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Firmato il 11/02/2021 13:35

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Firmato digitalmente da:

COSTA JESSICA

Firmato il 11/02/2021 13:35

Seriale Certificato:

80941106738351767014465678551648208000

Valido dal 11/02/2021 al 11/02/2024

ArubaPEC S.p.A. NG CA 3

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155 Supplementary material

Homogentisic acid and gentisic acid biosynthesized pyomelanin mimics: structural characterization and antioxidant activity

Maher Al-Khatib 1 , Jessica Costa, 1 Daniele Spinelli, Eliana Capecchi 3 , Raffaele Saladino 3 , Maria Camilla Baratto 1 and Rebecca Pogni 1, *

1 Department of Biotechnology, Chemistry and Pharmacy, Via A. Moro 2, 53100 Siena; maher.al@unisi.it, jessica.costa2@unisi.it , mariacamilla.baratto@unisi.it, rebecca.pogni@unisi.it

2 Next Technology Tecnotessile, Via del Gelso, Prato (Italy) 2; chemtech@tecnotex.it

3 Department of Ecology and Biology, University of Tuscia, 01100 Viterbo, Italy; e.capecchi@unitus.it, saladino@unitus.it

* Correspondence: rebecca.pogni@unisi.it;

Scheme 1 Scheme of the radical delocalization in HGA

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Fig S1: Reproducibility test of pyomelanin mimic from homogentisic acid (HGAm). The synthetic conditions were the same for all samples (pH 7.1 and molar ratio lac:HGA 1:1000).

The UV-vis measurements were carried out at room temperature.

Fig S2: Reproducibility test of pyomelanin mimic from gentisic acid (GAm). The synthetic

conditions were the same for all samples (pH 7.1 and molar ratio lac:GA 1:1000). The UV-

vis measurements were carried out at room temperature.

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158

Fig. S5: Room temperature X-band ( = 9.86 GHz) EPR spectra of GAm at pH 7.1 recorded at variable microwave power values.

Fig. S6 Antioxidant activity of HGAm. The EC 50 value was calculated using GraphPad Prism

plotting the DPPH scavenger percentage measured by EPR spectroscopy in function of the

log of HGAm concentrations analyzed. All measurements were repeated in triplicate.

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Fig. S7 Antioxidant activity of GAm. EC 50 value was calculated using GraphPad Prism plotting the DPPH scavenger percentage measured by EPR spectroscopy in function of the log of GAm concentrations analyzed. All measurements were repeated in triplicate.

Fig. S8 Antioxidant activity of gallic acid polymer (GAp). The EC 50 value was calculated

using GraphPad Prism plotting the DPPH scavenger percentage measured by EPR

spectroscopy in function of the log of GAp concentrations analyzed. All measurements were

repeated in triplicate.

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