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Bioinformatic identification of a putative microRNA-transcription factor network motif in the regulation of laccase genes in peach (Prunus persica)

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6thRosaceousGenomicsConference

Mezzocorona,Italy–30thSeptemberͲ04thOctober2012    ͺͷ  ȋͷȌ ƒ…—Ž–ƒ††‡‹‡…‹ƒ•‰”‘×‹…ƒ•ǡ‡’ƒ”–ƒ‡–‘†‡”‘†—……‹×‰”À…‘Žƒǡƒ„‘”ƒ–‘”‹‘ †‡‡×‹…ƒ —…‹‘ƒŽƬ‹‘‹ˆ‘”ž–‹…ƒǡ‹˜‡”•‹†ƒ††‡Š‹Ž‡ǡƒ–‹ƒ‰‘ǡŠ‹Ž‡  Š‹Ž‡‹•–Š‡ƒ‹‡š’‘”–‡”‘ˆ ƒ’ƒ‡•‡’Ž—ˆ”‡•Šˆ”—‹–‹–Š‡™‘”Ž†Ǥ‡‘ˆ–Š‡‡›‹••—‡• ˆ‘” –Š‡ ‹†—•–”› ‹• –Š‡ ’‘•– Šƒ”˜‡•– …‘†‹–‹‘• ‘ˆ –Š‡ ˆ”—‹–• –Šƒ– ƒŽŽ‘™ ”‡ƒ…Š‹‰ –Š‡ ƒ”‡– ™‹–Š ƒ ‰‘‘† “—ƒŽ‹–› ’”‘†—…–Ǥ Š‡ ˆ”—‹–• ƒ”‡ Š‹‰ŠŽ› ’‡”‹•Šƒ„Ž‡ ƒ† •—ˆˆ‡” ’Š›•‹‘Ž‘‰‹…ƒŽ†‹•‘”†‡”•ƒ••‘…‹ƒ–‡†–‘–Š‡Ž‘‰–‹‡•–‘”ƒ‰‡—†‡”Ž‘™–‡’‡”ƒ–—”‡Ǥ‡ ‘ˆ –Š‡•‡ †‹•‘”†‡”• ‹• –Š‡ –‡”ƒŽ ”‡ƒ†‘™ ȋ ȌǤ ‘™ƒ”† –Š‡ ‹†‡–‹ˆ‹…ƒ–‹‘ ‘ˆ ‰‡‡• †‹ˆˆ‡”‡–‹ƒŽŽ› ‡š’”‡••‡† ”‡Žƒ–‡† –‘  ‹ ’Ž— ˆ”—‹–ǡ ƒ •—„–”ƒ…–‹˜‡ •—’’”‡••‹˜‡ Š›„”‹†‹œƒ–‹‘ ȋ Ȍ ‡–Š‘†‘Ž‘‰› ™ƒ• ‡’Ž‘›‡†Ǥ ™‘ Ž‹„”ƒ”‹‡• ™‡”‡ ‰‡‡”ƒ–‡† ˆ”‘ –”ƒ•…”‹’–•‘„–ƒ‹‡†ˆ”‘†‹ˆˆ‡”‡–•–‘”ƒ‰‡–”‡ƒ–‡–•Ǥ•‡–‘ˆ͵ͺ͹‰‡‡•™‡”‡‹•‘Žƒ–‡†ǡ •‡“—‡…‡†ƒ†…Šƒ”ƒ…–‡”‹œ‡†ǤƒŽ‰‘”‹–Šƒ†’—„Ž‹…†ƒ–ƒ„ƒ•‡•ȋ ƒ† Ȍ ™‡”‡ —•‡† –‘ †‡–‡”‹‡ •‹‹Žƒ”‹–› •…‘”‡• „‡–™‡‡ –Š‡ … …Ž‘‡• ƒ† ‘™ •‡“—‡…‡•Ǥ —…–‹‘ƒŽ …ƒ–‡‰‘”‹œƒ–‹‘ ™ƒ• ƒ—ƒŽŽ› †‡–‡”‹‡† —•‹‰ –Š‡ —ƒ– …ƒ–ƒŽ‘‰—‡ ˆ”‘ –Š‡ —‹…Š ˆ‘”ƒ–‹‘ ‡–‡” ˆ‘” ”‘–‡‹ ‡“—‡…‡• ȋ Ȍ Ǥ ’’”‘š‹ƒ–‡Ž› ͻͲΨ ‘ˆ –Š‡ ’ƒ”–‹ƒŽ …• •Š‘™‡† •‹‰‹ˆ‹…ƒ– •‹‹Žƒ”‹–› –‘ ’”‘–‡‹• ”‡‰‹•–‡”‡†‹†ƒ–ƒ„ƒ•‡•ƒ†ͳͲΨ’”‡•‡–‡†’”‘„Ž‡•™‹–Š–Š‡•‡“—‡…‡–Š‡”‡ˆ‘”‡™‡”‡ ‘–…‘•‹†‡”‡†ˆ‘”–Š‡ƒƒŽ›•‹•Ǥ”‘–‡‹•”‡Žƒ–‡†–‘’”‘–‡‹ˆƒ–‡ȋˆ‘Ž†‹‰ǡ‘†‹ˆ‹…ƒ–‹‘ǡ †‡•–‹ƒ–‹‘Ȍ™‡”‡‹†‡–‹ˆ‹‡†™Š‡ƒ–”‡ƒ–‡–‘ˆ–™‘†ƒ›•ƒ–ʹͲ͑ƒ†•–‘”ƒ‰‡ƒ–Ͳ͑ ˆ‘”Ͷʹ†ƒ›•ȋˆ”—‹–Ž‹„”ƒ”›ʹȌ™ƒ•—•‡†Ǥ‘”‡‘˜‡”ǡ‰‡‡•–Šƒ–…‘†‹ˆ›„›’”‘–‡‹•”‡Žƒ–‡† –‘…‡ŽŽ—Žƒ”–”ƒ•’‘”–ǡ…‡ŽŽǦ™ƒŽŽǦ”‡Žƒ–‡†’”‘–‡‹•ƒ†–”ƒ•…”‹’–‹‘ˆƒ…–‘”•™‡”‡‹†‡–‹ˆ‹‡† ƒˆ–‡”ƒ–”‡ƒ–‡–‘ˆ–™‘†ƒ›•ƒ–ͳͲ͑ƒ†•–‘”ƒ‰‡ƒ–Ͳ͑ˆ‘”Ͷʹ†ƒ›•ȋˆ”—‹–Ž‹„”ƒ”›ͶȌǤ ‘—”‰‡‡•™‡”‡ƒƒŽ›œ‡†„›“ǦǤ Š‹• ™‘” ™ƒ• •—’’‘”–‡† „› ˆ‡ŽŽ‘™•Š‹’ ‘‹…›– ʹͳͲͻͲͳͺǡ ‡•‹• †‡ ‘•–‰”ƒ†‘ ‡ Žƒ †—•–”‹ƒ͹ͺͳͳͳͳͳʹǡ’”‘Œ‡…– ‘†‡…›–ͳͳͲͺͲͳͻʹƒ†‘•‘…‹‘‡…‘Ž×‰‹…‘†‡Žƒ ”—–ƒ Ǥ 

Bioinformatic identification of a putative micrornaǦtranscription factor network motifintheregulationoflaccasegenesinpeach(Prunuspersica)  A.M.Cattaneo(1Ȍǡ”œ‡•‘™‹ƒǤǤ(1Ȍǡ‘Žƒ‹ƒ…‘˜‘Ǥ(2Ȍǡ ƒ……‹‘Ž‹Ǥ(2Ȍ  ȋͳȌ‡•‡ƒ”…Šƒ† ‘˜ƒ–‹‘‡–”‡Ǧ ‘†ƒœ‹‘‡†—†ƒ…Šǡƒ‹…Š‡Ž‡ƒŽŽǯ†‹‰‡ ȋȌǡ –ƒŽ› ȋʹȌǦ‡‘‹…•‡•‡ƒ”…Š‡–”‡ǡ ‹‘”‡œ—‘Žƒ†ǯ”†ƒȋȌǡ –ƒŽ›  ƒ……ƒ•‡’”‘–‡‹•ƒ”‡—Ž–‹…‘’’‡”‰Ž›…‘’”‘–‡‹‘š‹†ƒ•‡•‡š’”‡••‡†‹’Žƒ––‹••—‡•—†‡” „‹‘–‹…ƒ†ƒ„‹‘–‹…•–”‡••…‘†‹–‹‘•ǤŠ‡›ƒ”‡ƒ„Ž‡–‘…ƒ–ƒŽ›œ‡‘š‹†ƒ–‹‘‘ˆƒ„”‘ƒ†”ƒ‰‡ ‘ˆ•—„•–”ƒ–‡•‹…Ž—†‹‰’Š‡‘Ž•ƒ†ƒ‹‡•ǤŠ‡”‡‰—Žƒ–‹‘‘ˆ‡š’”‡••‹‘‘ˆ•—…Š‰‡‡• ‹•…”—…‹ƒŽˆ‘”’”‘’‡””‡ƒ…–‹‘–‘•–”‡••Ǥ––Š‡Ž‡˜‡Žǡ–Š‹•‘†—Žƒ–‹‘‹•‡†‹ƒ–‡†„› –Š‡ ”‡…”—‹–‡– ‘ˆ •’‡…‹ˆ‹… –”ƒ•…”‹’–‹‘ ˆƒ…–‘”• ȋ Ȍ –‘ •—‹–ƒ„Ž‡ –”ƒ•…”‹’–‹‘ ˆƒ…–‘” „‹†‹‰ •‹–‡• ȋ Ȍǡ —•—ƒŽŽ› Ž‘…ƒ–‡† —’•–”‡ƒ ‘ˆ ƒ ‰‡‡Ǥ – –Š‡  Ž‡˜‡Žǡ –Š‡ •Š‘”– ‹…”‘•‘Ž‡…—Ž‡•ȋ‹•Ȍ‹–‡”ˆ‡”‡™‹–Š–Š‡–”ƒ•Žƒ–‹‘‘ˆ–ƒ”‰‡–’”‘–‡‹•–Š”‘—‰Š „ƒ•‡Ǧ’ƒ‹”‹‰ ™‹–Š ‡••‡‰‡” •Ǥ ‘’Ž‡š ”‡‰—Žƒ–‘”› …‹”…—‹–• …‘„‹‹‰ –Š‘•‡ ‹–‡”ƒ…–‹‘•ˆ‹‡Ǧ–—‡’”‘–‡‹‡š’”‡••‹‘ƒ†‡Šƒ…‡’Žƒ–”‡•’‘•‡•–‘‡˜‹”‘‡–ƒŽ …Šƒ‰‡Ǥ

(2)

6thRosaceousGenomicsConference

Mezzocorona,Italy–30thSeptemberͲ04thOctober2012    ͺ͸   –Š‹• …ƒ•‡ •–—†› ™‡ ’‡”ˆ‘”‡† ƒ ’Š›Ž‘‰‡‡–‹… ƒƒŽ›•‹• ‘ˆ ’‡ƒ…Š Žƒ……ƒ•‡• ƒ† …Šƒ”ƒ…–‡”‹œ‡†•’‡…‹ˆ‹…’‡ƒ…Š‹•ȋ‹͵ͻ͹ƒƒ†‹ͶͲͺȌǡ”‡’‘”–‡†’”‡˜‹‘—•Ž›ƒ•’‘•–Ǧ –”ƒ•…”‹’–‹‘ƒŽ”‡‰—Žƒ–‘”›‡Ž‡‡–•‘ˆŽƒ……ƒ•‡‰‡‡•Ǥ•‹‰ƒ„‹‘‹ˆ‘”ƒ–‹…ƒ’’”‘ƒ…Š™‡ ‹†‡–‹ˆ‹‡†—‹“—‡ ˆ‘”ƒ„•…‹•‹…ƒ…‹†ȋȌ”‡•’‘•‡‡Ž‡‡–•‹’”‘‘–‡””‡‰‹‘•‘ˆ „‘–Š ‹ ƒ† Žƒ……ƒ•‡ ‰‡‡•Ǥ Š‡ •‹‰ƒŽ‹‰ ‘Ž‡…—Ž‡  ’Žƒ›• ƒ ƒŒ‘” ”‘Ž‡ ‹ ’Žƒ– ”‡•’‘•‡• –‘ •–”‡••Ǥ ‡ ’”‘’‘•‡ ƒ ˆ‡‡†Ǧˆ‘”™ƒ”† Ž‘‘’ ‘–‹ˆ ‹ –Š‡ •–”‡•• ”‡•’‘•‡ ‡–™‘”‹˜‘Ž˜‹‰ƒ…–‹‘‹’‡ƒ…Š„›‹–‡‰”ƒ–‹‰–Š‡ Ǧ‡†‹ƒ–‡†”‡‰—Žƒ–‹‘‘ˆ‹ ƒ†Žƒ……ƒ•‡‰‡‡•ƒ––Š‡–”ƒ•…”‹’–‹‘ƒŽŽ‡˜‡Ž™‹–Š–Š‡‹”‡‰—Žƒ–‹‘‘ˆŽƒ……ƒ•‡–ƒ”‰‡– ‰‡‡•ƒ––Š‡’‘•–Ǧ–”ƒ•…”‹’–‹‘ƒŽŽ‡˜‡ŽǤ  Geneticdissectionoffruitaromainapple  F.Costa(1Ȍ,—‡ƒ Ǥ(2Ȍǡƒ’’‡ŽŽ‹Ǥ(1Ȍǡ‘‰Š‹Ǥ(1ȌǡŽ”‹…ŠǤ(2Ȍǡ‘—‘—Ž‹•Ǥ(1Ȍǡ‡Žƒ•…‘ Ǥ(1Ȍǡƒ•’‡”‹ Ǥ(1Ȍǡ‹ƒ•‹‘Ž‹ Ǥ(1Ȍ  ȋͳȌ‡•‡ƒ”…Šƒ† ‘˜ƒ–‹‘‡–”‡ǡ ‘†ƒœ‹‘‡†—†ƒ…Šǡƒ‹…Š‡Ž‡ƒŽŽǯ†‹‰‡ ȋ”‡–‘Ȍǡ –ƒŽ› ȋʹȌ —Ž‹—•òŠǦ •–‹–—–‡ȋ  Ȍǡ ‡†‡”ƒŽ‡•‡ƒ”…Š‡–”‡ˆ‘”—Ž–‹˜ƒ–‡†Žƒ–•ǡ —‡†Ž‹„—”‰ǡ‡”ƒ›  Š‡ “—ƒŽ‹–› ‘ˆ ƒ ˆ”—‹– ‹• ”‡’”‡•‡–‡† „› ƒ •’‡…‹ˆ‹… •‡– ‘ˆ ’”‹ƒ”› ƒ† •‡…‘†ƒ”› ‡–ƒ„‘Ž‹–‡• ™Š‹…Š ƒ‡ –Š‡ ˆ”—‹– ‡†‹„Ž‡ ƒ† †‡•‹”ƒ„Ž‡Ǥ Š‡ ƒ”‘ƒ–‹… Dz„‘—“‡–dzǡ ‹ ’ƒ”–‹…—Žƒ”ǡ…‘–”‹„—–‡•–‘–Š‡ˆ‹ƒŽƒ’’”‡…‹ƒ–‹‘„›…‘•—‡”•ǡ„‡‹‰–Š‡Žƒ•–ˆƒ…–‘”‘Ǧ †‡•–”—…–‹˜‡Ž›’‡”…‡‹˜‡†ƒˆ–‡”ƒ’’‡ƒ”ƒ…‡ǤŠ‡ƒ”‘ƒ‹ƒ’’Ž‡‹•…‘–”‘ŽŽ‡†„›ƒ•‡”‹‡•‘ˆ ‹’‘”–ƒ–’Š›•‹‘Ž‘‰‹…ƒŽ’ƒ–Š™ƒ›•Ž‡ƒ†‹‰–‘–Š‡ˆ‘”ƒ–‹‘‘ˆ‡›…‘’‘—†•ǡ•—…Šƒ• –‡”’‡‡•ǡƒŽ…‘Š‘Ž•ǡƒŽ†‡Š›†‡•ƒ†‡•–‡”•ǡ–Š‡Žƒ––‡”‘‡–Š‡‘•–ƒ„—†ƒ–‹–Š‡ƒ’’Ž‡ ƒ”‘ƒǤ  ‘”†‡” –‘ ‰‡‡–‹…ƒŽŽ› †‹••‡…– •—…Š …‘’Ž‡š …‘–”‘Žǡ –™‘ ˆ—ŽŽ •‹„ ’”‘‰‡‹‡• ƒ† –™‘ ƒƒŽ›–‹…ƒŽ –‡…Š‘Ž‘‰‹‡• Šƒ˜‡ „‡‡ ‡’Ž‘›‡† ‹ –Š‹• ‹˜‡•–‹‰ƒ–‹‘Ǥ • ’Š‡‘‹…• –‡…Š‘Ž‘‰› ˆ‘” ƒ”‘ƒ ’”‘ˆ‹Ž‹‰ ƒ ‘˜‡Ž Ǧ‘ Ǧ ™ƒ• ‡’Ž‘›‡†ǡ ‹ ‘”†‡” –‘ ˆ‹‰‡”’”‹– –Š‡ • ’”‘†—…–‹‘ ‘ˆ –Š‡ —Œ‹ š ‡Ž‡ƒ”Ž› ’”‘‰‡› ƒˆ–‡” ƒ ’‘•–Šƒ”˜‡•– •–‘”ƒ‰‡ǤŠ‡’”‘ˆ‹Ž‡™ƒ•ˆ—”–Š‡”…‘’ƒ”‡†™‹–Š–Š‡‘‡ƒŽ”‡ƒ†›ƒ˜ƒ‹Žƒ„Ž‡ˆ‘”–Š‡͵ ’‘’—Žƒ–‹‘ȋ‹•…‘˜‡”›š”‹ƒȌǡˆ‘”™Š‹…Šƒ†ƒ–ƒ•‡–‘ˆ˜‘Žƒ–‹Ž‡•ƒ••‡••‡†„› ǦǦ ‡“—‹’‡–™ƒ•ƒŽ”‡ƒ†›ƒ˜ƒ‹Žƒ„Ž‡ǤŠ‡ƒŽ‹‰‡–‘ˆ–Š‡–™‘ƒ’•ƒŽŽ‘™‡†–Š‡†‡–‡…–‹‘ ‘ˆƒ…‘‘•‡–‘ˆ•ǡŠ‹‰ŠŽ‹‰Š–‹‰–Š‘•‡’‘•‹–‹‘‡†‘ʹƒ†…‘ǦŽ‘…ƒ–‡†™‹–Šƒ ‰‡‡…Ž—•–‡”ǡ‘™–‘„‡‹˜‘Ž˜‡†‹ƒ…‡–ƒ–‡‡•–‡”’”‘†—…–‹‘‹ƒ’’Ž‡Ǥ••‘…‹ƒ–‹‘ ƒ’’‹‰ „ƒ•‡† ‘ –Š‡  …ƒ†‹†ƒ–‡ ‰‡‡• ƒŽŽ‘™‡† –Š‡ …Šƒ”ƒ…–‡”‹œƒ–‹‘ ‘ˆ ƒ •‡– ‘ˆ ƒ”‡”• •’‡…‹ˆ‹…ƒŽŽ› ƒ••‘…‹ƒ–‡† ™‹–Š –Š‡ ‡•–‡” ƒ……——Žƒ–‹‘ ‹ ”‹’‡ ˆ”—‹–Ǥ Š‡ ”‡•—Ž–• ’”‡•‡–‡† Š‡”‡ †‹•…—•• ƒ„‘—– –Š‡ —–‹Ž‹–› ‘ˆ –Š‡•‡ ‡™ ƒ”‡”• ƒ• ƒ ˜ƒŽ‹† –‘‘Ž ˆ‘” ƒ ‘Ž‡…—Žƒ”„”‡‡†‹‰–‘™ƒ”†•–Š‡…”‡ƒ–‹‘‘ˆ‡™Š‹‰ŠǦ“—ƒŽ‹–›ƒ”‘ƒ–‹…ƒ’’Ž‡˜ƒ”‹‡–‹‡•Ǥ

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