Concludendo, possiamo affermare che la somministrazione del Kavolì® (1g/kg p.v.) ha portato ad un miglioramento della steatosi epatica confermato dalla diminuzione del contenuto lipidico. Inoltre questa Brassica oleracea var. acephala ha dimostrato di possedere un’attività antiossidante ed antinfiammatoria a livello del fegato poiché migliora il quadro di progressione da NAFLD a NASH, caratterizzato da alti livelli di stress ossidativo, presenza di infiltrato infiammatorio e produzione di citochine pro- infiammatorie.
La somministrazione di due mesi di dieta ipelipidica non sembra provocare stress ossidativo nel colon. A livello dell’encefalo invece, si ha un forte evidenza di stress ossidativo portato dalla dieta iperlipidica e dall’iperglicemia. È noto da tempo infatti che il sistema nervoso centrale sia particolarmente vulnerabile al danno da radicali liberi, se confrontato con altri tessuti. Le proprietà benefiche dell’estratto di Kavolì® mostrano un effetto antiossidante anche in quest’organo.
7. Bibliografia
A
Abboud P. A., Hake P. W., Burroughs T. J., Odoms K., O’Connor M., Mangeshkar P., Michael O'Connor, Hector R. Wong, Zingarelli B. - Therapeutic effect of
epigallocatechin‑3‑gallate in a mouse model of colitis. Eur J Pharmacol. 2008, 579:
411‑7.
Abdelmegeed M. A., Banerjee A., Yoo S. H., Jang S., Gonzalez F. J., Song B. J. Critical
role of cytochrome P450 2E1 (CYP2E1) in the development of high fatinduced non- alcoholic steatohepatitis. J Hepatol. 2012, 57: 860-866
Aborehab N. M., Mahitab H. El Bishbishy, and Nermien E. Waly - Resistin mediates
tomato and broccoli extract effects on glucose homeostasis in high fat diet-induced obesity in rats. BMC Complement Altern Med. 2016, 16: 225.
Ahmed F. A., Ali R. F. M. - Bioactive Compounds and Antioxidant Activity of Fresh and
Processed White Cauliflower. BioMed Res Intern. 2013, 2013: 367819.
Ahn T., Kim M., Yun C. H., Chae H. J. - Functional regulation of hepatic cytochrome p450
enzymes by physicochemical properties of phospholipids in biological membranes. Curr
Protein Pept Sci. 2007, 8: 496-505.
Aghajanpour M., Nazer M. R., Obeidavi Z., Akbari M., Ezati P., Kor N. M. - Functional
foods and their role in cancer prevention and health promotion: a comprehensive review.
Am J Cancer Res. 2017, 7: 740-769.
Aitio A. - A simple and sensitive assay of 7-ethoxycoumarin deethylation. Anal Biochem 1978, 85: 488-491.
Albano E., Mottaran E., Vidali M., Reale E., Saksena S., Occhino G., Burt A. D., Day C. P. - Immune response towards lipid peroxidation products as a predictor of progression
of nonalcoholic fatty liver disease to advanced fibrosis. Gut. 2005, 54: 987-993.
Alisi A., Ceccarelli S., Panera N., Nobili V. - Causative role of gut microbiota in non-
alcoholic fatty liver disease pathogenesis. Front Cell Infect Microbiol. 2012; 2: 132 1-4.
Antonucci Ramona –Valutazione delle proprietà nutraceutiche di Brassica oleracea
var.acephala e dei suoi effetti su cellule endoteliali umane del microcircolo. Tesi 2016 -
Biologia applicata alla biomedicina.
Arab H. H., Al-Shorbagy M. Y., Abdallah D. M., Nassar N. N. - Telmisartan attenuates
colon inflammation, oxidative perturbations and apoptosis in a rat model of experimental inflammatory bowel disease. PLoS One. 2014 May 15;9(5):e97193
Arai S. - Studies on functional foods in Japan--state of the art. Biosci Biotechnol Biochem. 1996; 60: 9-15.
Arts I. C., Hollman P. C. Polyphenols and disease risk in epidemiologic studies. Am J Clin Nutr. 2005; 81:317S-325S.
B
Balmus I. M., Ciobica A., Trifan A., Stanciu C.- The Implications of Oxidative Stress and
Antioxidant Therapies in Inflammatory Bowel Disease: Clinical Aspects and Animal Models. Saudi J Gastroenterol. 2016, 22: 3-17.
Banerjee M., Robbins D., Chen T. - Targeting xenobiotic receptors PXR and CAR in
human diseases. Drug Discov Today. 2015, 20: 618-628.
Barillari J., Iori R., Broccoli M., Pozzetti L., Canistro D., Sapone A., Bonamassa B., Biagi G.L., Paolini M. - Glucoraphasatin and glucoraphenin, a redox pair of glucosinolates of
brassicaceae, differently affect metabolizing enzymes in rats. J Agric Food Chem. 2007,
55: 5505-5511.
Barba F. J., Nikmaram N., Roohinejad S., Khelfa A., Zhu Z. and Koubaa M. -
Bioavailability of Glucosinolates and Their Breakdown Products: impact of Processing.
Front Nutr. 2016, 3: 24.
Benson A. M., Hunkeler M. J., Talalay P. - Increase of NAD(P)H: quinine reductase by
dietary antioxidants: possible role in protection against carcinogenesis and toxicity. Proc
Natl Acad Sci. 1980, 77: 5216-5220.
Bheemreddy R. M., Jeffery E. H. - The metabolic fate of purified glucoraphanin in F344
rats. J Agric Food Chem. 2007, 55: 2861-2866.
Bjorkman M., Klingen I., Birch A. N., Bones A. M., Bruce T. J., Johansen T. J., Meadow R., Molmann J., Seljasen R., Smart L. E., Stewart D. - Phytochemicals of Brassicaceae in
plant protection and human health--influences of climate, environment and agronomic practice. Phytochemistry. 2011, 72: 538-56.
Bhandari S. R., Kwak J. H. - Chemical composition and antioxidant activity in different
tissues of brassica vegetables. Molecules. 2015, 20: 1228-43.
Briskey D., Heritage M., Jaskowski L. A., Peake J., Gobe G., Subramaniam V. N., Crawford D., Campbell C., Vitetta L. - Probiotics modify tight-junction proteins in an
animal model of nonalcoholic fatty liver disease. Therap Adv Gastroenterol. 2016, 9: 463-
72.
Burt A. D., Mutton A., Day C. P. - Diagnosis and interpretation of steatosis and
C
Canistro D., Barillari J., Melega S., Sapone A., Iorib R., Speronia E., Paolin M. - Black
cabbage seed extract affects rat Cyp-mediated biotransformation: Organ and sex related differences. Food Chem Toxicol. 2012, 50: 2612-2621.
Caro A. A., Cederbaum A.I. - Oxidative stress, toxicology and pharmacology
of CYP2E1. Annu Rev Pharmacol Toxicol. 2004, 44: 27-42
Cartea M. E., Francisco M., Soengas P., Velasco P. - Phenolic compounds in Brassica
vegetables. Molecules. 2011; 16: 251-280.
Castro-Torres I. G., Naranjo-Rodriguez, Dominguez-Ortiz M. A., Gallegos-Estudillo J., Saavedra-Vèlez M. V. - Antilithiasic and Hypolipidaemic Effects of Raphanus sativus L.
var. niger on Mice Fed with a Lithogenic Diet. J Biomed Biotechnol. 2012; 2012: 161205.
Cederbaum A. I. - Molecular mechanisms of the microsomal mixed function oxidases and
biological and pathological implications. Redox Biol. 2015, 4: 60–73.
Cencic A., Chingwaru W.- The role of functional foods, nutraceuticals, and food
supplements in intestinal health. Nutrients. 2010, 2: 611-625
Charradi K., Mahmoudi M., Bedhiafi T., Kadri S., Elkahoui S., Limam F., Aouani E. -
Dietary supplementation of grape seed and skin flour mitigates brain oxidative damage induced by a high-fat diet in rat. Gender dependency. Biomed Pharmacother. 2017, 87:
519-526.
Chaturvedi P. - Inhibitory Response of Raphanus sativus on Lipid Peroxidation in Albino
Rats. Evid Based Complement Alternat Med. 2008, 5: 55–59
Chaturvedi P., Tulsyan S., Agarwal G., Lal P., Agrawal S., Mittal R.D., Mittal B. -
Relationship of MTHFR and NQO1 Pharmacogenetics and Chemotherapy Clinical Outcomes in Breast Cancer Patients. Biochem Genet. 2015, 53: 211-222.
Chen G., Ni Y., Nagata N., Xu L. and Ota T. - Micronutrient Antioxidants and Nonalcoholic
Fatty Liver Disease. Int. J. Mol. Sci. 2016, 17: 1379
Chen Z. Y., Jiao R., Ma K. Y. - Cholesterol- Lowering Nutraceutical and Functional Food. J. Agric. Food Chem. 2008, 56: 8761-8773
Choi K. C., Cho S. W., Kook S. H., Chun S. R., Bhattarai G., Poudel S. B., Kim M. K., Lee K. Y., Lee J. C. - Intestinal anti-inflammatory activity of the seeds of Raphanus sativus L.
in experimental ulcerative colitis models. J Ethnopharmacol. 2016, 179: 55-65.
Cusi K. - Treatment of patients with type 2 diabetes and non-alcoholic fatty liver disease:
Cwik M. J., Wu H., Muzzio M., McCormick D. L., Kapetanovic I. - Direct quantitation of
glucoraphanin in dog and rat plasma by LC-MS/MS. J Pharm Biomed Anal. 2010, 52: 544-
549. D
Dahiya D. K., Renuka, Puniya M., Shandilya U. K., Dhewa T., Kumar N., Kumar S., Puniya A. K., Shukla P. - Gut Microbiota Modulation and Its Relationship with Obesity Using
Prebiotic Fibers and Probiotics: A Review. Front Microbiol. 2017, 8: 563.
Day C. P., James O. F. W. - Steatohepatitis: a tale of two “hits”? Gastroenterology. 1998, 114: 842-845.
DeFelice S. L. - The nutraceutical revolution: its impact on food industry R&D. Trends Food Sci Tech. 1995, 6: 59-61.
Ding S., Chi M. M., Scull B. P., Rigby R., Schwerbrock N. M., Magness S., Jobin C., Lund P. K. - High-fat diet: bacteria interactions promote intestinal inflammation which
precedes and correlates with obesity and insulin resistance in mouse. Plos one. 2010, 5:
e12191
Ding S., Lund P.K. - Role of intestinal inflammation as an early event in obesity and insulin
resistance. Curr Opin Clin Nutr Metab Care. 2011, 14: 328-33.
Dowman J. K., Tomlinson J. W., Newsome P. N. - Pathogenesis of non-alcoholic fatty
liver disease. Q J Med. 2010, 103: 71–83
Dröge W. - Free radicals in the physiological control of cell function. Physiol Rev. 2002, 82: 47-95.
E
Enomoto E., Ikejima K., Bradford B. U., Rivera C. A., Kono H., Goto M., Yamashina S., Schemmer P., Kitamura T., Oide H., Takei Y., Hirose M., Shimizu H., Miyazaki A., Brenner D. A., Sato N., Thurman R. G. - Role of Kupffer cells and gut-derived endotoxins
in alcoholic liver injury. J Gastroenterol Hepatol. 2000, 15: D20-D25.
Eslamparast T., Eghtesad S., Poustchi H., Hekmatdoost A. - Recent advances in dietary
supplementation, in treating non-alcoholic fatty liver disease. World J Hepatol. 2015, 7:
204-212 F
FAO/WHO expert consultation - Health and nutritional properties of probiotics in food
Fahey J. W., Zalcmann A. T., Talalay P. - The chemical diversity and distribution of
glucosinolates and isothiocyanates among plants. Phytochemistry. 2001, 56: 5-51.
Feng W., Wang H., Zhang P., Gao C., Tao J., Ge Z., Zhu D., Bi Y. - Modulation of gut
microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats.
Biochim Biophys Acta. 2017.
Feng Y., Yu Y. H., Wang S. T., Ren J., Camer D., Hua Y. Z., Zhang Q., Huang J., Xue D. L., Zhang X. F., Huang X. F., Liu Y. - Chlorogenic acid protects d-galactose-induced liver and
kidney injury via antioxidation and anti-inflammation effects in mice. Pharm Biol. 2016,
25: 1-8.
Ferruzza S., Natella F., Ranaldi G., Murgia C., Rossi C., Trošt K., Mattivi F., Nardini M., Maldini M., Giusti A., Moneta E., Scaccini C., Sambuy Y., Morelli G. and Baima S. -
Nutraceutical Improvement Increases the Protective Activity of Broccoli Sprout Juice in a Human Intestinal Cell Model of Gut Inflammation. Pharmaceuticals. 2016, 9: 48
Fluck C. E., Nicolo C., Pandey A. V. - Clinical, structural and functional implications of
mutations and polymorphisms in human NADPH P450 oxidoreductase. Fundam Clin
Pharmacol. 2007, 21: 399-410.
Folch J., Lees M., Sloane Stanley G. H. - A simple method for the isolation and
purification of total lipids from animal tissue. J Biol Chem. 1957, 226: 497-509.
Fuentes F., Paredes-Gonzalez X., and Kong A. T. - Dietary Glucosinolates Sulforaphane,
Phenethyl Isothiocyanate, Indole-3-Carbinol/3,3′-Diindolylmethane: Anti-Oxidative Stress/Inflammation, Nrf2, Epigenetics/Epigenomics and In Vivo Cancer Chemopreventive Efficacy. Curr Pharmacol Rep. 2015, 1: 179–196.
G
Getahun S. M., Chung F. L. - Conversion of glucosinolates to isothiocyanates in humans
after ingestion of cooked watercress. Cancer Epidemiol Biomarkers Prev. 1999, 8: 447–
51.
Giby V. G., Ajith T. A. - Role of adipokines and peroxisome proliferator-activated
receptors in nonalcoholic fatty liver disease. World J Hepatol. 2014, 6: 570-579.
Girgin N, El N. S. - Effects of cooking on in vitro sinigrin bioaccessibility, total phenols,
antioxidant and antimutagenic activity of cauliflower (Brassica oleraceae L. var. Botrytis). J Food Comp and Anal. 2015, 37: 119-127.
Giovannini C., Filesi C., D’Archivio M., Scazzocchio B., Santangelo C., Masella R. -
Polifenoli e difese antiossidanti endogene: effetti sul glutatione e sugli enzimi ad esso correlati. ANN IST SUPER SANITÀ. 2006, 42: 336-347.
Guengerich F. P. - Cytochrome P450s and other enzymes in drug metabolism and
toxicity. The AAPS J. 2006, 8: E101-111.
Guo L. Q., Yamazoe Y. - Inhibition of cytochrome P450 by furanocoumarins in grapefruit
juice and herbal medicines. Acta Pharmacol Sin. 2004, 25: 129-136.
H
Harishekar M. B. and Kiran B. - Effect of Lead, Alcohol and Vitamin E on Protein carbonyl
content in rats. J Appl Pharmaceut Sci. 2011, 01: 154-156.
Hassan K., Bhalla V., Regal M. E. E., A-Kader H. H. - Nonalcoholic fatty liver disease: A
comprehensive review of a growing epidemic. World J Gastroenterol 2014, 20: 12082-
12101
Hegazy A. M., Abdel Azeem A. S., Shahy E. M., El-Sayed E. M. - Comparative study of
cholinergic and oxidative stress biomarkers in brains of diabetic and hypercholesterolemic rats. Hum Exp Toxicol. 2016, 35: 251-258.
Hissin P. J., Hilf R. - A Fluorometric Method for Determination of Oxidized and Reduce
Glutathione in Tissues. Anal Biochem. 1976, 74: 214-226.
Holst B., Williamson G. - A critical review of the bioavailability of glucosinolates and
related compounds. Nat Prod Rep. 2004, 21: 425-447.
Holt P.R., Katz S., Kirshoff R. - Curcumin therapy in inflammatory bowel disease: a pilot
study. Dig Dis Sci 2005; 50:2191-2193.
Holtzclaw W. D., Dinkova-Kostova A.T., Talalay P. - Protection against electrophile and
oxidative stress by induction of phase 2 genes: the quest for the elusive sensor that responds to inducers. Adv Enzyme Regul. 2004, 44: 335-367.
Hruszkewycz A. M. - Evidence for mitochondrial DNA damage by lipid peroxidation. Biochem Biophys Res Commun. 1988, 153: 191-197.
Huang D., Ou B., Prior R.L. - The chemistry behind antioxidant capacity assays. J. Agric Food Chem. 2005, 53: 1841-1856.
I
Ichimura M., Kawase M., Masuzumi M., Sakaki M., Nagata Y., Tanaka K., Suruga K., Tamaru S., Kato S., Tsuneyama K., Omagari K. - High-fat and high-cholesterol diet
rapidly induces non-alcoholic steatohepatitis with advanced fibrosis in Sprague-Dawley rats. Hepatol Res. 2015, 45: 458-469.
Itoh K., Wakabayashi N., Katoh Y., Ishii T., Igarashi K., Engel J. D., Yamamoto M. - Keap1
represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domain. Genes Dev. 1999, 13: 76-86.
J
Jadoun J., Yazbak A., Rushrush S., Rudy A., Azaizeh H. - Identification of a New
Antibacterial Sulfur Compound from Raphanus sativus Seeds. Evid Based Complement
Alternat Med. 2016, 2016: 9271285.
Jiang T., Gao X., Wu C., Tian F., Lei Q., Bi J., Xie B., Wang H. Y., Chen S., Wang X. - Apple-
Derived Pectin Modulates Gut Microbiota, Improves Gut Barrier Function, and Attenuates Metabolic Endotoxemia in Rats with Diet-Induced Obesity. Nutrients. 2016,
8: 126.
Joseph P., Long D. J., Klein-Szanto A. J., Jaiswal A. K. - Role of NAD(P)H:quinone
oxidoreductase 1 (DT diaphorase) in protection against quinone toxicity. Biochem
Pharmacol. 2000, 60: 207-214. K
Kalra E. K. - Nutraceutical-definition and introduction. AAPS PharmSci. 2003, 5: 25. Kakizaki T., Kitashiba H., Zou Z., Li F., Fukino N., Ohara T., Nishio T., and Ishida M.- A 2-
Oxoglutarate-Dependent Dioxygenase Mediates the Biosynthesis of Glucoraphasatin in Radish. Plant Physiol. 2017, 173: 1583–1593.
Kaulmann A., Jonville M. C., Schneider Y. J., Hoffmann L., Bohn T. - Carotenoids,
polyphenols and micronutrient profiles of Brassica oleraceae and plum varieties and their contribution to measures of total antioxidant capacity. Food Chem. 2014, 155: 240-250.
Kawano M., Miyoshi M., Ogawa A., Sakai F., Kadooka Y. - Lactobacillus gasseri SBT2055
inhibits adipose tissue inflammation and intestinal permeability in mice fed a high-fat diet. J Nutr Sci. 2016, 5: e23.
Khan I., Samson S. E. and Grover A. K. - Antioxidant Supplements and Gastrointestinal
Diseases: A Critical Appraisal. Med Princ Pract. 2017, 26: 201-217.
Kim K. A., Gu W., Lee I. A., Joh E. H., Kim D. H. - High Fat Diet-Induced Gut Microbiota
Exacerbates Inflammation and Obesity in Mice via the TLR4 Signaling Pathway. PLoS
One. 2012, 7: e47713.
Kim C. S., Kwon Y., Choe S-Y., Hong S-M., Yoo H., Goto T., Kawada T., Choi H.-S., Joe Y., Chung H. T., Yu R. - Quercetin reduces obesity-induced hepatosteatosis by enhancing
mitochondrial oxidative metabolism via heme oxygenase-1. Nutr Metab. 2015, 12: 33.
Kirpich A. I., Marsano L. S., M. D., McClain C. J. - Gut-Liver Axis, Nutrition, and Non
Ko I. Y., Park S. S., Song B. J., Patten C., Tan Y. Z., Hah Y. C., Yang C. S., Gelboin H. V. -
Monoclonal antibodies to ethanol-induced rat liver cytochrome P-450 that metabolizes aniline and nitrosamines. Cancer Res. 1987, 47: 3101-3109.
Kobayashi M., Yamamoto M. - Nrf2-Keap 1 regulation of cellular defense mechanisms
against electrophiles and reactive oxygen species. Adv Enzyme Regul. 2006, 46: 113-140.
Kondamudi P. K., Kovelamudi H., Nayak P. G., Rao M.C., Shenoy R. R. - Curcumin half
analog modulates interleukin-6 and tumor necrosis factor-alpha in inflammatory bowel disease. Pharmacogn Mag. 2015, 11: S296-S302.
Kucera O., Cervinkova Z. - Experimental models of non-alcoholic fatty liver disease in
Rats. World J Gastroenterol. 2014, 20: 8364-8376.
Kurilich A. C., Tsau G. J., Brown A., Howard L., Klein B. P., Jeffery E. H., Kushad M., Wallig M. A., Juvik J. A. - Carotene, tocopherol, and ascorbate contents in subspecies of
Brassica oleracea. J Agric Food Chem. 1999, 47: 1576–1581.
Kuzu N., Bahcecioglu I. H., Dagli A. F., Ozercan I. H., Ustundag B., Sahin K. -
Epigallocatechin gallate attenuates experimental non-alcoholic steatohepatitis induced by high fat diet. J Gastroenterol Hepatol. 2008, 23: e465-70.
L
Lampe J. W., Peterson S. - Brassica, biotransformation and cancer risk: genetic
polymorphisms alter the preventive effects of cruciferous vegetables. J Nutr. 2002, 132:
2991-4.
Larter C. Z., Chitturi S., Heydet D., Farrell G. C. - A fresh look at NASH pathogenesis. Part
1: the metabolic movers. J Gastroenterol Hepatol. 2010, 25: 672-690.
Lee S. J., Yoo J. D., Choi S. Y., Kwon O. S. - the expression and secretion of vimentin in
the progression of non alcolic steatohepatitis. BMB Rep. 2014, 47: 457-462.
Li F., Hullar M. A. J., Beresford S. A. A., Lampe J. W. - Variation of glucoraphanin
metabolism in vivo and ex vivo by human gut bacteria. Br J Nutr. 2011, 106: 408–416.
Li J., Sapper T. N., Mah E., Rudraiah S. Schill K. E., Chitchumroonchokchai C., Moller M. V., McDonald J. D., Rohrer P. R., Manautou J. E., Bruno R. S. - Green tea extract provides
extensive Nrf2-independent protection against lipid accumulation and NFκB pro- inflammatory responses during nonalcoholic steatohepatitis in mice fed a high-fat diet.
Mol Nutr Food Res. 2016, 60: 858-70.
Liang N., Kitts D. D. - Role of Chlorogenic Acids in Controlling Oxidative and Inflammatory
Lieber C. S., Leo M. A., Mak K. M., Xu Y., Cao Q., Ren C., Ponomarenko A., Decarli L. M. - Model of nonalcoholic steatohepatitis. Am J Clin Nutr. 2004, 79: 502-509.
Liu R. H. - Health benefits of fruit and vegetables are from additive and synergistic
combinations of phytochemicals. Am J Clin Nutr. 2003, 78: 517S-520S
Longo V., Mazzaccaro A., Naldi F., Gervasi P.G. - Drug-metabolizing enzymes in liver,
olfactory, and respiratory epithelium of cattle. J Biochem Toxicol. 1991, 6: 123-128.
Lowry O. H., Rosebrough N.J., Farr A.L., Randall R.J. - Protein measurement with the
Folin phenol reagent. J Biol. Chem. 1951, 193: 265-275.
M
Machado M. V., Pinto H. C. - Diet, Microbiota, Obesity, and NAFLD: A Dangerous
Quartet. Int. J. Mol. Sci. 2016, 17: 481.
Mark M., Teletin M., Antal C., Wendling O., Auwerx J., Heikkinen S., Khetchoumian K., Argmann C. A., Dgheem M. - Histopathology in mouse metabolic investigations current
protocols in molecular biology. Curr Protoc Mol Biol. 2007, 29B.4.1 - 29B.4.32
Martins M. V., Montezano de Carvalho I. M., Magalhães Caetano M. M., Lopes Toledo R. C., Avelar Xavier A., De Queiroz J. H. - Neuroprotective effect of Sapucaia nuts
(Lecythis pisonis)on rats fed with high-fat diet. Nutr Hosp. 2016, 33: 1424-1429.
Melega S., Canistro D., Pagnotta E., Iori R., Sapone A., Paolini M. - Effect of sprout
extract from Tuscan black cabbage on xenobiotic-metabolizing and antioxidant enzymes in rat liver. Mutat Res. 2013, 751: 45-51.
Melega S., Canistro D., De Nicola G. R., Lazzeri L., Sapone A., Paolini M. - Protective
effect of Tuscan black cabbage sprout extract against serum lipid increase and perturbations of liver antioxidant and detoxifying enzymes in rats fed a high-fat diet. Br
J Nutr. 2013, 110: 988-997.
Mercier Y., Gatellier P., Renerre M. - Lipid and protein oxidation in vitro, and antioxidant
potential in meat from Charolais cows finished on pasture or mixed diet. Meat Sci. 2004,
66: 467-473.
Mizuno N., Niwa T., Yotsumoto Y., Sugiyama Y. - Impact of drug transporter studies on
drug discovery and development. Pharmacol Rev. 2003, 55: 425-461.
Mnafgui K., Hamden K., Ben Salah H., Kchaou M., Nasri M., Slama S., Derbali F., Allouche N., Elfeki A. - Inhibitory Activities of Zygophyllum album: A Natural Weight-
Lowering Plant on Key Enzymes in High-Fat Diet-Fed Rats. Evid Based Complement
Moos W. H., Faller D. V., Harpp D. N., Kanara I., Pernokas J., Powers W. R., Steliou K. -
Microbiota and Neurological Disorders: A Gut Feeling. Biores Open Access. 2016, 5: 137-
45.
Mota M., Bubu A., Banini B. A., Sophie C., Cazanave S. C., Sanyal A. J. - Molecular
Mechanisms of Lipotoxicity and Glucotoxicity in Nonalcoholic Fatty Liver Diseas.
Metabolism. 2016, 65: 1049–1061
Murphy E. A., Velazquez K. T., Herbert K. M. - Influence of High-Fat-Diet on Gut
Microbiota: A Driving Force for Chronic Disease Risk. Curr Opin Clin Nutr Metab Care.
2015, 18: 515–520. N
Naughton P., Foresti R., Bains S. K., Hoque M., Green C. J., Motterlini R. - Induction of
Heme Oxygenase-1 by nitrosative stress. A role for nitroxyl anion. J Biol Chem. 2002, 277:
40666-40674.
Nobili V., Alkhouri N., Alisi A., Della Corte C., Fitzpatrick E., Raponi M., Dhawan A. -
Nonalcoholic fatty liver disease: a challenge for pediatricians. JAMA Pediatr. 2015, 169:
170-176. O
Ohkawa H., Ohishi N., Yagi K. - Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979, 95: 351-358.
Orellana M., Guajardo V. - Cytochrome P450 activity and its alteration in different
diseases. Rev Med Chil. 2004, 132: 85-94.
Ozcan U., Cao Q., Yilmaz E., Lee A. H., Iwakoshi N. N., Ozdelen E., Tuncman G., Gorgun C., Glimcher L. H., Hotamisligil G. S. - Endoplasmic reticulum stress links obesity, insulin
action and type 2 diabetes. Science. 2004, 306: 457-461.
P
Paolini M., Nestle M. - Pitfalls of enzyme-based molecular anticancer dietary
manipulations: food for thought. Mutat Res. 2003, 543: 181-189.
Park H. J., Lee J. Y., Chung M. Y., Park Y. K., Bower A. M., Koo S. I., Giardina C., Bruno R. S. - Green tea extract suppresses NFκB activation and inflammatory responses in diet-
induced obese rats with nonalcoholic steatohepatitis. J Nutr. 2012, 142: 57-63.
Parkinson E. I., Bair J. S., Cismesia M., Hergenrother P. J. - Efficient NQO1 substrates
Phaniendra A., Jestadi D. B., Periyasamy L. - Free radicals: properties, sources, targets,
and their implication in various diseases. Indian J Clin Biochem. 2015, 30: 11-26.
Porter T. D., Coon M. J. - Cytochrome P-450. multiplicity of isoforms,substrates, and
catalytic and regulatory mechanisms. J Biol Chem. 1991, 266: 13469-13472.
Pozzo L., Vornoli A., Coppola I., Della Croce C. M., Giorgetti L., Gervasi P. G., Longo V. - Effect of HFD/STZ on expression of genes involved in lipid, cholesterol and glucose
metabolism in rats. Life Sci. 2016, 166: 149-156.
R
Raish M., Ahmad A., Alkharfy K. M., Ahamad S. R., Mohsin K., Al-Jenoobi F. I., Al- Mohizea A. M., Ansari M. A. - Hepatoprotective activity of Lepidium sativum seeds
against D-galactosamine/lipopolysaccharide induced hepatotoxicity in animal model.
BMC Complement Altern Med. 2016, 16: 501.
Rayan D. E., Lewin W. - Purification and characterization of hepatic microsomal
cytochrome P-450. Pharmacol Ther. 1990, 45: 153-239.
Raunio H., Kuusisto M., Juvonen R. O., Pentikainen O. T. - Modeling interactions
between xenobiotics and cytochrome P450 (CYP) enzimes. Front Pharmacol. 2015, 6:
123.
Reed M. J., Meszaros K., Entes L. J., Claypool M. D., Pinkett J. G., Gadbois T. M., and Reaven G. M. - A New Rat Model of Type 2 Diabetes: The Fat-Fed, Streptozotocin-Treated
Rat. Metabolism. 2000, 49: 1390-1394.
Reddy J. K., Rao M. S.- Lipid Metabolism and Liver Inflammation. II. Fatty liver disease
and fatty acid oxidation. Am J Physiol Gastrointest Liver Physiol. 2006, 290: G852-G858.
Reinke L. A., Moyer M. J. - p-Nitrophenol hydroxylation. A microsomal oxidation which
is highly inducible by ethanol. Drug Metab Dispos. 1985, 13: 548-552.
Resta S. C. - Effects of probiotics and commensals on intestinal epithelial physiology:
implications for nutrient handling. J Physiol. 2009, 587: 4169–4174.
Rice-Evans C. A. - Plant plyphenols: free radical scavangers or chain breaking
antioxidants? Biochem Soc Symp. 1995; 61: 103-116.
Rodas M. C. H., Valenzuela R., Videla L. A. - Relevant Aspects of Nutritional and Dietary
Interventions in Non-Alcoholic Fatty Liver Disease. Int. J. Mol. Sci. 2015, 16: 25168-
25198.
Ross D., Beall H., Traver R. D., Siegel D., Phillips R. M., Gibson N. W. - Bioactivation of
quinones by DT-diaphorase, molecular, biochemical, and chemical studies. Oncol Res.
Rungapamestry V., Duncan A. J., Fuller Z., Ratcliffe B. - Effect of cooking brassica
vegetables on the subsequent hydrolysis and metabolic fate of glucosinolates. Proc Nutr
Soc. 2007, 66: 69-81. S
Saliu J. A., Oboh G., Omojokun O. S., Rocha J. B., Schetinger M. R., Guterries J., Stefanello N., Carvalho F., Schmatz R., Morsch V. M., Boligon A. - Effect of dietary
supplementation of Padauk (Pterocarpus soyauxii) leaf on high fat diet/streptozotocin induced diabetes in rats' brain and platelets. Biomed Pharmacother. 2016, 84: 1194-
1201.
Santamarina A. B., Oliveira J. L., Silva F. P., Carnier J., Mennitti L. V., Santana A. A., de Souza G. H., Ribeiro E. B., Oller do Nascimento C. M., Lira F. - Green Tea Extract Rich in
Epigallocatechin-3-Gallate Prevents Fatty Liver by AMPK Activation via LKB1 in Mice Fed a High-Fat Diet. PLoS One. 2015, 10: e0141227.
Sapone A., Canistro D., Melega S., Moles R., Vivarelli F., and Paolini M. - On Enzyme-
Based Anticancer Molecular Dietary Manipulations. J Biomed Biotechnol 2012, 2012:
790987.
Scalbert A., Williamson G. - Dietary intake and bioavailability of polyphenols. J Nutr. 2000, 130: 2073S-2085S
Schieber M., Chandel N. S. - ROS function in redox signaling and oxidative stress. Curr Biol. 2014, 24: R453-R462.
Schlager J. J., Powis G. - Cytosolic NAD(P)H:(quinone-acceptor) oxidoreductase in human
normal and tumor tissue: effects of cigarette smoking and alcohol. Int J Cancer. 1990,
45: 403-409.
Seljeskog E., Hervig T., Mansoor M. A. - A novel HPLC method for the measurement of
thiobarbituric acid reactive substances (TBARS). A comparison with a commercially available kit. Clin Biochem. 2006, 39: 947-954.
Shapiro H., Singer P., Halpern Z., Bruck R. - Polyphenols in the treatment of
inflammatory bowel disease and acute pancreatitis. Gut. 2007, 56: 426‑435.
Shapiro T. A., Fahey J. W., Wade K. L., Stephenson K. K., Talalay P. - Chemoprotective
glucosinolates and isothiocyanates of broccoli sprouts: metabolism and excretion in humans. Cancer Epidemiol Biomarkers Prev. 2001, 10: 501-508.
Singh J., Sinha S. - Classification, regulatory acts and applications of nutraceuticals for
Singh R. K., Chang H. W., Yan D., Lee K. M., Ucmak D., Wong K., Michael Abrouk M., Farahnik B., Nakamura M., Zhu T. H., Bhutani T. and Liao W. - Influence of diet on the
gut microbiome and implications for human health. J Transl Med. 2017, 15: 73.
Slavin J. L. and Lloyd B. - Health Benefits of Fruits and Vegetables. Adv. Nutr. 2012, 3: 506–516.
Smine S., Obry A., Kadri S., Hardouin J., Fréret M., Amri M., Jouenne T., Limam F., Cosette P., Aouani E. - Brain proteomic modifications associated to protective effect of
grape extract in a murine model of obesity. Biochim Biophys Acta. 2017, 1865: 578-588.