 0.05 -2.005 to 1.192 A vs AB1B2 2,383 7,39 P < 0.01 0.7849 to 3.982 A vs HP -12,83 39,77 P < 0.001 -14.43 to -11.23 A vs MM -1,577 4,889 P > 0.05 -3.175 to 0.02173 B1 vs B2 2,55 7,907 P < 0.001 0.9516 to 4.148 B1 vs AB1 2,9 8,992 P < 0.001 1.302 to 4.498 B1 vs AB2 4,137 12,83 P < 0.001 2.538 to 5.735 B1 vs B1B2 1,633 5,064 P < 0.05 0.03494 to 3.232 B1 vs AB1B2 4,423 13,72 P < 0.001 2.825 to 6.022 B1 vs HP -10,79 33,45 P < 0.001 -12.39 to -9.188 B1 vs MM 0,4633 1,437 P > 0.05 -1.135 to 2.062 B2 vs AB1 0,35 1,085 P > 0.05 -1.248 to 1.948 B2 vs AB2 1,587 4,92 P > 0.05 -0.01173 to 3.185 B2 vs B1B2 -0,9167 2,842 P > 0.05 -2.515 to 0.6817 B2 vs AB1B2 1,873 5,808 P < 0.05 0.2749 to 3.472 B2 vs HP -13,34 41,35 P < 0.001 -14.94 to -11.74 B2 vs MM -2,087 6,47 P < 0.01 -3.685 to -0.4883 AB1 vs AB2 1,237 3,834 P > 0.05 -0.3617 to 2.835 AB1 vs B1B2 -1,267 3,927 P > 0.05 -2.865 to 0.3317

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AB1 vs AB1B2 1,523 4,723 P > 0.05 -0.07506 to 3.122 AB1 vs HP -13,69 42,44 P < 0.001 -15.29 to -12.09 AB1 vs MM -2,437 7,555 P < 0.01 -4.035 to -0.8383 AB2 vs B1B2 -2,503 7,762 P < 0.001 -4.102 to -0.9049 AB2 vs AB1B2 0,2867 0,8888 P > 0.05 -1.312 to 1.885 AB2 vs HP -14,92 46,27 P < 0.001 -16.52 to -13.32 AB2 vs MM -3,673 11,39 P < 0.001 -5.272 to -2.075 B1B2 vs AB1B2 2,79 8,651 P < 0.001 1.192 to 4.388 B1B2 vs HP -12,42 38,51 P < 0.001 -14.02 to -10.82 B1B2 vs MM -1,17 3,628 P > 0.05 -2.768 to 0.4284 AB1B2 vs HP -15,21 47,16 P < 0.001 -16.81 to -13.61 AB1B2 vs MM -3,96 12,28 P < 0.001 -5.558 to -2.362 HP vs MM 11,25 34,88 P < 0.001 9.652 to 12.85

Table Analyzed

NARINGENINA BUCCIA

F 451,5

R squared 0,995

Treatment (between columns) 198300 8 24790 Residual (within columns) 988,3 18 54,91

Total 199300 26

Tukey's Multiple Comparison Test Mean Diff. q P value 95% CI of diff A vs B1 -11,07 2,587 P > 0.05 -32.27 to 10.14 A vs B2 -85,77 20,05 P < 0.001 -107.0 to -64.57 A vs AB1 75,25 17,59 P < 0.001 54.05 to 96.45 A vs AB2 30,93 7,23 P < 0.01 9.728 to 52.13 A vs B1B2 67,42 15,76 P < 0.001 46.21 to 88.62 A vs AB1B2 97,98 22,9 P < 0.001 76.78 to 119.2 A vs HP -161,1 37,67 P < 0.001 -182.3 to -139.9 A vs MM -114,1 26,66 P < 0.001 -135.3 to -92.86 B1 vs B2 -74,71 17,46 P < 0.001 -95.91 to -53.50 B1 vs AB1 86,32 20,18 P < 0.001 65.11 to 107.5 B1 vs AB2 42 9,817 P < 0.001 20.79 to 63.20 B1 vs B1B2 78,48 18,35 P < 0.001 57.28 to 99.69 B1 vs AB1B2 109,1 25,49 P < 0.001 87.85 to 130.3 B1 vs HP -150,1 35,08 P < 0.001 -171.3 to -128.9 B1 vs MM -103 24,08 P < 0.001 -124.2 to -81.79 B2 vs AB1 161 37,64 P < 0.001 139.8 to 182.2 B2 vs AB2 116,7 27,28 P < 0.001 95.50 to 137.9 B2 vs B1B2 153,2 35,81 P < 0.001 132.0 to 174.4 B2 vs AB1B2 183,8 42,95 P < 0.001 162.6 to 205.0 B2 vs HP -75,37 17,62 P < 0.001 -96.58 to -54.17 B2 vs MM -28,29 6,613 P < 0.01 -49.49 to -7.088 AB1 vs AB2 -44,32 10,36 P < 0.001 -65.52 to -23.12 AB1 vs B1B2 -7,833 1,831 P > 0.05 -29.04 to 13.37

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AB1 vs AB1B2 22,73 5,314 P < 0.05 1.531 to 43.94 AB1 vs HP -236,4 55,26 P < 0.001 -257.6 to -215.2 AB1 vs MM -189,3 44,25 P < 0.001 -210.5 to -168.1 AB2 vs B1B2 36,49 8,529 P < 0.001 15.28 to 57.69 AB2 vs AB1B2 67,05 15,67 P < 0.001 45.85 to 88.26 AB2 vs HP -192,1 44,9 P < 0.001 -213.3 to -170.9 AB2 vs MM -145 33,89 P < 0.001 -166.2 to -123.8 B1B2 vs AB1B2 30,57 7,145 P < 0.01 9.365 to 51.77 B1B2 vs HP -228,6 53,43 P < 0.001 -249.8 to -207.4 B1B2 vs MM -181,5 42,42 P < 0.001 -202.7 to -160.3 AB1B2 vs HP -259,1 60,57 P < 0.001 -280.3 to -237.9 AB1B2 vs MM -212 49,57 P < 0.001 -233.2 to -190.8 HP vs MM 47,08 11,01 P < 0.001 25.88 to 68.29

Table Analyzed

QUERCETINA POLPA

F 71,15

R squared 0,9693

Treatment (between columns) 1054 8 131,7 Residual (within columns) 33,32 18 1,851

Total 1087 26

Tukey's Multiple Comparison Test Mean Diff. q P value 95% CI of diff A vs B1 -6,38 8,122 P < 0.001 -10.27 to -2.487 A vs B2 -8,147 10,37 P < 0.001 -12.04 to -4.254 A vs AB1 4,287 5,457 P < 0.05 0.3937 to 8.180 A vs AB2 5,303 6,751 P < 0.01 1.410 to 9.196 A vs B1B2 5,787 7,367 P < 0.01 1.894 to 9.680 A vs AB1B2 5,773 7,35 P < 0.01 1.880 to 9.666 A vs HP -1,533 1,952 P > 0.05 -5.426 to 2.360 A vs MM -11,54 14,69 P < 0.001 -15.43 to -7.644 B1 vs B2 -1,767 2,249 P > 0.05 -5.660 to 2.126 B1 vs AB1 10,67 13,58 P < 0.001 6.774 to 14.56 B1 vs AB2 11,68 14,87 P < 0.001 7.790 to 15.58 B1 vs B1B2 12,17 15,49 P < 0.001 8.274 to 16.06 B1 vs AB1B2 12,15 15,47 P < 0.001 8.260 to 16.05 B1 vs HP 4,847 6,17 P < 0.01 0.9537 to 8.740 B1 vs MM -5,157 6,565 P < 0.01 -9.050 to -1.264 B2 vs AB1 12,43 15,83 P < 0.001 8.540 to 16.33 B2 vs AB2 13,45 17,12 P < 0.001 9.557 to 17.34 B2 vs B1B2 13,93 17,74 P < 0.001 10.04 to 17.83 B2 vs AB1B2 13,92 17,72 P < 0.001 10.03 to 17.81 B2 vs HP 6,613 8,419 P < 0.001 2.720 to 10.51 B2 vs MM -3,39 4,316 P > 0.05 -7.283 to 0.5030 AB1 vs AB2 1,017 1,294 P > 0.05 -2.876 to 4.910 AB1 vs B1B2 1,5 1,91 P > 0.05 -2.393 to 5.393

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AB1 vs AB1B2 1,487 1,893 P > 0.05 -2.406 to 5.380 AB1 vs HP -5,82 7,409 P < 0.01 -9.713 to -1.927 AB1 vs MM -15,82 20,14 P < 0.001 -19.72 to -11.93 AB2 vs B1B2 0,4833 0,6153 P > 0.05 -3.410 to 4.376 AB2 vs AB1B2 0,47 0,5983 P > 0.05 -3.423 to 4.363 AB2 vs HP -6,837 8,703 P < 0.001 -10.73 to -2.944 AB2 vs MM -16,84 21,44 P < 0.001 -20.73 to -12.95 B1B2 vs AB1B2 -0,01333 0,01697 P > 0.05 -3.906 to 3.880 B1B2 vs HP -7,32 9,319 P < 0.001 -11.21 to -3.427 B1B2 vs MM -17,32 22,05 P < 0.001 -21.22 to -13.43 AB1B2 vs HP -7,307 9,302 P < 0.001 -11.20 to -3.414 AB1B2 vs MM -17,31 22,04 P < 0.001 -21.20 to -13.42 HP vs MM -10 12,73 P < 0.001 -13.90 to -6.110

Table Analyzed

RUTINA POLPA

F 453,4

R squared 0,9951

Treatment (between columns) 815,9 8 102 Residual (within columns) 4,049 18 0,2249

Total 819,9 26

Tukey's Multiple Comparison Test Mean Diff. q P value 95% CI of diff A vs B1 2,53 9,24 P < 0.001 1.173 to 3.887 A vs B2 -1,655 6,044 P < 0.05 -3.012 to -0.2980 A vs AB1 3,583 13,09 P < 0.001 2.226 to 4.940 A vs AB2 1,187 4,334 P > 0.05 -0.1704 to 2.544 A vs B1B2 -0,21 0,7669 P > 0.05 -1.567 to 1.147 A vs AB1B2 2,565 9,368 P < 0.001 1.208 to 3.922 A vs HP -15,69 57,3 P < 0.001 -17.05 to -14.33 A vs MM -2,863 10,46 P < 0.001 -4.220 to -1.506 B1 vs B2 -4,185 15,28 P < 0.001 -5.542 to -2.828 B1 vs AB1 1,053 3,847 P > 0.05 -0.3037 to 2.410 B1 vs AB2 -1,343 4,906 P > 0.05 -2.700 to 0.01369 B1 vs B1B2 -2,74 10,01 P < 0.001 -4.097 to -1.383 B1 vs AB1B2 0,035 0,1278 P > 0.05 -1.322 to 1.392 B1 vs HP -18,22 66,54 P < 0.001 -19.58 to -16.86 B1 vs MM -5,393 19,7 P < 0.001 -6.750 to -4.036 B2 vs AB1 5,238 19,13 P < 0.001 3.881 to 6.595 B2 vs AB2 2,842 10,38 P < 0.001 1.485 to 4.199 B2 vs B1B2 1,445 5,277 P < 0.05 0.08797 to 2.802 B2 vs AB1B2 4,22 15,41 P < 0.001 2.863 to 5.577 B2 vs HP -14,03 51,25 P < 0.001 -15.39 to -12.68 B2 vs MM -1,208 4,413 P > 0.05 -2.565 to 0.1487 AB1 vs AB2 -2,397 8,753 P < 0.001 -3.754 to -1.040 AB1 vs B1B2 -3,793 13,85 P < 0.001 -5.150 to -2.436

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AB1 vs AB1B2 -1,018 3,719 P > 0.05 -2.375 to 0.3387 AB1 vs HP -19,27 70,38 P < 0.001 -20.63 to -17.91 AB1 vs MM -6,447 23,54 P < 0.001 -7.804 to -5.090 AB2 vs B1B2 -1,397 5,101 P < 0.05 -2.754 to -0.03964 AB2 vs AB1B2 1,378 5,034 P < 0.05 0.02131 to 2.735 AB2 vs HP -16,88 61,63 P < 0.001 -18.23 to -15.52 AB2 vs MM -4,05 14,79 P < 0.001 -5.407 to -2.693 B1B2 vs AB1B2 2,775 10,13 P < 0.001 1.418 to 4.132 B1B2 vs HP -15,48 56,53 P < 0.001 -16.84 to -14.12 B1B2 vs MM -2,653 9,69 P < 0.001 -4.010 to -1.296 AB1B2 vs HP -18,25 66,66 P < 0.001 -19.61 to -16.90 AB1B2 vs MM -5,428 19,82 P < 0.001 -6.785 to -4.071 HP vs MM 12,83 46,84 P < 0.001 11.47 to 14.18

Table Analyzed

NARINGENINA POLPA

F 56,26

R squared 0,9615

Treatment (between columns) 126,8 8 15,85 Residual (within columns) 5,072 18 0,2818

Total 131,9 26

Tukey's Multiple Comparison Test Mean Diff. q P value 95% CI of diff A vs B1 -0,04 0,1305 P > 0.05 -1.559 to 1.479 A vs B2 -3,29 10,73 P < 0.001 -4.809 to -1.771 A vs AB1 1,833 5,982 P < 0.05 0.3144 to 3.352 A vs AB2 2,173 7,091 P < 0.01 0.6544 to 3.692 A vs B1B2 -0,4467 1,457 P > 0.05 -1.966 to 1.072 A vs AB1B2 1,227 4,002 P > 0.05 -0.2922 to 2.746 A vs HP -4,787 15,62 P < 0.001 -6.306 to -3.268 A vs MM -0,02333 0,07613 P > 0.05 -1.542 to 1.496 B1 vs B2 -3,25 10,6 P < 0.001 -4.769 to -1.731 B1 vs AB1 1,873 6,112 P < 0.01 0.3544 to 3.392 B1 vs AB2 2,213 7,222 P < 0.01 0.6944 to 3.732 B1 vs B1B2 -0,4067 1,327 P > 0.05 -1.926 to 1.112 B1 vs AB1B2 1,267 4,133 P > 0.05 -0.2522 to 2.786 B1 vs HP -4,747 15,49 P < 0.001 -6.266 to -3.228 B1 vs MM 0,01667 0,05438 P > 0.05 -1.502 to 1.536 B2 vs AB1 5,123 16,72 P < 0.001 3.604 to 6.642 B2 vs AB2 5,463 17,83 P < 0.001 3.944 to 6.982 B2 vs B1B2 2,843 9,277 P < 0.001 1.324 to 4.362 B2 vs AB1B2 4,517 14,74 P < 0.001 2.998 to 6.036 B2 vs HP -1,497 4,883 P > 0.05 -3.016 to 0.02224 B2 vs MM 3,267 10,66 P < 0.001 1.748 to 4.786 AB1 vs AB2 0,34 1,109 P > 0.05 -1.179 to 1.859 AB1 vs B1B2 -2,28 7,439 P < 0.01 -3.799 to -0.7611

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AB1 vs AB1B2 -0,6067 1,979 P > 0.05 -2.126 to 0.9122 AB1 vs HP -6,62 21,6 P < 0.001 -8.139 to -5.101 AB1 vs MM -1,857 6,058 P < 0.05 -3.376 to -0.3378 AB2 vs B1B2 -2,62 8,549 P < 0.001 -4.139 to -1.101 AB2 vs AB1B2 -0,9467 3,089 P > 0.05 -2.466 to 0.5722 AB2 vs HP -6,96 22,71 P < 0.001 -8.479 to -5.441 AB2 vs MM -2,197 7,167 P < 0.01 -3.716 to -0.6778 B1B2 vs AB1B2 1,673 5,46 P < 0.05 0.1544 to 3.192 B1B2 vs HP -4,34 14,16 P < 0.001 -5.859 to -2.821 B1B2 vs MM 0,4233 1,381 P > 0.05 -1.096 to 1.942 AB1B2 vs HP -6,013 19,62 P < 0.001 -7.532 to -4.494 AB1B2 vs MM -1,25 4,079 P > 0.05 -2.769 to 0.2689 HP vs MM 4,763 15,54 P < 0.001 3.244 to 6.282